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Research Journal of Life Science
Published by Universitas Brawijaya
ISSN : -     EISSN : 23559926     DOI : https://doi.org/10.21776/ub.rjls
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Research Journal of Life Science (RJLS) is an open access publishes papers three times a year that emphasize the agriculture, fisheries, animal science, medicine, basic science and food technology. All articles are rigorously reviewed by reviewers. Articles should be created in a language that is easily understood by non-specialist readers in the topic of the article itself, but interested in research. The main objective of RJLS is to provide a platform for the international scholars, academicians and researchers to share the contemporary thoughts and innovations in the field of life science. RJLS aims to promote studies in life science and so become the leading international journals in life science in the world.This journal intends to provide facilities to advance the development and application of interdisciplinary and transdisciplinary research methodologies in life sciences.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 202 Documents
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Nematicidal Activity of Turmeric Extract against Nematodes Meloidogyne spp. Ulfa, Maria; Himawan, Toto; Tarno, Hagus
Research Journal of Life Science Vol 8, No 1 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.01.7

Abstract

In an effort to reduce the impact of synthetic nematicides, a new formula was developed using plant-based nematicides that are more environmentally friendly, one of the basic ingredients that can be used is turmeric. The purpose of this study was to determine the effectiveness of turmeric extract (Curcuma domestica) with various different solvents namely aquades, methanol, and n-hexane at concentration of 5%, 10%, 15% and 20% respectively in inhibiting the activity of Meloidogyne spp. The experiment used was a Completely Randomized Design (CRD). Each treatment was repeated 4 times with 4 controls so that there were 52 experimental units. The result of different research data was analyzed using Analysis of Variance (ANOVA) which was then further tested with Honest Significant Difference (HSD) at the 5% level if real, and t-test for in vivo test. Based on the tested extract, it was found that turmeric extract with various solvent (aquades, methanol, and n-hexane) was effective in inhibiting the activity of Meloidogyne spp. nematodes outside the roots (hatching eggs and penetration), turmeric extract didn’t affect the activity of Meloidogyne spp. in the roots (development and reproduction), vegetable nematicides which the most active in suppressing the population and inhibiting the activity of nematodes was turmeric extract using methanol as a solvent at a concentration of 20%.
Effectiveness of Organic Pesticides made from Cigarette Stub to the Attack Pest Intensity of Terminalia cattapa Yudanto, Yusuf Arya; Primartu, Syaviela Viagul Sams; Fuadiyah, Dinana Anissatul; Arifan, Fahmi
Research Journal of Life Science Vol 8, No 1 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.01.8

Abstract

The high consumption of cigarettes, especially in Indonesia, will produce harmful waste for the environment called cigarette stubs.  The waste of cigarette stubs reacts with any kinds of pathogenic fungus such as Fusarium oxysporum, Colletotrichum gloeosporiodes, Rigidoporus lignosus, and Sclerotium rolfsii. The usage of cigarette stubs as an alternative raw material for making organic pesticides can make cigarette stubs considerable as a recycled product that reduces the environment's waste. The results obtained by plants treated with biopesticides effectively resist pest attacks and support plants to naturally develop antibodies. The score of Intensity Attack (IS) from Terminalia cattapa plant applied by the biopesticides for 10 days observation results in the amount of 3.953%, with a total of 34 normal leaves and a total of 12 broken leaves. The other result of the Terminalia cattapa applied with chemical pesticides gives 12.31% of intensity attack and 12.18% of intensity attack without any treatment. This research concluded that tobacco could inhibit the attack pest of the Terminalia cattapa plant.
Coral Reefs Health Status in the East Java: a Case Study in Banyuwangi, Situbondo, Probolinggo Samuel, Pratama Diffi; Dewi, Citra Satrya Utama; Azmi, Nur Fadholi Ulul; Anam, M.Choirul; DP, Imas; Setyorini, Dewi Nur; Hariani, Dyah Fitri; Fatmawati, Riska
Research Journal of Life Science Vol 8, No 2 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.02.1

Abstract

Coral reef ecosystems are among the most productive natural biodiversity resources  in tropical coastal areas, within seagrass beds and mangrove forests. Besides, coral reefs have very high ecological impacts and economic value that are managed well and sustainably. Also, healthy ecosystems will undoubtedly provide more incredible benefits and values, so studies need to be conducted to determine the health of coral reefs. While P2O LIPI is an institution that is the guardian of coral reef health data in Indonesia, there is limited information related to the coastal areas of East Java that has not been widely reported. This study aimed to find out the percentage of living hard corals and the health status of the ecosystem at three reef sites: Banyuwangi, Situbondo, and Probolinggo. Field surveys were carried out in June-July 2019, using the Line Intercept Transect (LIT) method. The materials used in this study are Self-Contain Underwater Breath Apparatus-SCUBA diving equipment, roll meters, and an underwater camera. The results showed that the hard coral cover living in Banyuwangi accounted for 28.91%, Situbondo was 31.20%, and Probolinggo (34.77%). Therefore, this study interpreted that the coral reef ecosystem was a moderate category (25% - 49.9%).
Feeling Guilt and Happiness Police of The Regional Traffic Management Center (RTMC) of The East Java Regional Police to Their Families (Phenomenology Study) Wijaya, Yunus Adi; Hariyanti, Tita; Nasution, Tina Handayani
Research Journal of Life Science Vol 8, No 2 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.02.2

