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Biotropika
Published by Universitas Brawijaya
ISSN : 23027282     EISSN : 25498703     DOI : 10.21776/ub.biotropika.
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Biotropika: Journal of Tropical Biology invites research articles, short communication, and reviews describing new findings/phenomena of biological sciences in tropical regions, specifically in the following subjects, but not limited to biotechnology, biodiversity, microbiology, botany, zoology, biosystematics, ecology, and environmental sciences.
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Articles 554 Documents
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The utilization of Noni Fruit (Morinda citrifolia L) As the Ice Cream ingredient Anita Juhari; Rodliyati Azrianingsih; Amin Setyo Leksono
Biotropika: Journal of Tropical Biology Vol 5, No 3 (2017)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2017.005.03.3

Abstract

Abstract The objectives of this research were to determine of the society perception on the use of noni fruit and ice cream, technique to produce ice cream on processed products of noni, to know the content of the nutritional value of noni ice cream, as well as society prospects and perception toward noni ice cream products. The method of this research used a questionnaire survey involving semi structure direct interviews, marketing and beneficial calculation of noni ice cream products. The data were presented in graphical form followed by feasibility analysis. After the survey, 81% of respondents liked and interested in consuming ice cream noni products. Noni fruit mixture was added into the ice cream processed analyses showed that noni ice cream product healthier and have a high nutrient. Fat content of noni ice cream at 6.54 % and lower than the SII standard of 8 %, which indicated that the noni ice cream was low fat. Noni ice cream contain 36,89 mg/100g of vitamin C. most respondents favored  texture of noni ice cream at 69.9 %  and taste of noni ice cream at 54.50 % while products the ice cream by mixing 15% respectively noni fruit texture. Noni ice cream selling during one month resulted profits at 42.200 IDR. Analysis of feasibility noni ice cream was at 1.27 (> 1) which indicated that the business of ice cream noni products was feasible to be continued and carried out the development of the product. Keywords: business, ice cream, marketing, noni, processing  
EKSPRESI PROTEIN P53 PADA SEL TIG-3 SETELAH PERLAKUAN SINAR UV DAN EKSTRAK BIJI JUWET (Syzygium cumini) Roudlotul Jannah; Nashi Widodo
Biotropika: Journal of Tropical Biology Vol 2, No 5 (2014)
Publisher : University of Brawijaya

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Abstract

Tubuh terlindungi dari lingkungan luar karena adanya kulit. Adanya paparan lingkungan luar, seperti sinar UV dapat mempengaruhi sel pada kulit. Dalam jangka pendek dapat menyebabkan kulit terbakar serta dalam jangka panjang menyebabkan kanker atau penuaan dini. Salah satu cara yang biasa digunakan untuk melindungi sel kulit dari paparan sinar UV adalah dengan penggunaan antioksidan. Jambolan (S. cumini) merupakan salah satu tumbuhan yang memiliki kandungan antioksidan yang tinggi. Protein p53 merupakan salah satu protein yang bertugas dalam melindungi DNA dari kerusakan yang ditimbulkan setelah sel kulit terpapar sinar ultraviolet (UV). Penelitian ini bertujuan untuk melihat ekspresi protein p53 pada sel fibroblast TIG-3 setelah dipapar sinar UV serta pemberian ekstrak biji juwet. Sel dikultur dan dipapar sinar UV 30mJ/cm2 lalu diberi ekstrak biji juwet dengan dosis 0,1% dan 10%. Pemberian ekstrak biji juwet mampu meningkatkan ekspresi protein p53 pada sel TIG-3.   Kata kunci: ellagitannin, fibroblas, jambolan, p53, UV.
Dinamika Komunitas dan Potensi Bakteri Pendegradasi Linear Alkilbenzen Sulfonat Pembentuk Biofilm di Ekosistem Sungai Sunter Ahmad Ridlo; Suharjono Suharjono
Biotropika: Journal of Tropical Biology Vol 1, No 5 (2013)
Publisher : University of Brawijaya

