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Journal of Biomedicine and Translational Research
Published by Universitas Diponegoro
ISSN : -     EISSN : 25032178     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Journal of Biomedicine and Translational Research (JBTR) is an open access, international peer-reviewed journal that considers articles on: clinical medicine, molecular medicine, tropical medicine, infectious diseases, cardiovascular medicine, molecular biology, genetics, immunology, microbiology, biochemistry, and pharmacotherapy with particular interest on the link between clinical and basic research called translational research.
Arjuna Subject : -
Articles 4 Documents
 1 
Search results for , issue "Vol 11, No 2 (2025): August 2025" : 4 Documents clear
Detection of Pathogenic Leptospira in Sputum of Leptospirosis Patient with Pulmonary Hemorrhage. Handayani, Farida Dwi; Novipuspitasari, Lisa; Ahmed, Ahmed; Safari, Dodi; Hidajat, Muhammad Choirul; Soebandrio, Amin; Gasem, Muhammad Hussein
Journal of Biomedicine and Translational Research Vol 11, No 2 (2025): August 2025
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v11i2.28259

Abstract

Background: The pathogenic Leptospira species is the causative agent of leptospirosis, an endemic zoonotic disease in Indonesia. Misdiagnosis of the disease frequently occurs, as confirmatory diagnosis confined to highly specialized laboratories. As well, the pulmonary involvement of leptospirosis with hemoptysis is scarcely reported.Case Presentation: A 49 years-old male patient was admitted to the district hospital with acute febrile illness and a history of traveling to a malaria-endemic area in Borneo, Indonesia. Based on a chest X-ray result, the patient was clinically suspected to have pulmonary tuberculosis. However, the clinical manifestations of leptospirosis i.e. conjunctival suffusion, calf pain, and oliguria were present, and later hemoptysis was also reported. A clinical diagnosis of leptospirosis with pulmonary involvement was proposed. Immunochromatographic test (ICT)-rapid test for vivax/falciparum malaria and Ziehl-Neelsen (ZN) staining of sputum for tuberculosis results were both negative. Microscopic Agglutination Test (MAT), the IgM anti-Leptospira rapid test (lateral flow assay), and PCR amplification of both conventional and real-time (qPCR) were performed using various samples (serum, urine, and sputum). The MAT of acute single serum sample and rapid test were negative. Intriguingly, the PCR showed positive results in sputum and urine samples but not in the serum sample, highlighting the usefulness of leptospiral molecular detection to confirm further diagnosis.Conclusion: Molecular detection of pathogenic Leptospira in sputum samples can be considered for confirmatory diagnosis of leptospirosis patients with pulmonary hemorrhage. Likewise, the urine sample can be used as an option in the examination of severe leptospirosis.
Effect of Umbilical Cord Cell Isolate Administration on STRO-1 and CD 34 Expression of Dental Pulp Stem Cell Culture Nugraeni, Yuli; Rachmawati, Ranny; Az Zahra, Akyla Oktriza; Nugraha, Adelia Adzra
Journal of Biomedicine and Translational Research Vol 11, No 2 (2025): August 2025
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v11i2.26269

Abstract

Background: Dental Pulp Stem Cell (DPSC) is one of the ideal mesenchymal stem cells (MSCs) for the repair of damaged cells, as it has great proliferative potential and the ability to regenerate pulp-like complex dentins. However, DPSC has limitations in providing stem cell sources due to its small number. Therefore, it is induced with umbilical cord cell isolate (hUC-MSC) which is currently widely developed because of its advantages that are easy to obtain, have proliferative potential, and have anti-inflammatory effects. However, its influence and effectiveness on Dental Pulp Stem Cells is not widely known.Objective: To prove the effect of umbilical cord cell isolate on the expression of Stro-1 and CD 34 in Dental Pulp Stem Cell (DPSC) culture.Methods: This study is experimental laboratory with simple random sampling which is divided into 4 groups, namely 2 control groups and 2 groups of umbilical cord isolate administration which are observed for 12 hours and 24 hours. Observation of expression by immunohistochemical staining was then calculated using Qupath software in 5 fields of view.Results: The treatment group that was given umbilical cord cell isolate showed the highest increase in Stro-1 and CD 34 expression at 24-hour observation compared to the untreated group.Conclusion: There was an increase in the expression of Stro-1 and CD 34 in Dental Pulp Stem Cell cultures after being given umbilical cord cell isolate.
VEGF mRNA Expression in Epithelial Ovarian Cancer: Correlation with rs699947 Gene Variant Prameswari, Yuda Nabella; Suryandari, Dwi Anita; Sukmawati, Dewi; Yunaini, Luluk; Kodariah, Ria
Journal of Biomedicine and Translational Research Vol 11, No 2 (2025): August 2025
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v11i2.26156

