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AL KAUNIYAH

kauniyah
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Published by Universitas Islam Negeri Syarif Hidayatullah Jakarta

ISSN : 19783736 EISSN : 25026720 DOI : 10.15408/kauniyah

Core Subject : Science,

Biochemistry, Genetics & Molecular Biology Materials Science & Nanotechnology

Al-Kauniyah: Jurnal Biologi (p-ISSN: 1978-3736, e-ISSN: 2502-6720) is an Open Access Journal published by Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islamic University Jakarta, and established since 2007. Since 2016 Al-Kauniyah has established a collaboration with the Association of Lecturer in Biology and Biology Education throughout the State Islamic Higher University (PTKIN) in Indonesia. Until 2015, Al-Kauniyah covered environmental biology solely, but since 2016 the journal has been extended to cover the entire field of biological science (bioscience). By publishing biannually, on April and October, Al-Kauniyah is intended to communicate original researches and current issues on the subject of biology. Since volume 9 issue 1 April 2016, Al-Kauniyah had been changes the layout. This journal warmly welcomes contributions from scholars of related disciplines. Manuscripts can be submitted to AL-KAUNIYAH

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Articles 460 Documents

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PENGARUH PENAMBAHAN DL-METIONIN DALAM MEDIA KULTIVASI TERHADAP PRODUKSI SEFALOSPORIN C DARI KAPANG Acremonium chrysogenum CB2/11/1 Eni Dwi Islamiati; Khaswar Syamsu; Erwahyuni E Prabandani
Al-Kauniyah: Jurnal Biologi Vol 10, No 1 (2017): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Abstrak Sefalosporin merupakan antibiotik β-laktam yang mempunyai efektivitas dalam melawan bakteri Gram positif dan negatif. Antibiotik ini dihasilkan oleh kapang Acremonium chrysogenum. Media kultivasi yang optimum dapat meningkatkan produksi sefalosporin. Komposisi media kultivasi yang paling berpengaruh adalah induser. Induser dapat meningkatkan produksi sefalosporin dari kapang A. chrysogenum. Penelitian ini bertujuan untuk melihat pengaruh metionin dalam media produksi sefalosporin dari kapang A. chrysogenum CB 2/11/1. Inokulum A.chrysogenum dibiakkan pada media kultivasi sefalosporin tanpa penambahan DL-metionin dan pada media kultivasi dengan penambahan DL-metionin. Penentuan level konsentrasi DL-metionin dirancang menggunakan Rancangan Acak Lengkap 1 faktor pada tiap-tiap proses penentuan nutrisi. Data yang diperoleh dari masing-masing perlakuan dianalisis sidik ragam pada taraf nyata 5%. Hasil penelitian menunjukkan bahwa metionin sebagai induser dapat meningkatkan produksi sefalosporin sebesar 1,2 kali dibandingkan dengan media kultivasi yang tanpa menggunakan metionin. Pada pengujian level konsentrasi DL-metionin menunjukkan bahwa konsentrasi terbaik metionin adalah 0,4 g/L menghasilkan sefalosporin sebesar 3989 mg/L. Abstract Cephalosporins is a β-lactam antibiotic that has effectiveness against Gram-positive and negative bacteria. This antibiotic is produced by Acremonium chrysogenum. Optimum cultivation medium can increase the production of cephalosporins. The most influential cultivation medium composition is an inducer that increases production of cephalosporin from the fungi. The objective of this study was to investigate the influence of methionine in the cephalosporin production medium containing A. chrysogenum CB 2/11/1, with DL-methionine as the inducer. The inoculum was cultivated in the cultivation media containing cephalosporins without the addition of DL-metionin and in the media with the addition of DL-metionin. The result showed that the DL-methionine increased cephalosporins production 1.2 fold compared to those without using methionine. The assay on the concentration level of DL-methionine showed the best concentration methionine of 0.4 g/L produced cephalosporin by 3989 mg/L.