Abstract

Guilts is the first and foremost in the form of emotional outbursts. Guilts is not a very good motivator. It is more accurate to regard guilt as an internal condition and a negative feeling. Happiness is a feeling of satisfaction, that life is as it should be and all needs have fulfilled. This study aims to find meaning in the experience of feeling guilt and happiness police of the Regional Traffic Management Center (RTMC) to their families. Qualitative interpretive phenomenology approach was conducted an in-depth interview with semistructured questions on five police officers at RTMC by purposive sampling conducted in December 2018. Based on Interpretative Phenomenological Analysis (IPA), this study found two themes namely: feeling guilty to the family and feeling happy with family. The findings obtained were that members of the police at RTMC could be free from stress while working and were able to return and get support from the family. Being able to live happily, both happy at work and happy with the family that will reflect in the calmness in their life.
The Effect of Learning Tabletop Disaster Exercise (TDE) to Improve Knowledge among Nursing Students for Disaster Emergency Response Mukhamad Fathoni; Yuyun Yueniwati Prabowowati Wadjib; Widya Addiarto
Research Journal of Life Science Vol 6, No 1 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2019.006.01.4

Abstract

Today, the high incidence of disasters requires nurses to increase their role as disaster nursing in emergency response. This is in accordance with standards of the international council of nursing (ICN) that the nurse as a front line in providing treatment of disaster victims. One of the way to do in these situations is to improve the early preparedness in nursing students through disaster education, so that this plan needs a new method for effective and innovative disaster learning. One of the recommendation learning method is simulation with Tabletop Disaster Exercise (TDE). The purpose of this study was to analyze the effect of TDE learning for undergraduate nursing student’s knowledge in providing the management of victims in disaster response simulations. This study uses quasy experimental with pre-post test and control group design. The samples are 36 respondents were divided into two groups there are treatment and control groups so that each consisting of 18 respondents. The treatment group were given a TDE simulation, while the control group was given standard simulation and module. The results using paired t-test significantly showed that the differences value of knowledge before and after intervention with difference Pre-test 18 respondents mean 23.89±9.00 and Post test 18 respondents mean 81.67±8.04 Difference  Mean (CI 95%) = 57.78 (53.85-61.71) in each group indicated by the value (p = 0.000). The Effect Simulation Standards Knowledge Nursing Students with difference Pre test 18 respondents mean 26.39±9.04 and Post test 18  respondents mean 70.28±10.07 Difference  Mean (CI 95%) = 43.89 (38.59-49.18). The results from unpaired t-test to identify the difference of knowledge between the two groups after intervention has a significance value (p = 0.000). This means that there is significant difference of knowledge between TDE simulation and simulation.
The Effect of Water Treatment Models to Reduce Lead (Pb) Level on Freshwater Snail Filopaludina javanica Arfiati, Diana; Syahid, Nur; Anwari, Zaki; Aminin, Aminin; Kusriani, Kusriani; Herawati, Endang Yuli; Puspitasari, Asthervina Widyastami
Research Journal of Life Science Vol 8, No 2 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.02.3

Abstract

Lead is a kind of non-essential heavy metals included in the metal causing environmental pollution with persistent properties that might harm the consumers. This study aimed to determine the best method for reducing the lead level on the freshwater snail Filopaludina javanica using three various water treatment models. Soaking water treatment (6h, 12h, 18h, 24h), flowing water treatment (6h, 12h, 18h, 24h), and refreshing water treatment (6h, 12h, 24h). The lead level assay in both samples used Atomic absorption spectroscopy (AAS), and the physical and chemical parameters were measured, such as temperature, pH, and Dissolved Oxygen (DO). The lowest value of lead content on freshwater’s soft body snail and water sample respectively were soaking water treatment at 6h (0.64 ± 0.02 mg L-1) and 24h (0.0045 ± 0.0015 mg L-1); flowing water treatment at 24h (0.04 ± 0.007 mg L-1) and 18h (0.0036 ± 0.0009 mg L-1) and; refreshing water treatment at 24h (0.150 ± 0.011) and 12h (0.007 ± 0.001), with control 0.072 ± 0.00 mg L-1 and 0.067 ± 0.00 mg L-1. Therefore, the most effective model to reduce the lead content was flowing water treatment within 24h in the freshwater soft body snail and 18h in the water sample.
Phytochemicals and The Ability of Plantago major Linn. Extract to Inhibit The Growth of Aeromonas hydrophila Dewi, Annisa Farhana
Research Journal of Life Science Vol 8, No 2 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.02.5

