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Research Journal of Life Science
Published by Universitas Brawijaya
ISSN : -     EISSN : 23559926     DOI : https://doi.org/10.21776/ub.rjls
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Research Journal of Life Science (RJLS) is an open access publishes papers three times a year that emphasize the agriculture, fisheries, animal science, medicine, basic science and food technology. All articles are rigorously reviewed by reviewers. Articles should be created in a language that is easily understood by non-specialist readers in the topic of the article itself, but interested in research. The main objective of RJLS is to provide a platform for the international scholars, academicians and researchers to share the contemporary thoughts and innovations in the field of life science. RJLS aims to promote studies in life science and so become the leading international journals in life science in the world.This journal intends to provide facilities to advance the development and application of interdisciplinary and transdisciplinary research methodologies in life sciences.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 202 Documents
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Fourier Transform Infrared (FTIR) Spectroscopy Method for Fusarium solani Characterization Hasanah, Ifa Maulidah; Martosudiro, Mintarto; Aini, Luqman Qurata; Prillianti, Kestrilia Rega; Prabowo, Matheus Randy
Research Journal of Life Science Vol 9, No 1 (2022)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2022.009.01.3

Abstract

The detection and identification of microorganisms using spectroscopy techniques promise to be of great value because of their sensitivity, rapidity, low expense, and simplicity.  In this study, we used FTIR spectroscopy for the characterization of Fusarium solani. PCR amplification of DNA extracted from these isolates showed the possibility of amplifying PCR products with sizes 559 bp using the ITS1-ITS4 primers. Based on phylogenetic tree analysis, the isolate of F. solani showed a closely relationship to Fusarium solani isolate MN (MH300495.1) with 99.63% similarity.  The study is focused on the carbohydrate structure which can be analyzed in the range of 900 to 1200 cm-1 of FTIR wavenumber.  The spectra of our samples share similarities with one another, although small differences occur in the absorbance value. The band at 1027 cm-1 is assigned to the C-O stretching of glycogen. Meanwhile, at 1042 cm-1 is interpreted as carbohydrate C-O stretching as well. The band around 1073 cm-1 might arise from both chitin C-C stretching and phosphate stretching of nucleic acids. Other vibrations associated with chitin are also found at 1115 cm-1 and 1151 cm-1 which are assigned to C-O-C symmetric stretching and C-O-C asymmetric stretching, respectively.
FTIR (Fourier Transform Infrared) Spectroscopy Method for Bacillus cereus Characterization Silfiani, Intan; Abadi, Abdul Latief; Aini, Luqman Qurata; Prillianti, Kestrillia Rega; Prabowo, Matheus Randy; Dianti, Sylvia Herli
Research Journal of Life Science Vol 9, No 2 (2022)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2022.009.02.3

Abstract

One of the potentials of Bacillus cereus bacteria is as an inducer of systemic resistance in plants against plant diseases. FTIR spectroscopy is a traditional method extensively utilized in the pharmaceutical industry, since it is fast, non-destructive, and requires minimal sample preparation. In this study, the indicators that were effectively discovered were the functional groups that compose carbohydrates and lipids. The absorption region to be analyzed for the functional groups that compose carbohydrates is in the wavenumber region of 1200-800 cm-1, whereas the functional groups that compose up lipids are in the wavenumber range of 3020-2800 cm-1 and 1800-800 cm-1. The signal produced on the spectral signature of B. cereus isolates revealing the functional groups that compose carbohydrates is at the absorption values of 1185, 1132, 1122, 1098, 1078, 1056, 1010, and 973 cm-1. This is analogous to the notion that the cell wall of B. cereus is composed of peptidoglycan which contains carbohydrates. While the absorption values that indicate the functional groups that make up lipids are 2969, 2955, 2925, 2870, 2846, 1759, 1733, 1471, 1455, 1234, 895 cm-1, which according to the literature suggests that the body structure of B. cereus contains 2-3% of the dry weight of bacterial cells and consists of neutral lipids and phospholipids.
Identification of Aflatoxigenic Fungi in Myristica fragrans using V8 and CAM Media Arifah, Fitri; Aini, Luqman Qurata; Muhibuddin, Anton; Dianti, Sylvia; Prabowo, Matheus Randy
Research Journal of Life Science Vol 9, No 3 (2022)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2022.009.03.3