Potensi Ekstrak Daun Gulma Babadotan (Ageratum conyzoides L.) terhadap Perkecambahan dan Pertumbuhan Paspalum conjugatum Berg. Mayta Novaliza Isda; Siti Fatonah; Rahmi Fitri
Al-Kauniyah: Jurnal Biologi Vol 6, No 2 (2013): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

The allelopathic substances are an organic agents which produced and released by a plant that cause alteration on the neighboring plants. This research was aimed to determine the effect of Ageratum conyzoides L. leaf extract and also to determine optimum concentration on seed germination and growth of weed Paspalum conjugatum Berg.. This experiment used to extract concentration 0%, 5%, 10%, 15% and 20%. The experiment showed that germination and growth of Paspalum conjugatum Berg.were reduced and to happen damage by leaf extract of Ageratum conyzoides L. The highest germination, growth inhibition and demage at concentrations of the leaf extract 20% respectively are 80.5%, 63.15% dan 17,72 %.

AKTIVITAS TERMOPROTEKSI DAN FOTOPROTEKSI EKSTRAK KASAR KAROTENOID MESOKARP KELAPA SAWIT (Elaeis guineensis Jacq.) TERHADAP KESTABILAN KLOROFIL-A) Dece Elisabeth Sahertian; Indriatmoko Indriatmoko; Leenawaty Limantara; Tatas Hardo Panintingjati Brotosudarmo
Al-Kauniyah: Jurnal Biologi Vol 12, No 1 (2019): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

AbstrakKelapa sawit (Elaeis guineensis Jacq.) merupakan tanaman yang mengandung karotenoid tinggi pada mesokarp yang merupakan bagian dari buah. Cahaya dan suhu dalam proses fotosintesis memberi pengaruh bagi kestabilan dan ketidakstabilan karotenoid dan klorofil. Penelitian ini bertujuan untuk mengetahui aktivitas fotostabilitas dan termostabilitas ekstrak karotenoid mesokarp terhadap klorofil-a yang diiradiasi dengan lampu volpi (intralux 4100) daylight pada intensitas cahaya 31960 lux, 47040 lux dan 76640 lux dalam seri waktu penyinaran 0, 5, 10, 15, 20, 25, dan 30 menit serta dipanaskan pada suhu 25, 50, 65, dan 90 °C dengan seri waktu pemanasan 0, 1, 2, 3, 6, 9, dan 24 jam. Pengukuran spektrum serapan tiap perlakuan diukur menggunakan Spektrofotometer UV-Tampak pada panjang gelombang 300–800 nm. Hasil penelitian menunjukkan bahwa karotenoid tidak melakukan fungsi proteksi dengan baik pada perlakuan pemanasan 65 °C dan 90 °C selama 24 jam, serta perlakuan iradiasi hingga 30 menit pada ketiga intensitas cahaya. Kesimpulan dari penelitian ini adalah klorofil-a murni lebih stabil pada perlakuan pemanasan dan iradiasi. Kemampuan proteksi karotenoid terhadap kestabilan klorofil-a yaitu pada suhu di bawah 50 °C dan pada intensitas cahaya di bawah 31960 lux.Abstract Oil palm (Elaeis guineensis Jacq.) is a plant that contains high carotenoids in its mesocarp which is part of the fruit. Light and temperature in photosynthesis may affect the stability and instability of carotenoid and chlorophyll. The aim of this research was to examine the thermostability and photostability activities of the carotenoids in mesocarp extract on chlorophyll which were irradiated by Volpi lamp (4100 intralux) daylight at light intensity of 31960 lux, 47040 lux and 76640 lux in series of time radiation 0, 5, 10, 15, 20, 25, and 30 minutes and heated at 25, 50, 65, and 90°C with series of heating time 0, 1, 2, 3, 6, 9, and 24 hours. Measurement of the absorption spectrum of each treatment was measured using a UV-Vis Spectrophotometer at a wavelength range of 300–800 nm. The results showed the protective function of carotenoids did not perform well at the heating treatments of 65°C and 90°C in 24 hours and at the irradiation for 30 minutes in all the light intensities. In conclusion, pure chlorophyll-a is more stable in heating and irradiation treatments. Carotenoid protection ability against chlorophyll-a is at temperatures below 50 °C and at a light intensity below 31960 lux.

