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INDONESIA
Jurnal AgroBiogen
Published by Kementerian Pertanian
ISSN : 19071094     EISSN : 25491547     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
Arjuna Subject : -
Articles 252 Documents
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QTL Study to Reveal Soybean Response on Abiotic and Biotic Stresses Puji Lestari; Sutrisno Sutrisno; I Made Tasma
Jurnal AgroBiogen Vol 10, No 3 (2014): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v10n3.2014.p109-114

Abstract

As an important grain legume, the improved soybean(Glycine max [L.] Merr.) adaptive to environmental changesis a valuable genetic resource. Strategy to minimize theimpact of climate effects should be underlined on soybeanproduction encompassing advanced genomics and wellpredicted future climate. Crops including soybean respondto climate change in the aspect of abiotic and bioticenvironmental factors. To predict soybean response toabiotic and biotic stresses, current progress of quantitativetrait loci (QTL) for abiotic and biotic stresses and floweringand related genomic resources could be accessed atSoyBase (http://www.soybase.org) and Phytozome(http://www.phytozome.net). As the involvement of abioticand biotic stresses modulating flowering in soybean, geneslinked to QTL for abiotic/biotic stress and flowering/maturitywere also potential for resisting the environmental changes.By mapping QTLs for flowering using one population indifferent locations (Korea and China) with distinctivelongitude, latitude, and altitude, syntenic correlationbetween these two QTLs on soybean chromosomes 6 and13 indicates the environmental specific role of syntenicregions. The information on QTL and related candidategenes may assist marker-assisted breeding and enactsoybean as a model of adaptive legume crop under abiotic/biotic stress.
Regenerasi Pepaya melalui Kultur In Vitro Diani Damayanti; Sudarsono Sudarsono; Ika Mariska; M. Herman
Jurnal AgroBiogen Vol 3, No 2 (2007): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v3n2.2007.p49-54

Abstract

A study was conducted in the Indonesian Center forAgricultural Biotechnology and Genetic Resources Researchand Development to optimize papaya regeneration systemsthrough in vitro culture. Four steps were done, i.e., callusinduction, callus regeneration, root formation, and acclimatization.Explant materials used were immature embryos ofpapaya cv. Burung. Immature papaya embryos were culturedon different media. The best medium for embryogeniccallus development was ½ MS + 10 mg/l 2.4-D + 60% sucrose+ 143 mg/l adenine sulphate + 50 mg/l myo inositol +400 mg/l glutamine, while that for callus embryo regenerationwas MS + 0.5 mg/l GA3 + 0.1 mg/l kinetin + Morel andWetmore Vitamin. Using this medium, the average of shootformation was three shoots per explant of embriogeniccallus, and the percentage of regenerated callus was 80%.The color of shoot derived from this treatment was green.Eighty percent of plants formed a complete root developmentusing ½ MS + 0.5 mg/l paclobutrazol media. Media hullof rice and compost was the best medium for papaya plantacclimatization. The percentage of survival on that acclimatizationstep was 65%.
Analisis Gen Selubung Protein Chilli Veinal Mottle Potyvirus dari Beberapa Daerah di Indonesia Ifa Manzila; Sri H. Hidayat; Ika Mariska; Sriani Sujiprihati
Jurnal AgroBiogen Vol 8, No 1 (2012): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n1.2012.p27-37

Abstract

Variation on symptoms and virulence wasobserved on different isolates of ChiVMV collected fromWest Java, Central Java, East Java, South Kalimantan, WestSumatera and Central Aceh. Research was conducted tostudy genetic variation of six ChiVMV isolates based onsequence analysis of coat protein (CP) gene and aminoacid. Sequence analysis of CP gene showed 87% to 99%homology among the six isolates with level of variationranging from 0.02% to 1.48%. Sequence analysis of aminoacid derived from CP gene showed 85% to 99% homology.Further analysis on amino acid motives of CP gene indicatedmutation of octapeptide motif, i.eLSGQVQPQSRQSEMETEVPQVR on ChiVMV CKB andRMETFGLDGRVGTQEEDTERHT on other ChiVMV isolates.Other differences was observed on amino acid number 61and 84 i.e. deletion of MET and mutation of GG to KV onChiVMV BL and KR. Phyllogenetic analysis based onnucleotide and amino acid sequence showed that sixisolates of ChiVMV can be differentiated into three groups.ChiVMV KR and BL were in the same group with ChiVMVPataruman (GeneBank No. access DQ854961), ChiVMV CKBwas in the same group with ChiVMV Cikabayan 2(GeneBank No. access DQ854960), and ChiVMV TD, ChiVMVNI and GB ChiVMV were in the same group with ChiVMVTaiwan (GeneBank No. access DQ854948). Analysis of CPgene confirmed the occurrence of genetic variation amongChiVMV isolates although symptom variation is weak.
Perbanyakan Nematoda Patogenik Serangga (Rhabditida: Steinernema dan Heterorhabditis) pada Media In Vitro Cair Statik Chaerani Chaerani; M. Ace Suhendar; J. Harjosidarmop
Jurnal AgroBiogen Vol 8, No 1 (2012): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n1.2012.p19-26