Abstract

Nutmeg is a spice that might be susceptible to being infected with aflatoxigenic fungi. Aflatoxins are mycotoxins synthesized by certain strains of Aspergillus section Flavi. However, not all strains are able to produce aflatoxins. The approach frequently employed for this survey comprises the cultivation of strains in a suitable liquid or solid medium and their subsequent extraction and analysis for the presence of aflatoxins. To date, very few studies have been conducted on the identification of contaminate and aflatoxigenic fungi in Myristica fragrans. Therefore, this study aimed to identify the contaminate and aflatoxigenic fungi in Myristica fragrans growing on V8 and CAM Media. Isolation of the fungus was carried out by direct agar plating. Lasiodiplodia theobromae, Aspergillus niger, A. tamarii, Penicillium citrinum, Rhizopus delemar, A. nomiae, and A. aflatoxiformans were recovered from nutmeg kernels sampled from North Minahasa, North Sulawesi, Indonesia. The findings of fungal isolation demonstrated that L. theobromae was the most prevalent form of fungus detected in the nutmeg kernels (50%), followed by A. niger (38.13%) and A. tamarii (10%). A. nomiae and A. aflatoxiformans were the aflatoxigenic fungi isolated from M. fragrans kernels.
Diversity of Ant Spesies (Hymenoptera: Formiciidae) in Refugia Plants Combination on Ratoon Sugarcane Mawaddah, Rabbiana; Rahardjo, Bambang Tri; Mudjiono, Gatot
Research Journal of Life Science Vol 9, No 1 (2022)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2022.009.01.4

Abstract

This research aims to examine the effects of several refugia plant treatments on the diversity and number of ants in the ratoon sugarcane system. The diversity and abundance of ants in the plantation's surrounding habitats can fluctuate. Three combinations of flowering plants were used in the study: Turnera subulata Sm (Malpighiales: Passifloraceae), Cosmos sulphureus (Asteraceae: Asteraceae), and Zinnia elegans Jaqc (Asterales: Asteraceae). Ant samples were collected using yellow sticky traps and traps and yellow pan traps. R. software was used to examine all of the data. 4 subfamilies, 14 genera, and 25 species: Diacamma sp., Technomyrmex sp., Camponotus sp.1., Iridiomyrmex sp., Delichoderus sp., Leptogenys sp.; Odontomachus sp., Polyrachis sp., Pheidole sp., Tetramorium sp., Monomorium sp., Crematogaster sp., Oecophylla smaragdina was identified from the report's results, this included 1-86 individuals. Tetramorium sp. and Odonthomachus sp. were the most common ants found at the study site. Even before compared to the other treatments, the combination of refugia and Z. elegans, C. sulphureus, T. subulata treatment used to have a significant impact on the presence of ants, increasing the population by 21%. This study discovered that combining refugia could increase ant diversity in ratoon sugarcane. The diversity index value for all treatments was almost the same, in the range of 2.-9-2.82, indicating that the main composition of refugia was moderately diverse.
Percentage of Hard Coral Cover and Coral Recruitment on in Bangsring Beach, Banyuwangi Regency Samuel, Pratama Diffi; Fakhri, Muhammad; Dewi, Citra Satrya Utama; Ellona, Jessica; Anam, M. Choirul
Research Journal of Life Science Vol 10, No 1 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.01.3