Induksi Kalus dari Eksplan Daun In Vitro Keladi Tikus (Typhonium sp.) dengan Perlakuan 2,4-D dan Kinetin Marlina Agustina Sitinjak; Mayta Novaliza Isda; Siti Fatonah
Al-Kauniyah: Jurnal Biologi Vol 8, No 1 (2015): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Rodent tuber (Typhonium sp.) belongs to Araceae that has a potential as medicinal plant. This procedure takes a long periode to produce a large quantity of plant. Therefore, an in vitro propagation using callus culture is necessary to solve this problem. This research aimed to determine the best concentration of 2,4-D and kinetin to induce callus of in vitro leaf rodent tuber explants. This research used in vitro leaft explants of rodent tuber with MS medium. The design used is Randomize Group Design with 10 treatment. The results showed that explants could not produce callus, the explants response just a swelling leaf explants. The treatment that could give up to 100% swelling response was 2.4-D 0.5 mg / L dan 1 mg / L and the combination treatment 0.5 mg / L 2.4-D + 0.3 mg /L kinetin, 0.5 mg / L 2.4-D + 0.5 mg / L kinetin and 1.5 mg / L 2.4-D + 0.5 mg / L kinetin. The treatments that could maintain 66.67% explants growth was the combination 0.5 mg / L 2.4-D + 0.5 mg / L kinetin and 1 mg /L 2.4-D + 0.5 mg / L kinetin.

PENGARUH PENAMBAHAN BIOFLOK DENGAN DOSIS BERBEDA TERHADAP PERTUMBUHAN BENIH UDANG WINDU (Penaeus monodon FABRICIUS 1798) Ilham Zulfahmi; Muhammad Syahimi; Muliari Muliari
Al-Kauniyah: Jurnal Biologi Vol 11, No 1 (2018): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

AbstrakPenelitian ini bertujuan untuk mengetahui pengaruh penambahan bioflok terhadap pertumbuhan dan kelangsungan benih udang windu. Penelitian dilakukan selama 30 hari dari Juni hingga Juli 2016 di Laboratorium Program Studi Budidaya Perairan Universitas Almuslim Bireuen. Wadah pemelihara-an berupa akuarium berukuran 50x30x40 cm3 bervolume 10 L dengan padat penebaran 15 ekor per wadah. Penelitian dilaksanakan secara eksperimental menggunakan rancangan acak lengkap dengan empat perlakuan dan tiga ulangan. Perlakuan terdiri atas perlakuan A (kontrol), yaitu tanpa penambahan bioflok, perlakuan B, yaitu penambahan bioflok 10 mL dan 5% pakan komersil, perlakuan C, yaitu penambahan bioflok 15 mL dan 5% pakan komersil, dan perlakuan D, yaitu penambahan bioflok 15 mL dan tanpa penambahan pakan komersil. Parameter yang diamati pada akhir masa pemeliharaan meliputi laju pertumbuhan rata-rata spesifik harian, pertambahan panjang mutlak, dan kelangsungan hidup, yang kemudian diolah dengan menggunakan analisis variansi satu-faktor dengan taraf nyata 0,05. Hasil penelitian menunjukkan bahwa penambahan bioflok berpengaruh nyata terhadap laju pertumbuhan rata-rata spesifik harian dan pertambahan panjang mutlak. Perlakuan B menghasilkan laju pertumbuhan rata-rata spesifik harian tertinggi, yaitu 0,55±0,02% per hari, dan pertambahan panjang mutlak tertinggi, yaitu 3,7±0,1 cm. Namun demikian, penambahan bioflok tidak memberikan pengaruh yang nyata terhadap kelangsungan hidup benih udang windu.AbstractThis research aims to determine the influence of the addition of biofloc on the growth and survival rate of tiger shrimp juvenile. The research was carried out for 30 days from June to July 2016 in the aquaculture laboratory of Almuslim Bireuen University. The maintenance container used was an aquarium with a size of 50x30x40 cm3 that contains 10 L with stocking density 15 tiger shrimp juvenile per aquarium. The research was carried out experimentally using a complete randomized design with four treatments and three replicates. The treatments consist of treatment A (control) which has no addition of biofloc, treatment B which addition of 10 mL biofloc and 5% of commercial feed, treatment C which addition of 15 mL biofloc and 5% commercial feed, and treatment D which addition of 15 mL biofloc without commercial feed. The parameters observed at the end of the maintenance period include daily specific growth rate, absolute length increase, and survival, which were then analyzed by using a one-factor analysis of variance with a significance level of 0.05. The results showed that the addition of biofloc significantly influenced the daily specific growth rate and the absolute length increase. Treatment B gave the highest daily specific growth rate of 0.55±0,02% per day, and the highest absolute length increase of 3.7±0,1 cm. However, the addition of biofloc has no significant influence on the survival of tiger shrimp seeds. Permalink/DOI: http://dx.doi.org/10.15408/kauniyah.v11i1. 4862