Abstract

Entomopathogenic nematodes belongingto genera Steinernema and Heterorhabditis are potentiallymost effective and safe biological control agents for insectpests, especially for soil dwelling insects and those living incryptic habitats. Field application of the nematodes is stillhampered by supply of large number of infective juvenile(IJ) nematodes. Five published in vitro media along with itstwo modifications were tested for mass propagations of twoindigenous nematodes (H. indicus PLR2 and SteinernemaT96) and one commercial strain (S. carpocapsae #25).Varying levels of IJ yields were observed across thereplications and experiments. Medium F that contained 1.0%yeast extract, 2.5% egg yolk, and 4.0% soy oil yielded thehighest IJ numbers of H. indicus PLR2 (1.5×105 IJ ml-1) andof S. carpocapsae #25 (2.9×105 IJ ml-1), whereas the widelyused medium B, which is based on homogenized chickenoffal (40%), yielded the highest number of Steinernema T96(5.8×104 IJ ml-1). The IJ’s quality, as measured by theirmorphometrics and pathogenicities, were generallyimpaired, indicating the lack of essential nutrient(s) in themedia. Optimization of the propagation media is thereforestill needed to increase IJ’s quantity and quality to achievethe required standard for commercial scale of artificialpropagation.
Pemanfaatan Teknik Kultur In Vitro untuk Konservasi Plasma Nutfah Ubi-ubian Nurwita Dewi; Iswari S. Dewi; Ika Roostika
Jurnal AgroBiogen Vol 10, No 1 (2014): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v10n1.2014.p34-44

Abstract

Except for potato, sweet potato, taro, yam, andcassava, most of tuber crops are considered as underutilizedcrops. However, tuber crops are potential as alternativecarbohydrate sources, so they can be used as food reservesto face global climate change that affects food security incertain area throughout the world, including Indonesia.Having high diversity in tuber crops germplasm, Indonesiamust be able to conserve those germplasm to ensure theiravailability in the future. In the future, without ignoring allthe probable constraints, the prospect in utilization of in vitroculture technique will be higher for improvement ofconservation and management of genetic resources in theform of active and base collections. In this paper, strategy indeveloping in vitro collection of tuber crops germplasm, i.e.slow growth technique for medium term storage andcryopreservation technique for long term storage, isdiscussed including how to analyze genetic stability of thecollections. Several national and international researchcenters dealing with research and development of in vitroconservation technique are presented.
Identitas dan Keragaman Genetik Begomovirus yang Berasosiasi dengan Penyakit Keriting pada Tomat Berdasarkan Teknik Polymerase Chain Reaction (PCR)- Restriction Fragment Length Polymorphism (RFLP) Tri Joko Santoso; Sri H. Hidayat; M. Herman; H. Aswidinnoor; Sudarsono Sudarsono
Jurnal AgroBiogen Vol 4, No 1 (2008): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v4n1.2008.p9-17

Abstract

Begomoviruses, members of the Geminivirus,are considered as emerging plant viruses. This was due tothe increasing incidences and severities of the diseases in anumber of economically important crops, including tomato.Genetic diversities of the Begomovirus isolates infectingtomato (Lycopersicon esculentum) of several areas in Indonesiawere analyzed by using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP)technique. A 1500 base pairs of PCR fragments amplified byusing degenerate primers for Begomovirus was digestedusing four restriction enzymes, i.e., DraI, EcoRI, RsaI, andPstI. The pattern of RE digested fragments of 8 Begomovirusisolates and the predicted RFLP fragments of the Begomovirusisolates in the GeneBank database were used to determinethe genetic identities and diversities among the isolates.Positive results of the PCR amplifications proved thatdiseased tomato plant samples collected from 8 locations inJava and Sumatra were infected with at least one Begomovirusisolate. The PCR amplification products, which weredigested using the four restriction enzymes indicated thepresence of polimorfisms among the DNA fragments of theBegomovirus isolates. Identifications of the Begomovirusindicated that the Brastagi, Bogor, Sragen, Ketep, and Boyolaliisolates were Tomato Leaf Curl Virus (ToLCV); theisolates from Malang and Blitar isolates were AgeratumYellow Vein Virus (AYVV), while one isolate from Kaliurangwas Tomato Yellow Leaf Curl Virus (TYLCV). Results of thephylogenetic analysis of the 8 Begomovirus isolates basedon Begomoviruses from the DNA database indicated thatthey belonged to three different groups.
Uji Pendahuluan Genotipe-genotipe Kedelai Hasil Seleksi In Vitro terhadap Cekaman Aluminium dan pH Rendah Arief Vivi Noviati; Sri Hutami; Ika Mariska; Endang Sjamsudin
Jurnal AgroBiogen Vol 1, No 2 (2005): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v1n2.2005.p73-75

