cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota adm. jakarta selatan,
Dki jakarta
INDONESIA
Jurnal AgroBiogen
Published by Kementerian Pertanian
ISSN : 19071094     EISSN : 25491547     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
Arjuna Subject : -
Articles 252 Documents
 1   2   3   4   5 
Identifikasi Marka Polimorfik untuk Pemuliaan Padi Toleran Defisiensi Fosfor Joko Prasetiyono; Hajrial Aswidinoor; Sugiono Moeljopawiro; Didy Sopandie; Masdiar Bustamam
Jurnal AgroBiogen Vol 4, No 2 (2008): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v4n2.2008.p51-58

Abstract

Information on polymorphismsamong rice parents are very important in ricebreeding for tolerance to phosphorus defficiency. A studywas conducted at the Molecular Biology Laboratory,Indonesian Center Agricultural Biotechnology and GeneticResources (ICABIOGRAD) from October 2006 to July 2007 toidentify polymorphism markers from 6 rice genotypes. Therice genotypes, i.e., Dodokan, Situ Bagendit, Batur, Kasalath,NIL-C443, dan K36-5-1-1 were analyzed for polymorphismsusing 496 SSR markers, which cover the rice genomes.Seven of the 496 markers were used as foreground andrecombinant selection markers, and the rests (489 markers)were used as background selection markers. PCR amplificationswere separated on a 5% polyacrylamide gel andcolored by the silver staining method. Three different markersamong the seven foreground and recombinant selectionmarkers were selected from each crossing, which aretightly linked with Pup1 gene and have a distance less than 5cM. These markers are Dodokan vs Kasalath (RM277, SSR3,RM519), Dodokan vs NIL-C443 (RM277, SSR3, RM519),Dodokan vs K36-5-1-1 (RM277, SSR3, RM519), Situ Bagenditvs Kasalath (RM28102, SSR3, RM519), Situ Bagendit vs NILC443(RM28102, SSR3, RM519), Situ Bagendit vs K36-5-1-1(RM511, SSR3, RM519), Batur vs Kasalath (RM277, RM1261,RM519), Batur vs NIL-C443 (RM277, RM1261, RM519), andBatur vs K36-5-1-1 (RM28102, SSR3). Variations in backgroundselection primers were found in each chromosomeand in each parent combinations. Primers on chromosome4, 5, and 12 showed the lowest polymorphisms; moreprimers are needed for these chromosomes.
Transformasi Genetik Tembakau dengan Gen Cold Shock Protein melalui Perantara Agrobacterium tumefaciens Seagames Waluyo; Sustiprijatno Sustiprijatno; Suharsono Suharsono
Jurnal AgroBiogen Vol 9, No 2 (2013): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v9n2.2013.p58-65

Abstract

Coldshock protein (Csp) essential for organisms to survive inabiotic stress condition. CspB gene has been fused toubiquitin promoter in the T-DNA region of pCambia 1300int,and introduced into Agrobacterium tumefaciens LBA4404.This research had an objective to transform geneticallyNicotiana tabacum cv. Samsun by CspB gene under thecontrol of Ubiquitin promoter and NOS terminator mediatedby A. tumefaciens. Leaf discs were co-cultivated with A.tumefaciens LBA 4404. Based on the number of hygromycinresistantcalli, the efficiency of transformation was 57.5%. Inthe selective medium containing 50 μg/l hygromycin, theefficiency of regeneration of transgenic shoots was 82.6%.Based on PCR analysis using primers corresponding toubiquitin promoter and CspB gene, 18 putative tobaccotransgenic containing CspB gene under the control ofubiquitin promoter.
Construction and Expression of Pet Operon using Shuttle Vector for Mesophilic and Thermophilic Bacteria Eny Ida Riyanti; Peter L. Rogers
Jurnal AgroBiogen Vol 5, No 1 (2009): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v5n1.2009.p7-15