Abstract

One of the tourist attractions at Bangsring Beach is the condition of the coral reef ecosystem and unique marine biota that tourists can enjoy by snorkeling and diving. Based on research (Ekawati et al., 2021) using remote sensing, the area of live coral reefs on Bangsring Beach is as follows; 2005; 3.38, 2008; 2.61, 2011; 3.7, 2017; 3.98, 2020; 6.1 Ha). It can be said that the area of coral reefs increases yearly, although, in 2008-2011, it decreased by 32%. The results of the economic valuation explain that the (Total Economic Value) of the coral reef ecosystem in Bangsring Beach is 38.2 billion Rupiah/year or 2.9 billion Rupiah hectares/year. The use-value that has the largest economic value comes from tourism utilization of 25 billion or 60% of the Total Economic Value. The coral community structure includes Coral Branching, Coral Encrusting, Coral Massive, Coral Submaasive, and Coral Mushroom. Mushroom Coral is found solitary and shaped like a mushroom. The research data shows the average value of Bangsring Fish House cover is 82.5%. Fish house cover here is seen and assessed based on the presence of hard corals, soft corals, and algae. The average percentage of hard corals growing at the Bangsring Fish House was 30%, soft corals 21.5%, and algae 17.5%. Found 21 types of coral such as; Acropora, Favia, Turbinaria, Symphyllia, Goniastrea.
Colony Growth, Sporulation, and Viability of Entomopathogenic Fungus Beauveria bassiana (Balsamo) Vuillemin on Various Agricultural Waste as Growing Media Kurniawan, Irvan; Afandhi, Aminudin; Tarno, Hagus
Research Journal of Life Science Vol 9, No 2 (2022)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2022.009.02.4

Abstract

Beauveria bassiana is an entomopathogenic fungus that is widely produced for the benefit of biological control agents of various insect pests. The current production that is expected is to use cheap media but produce quality isolates. This study aims to determine colony growth, sporulation and viability of Beauveria bassiana conidia in several agricultural waste media. The experiment was compiled in a complete randomized design and replicated four times. B. bassiana in rice bran media added with cricket flour  had the fastest colony growth while B. bassiana in rice husk media was the lowest, lower than the control treatment. In  wheat bran media added cricket flour has slower colony growth than rice bran with cricket flour media similar to control treatment but has the second highest sporulation after rice bran with cricket flour media. The highest viability belongs to B. bassiana which is grown on rice bran with cricket flour media. The production in each treatment medium has a real effect but the addition of cricket flour has a significant influence in the production of quality isolates.
Association between Predatory Arthropods and Weeds on Sugarcane Plants Devi, Mia Prastika; Rahardjo, Bambang Tri; Tarno, Hagus
Research Journal of Life Science Vol 9, No 3 (2022)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2022.009.03.4

Abstract

While a large number of farmers in the tropics consider weeds to be just a nuisance, some weeds can potentially have benefits for cultivated crops. Weeds around cultivated plants can act as a biocide, soil improvement, and a food source for humans and animals, as well as a habitat for some insects. Based on the regression analysist, weed density has a relationship to the diversity of predatory arthropod species (R2= 0.026; P<0.001) and the abundance of individual predatory arthropods (R2= 0.010; P<0.001). Beneficial weeds around sugarcane plantations create mutually beneficial interactions. Weeds can be used as a place to live, reproduce, and produce nutrients for predatory arthropods. Research on the role of weeds against natural enemies from predators in sugarcane agroecosystems is also rarely studied. In this study, the focus was on the association of weeds with the diversity and abundance of predatory arthropods in the sugarcane agroecosystem.
Yield and Its Components of Shallot (Allium ascalonicum L.) Influenced by the Application of Amino Acid Fertilizer And Triacontanol Putra, Anggara Ista; Waluyo, Budi; Nihayati, Ellis
Research Journal of Life Science Vol 9, No 1 (2022)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2022.009.01.5