POTENSI JAMUR INDIGENUS RIAU (Penicillium sp.PN6) DAN Neptunia oleracea UNTUK BIOREMEDIASI OIL SLUDGE Wahyu Lestari; Atria Martina; Rodesia Mustika Roza; Imelda Wardani
Al-Kauniyah: Jurnal Biologi Vol 11, No 1 (2018): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Abstrak Lumpur minyak bumi (oil sludge) memiliki kandungan kontaminan petroleum hidrokarbon terbesar, karenanya pengolahan limbah oil sludge perlu dilakukan agar tidak mencemari lingkungan. Penelitian yang menguji kemampuan jamur indigenus Riau (Penicillium sp. PN6) dan legum akuatik (Neptunia oleracea) dalam meremediasi senyawa hidrokarbon dengan pengukuran total petroleum hidrokarbon (TPH) pada limbah oil sludge telah dilakukan. Pertumbuhan N. oleracea dilakukan pada media limbah oil sludge dengan konsentrasi 25, 50, 75, dan 100%. Selanjutnya dilakukan pemberian inokulum Penicillium sp. PN6 ke dalam media pertumbuhan. Hasil penelitian menunjukkan bahwa persentase pertumbuhan N. oleracea adalah 95,58; 85,87; 25,55; dan 0%, berturut-turut pada limbah oil sludge 25, 50, 75, dan 100%. Terjadi penurunan konsentrasi TPH awal pada semua perlakuan di akhir pengamatan. Penicillium sp. PN6 meningkatkan degradasi TPH dari limbah oil sludge dibandingkan dengan kombinasi Penicillium sp. PN6 dengan N. oleracea, tetapi kombinasi Penicillium sp.PN6 dengan N. oleracea meningkatkan persentase penurunan TPH dari limbah oil sludge dibandingkan dengan menggunakan Penicillium sp. PN6 saja. Penicillium sp. PN6 dan kombinasinya dengan N. oleracea sama-sama mampu menurunkan pH limbah oil sludge.Abstract Oil sludge contains the largest contaminant of petroleum hydrocarbon that needs treatment to prevent its pollution into the environment. A study examining the ability of a Riau indigenous fungi (Penicillium sp. PN6) and an aquatic legume (Neptunia oleracea) to remediate hydrocarbon compounds by determining total petroleum hydrocarbon (TPH) in oil sludge waste has been conducted. The growth of N. oleracea was performed in the oil sludge waste media by concentrations of 25, 50, 75, and 100%. The medium was inoculated with Penicillium sp. PN6. The results showed that the percentage of growth of N. oleracea were 95.58; 85.87; 25.55; and 0%, in the oil sludge waste of 25, 50, 75, and 100%, respectively. There was a decrease in initial TPH concentration at all treatments at the end of the observation. Penicillium sp. PN6 increased more TPH degradation of the oil sludge waste compared to a combination of Penicillium sp. PN6 and N.oleracea, but the combination increased the decrease percentage of TPH compared to those that used Penicillium sp. PN6 only. Penicillium sp. PN6 and its combination with N. oleracea are both able to decrease pH of the oil sludge waste. Permalink/DOI: http://dx.doi.org/10.15408/kauniyah.v11i1. 6350