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Abstract

Pencemaran air di ekosistem sungai banyak disebabkan oleh penggunaan deterjen dengan surfaktan anionik Linear Alkylbenzen Sulfonat (LAS). Komunitas bakteri pembentuk biofilm indegenous sungai tercemar deterjen diketahui memiliki kemampuan mendegradasi LAS. Tujuan penelitian ini untuk mempelajari dinamika dan diversitas komunitas bakteri pembentuk biofilm di ekosistem sungai serta potensinya dalam mendegradasi LAS. Penelitian meliputi isolasi bakteri dari sedimen sungai dengan metode pour plate dan uji potensi biodegradasi dalam sistem biakan kontinyu. Konsorsium isolat-isolat bakteri hasil isolasi diuji potensinya dalam mendegradasi LAS dalam media mineral sederhana yang mengandung 20 mg/L LAS dengan penambahan  sedimen batu sebagai substrat  dan perlakuan debit 50 dan 70 mL/jam. Densitas komunitas bakteri pendegradasi LAS dari bulan Desember 2012 hingga Februari 2013 relatif sama yaitu antara 19,7x104 cfu/g – 28,9x104 cfu/g, dengan indeks diversitas kurang dari 0,4. Konsorsium bakteri pada perlakuan debit 50 dan 70 mL/jam secara berturutan dapat mendegradasi LAS sebesar 51,23 % dan 76 % dengan waktu inkubasi 24 jam.  Didapatkan enam isolat bakteri pendegradasi LAS berdasarkan karakter fenotip berbeda spesies dengan nilai similaritas 51 %.   Kata kunci: bakteri, biofilm, degradasi, dinamika komunitas, LAS
Understanding Snake Bite Cases Pattern Related to Volcano-Seismic Activity: An Evidence in Bondowoso, Indonesia Nia Kurniawan; Ahmad Muammar Kadafi; Agung Sih Kurnianto; Franky Ardiansyah; Tri Maharani
Biotropika: Journal of Tropical Biology Vol 5, No 3 (2017)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2017.005.03.8

Abstract

Bondowoso located in a valley of Raung mountain roots in East Java, Indonesia. The eruption of Mt. Raung in 2015 not only cause loss of life, but also underlining the fact of unusual Snake Bite Cases (SBC), as recorded in the dr. Koesnadi Hospital. We conducted a thorough analysis of the SBC in 2015 along with the survey on all the incident location in January to March 2016. This study represented an improvement on the population of venomous snake in the agriculture area, and reached the highest peak before the eruption. The presence of continuous vegetation at the edge of watersheds become green corridor that functioned as the movement track for snakes in order to avoid the volcano effect.
Activity Test of VipAlbumin® against Blood Glucose Levels and Lymfhocytes Profiles in Balbc mice as Type 2 Diabetes Mellitus model Ganys Tri Silvana; Muhaimin Rifa'i
Biotropika: Journal of Tropical Biology Vol 3, No 1 (2015)
Publisher : University of Brawijaya

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Abstract

Activity Test of VipAlbumin® against Blood Glucose Levels and Lymfhocytes Profiles in Balb/c mice as Type 2 Diabetes Mellitus model   Ganys Tri Silvana1), Muhaimin Rifa’i2) 1) Biology Department, Faculty of Science, Brawijaya University, Malang, Indonesia   Abstract Diabetes mellitus (DM) has a prevalence that is increase from year to year, so we need an effective and economical drugs to cope. VipAlbumin® have high antioxidant content, so it can be used as an effective diabetes therapy. The purpose of this study was to determine the effect VipAlbumin® derived from Fish Cork (Channa striata) in decreasing blood glucose levels and assessing relative number of T cells. Mice models of  diabetes mellitus (DM) were created by injecting treptozotocin in  mice at the age of 5 days (streptozotocin in 1 ml of citrate buffer solution, a dose of 100 mg / kg BW). Mice were divided into 5 groups (Normal mice, DM, DM-D1, DM-D2 and DM-D3). After 3 weeks, the levels of blood glucose was examined and then oral therapy with VipAlbumin® was performed for 15 days. Profiles of CD4+, CD8+, and CD4+CD62L+ T cells were analyzed by flow cytometry by isolating splenic cells. One-way ANOVA was used to analyze the data. The differences between groups were considered significant at P<0.05. All results were presented as the mean of ± SD values of 5 mice in each group. In this experiment we showed that VipAlbumin® have a capability to prevent T cells activation by increasing naïve type status of both CD4 and CD8 population in mice model of DM. The number of CD4+ and CD8+ T cells decreased significantly in a dose of D3. Effective dose to decrease blood sugar in mice with type 2 diabetes is on the D3.   Keyword : Diabetes Melitus, Flowcytometry, Lympocytes, Streptozotocin, VipAlbumin®.
PERTUMBUHAN PADI HITAM DAN SERANGAN BEBERAPA HERBIVOR DI SAWAH PADI ORGANIK KECAMATAN KEPANJEN linda deviana cristanti; Endang Arisoesilaningsih
Biotropika: Journal of Tropical Biology Vol 1, No 5 (2013)
Publisher : University of Brawijaya