Abstract

Keuntungan fermentasi etanol pada suhu tinggi mendorong penelitian perakitan bakteri termofilik etalogenik. Selain itu, kemampuan bakteri termofilik dalam penggunaan gula pentosa hasil degradasi biomasa memberi peluang untuk menekan biaya produksi bioetanol. Tujuan dari penelitian ini adalah untuk mengkonstruksi pet (production of ethanol) operon dengan menggunakan shuttle vector pMK18 dan melihat ekspresinya dalam bakteri mesofilik dan termofilik. Konstruksi dan ekspresi pet operon dengan menggunakan adhT dari bakteri termofilik dan pdc dari bakteri mesofilik, dan penggunaan mesofilik-termofilik shuttle vector sebagai backbone-nya baru pertama kali dilaporkan. Pet operon adalah suatu susunan gen penyandi produksi etanol yang terdiri dari gen pdc (pyruvate decarboxylase) dan adh (alcohol dehydrogenase). Konstruksi pet operon menggunakan gen adhT dari bakteri termofilik Geobacillus thermoglucosidasius M10EXG dan pdc (pyruvate dehydrogenase) dari bakteri mesofilik Zymomonas mobilis ZM4 telah dilakukan dengan menggunakan mesofilik-termofilik shuttle vector pMK18. Ekspresi pet operon pada bakteri mesofilik Eschericia coli dapat memproduksi 0,3 g/l etanol dengan aktivitas adhT sekitar 0,02 U/mg protein dan aktivitas pdc sekitar 0,004 U/mg protein. Perlu dilakukan penelitian lanjutan untuk perbaikan konstruksi pet operon untuk sistemtermofik pada Thermus thermophilus HB27, karena konstruksi yang didapat belum optimum untuk sistem termofilik ini. Hasil ini diharapkan akan mengawali pengembanganteknik manipulasi genetik pada bakteri termofilik yang masih sangat terbatas, khususnya pengembangan teknik manipulasi termofilik etanologenik. Kata kunci: Etanol, bakteri termofilik, bakteri mesofilik, pet operon, ekspresi gen. 
Kultur In Vitro Endosperma, Protokol yang Efisien untuk Mendapatkan Tanaman Triploid secara Langsung Lazarus Agus Sukamto
Jurnal AgroBiogen Vol 6, No 2 (2010): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v6n2.2010.p107-112

Abstract

In Vitro Culture of Endosperm: An efficient protocol topropagate triploid plants directly. L. Agus Sukamto.Triploid plants are very vigorous and beneficial since theygenerally produce seedless fruits, bigger flowers, and producemore volume of wood than the diploid counterparts.The triploid plants can be produced by crossing diploid andtetraploid plants, but this method is cumbersome and takesa long time. In vitro culture of endosperm is an alternativemethod to produce triploid plants directly. The success ofendosperm culture is dependent on many factors, such asmaturity of endosperm, presence of the zygotic embryo, culturemedium, growth regulators, browning, culture period,an plant species. Generally, a mature endosperm needs aninitial association with an embryo to induce cell divisions,while proliferation of an immature endosperms is notdependent on the embryo. Endosperm of most parasiticangiosperms shows direct organogenesis without callusformation. Plants produced from endosperm culture aregenerally triploid, although some plants possess differentploidy levels.
Perbanyakan Tanaman Jambu Mete (Anacardium occidentale L.) melalui Jalur Organogenesis Rossa Yunita; Ika Mariska; Christiani Tumilisar
Jurnal AgroBiogen Vol 8, No 3 (2012): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n3.2012.p113-119

Abstract

Vegetativepropagation through in vitro culture has been carried out asa technology that has the potential for obtaining seedling insignificant amounts and relatively faster. This activity can bedone through the multiplication of adventitious shoots andlateral shoots (organogenesis). The goal of this research wasto find the method of cashew micropropagation throughorganogenesis. This study consisted of 4 main activities.They were shoot induction, shoot multiplication, shootelongation, and root induction. The results showed the bestmedium composition for shoot induction was MS + BA 0.7mg/l. The suitable media for shoots multliplication was MS +thidiazuron 0.5 mg/l + zeatin 1 mg/l and for shootselongation was MS + GA 1 mg/l + zeatin + 3 mg/l. The bestmethods for root induction was by submerging in vitroshoots in a solution of IAA 100 mg/l.
Induksi Kalus dan Regenerasi Beberapa Genotipe Gandum (Triticum aestivum L.) secara In Vitro Atmitri Sisharmini; Aniversari Apriana; Sustiprijatno Sustiprijatno
Jurnal AgroBiogen Vol 6, No 2 (2010): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v6n2.2010.p57-62