BIODIVERSITAS DAN POTENSI JAMUR BASIDOMYCOTA DI KAWASAN KASEPUHAN CISUNGSANG, KABUPATEN LEBAK, BANTEN Ahmad Ni’matullah Al Ulya; Suroso Mukti Leksono; Rida Oktorida Khastini
Al-Kauniyah: Jurnal Biologi Vol 10, No 1 (2017): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Abstrak Biodiversitas jamur Basidiomycota di kawasan konservasi Taman Nasional Gunung Halimun Salak (TNGHS) yang terletak di Kabupaten Lebak, Provinsi Banten, belum pernah diteliti sebelumnya. Kawasan konservasi tersebut dihuni oleh masyarakat adat dari Kasepuhan Cisungsang, yang selama ini memanfaatkan jamur dalam kehidupan sehari-hari. Oleh karena itu, penelitian ini mengeks-plorasi keanekaragaman jamur Basidiomycota dan pemanfaatannya oleh masyarakat adat Kasepuhan Cisungsang. Penelitian dilaksanakan pada bulan Maret-Mei 2016. Sebanyak 34 spesies dari 21 marga, 16 keluarga, dan 5 bangsa dari jamur Basidiomycota berhasil ditemukan di daerah sawah, pekarangan, kebun, talun atau dudukan, dan hutan. Tujuh marga yang ditemukan diketahui dapat dimanfaatkan sebagai sumber makanan, yaitu supa ceuli (Auricularia sp.); supa amis (Marasmiellus sp.); supa beas (Coprinus sp.); supa tiram (Pleurotus sp.); supa jerami (Volvariella sp.); suung tunggal (Termitomyce sp.); dan supa kebo (Boletus sp.). Data ini menunjukkan tingginya biodiversitas jamur Basidiomycota di daerah masyarakat adat wilayah ini dan potensinya sebagai sumber makanan. Abstract Biodiversity of fungi Basidiomycota in the conservation area of Taman Nasional Gunung Halimun (TNNGHS) Salak in Lebak, Province Banten, has never been studied. This area is resided by indigenous people from Kasepuhan Cisungsang, which uses the fungi in their life. Therefore, this study was aimed at exploring the biodiversity of mushrooms (Basidiomycota) in Kasepuhan Cisungsang. The exploration was conducted from March to May 2016. A total number of 34 species which belong to 21 genera, 16 families, 5 orders were found in the rice field, yard, garden, and forest. About 7 genera are commonly consumed by the community. These include supa ceuli (Auricularia sp.); supa amis (Marasmiellus sp.); supa beas (Coprinus sp.); supa tiram (Pleurotus sp.); supa jerami (Volvariella sp.); suung tunggal (Termitomyce sp.); and supa kebo (Boletus sp.). This study showed a high diversity of mushroom in this area and their potential as a food source.

Prevalensi Mikrofilaria setelah Pengobatan Masal 4 Tahun di Wilayah Kampung Sawah, Kecamatan Ciputat, Tangerang Selatan Silvia F. Nasution; Evi Ekawati
Al-Kauniyah: Jurnal Biologi Vol 6, No 2 (2013): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Limphatic filariasis is a target of global diseases elimination promoted by WHO to accomplish by the year of 2020.Until 2008, 316 out of 471 districts/provinces were mapped epidemiologically as endemic areas of filariasis. South Tangerang is one of the district reported as an endemic area of filariasis, with Mf prevalence ranged 1 – 2.4% determined from 2002 to 2009. Some factors play important roles in elimination program of filariasis, such as an appropriate diagnostic and its evaluation, and also annual program of Mass Drug Administration (MDA). In Ciputat area, South Tangerang district, the MDA has been conducted annually since 2009, and evaluation program by finger blood sample was also conducted in 2002 and 2009. Finding result showed prevalence 1.6% of Mf and 8 individu were clinically diagnosed as elephantiasis. Although the MDA has been conducted every year, but the evaluation has not yet been performed after 2009. The Study aimed to evaluate the MDA effect against mikrofilaria rate (Mf) and antigen circulation in perifer blood of people living near by the elephantiasis individu in Kampung Sawah area, Ciputat, South Tangerang. Periferal blood and blood vessel were collected at night from 08.00–10.00 PM. Peripheral blood were directly swab on slide or object glass to overnight preserve for giemsa stain in laboratory. After night preservation, the slide were then identified by microscope to detect microfilaria. The blood vessel were sentrifuged to collect the serum and performed rapid diagnostic test antigen antifilaria IgG4. Finding result of the study determined 95% Mf negative and 72.5 % negative of antigen anti-filaria IgG4. These finding showed that more than 70% of subjects were negative to filarial and its antigen circulation in the blood.This also indicates successfull program of 4 years MDA in this study area to eliminate parasite of filariasis.