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Abstract

ABSTRAK Penelitian ini bertujuan untuk menentukan diversitas herbivor pengunjung padi hitam varietas Toraja di sawah padi organik di Kecamatan Kepanjen, Malang, menentukan gejala gangguan pertumbuhan padi hitam akibat serangan ringan herbivor, serta membandingkan pertumbuhan padi hitam akibat serangan tikus dengan tanaman tidak terserang. Pengamatan dilakukan di tiga petak sawah padi hitam organik pada empat umur yaitu 28, 48, 88, dan 112 hari setelah tanam (hst). Pertumbuhan padi hitam diamati tinggi rumpun, jumlah anakan, malai, spikelet dan biomassa. Pengamatan diversitas hewan pengunjung dengan  metode jelajah dan visual encounter di sisi tiap petak sawah padi hitam organik  menggunakan skor kelimpahan 0-4 pada jam 06.00-10.00 WIB. Diversitas hewan pengunjung ditentukan dengan kekayaan spesies, famili, dan ordo. Data pertumbuhan padi hitam yang terserang tikus dianalisis menggunakan software PAST dan SPSS. Hasil penelitian menunjukkan telah diamati enam famili herbivor pada fase vegetatif dan generatif yang mengunjungi sawah padi hitam di antaranya tikus (Muridae), burung pipit (Estrildidae), ulat penggerek batang (Pyralidae), lalat (Muscidae), lalat pucuk padi (Ephydridae) dan ngengat (Arctiidae). Serangan herbivor pengunjung terbesar pada umur 26 hst diakibatkan oleh Muride, dan fase generatif oleh Estrildidae. Walaupun pasca serangan tikus terjadi recovery pada pertumbuhan tinggi padi, namun jumlah malai dan spikelet padi menurun  10 %-55 % pada saat panen. Kata kunci: Padi hitam, herbivor, pertumbuhan, dan tikus.
Pengaruh Aplikasi Cendawan Mikoriza dan Perlakuan Pemberian Air terhadap Peningkatan Kadar Asiatikosida Tanaman Pegagan (Centella asiatica (L.) Urban) Roni Ramadhan; Ellis Nihayati; Sitawati Sitawati
Biotropika: Journal of Tropical Biology Vol 5, No 3 (2017)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2017.005.03.13

Abstract

Tanaman pegagan memiliki salah satu kandungan bahan aktif penting yaitu asiatikosida. Salahsatu cara untuk meningkatkan kadar asiatikosida tanaman pegagan adalah dengan aplikasi cekaman air dan cendawan mikoriza. Penelitian bertujuan untuk mengetahui pengaruh cendawan mikoriza dan cekaman air terhadap pertumbuhan dan hasil tanaman pegagan untuk meningkatkan kadar asiatikosida. Penelitian secara faktorial dengan rancangan acak kelompok yang diulang tiga kali. Faktor pertama adalah 3 taraf dosis cendawan mikoriza yaitu tanpa cendawan mikoriza, 15 g polibag-1 cendawan mikoriza dan 30 g polibag-1 cendawan mikoriza. Faktor kedua adalah 4 taraf perlakuan cekaman air yaitu 100%, 75%, 50% dan 25% kapasitas lapang. Hasil penelitian menunjukkan terdapat interaksi antara cendawan mikoriza dengan perlakuan cekaman air terhadap pertumbuhan dan hasil tanaman pegagan dalam meningkatkan kadar asiatikosida. Pada tingkat cekaman air 25% kapasitas lapang, perlakuan cendawan mikoriza 15 g polibag-1 dan cendawan mikoriza 30 g polibag-1 menghasilkan jumlah daun per rumpun, luas daun per rumpun, panjang tangkai daun per rumpun, panjang akar per rumpun, rasio tajuk akar dan kadar asiatikosida yang nyata lebih tinggi dibandingkan tanpa pemberian cendawan mikoriza. Pada perlakuan cendawan mikoriza 30 g polibag-1 dengan tingkat cekaman air 50% kapasitas lapang menunjukkan nilai kadar asiatikosida yang nyata lebih tinggi dibandingkan dengan tanpa perlakuan cendawan mikoriza, namun tidak berbeda dengan perlakuan cendawan mikoriza 15 g polibag-1.
Expression of Erythroid Progenitor Cells and Erythrocytes on Dexamethasone Induced-Mice Wira Eka Putra; Muhaimin Rifa&#039;i; Aris Soewondo
Biotropika: Journal of Tropical Biology Vol 3, No 1 (2015)
Publisher : University of Brawijaya