Abstract

Shallot are one of the agricultural products with a relatively high demand for the consumer market in Indonesia, but the productivity figure is relatively low compared to other countries (FAO, 2021). This study seeks to explore the effectiveness of the application of amino acid fertilizer and triacontanol in increasing the yield and yield components of shallot. The study was conducted in Karangploso District, Malang, from April to August 2021. In a randomized block design, this study used two factors: amino acid fertilizers (A1: 1 g plot-1; A2: g plot-1; A3: 5 g plot-1) and triacontanol (T0: 0 ppm; T1: 5ppm; T2: 10 ppm). Each combination was repeated three times. As a result, amino acid fertilizers can significantly affect the number of shallot bulbs, yields per hectare, economic weight, and economical weight loss. A significant effect was seen in the application of triacontanol on shallot yields per hectare and economic weight. The interaction of characters of bulb length was shown in the combination of 5-10 ppm triacontanol with amino acid fertilizer. The best response ranged from 10.64% to 11.67% in fertilizer with 5 g plot-1 of an amino acid fertilizer. As the doses of amino acid fertilizer in combination with triacontanol increased, the total bulb fresh weight grew as well, in which 5 g plot-1 of amino acid fertilizer could increase the weight by 45,23-45,43 g clump-1, which was greater than the response of the 1 g plot-1 amino acid fertilizer combination.
Phytoplankton Composition in White Shrimp (Litopenaeus vannamei) Pond Culture Infected White Feces Disease (WFD) Anjaini, Jefri; Simangunsong, Tohap; Fadjar, Mohamad
Research Journal of Life Science Vol 10, No 1 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.01.4

Abstract

The aim of this study is to find out the composition and condition of phytoplankton in the white shrimp (L. vannamei) culture inffected by the white feces disease so that one of the indicators of the onset of the white feces disease can be known. This research uses descriptive methods. The observed parameters are the identification of species diversity, abundance, diversity index and dominance index of phytoplankton. The sampling was conducted at three locations, Tuban area, Situbondo area, Lamongan area. The results of the study showed observations in Lamongan area, the total abundance of phytoplankton was 473 cells/L. Situpondo areas abundantity of phytoplankton total was 633 cels/L and Tuban areas abundance was 887 cells/L. In Lamongan area, the diversity index was 1,009. In Situbondo areas diversity Index was 1,013. In Tuban area, diversity indexes were 1,082. In Lamongan area, a dominance index was 0.477. In Situpond area, it was obtained a domination index of 0.544. The genus that dominates the three ponds are Cyclotella and Navicula. At all three locations have high variety of species and abundance of the genus members of the filum Cyanophyta and Bacillariofhyta are detrimental, the genuses that are found are indicators of contaminated waters. The dominance of Cyanophyta (blue-green algae) is also an indicator of the White Feces Disease.
Fusarium Rot Biological Control of Citrus caused by Fusarium oxysporum Dewi, Ovilya Kusuma Minarma; Abadi, Abdul Latief; Widyaningsih, Sri
Research Journal of Life Science Vol 9, No 2 (2022)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2022.009.02.5

Abstract

Fusarium oxysporum is a pathogen that causes Fusarium rot disease on citrus plants. The F. oxysporum is a soil-borne pathogen whose fungicide is not effective against it and difficult to cure. Thus, the use of Trichoderma spp. as one of the most effective and well-known biocontrol agents against many plant diseases in agriculture is needed. To test Trichoderma capabilities on the specific pathogen, method of this study consisted of isolation and rejuvenation of F. oxysporum and Trichoderma, morphological identification of the fungus, in vitro test of Trichoderma antagonistic ability against F. oxysporum on PDA medium, and in vivo test conducted in a green house on Rough Lemon (RL) and Japansche Citroen (JC) rootstock seeds to calculate the total disease incidence using a formula. The result of in vitro test of this study shows that 3 Trichoderma isolates (TJ, TKH, and TST) were able to inhibit the growth of F. oxysporum on PDA medium by 65.56%, 62.99%, and 61.19%, respectively. While in vivo test shows that the treatment of TJ isolates on RL seeds and TKH on JC seeds shows lowest disease incidence percentage of 3.33%. Therefore, this study proves that Trichoderma can be used as a biocontrol agent in controlling Fusarium rot disease in citrus plants. However, further research is needed to detect which Trichoderma species TJ and TKH isolates contain.

Page 18 of 21 | Total Record : 202

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