PERTUMBUHAN DAN KANDUNGAN POLIFENOL TANAMAN KATUK (Sauropus androgynus (L.) Merr) PADA MEDIA TANAM DENGAN PEMBERIAN ASAM HUMAT Elly Proklamasiningsih; Iman Budisantoso; Inayatul Maula
Al-Kauniyah: Jurnal Biologi Vol 12, No 1 (2019): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

AbstrakKatuk (Sauropus androgynus (L.) Merr) merupakan tanaman sayuran yang berkhasiat obat karena mengandung senyawa aktif diantaranya flavonoid, steroid, dan tanin yang digunakan sebagai antioksidan. Peningkatan pertumbuhan tanaman dapat dilakukan dengan meningkatkan produksi biomassa tanaman, yang diharapkan dapat meningkatkan kandungan senyawa aktif pada tanaman seperti polifenol. Penggunaan asam humat dapat meningkatkan kualitas media tanaman sehingga pertumbuhan tanaman dapat meningkat. Tujuan penelitian ini adalah untuk mengetahui pengaruh asam humat terhadap pertumbuhan dan kandungan senyawa polifenol tanaman katuk, serta menentukan konsentrasi asam humat yang efektif untuk meningkatkan pertumbuhan dan kandungan polifenol terhadap tanaman katuk. Penelitian ini dilakukan dengan metode eksperimental dengan rancangan acak lengkap yang terdiri dari 4 perlakuan asam humat pada media tanam dengan konsentrasi 0 g/kg; 4g/kg; 8g/kg dan 12 g/kg. Masing-masing perlakuan diulang sebanyak tiga kali ulangan. Parameter yang diamati dalam penelitian ini adalah pertumbuhan tanaman meliputi jumlah daun, berat basah dan berat kering serta kandungan polifenol. Data dianalisis dengan analisis variansi dengan taraf uji 5% dan 1% dan dilanjutkan dengan uji beda nyata terkecil pada level 5%. Pemberian asam humat dengan konsentrasi 6,23 g/kg pada media tanam dapat meningkatkan pertumbuhan tanaman dan kandungan polifenol tanaman katuk. .Kata kunci: Abstract Katuk (Sauropus androgynus (L.) Merr) is medicine efficacious vegetable because it contains active compounds such as flavonoid, steroid, and tannin. Those compounds were used as antioxidants. The growth of the plant increased the production of the biomass plant. It is expected that increasing biomass plant can increase active compound content in the plant like polyphenols. The use of humic acid can improve the quality of plant medium so the growth of the plant can increase as well. The purpose of this study was to find out the effect of humic acid to the growth and polyphenols content in katuk plant and to determine the concentration of acid humic that effective to increase the growth and polyphenol content to katuk plant. This research used an experimental method with a fully randomized design that consists of four humic acid treatments to plant medium with concentration of0 g/kg; 4g/kg; 8g/kg and 12 g/kg. Each treatment was repeated for three times. The parameters observed in this research were the leaf number, the wet weight, and the dry weight and the polyphenols content. The data were analyzed by analysis of variance with a 5% and 1% level of confidence , and continued to the least significant differences test at 5% level of confidence. The results showed that the addition of humic acid by 6.23 g/kg to the growing media increased the plant growth and polyphenols content in katuk plant.