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Abstract

Dexamethasone (Dex) is synthetic corticosteroid, known as anti-inflamation drug to ameliorate autoimmune diseases. It worked by inhibiting production of proinflamatory citokines. The aim of this experiment was to confirm the effect of administration of Dex on erythroid progenitor cells, TER-119+VLA-4+ and erythrocytes, TER-119+VLA-4- expression from bone marrow compartments. Two weeks old BALB/c mice were used and grouped into 3 injection treatments of Dex with six replications i.e. no injection (control), 0.5 mg/kg BW (dose 1), 10 mg/kg BW (dose 2). To investigate the therapeutic effect of dexamethasone, the mice were sacrified on day-7. The bone marrow cells were isolated and analyzed by flow cytometry. Data analysis was confirmed with the Kruskal-Wallis test followed by Mann-Whitney test with significance level (α) of 0.05. The result showed that administration of Dex on BALB/c mice increase the expression of erythroid progenitor cells, TER-119+ VLA-4+ and erythrocytes, TER-119+VLA-4- from bone marrow.Key words: Dex, erythrocytes, erythroid progenitor cells
Studi Ekstrak Metanol Biji Mahoni terhadap MDA Serum Tikus Putih Pasca Induksi MLD-STZ Putri Puspitasari; Agung Pramana Warih Mahendra; Aulanni&#039;am Aulanni&#039;am
Biotropika: Journal of Tropical Biology Vol 1, No 6 (2013)
Publisher : University of Brawijaya

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Abstract

Diabetes mellitus (DM) merupakan suatu penyakit gangguan metabolik yang ditandai oleh peningkatan kadar glukosa darah melebihi batas normal atau hiperglikemia. Salah satu tumbuhan yang dimanfaatkan  masyarakat untuk pengobatan DM adalah  tanaman mahoni (Swietenia mahagoni). Bagian tumbuhan yang digunakan untuk pengobatan penyakit ini adalah bijinya. Namun potensi dari  ekstrak metanol biji mahoni belum banyak diketahui. Pada penelitian ini menggunakan tikus model DM dengan induksi MLD-STZ (Multiple Low Dose-Streptozotocin). Tikus dikelompokkan dalam 5 kelompok, yaitu kelompok kontrol negatif, kontrol positif, dan  kelompok  terapi 100, 250 dan 400 mg/KgBB yang sebelumnya telah diinduksi MLD-STZ. Metode yang digunakan dalam penelitian ini meliputi pembuatan tikus DM dengan injeksi MLD-STZ secara intraperitonial, pamberian terapi ekstrak metanol biji mahoni secara oral, uji kadar MDA serum. Hasil penelitian menunjukkan bahwa kadar MDA (Malonialdehide) serum yang didapatkan berdasarkan kelompok, yaitu 3,126; 10,245; 9,245; 7,203; 4,677 µg/ml. Dosis optimum untuk menurunkan kadar MDA adalah 400 mg/KgBB. Pemberian terapi ekstrak metanol biji mahoni mampu menurunkan kadar MDA serum tikus putih pasca induksi MLD-STZ. Kata Kunci: diabetes mellitus, MDA, MLD-STZ, pankreas
Review: Potency of Secondary Metabolite Componds from Soursop (Annona murricata) and Sugar Apple (Annona squamosa) as Plant-Based Pesticides for Controling Pests and Diseases of Plants Edi Tando
Biotropika: Journal of Tropical Biology Vol 6, No 1 (2018)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2018.006.01.5

Abstract

Indonesia is one country that is rich will flora and fauna which are biological resources. Plant secondary metabolites is a resource that is used for drugs. Besides can be extracted directly from the plant, also can be synthesized to obtain chemical compounds or their derivatives, through the development of cell culture. The impact of the use of synthetic pesticides / chemicals have an impact that is not good for health and cause disruption of the ecological balance. This paper aims to determine the potential utilization of secondary metabolites in plant soursop (Annona murricata) and sugar apple (Annona squamosa) as botanical pesticides to control pests and plant diseases. Plants soursop (Annona muricata) and sugar apple (Annona squamosa) has potential as a pesticide plant in controlling pests and diseases in plants. Results of phytochemical screening on extracts of leaves of the soursop (Annona muricata) found secondary metabolites: alkaloids, flavonoids, terpenoids, coumarin and lactones, anthraquinone, tannins, glycosides, phenols, phytosterols, and saponins, while the detection of the presence of a group of chemical compounds in seed extracts Soursop ( Annona muricata) and sugar apple (Annona squamosa), found secondary metabolites: alkaloids, flavonoids, polyphenols, terpenoids, coumarin, steroids, lactones, anthraquinone, tannins, glycosides, and saponins cyanadin. Natural pesticides are reducing pests, but is not directly Plant Pest Organisms (PPO) pests quickly. Natural pesticide application with the correct dose, can reduce pests, reduce costs and be environmentally friendly.

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