Abstract

Aeromonas hydrophila isgram-negative bacteria that can harm humans and major animals with poikilotherm properties such as fish and shrimp. These bacteria can cause hemorrhages in fish until death, causing fish farming production to fail. The use of medicinal plants has been trusted by people all over the world to overcome various disease problems, one of which is a disease caused by bacteria. Plantago major L. is known that able to inhibit the growth of both gram-positive and gram-negative bacteria. This study aims to find out the compounds contained in Plantago major L., using phytochemicals screening, FTIR, UV-VIS and antibacterial activity against A. hydrophila.This study extracts Plantago major L. known to contain polar compounds such as phenols, flavonoids, saponins, and tannins. The result of FTIR and UV-VIS strengthen the presence of phenols and flavonoids. Furthermore, extract Plantago major L. is known to be able to significantly inhibit the growth of A. hydrophila (P<0.05).
Effect of Histatin-1 Proteins to Fibroblasts Growth Factor-2 (FGF2) Concentrate and Fibroblasts Cell Migration on Human Gingival Fibroblast (HGF) Culture Dewi, Ni Ketut Ayu Feriyanti; Permatasari, Nur; Sujuti, Hidayat
Research Journal of Life Science Vol 8, No 2 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.02.4

Abstract

The purpose of this study is to determine the effect of histatin-1 protein increasing the concentration of fibroblast growth factor (FGF2) and fibroblast cell migration in human gingival fibroblast (HGF) culture. The HGF cell culture used was obtained from odontectomy patients and was the result of the 4th passage. Cell culture was divided into 4 groups: HGF control group (without treatment) and treatment groups with 5µM, 10µM, and 20µM concentration of histatin-1 respectively. The amount of fibroblast growth factor 2 (FGF2) was quantified by using ELISA method. In vitro fibroblast cells migration was measured using scratch/wound healing assay method. FGF2 concentration and HGF cell migration were measured at hour 0, 3, 11, 24, and 48. The results of the One Way Anova statistical test difference between FGF 2 (ΔFGF2) at hour 24 showed a value of P = 0.042 (P≤0.05) and HGF cell migration at hour 11 showed a P value = 0.003 (P≤0.05). The conclusion of this study is that Histatin-1 Protein is able to increase the concentration of Fibroblast Growth Factor 2 (FGF2) and the migration of Human Gingival Fibroblast cells.
Diversity and Abundance of Insect on Shallot (Allium Ascalonicum L.) with Integrated Pest Management (IPM) and Conventional Patterns Manna, Dorkas Wantiawati; Afandhi, Aminudin; Tarno, Hagus
Research Journal of Life Science Vol 8, No 3 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.03.3

Abstract

Research on diversity and abundance of insect on shallot with Integrated Pest Management (IPM) and conventional patterns has been carried out. This study aims to analyze diversity and abundance of insects, damage level of shallot, and relationship between diversity and abundance of insects with growth and production of shallot in IPM and conventional land. This research was conducted in Junrejo village, Batu City in September to December 2017 while identification of insect was carried out at the Laboratory of Plant Pest and Disease, University of Brawijaya. This study uses Yellow pan trap and Pitfall trap methods. The result showed diversity index (H') of IPM land was 2.50 and of conventional land was 2.67, which both are moderate diversity. The abundance of insect on IPM land is higher (8672 individuals) compared to conventional land (3475 individuals). The level of crop damage is lower in IPM land compared to conventional land, while shallot growth and production is higher in IPM land compared to conventional land.
Indigenous Endophytic Bacteria Potentials to Control Black Rot Disease on Cabbage Towards the Development of Organic Vegetables Santoso, Verisca Agil Winanda; Ramadhani, Fatchuliani Safitri; Apriyani, Aliffia Nuraita; Aini, Luqman Qurata
Research Journal of Life Science Vol 8, No 3 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.03.8

Abstract

Organic farming plays an important role in achieving SDGs 2030, especially on point 2, 3, 13 and 15. Nevertheless, agriculture commodity often meets challenges in organic farming due to plant pests, for instance: on cabbage. Black rot disease on cabbage causes serious losses up to 70%. Most of farmers use chemical pesticides which leads to lots of negative impact towards the environment. Thus, its essential to control the environmentally friendly by applying bio control agents of indigenous endophyte bacteria instead. This article was aimed to examine the indigenous endophyte bacteria ability which excellences on organic cabbage as well as generating black rot caused by the bacterial pathogen X. campestris. Narrative reviews along was done through finding out the related material from International Journal articles. The result showed that there were indigenous endophyte bacteria which was able to be bio control agents towards black rot disease on cabbage, namely Pseudomonas fluorescens, Bacillus subtilis, and Streptomyces sp with effectivity level between 37-63% within resistance zone 1.44 up to 2.4 cm. Indigenous endophyte bacteria on organic cabbage had various kinds antagonist agents especially on Bacillus sp. and Pseudomonas sp. that able to resist the disease pathogen both in vitro and in vivo.

Page 16 of 21 | Total Record : 202

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