Abstract

Background: Angiogenesis is the formation of new blood vessels, is crucial for cancer growth and metastasis, including in epithelial ovarian cancer (EOC). Vascular Endothelial Growth Factor (VEGF) regulates angiogenesis, and its elevated mRNA expression is linked to poor prognosis in cancer. Genetic variations, such as the rs699947 polymorphism in the VEGF gene, can affect VEGF expression and contribute to cancer progression.Objective: The primary aim of this study is to examine the distribution of the VEGF rs699947 polymorphism and its correlation with VEGF mRNA expression levels in patients with low-grade and high-grade EOC at Dr. Cipto Mangunkusumo Hospital, Indonesia.Methods: This research is a cross-sectional analysis involving 65 normal female whole blood samples and a total of 80 ovarian cancer biopsy samples, including 15 ovarian cysts as expression calibrators, along with 36 low-grade and 29 high-grade EOC samples. The distribution of genotypes and alleles of the VEGF rs699947 polymorphism was assessed through ARMS PCR analysis, while VEGF mRNA expression was quantified using real-time qPCR.Results: Significant differences were observed in both genotype (p<0,01) and allele (p=0,000) distributions between the normal and cases group. The relative mRNA expression of VEGF was significantly elevated in both low-grade and high-grade EOC. Individuals with the homozygous VEGF rs699947 AA genotype exhibited the highest mRNA expression compared to other genotypes. In contrast, individuals carrying the CC genotype showed the lowest correlation with VEGF mRNA expression in both low-grade and high-grade EOC.Conclusion: This study shows that the A allele of VEGF rs699947 is correlated with increased VEGF mRNA expression in EOC patients, particularly in those with the AA genotype. Conversely, the C allele may offer a protective effect against EOC, as the CC genotype is linked to lower VEGF mRNA expression. Genetic screening for VEGF rs699947 could facilitate early detection and inform targeted therapeutic strategies.
The Association Between Intake of Saturated, Monounsaturated, and Polyunsaturated Fatty Acids with Bifidobacterium Abundance Among Obese Adults Without Metabolic Syndrome Rustanti, Ninik; Sinthika, Euodia; Lestari, Endang Sri
Journal of Biomedicine and Translational Research Vol 11, No 2 (2025): August 2025
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v11i2.26630

Abstract

Background: Bifidobacterium is a key gut microbe that contributes to host metabolism, immunity, and intestinal integrity through SCFA production. Dietary fats are known to modulate gut microbiota, but evidence on the effects of specific fat types—SFA, MUFA, PUFA—on Bifidobacterium in obese adults without metabolic syndrome remains limited.Objectives: To examine the association between intake of dietary fat types and the abundance of Bifidobacterium among obese adults without metabolic syndrome.Methods: A cross-sectional study was conducted in Semarang, Indonesia, involving 60 obese adults (BMI ≥ 25 kg/m²) without metabolic syndrome. Dietary intake was assessed using a validated SQ-FFQ, and Bifidobacterium abundance was quantified using qPCR from fecal samples. Correlation and multivariate linear regression - adjusted for age, sex, and energy intake - were used to assess associations between variables.Results: Saturated fat intake was moderately and negatively correlated with Bifidobacterium levels (r = –0.464; p < 0.001), while total fat intake also showed a statistically significant, but weaker, negative correlation (r = –0.346; p = 0.007). PUFA intake showed a weak but statistically significant positive correlation (r = 0.269; p = 0.037), whereas MUFA intake was not significantly associated. Multivariate analysis identified SFA as an independent negative predictor of Bifidobacterium abundance.Conclusion: High intake of saturated fat is associated with decreased Bifidobacterium levels even in obese adults without metabolic syndrome, whereas PUFA may exert modest protective effects. These findings suggest that the type of dietary fat, rather than its quantity, plays a key role in modulating gut microbiota composition.

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