Abstract

Callus Induction and In Vitro Plant Regeneration ofWheat Genotypes (Triticum aestivum L.). AtmitriSisharmini, Aniversari Apriana, and Sustiprijatno. Developmentof a reliable in vitro plant regeneration procedure forwheat is a prerequisite for its improvement by genetic transformation.The purpose of this study was to obtain methodsof callus induction and regeneration of wheat genotypes.This experiment was conducted at ICABIOGRAD. Immatureembryos from four wheat genotypes, ie Perdix, Naxos Wew,Combi and Fasan were used to induce callus formation andregeneration rate of callus. For the preparation of callusinduction medium, MS-L7 basal medium was supplementedwith combination of growth regulators 2,4 dichlorophenoxyacetic acid (2,4-D) and 4-amino-3,5,6-trichloropicolinic acid(picloram). While, plant regeneration medium was preparedusing MS basal medium supplemented with combination ofthree growth regulators i.e. IAA, BAP and kinetin. The resultsshowed that genotype, in vitro culture medium and growthregulators played a dominant role in callus induction andplantlet regeneration. All the 4 genotypes responded positivelyto callus induction, however, variability was observednot only among the genotypes but also within callusinduction medium used. The best induction medium wasthe MS-L7 basal medium supplemented with combination ofphytohormon 4 mg/l 2,4-D + 2 mg/l picloram (GIK-3) whichshowed 100% callus induction frequency. Whereas, the bestregeneration medium was shown by MS basal medium withcombination of phytohormon 1.5 mg/l BAP dan 0.5 mg/lkinetin (RG3). Regarding plant regeneration, Perdix was themost responsive genotype to be regenerated with regenerationfrequency of 57.33%. The successfully acclimatizedplanlets in greenhouse were obtained from Perdix andNaxos Wew genotypes. These results will potentially facilitategenetic transformation research of wheat in Indonesia.
Keragaman Genetik Kultivar Padi Beras Hitam Lokal Berdasarkan Penanda Mikrosatelit Kristamtini Kristamtini; Taryono Taryono; Panjisakti Basunanda; Rudi H. Murti
Jurnal AgroBiogen Vol 10, No 2 (2014): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v10n2.2014.p69-76

Abstract

Indonesia has diverseaccessions of local black rice, which are important sourcesof germplasm. However, some of the local black ricecultivars have different names, leading a need to beidentified to determine their genetic diversity usingmolecular marker. This study aimed to identify geneticdiversity of eleven cultivars of local black rice, collection ofthe Assessment Institute for Agricultural Technology,Yogyakarta and compared them with two white ricevarieties using four microsatellite markers. Detection ofmicrosatellite alleles polymorphism was carried out byvisualization of PCR amplicons by electrophoresis onagarose gel. To estimate their genetic diversity, phylogenetictree and principal coordinate analysis were performed usingbinary data of SSR alleles. The results revealed that totalmarkers enabled to differentiate black rice cultivars asreflected by high value of polymorphic information content(PIC) mean (0.695). This value was consistent with the highgenetic diversity of black rice (genetic diversity index, h =0.283) in comparison with white rice cultivars (h = 0.020).The phylogenetic and main coordinate analyses suggestedthat black rice cultivars genetically differed from white rice.The local black rice cultivars were preferentially groupedbased on their genetic those were distributed in threecoordinates and did not represent their local geographicorigin. Genetic diversity analysis in this study will be usefulas an initial basis for proper identification and selection forappropriate parents to assist breeding program of black ricein Indonesia.
Construction and Transformation of HVA1 Gene Expression Vector into Indonesian Elite Rice Varieties Sri Koerniati; Hani Widhianata
Jurnal AgroBiogen Vol 8, No 2 (2012): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n2.2012.p54-61

Abstract

of drought in rice. HVA1 is one of the Late EmbryogenesisAbundant (LEA) protein group that plays a role on cellprotection during stresses. A study was done with anobjective to construct a plasmid vector expressing HVA1 andto transform it into Indonesian elite rice varieties. Materialsused in the study were plasmid pBY520 (source of HVA1;intermediate plasmid pRP9; plasmid pAY560326(backbone); restriction enzymes BamHI, HindIII, XhoI, andSpeI; T4 DNA ligase, and gel DNA extraction kit. Methodsused were standard procedure for plasmid vectorconstruction and molecular biology. Step I: the pBY520 andpRP9 were cut with BamHI and HindIII, and electrophoratedwith 1% agarose gel. DNA fragments of HVA1 and pRP9 werepurified, ligated with T4 DNA ligase, and transformed intoEscherichia coli DH5-α by heat shock. E. coli were grownonto solid medium (+ kanamycin 100 mg/l). A new plasmidDNA was isolated from single colony culture of the bacteria,confirmed, and named pRP9_HVA1. Step II: DNA ofpRP9_HVA1 and pAY560326 were cut with XhoI dan SpeIenzymes, purified, and ligated. The next procedure wassimilar to step I, and the resulted plasmid was confirmed byPCR and digestion with XhoI dan SpeI enzymes, and namedpAY_HVA1. Step III: pAY_HVA1 was first transformed intoAgrobacterium EHA-105 and then into rive varieties Ciherangand Inpari 6 using the early infection of scutellumtransformation method. Nine transgenic rice lines thatpositively contain HVA1 were obtained.
Pengaruh Hormon Asam Indol Asetat yang Dihasilkan Azospirillum sp. terhadap Perkembangan Akar Padi Puji Lestari; Dwi N. Susilowati; Eny I. Riyanti
Jurnal AgroBiogen Vol 3, No 2 (2007): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v3n2.2007.p66-72