Abstract

Aluminum toxicity is a major constraint to soybean production in acid soils. Since variabilities on Al tolerance in plants are very limited, mutation breeding, and in vitro selection were used to increase the variability. Three soyben genotypes were produced from cultivars Wilis and Sindoro that have been gamma irradiated and selected in vitro for their tolerance to Al on Al and low pH media. These genotypes and their original cultivars were then planted in a greenhouse in an acid soil on May 2001. The results showed that the plant performances were varied, some were shorter and more compact than the original. Based on the yield components, a number of plants from the genotypes showed higher than those of the control cultivars. These plants were considered more tolerant to Al than the original cultivars.
Development of SSR Marker Set to Identify Fourty Two Indonesian Soybean Varieties Andari Risliawati; Eny I. Riyanti; Puji Lestari; Dwinita W. Utami; Tiur S. Silitonga
Jurnal AgroBiogen Vol 11, No 2 (2015): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v11n2.2015.p49-58

Abstract

Profile of molecular marker can be used for variety identification, genetic purity monitoring of germplasm and additionalrequirement in proposing intellectual property protection. DNA fingerprinting of soybean had been applied at the ICABIOGRADIAARDsince 2004 using simple sequence repeat (SSR) markers which were run automatically by CEQ 8000 Genetic Analyzerplatform based on capillary electrophoresis system. This method had produced unique DNA fingerprints of the varieties tested,but the marker set to efficiently identify the varieties had not yet been developed. This study aimed to develop a set of SSRmarkers as a tool to identify the Indonesian soybean varieties. Fourty two soybean varieties were analyzed using 14 random SSRmarkersA total of 168 alleles that were obtained from the polymorphism analysis. The average of polymorphic informationcontent (PIC) value observed was 0.7337 per SSR locus. Based on marker reproducibility rate, PIC value, number of rare alleles,frequency of dominant alleles, and percentage of SSR fragment detected by genetic analyzer, we identified five SSR markers i.e.Satt414, Satt147, Satt308, Satt009, and Satt516 as a SSR marker set to be used for soybean variety identification purposes. Thismarker set was used to develop the identity (ID) of the 42 Indonesian soybean varieties.
Segregation Analysis of SSR, SNP, and AFLP Markers in F2 Population of Solanum lycopersicum × S. arcanum (Analisis Segregasi Marka SSR, SNP, dan AFLP pada Populasi F2 Persilangan Solanum lycopersicum × S. arcanum) Chaerani Chaerani; R. E. Voorrips
Jurnal AgroBiogen Vol 11, No 1 (2015): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v11n1.2015.p1-6

Abstract

Distorted marker segregation is a common phenomenon in interspecific cross of various crops. Previous mapping study of earlyblight fungus (Alternaria solani) resistance loci showed 52% marker distortion in the genetic linkage map of 176 F2 progeniesderived from Solanum lycopersicum cv. Solentos × S. arcanum LA2157. The objectives of this study were to analyze in detail themarker segregation in the map and to determine the cause of segregation distortion by calculating the allele and genotypefrequencies of each marker. Out of 371 mapped markers, 192 markers deviated from the expected Mendelian ratio of 1 : 2 : 1.Distorted markers occurred in all chromosomes, ranging from 1% to 92%. Surplus of S. arcanum homozygotes contributed mostto the skewness (40%), followed by heterozygotes (18%), and S. lycopersicum homozygotes (5%). The allele frequencies of 152markers deviated from the expected allele homogeneity frequency, indicating that their segregation might be affected bygamethophytic selection. Sixty-one markers deviated from the expected F2 genotype frequency distribution, indicating that theirsegregation might be influenced by zygotic selection. Thirty-seven of the distorted markers showed deviation from expectedfrequencies of allele homogeneity and F2 genotype frequency distribution. Distorted markers can be retained in linkage analysissince chromosomal regions containing distorted markers showed linkage with early blight fungus resistance loci. Furtheridentification of the mechanism contributing segregation distortion requires detailed and extensive mapping studies.
Pengembangan Set Multipleks Penanda DNA Mikrosatelit untuk Analisis Variasi Genetik Padi dan Kedelai Chaerani Chaerani; Nurul Hidayatun; Dwinita W. Utami
Jurnal AgroBiogen Vol 5, No 2 (2009): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v5n2.2009.p57-64

Abstract

Detection of multiplex microsatellite markers in asingle capillary array on a laser detection system is traditionallyconducted with specific primers that are labelled withfluorescent dyes. An alternative method using fluorescentlabels that are appended to 5’ end of universal primer M13instead of to the specific primers offers flexibility indesignning multiplex panels and a less expensive method.Allele size range of microsatellite loci that can be grouped inmultiplex panels can be accurately estimated by pooling andanalyzing DNA samples from several genotypes simultaneously.This paper describes the procedure in developmentof microsatellite multiplex panels using M13 fluorescentlylabelledand estimation of allele size range based on pooledDNA strategies. Two multiplex panels of PCR amplificationproducts for rice consisting of 15 loci and three panels forsoybean consisting of 10 loci have been designed. Thepanels have been applied to 50 accessions of rice and soybeanwith fairly good results. Further characterization ofallele size range, however, is required prior to the applicationof these panels to diverse genotypes. The proceduredescribed here should be applicable in the development ofmultiplex panels of other species.

Page 5 of 26 | Total Record : 252

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