PEMBENTUKAN TUNAS DARI BIJI MANGGIS (Garcinia mangostana L.) ASAL BENGKALIS DENGAN PENAMBAHAN BAP DAN MADU SECARA IN VITRO Mayta Novalida Isda; Siti Fatonah; Lia Novita Sari
Al-Kauniyah: Jurnal Biologi Vol 9, No 2 (2016): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

AbstrakGarcinia mangostana L. merupakan salah satu spesies dari famili Guttiferae kaya senyawa dari golongan xanthon. Manggis memiliki juvenil yang lama. Salah satu metode yang dapat memecahkan masalah dalam perbanyakan tanaman manggis dalam jumlah yang banyak, bisa sepanjang musim dan menghasilkan tanaman yang seragam yaitu kultur in vitro. Tujuan dari penelitian ini adalah untuk menentukan konsentrasi terbaik antara Benzil Amino Purin (BAP) dan madu terhadap induksi tunas dari eksplan biji manggis yang belah tiga melintang secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan perlakuan BAP (0;3 mg/L) dan madu (3;6 mg/L) pada media MS (Murashige-Skoog) dengan pengamatan 70 hari setelah tanam (hst). Hasil penelitian menunjukkan bahwa pemberian BAP maupun madu mampu meningkatkan pembentukan tunas. Pemberian BAP dan madu berpengaruh nyata terhadap persentase terbentuknya tunas, waktu muncul tunas, jumlah tunas dan panjang tunas. Jumlah tunas terbanyak terdapat pada perlakuan 3 mg/L BAP sebanyak 20 tunas per biji. Kombinasi perlakuan dengan penambahan 3 mg/L BAP + 3 ml/L madu menghasilkan persentase terbentuk tunas tertinggi (100%); waktu muncul tunas tercepat 12,75 hst; dan panjang tunas tertinggi 1,86 cm. Pada penelitian ini telah berhasil menginduksi tunas dari biji manggis yang dibelah tiga melintang asal Bengkalis dengan penambahan BAP dan madu.AbstractGarcinia mangostana L. is one of the species within the family Guttiferae that rich in compounds from the class of xanthon. Mangosteen has time juvenile problems. One method that can solve the problem in the mangosteen plant propagation which can be produced in a large number in all season and uniform is in vitro culture. The purpose of this study was to determine the best concentration of BAP and honey to induce the formation of shoot from explants of mangosteen seeds split transversely into three pieces by in vitro method. This study used a Completely Randomized Design (CRD) with a treatment of BAP (0;3 mg/L) and honey (3;6 mg/L) on MS medium with 70 days of observation. The results showed that the BAP and honey can improve the formation of shoots. The addition of BAP and honey significantly affected the percentage of the formed shoots, the period of shoots appear, the number of shoots and shoot length. The treatment of 3 mg/L BAP resulted in the most shoots formed as many as 20 shoots per seed. The combined treatment 3 mg/L BAP + 3 ml/L of honey produced 100% of the formed shoots; fastest period of shoots appear (12.75 DAS); and the longest shoot (1.86 cm). Therefore, the addition of BAP and honey to the three transversely-split mangosteen seeds from Bengkalis origin successfully induced the formation of the shoot.

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Reviewer

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Contact Name
Dr. Nani Radiastuti

Contact Email
n_radiastuti@yahoo.com

Phone
-

Journal Mail Official
alkauniyah@uinjkt.ac.id

Editorial Address
-

Location
Kota tangerang selatan,

Banten

INDONESIA

Abstract

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Dr. Nani Radiastuti

Contact Email n_radiastuti@yahoo.com

Phone -

Journal Mail Official alkauniyah@uinjkt.ac.id

Editorial Address -

Location Kota tangerang selatan, Banten INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Dr. Nani Radiastuti

Contact Email
n_radiastuti@yahoo.com

Phone
-

Journal Mail Official
alkauniyah@uinjkt.ac.id

Editorial Address
-

Location
Kota tangerang selatan,

Banten

INDONESIA