Abstract

Free-living bacteria of thegenus Azospirillum live in close association with rice roots.This bacteria produced indole acetic acid (IAA), a plantgrowth hormon, to the environment. IAA was isolated fromcultures of Azospirillum strains and investigated for its effecton root development and plant height of rice variety IR64 invitro. Rice cultures of variety IR64 were grown in vitro andinoculated with cultures of Azospirilllum. Production of IAAby the bacterium during its growth period in rice culture mediumcontaining different levels of nitrogen was observed.Results of the experiment showed that strains AzospirillumAz15 and Az44 had a high ability to produce IAA, i.e., 57.93μg/ml at 12 days after incubation (DAI) and 40.42 μg/ml at 7DAI, respectively. The IAA production pattern of AzospirillumAz15 and Az44 in the liquid medium were fluctuativeuntil the end of the incubation period, while that of the strainAz7 was linier. Strain Az7 gave a better effect on the rootdevelopment and plant height than strains Az15 and Az44.Treatment combination of strain Az7 and 100% nitrogen gavehighest root development. High level of nitrogen increasedIAA content in the uninoculated culture, while low IAAcontent on the inoculated one. Inoculation the culture withstrain Az7 together with 50% nitrogen application resulted inthe IAA content, root dry weight, root length, fiber root number,and plant height as high as those on cultures containing100% nitrogen (1 mM NH4NO3) without inoculation. Inoculationof rice culture with Azospirillum is expected to reducenitrogen application on rice IR64 by the IAA production asindicated by significant changes in the root growth anddevelopment. A higher concentrations of IAA tend to givebetter effects on the root growth and development of riceIR64.
Resistance and Phenotypic Character of Chili M2 Mutant Lines Against Chilli Veinal Mottle Virus Ifa Manzila; Neni Gunaeni; Yenni Kusandriani; Tri P. Priyatno
Jurnal AgroBiogen Vol 11, No 2 (2015): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v11n2.2015.p73-80

Abstract

Chilli veinal mottle virus infection (ChiVMV) could reduce the quality and 60–100% of yield losses of chili. Among the chilivarieties released, no one has been resistant to ChiVMV, mainly due to a high variation of ChiVMV strains and not well mapped.Therefore, finding a new source of ChiVMV resistant genes is pivotal role in order to assembly new varieties. Approach throughin vitro mutation induction using mutagen ethyl methane sulfonate (EMS) is one of the efforts to increase genetic diversity.Previous studies has successfully acquired 800 M2 lines through callus induction of Gelora variety with EMS. This study aimed toobtain M2 lines resistant to ChiVMV and having a good agronomical characters. A total of 800 chili M2 lines that derived from chiliM2 mutations using mutagen EMS has been tested in greenhouse to ChiVMV resistance and studied character phenotype. Theresults showed that of the 800 lines, there were 28 strains obtained showed a response tolerant and resistant to ChiVMV. Eightmutant lines of which have good agronomic characters. The mutant lines are M2.100, M2.108, M2.200, M2. 122, M2.238, M2.353,M2.420, and M2.517. Eight lines will be selected and further observed to obtain chili promising lines that are resistant to ChiVMVand high yielding.

Page 3 of 26 | Total Record : 252

 1   2   3   4   5 

Filter by Year

2005 2021


Reset
Filter By Issues
All Issue Vol 17, No 2 (2021): DESEMBER Vol 17, No 1 (2021): JUNE Vol 16, No 2 (2020): December Vol 16, No 1 (2020): June Vol 15, No 2 (2019): December Vol 15, No 1 (2019): June Vol 14, No 2 (2018): December Vol 14, No 1 (2018): June Vol 14, No 1 (2018): June Vol 13, No 2 (2017): Desember Vol 13, No 1 (2017): Juni Vol 12, No 2 (2016): Desember Vol 12, No 1 (2016): Juni Vol 11, No 3 (2015): Desember Vol 11, No 2 (2015): Agustus Vol 11, No 1 (2015): April Vol 10, No 3 (2014): Desember Vol 10, No 2 (2014): Agustus Vol 10, No 1 (2014): April Vol 9, No 3 (2013): Desember Vol 9, No 2 (2013): Agustus Vol 9, No 1 (2013): April Vol 8, No 3 (2012): Desember Vol 8, No 2 (2012): Agustus Vol 8, No 1 (2012): April Vol 8, No 1 (2012): April Vol 7, No 2 (2011): Oktober Vol 7, No 1 (2011): April Vol 7, No 1 (2011): Jurnal AgroBiogen Vol 6, No 2 (2010): Oktober Vol 6, No 1 (2010): April Vol 5, No 2 (2009): Oktober Vol 5, No 1 (2009): April Vol 4, No 2 (2008): Oktober Vol 4, No 1 (2008): April Vol 3, No 2 (2007): Oktober Vol 3, No 1 (2007): April Vol 2, No 2 (2006): Oktober Vol 2, No 1 (2006): April Vol 1, No 2 (2005): Oktober Vol 1, No 1 (2005): April More Issue