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Research Journal of Life Science
Published by Universitas Brawijaya
ISSN : -     EISSN : 23559926     DOI : https://doi.org/10.21776/ub.rjls
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Research Journal of Life Science (RJLS) is an open access publishes papers three times a year that emphasize the agriculture, fisheries, animal science, medicine, basic science and food technology. All articles are rigorously reviewed by reviewers. Articles should be created in a language that is easily understood by non-specialist readers in the topic of the article itself, but interested in research. The main objective of RJLS is to provide a platform for the international scholars, academicians and researchers to share the contemporary thoughts and innovations in the field of life science. RJLS aims to promote studies in life science and so become the leading international journals in life science in the world.This journal intends to provide facilities to advance the development and application of interdisciplinary and transdisciplinary research methodologies in life sciences.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 202 Documents
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In Vitro Exploration of Endophytes Bacteria from Platycerium Elephantotis Plants as Nitrogen Fixers and Phosphate Solubilizers Pamungkas, Bayu Aji; Aini, Luqman Qurata; Abadi, Abdul Latief; Trianti, Irisa; Syib`li, Muhammad Akhid; Setiawan, Adi
Research Journal of Life Science Vol 10, No 2 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.02.4

Abstract

Platycerium elephantotis, an African epiphytic fern thriving in Indonesia, is prized for its ornamental value and medicinal potential. It aids in fern succession without harming its host, obtaining nutrients from leaf litter and roots. Special water-storing tissues and CAM photosynthesis enhance its resilience. Endophytic bacteria in its roots likely contribute to nutrient absorption, including phosphate solubilization and nitrogen fixation, similar to other fern species. Studies on these bacteria are crucial for enhancing agricultural practices by reducing chemical inputs and promoting plant growth as plant growth-promoting bacteria (PGPB), particularly in nitrogen fixation and phosphate solubilization. The research was conducted from November 2023 to January 2024. Plant samples were collected from Arjuna Park Conservation Garden, Malang, and endophytic bacteria were isolated using serial dilution and nutrient agar media. Hypersensitivity, phosphate solubilization, and nitrogen fixation tests were conducted to assess bacterial traits. The study obtained six bacterial isolates (PE 5, PE 7, PE 24, PE 25, PE 30, and PE 49) from P. elephantotis with potential as biofertilizers due to their nitrogen-fixing and phosphate-solubilizing abilities. The diverse colony morphologies indicate different bacterial species. All isolates are rod-shaped (bacilli). Gram staining shows that PE 25 and PE 49 are gram-negative, while PE 5, PE 7, PE 24, and PE 30 are gram-positive.
Incidence of Extended Spectrum Beta-Lactamase (ESBL) of Escherichia Coli Isolated from the Feces of Dairy Cattles in Blitar Dameanti, Fidi Nur Aini Eka Puji; Amri, Indah Amalia; Roosdiana, Anna; Pratama, Alfaro Rikko; Izofani, Safira; Fahmiantika, Reza; Tedja, Dhaneswara; Sutrisno, Rahayu; Akramsyah, Muhammad Ali
Research Journal of Life Science Vol 10, No 1 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.01.5

Abstract

Antibiotic resistance (AMR) has caused a serious global threat to human health, animal health and food security. The antibiotic resistance problem, especially in Gram-negative bacteria, is increasingly widespread in various areas and has increased mortality, morbidity, and incidence. This study aims to determine the incidence of ESBL E. coli and the AMR and MDR properties of E.coli isolated from the feces of dairy cattles in Blitar, which have not been reported. This research was conducted from June to September 2022 with 60 samples of dairy cattle feces in Blitar. Samples were isolated and identified until confirmed that 55 (91.6%) samples were E. coli. All samples showed AMR character with 100% streptomycin, 87.27% chloramphenicol, 85.45% ampicillin, 72.73% cefotaxime, 40% tetracycline, 21.82% ciprofloxacin, and 14.55% sulfamethoxazole-trimethoprim. Forty-nine samples were MDR, with 10.9% resistant in three groups, 34.5% in four groups, 29.1% in five groups, 10.9% in six groups, and 3.6% in seven groups. MDR E. coli isolates were then confirmed for ESBL using the DDST test. The incidence of ESBL-producing E. coli from dairy cattle feces in Blitar was 40% (22 isolates). The results of this study can be a warning in the human health, animal health and food security.
Development of Cadra cautella Walker (Lepidoptera: Pyralidae) on Peanuts, Almonds, and Cashews Rahayu, Fitria Ariani; Astuti, Ludji Pantja; Rizali, Akhmad
Research Journal of Life Science Vol 10, No 2 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.02.5

Abstract

Cadra cautella Walker pest (Lepidoptera: Pyralidae) is a group of significant pests that can attack various stored products. The presence of C. cautella can cause damage to stored products, i.e. holes, contamination, and weight loss of stored products. This study aimed to determine the development time and life cycle of C. cautella in peanuts, almonds, and cashews. This research consisted of three diets repeated ten times and arranged in a Completely Randomized Design. This research carried out by preparing 30 eggs (0–24 h old) in each insect diet. The observed variables consist of the development of each phase (eggs, I-V instar larvae, prepupae pupae, adult), life cycle, longevity, fecundity, and fertility. The result showed that the diet type significantly affected the development of C. cautella. The developmental time and life cycle of C. cautella on cashews was shorter than on peanuts and almonds. Furthermore, the fecundity and fertility of C. cautella on cashews was higher than on peanuts and almonds. The results showed that cashews were of the suitable diets for the development of C. cautella.
Resistance of two sugarcane genotypes towards Sporisorium scitamineum Scoring Using Novel Method on Sugarcane buds tissue and Phenol Plant dynamics Supriyono, Supriyono; Abadi, Abdul Latief; Syib’li, Muhammad Akhid; Hidayah, Nurul
Research Journal of Life Science Vol 10, No 2 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.02.1

Abstract

Smut disease caused by a basidiomycete fungus, Sporisorium scitamineum, is one of major diseases on sugarcane worldwide. The disease could cause considerable yield losses up to 60% on susceptible varieties. Understanding of resistance characteristics of sugarcane clones is essential to support development of resistant sugarcane varieties to smut fungus infection. This study aimed to determine characteristics of sugarcane genotypes J19R17-325/15/3-9 and ASA Agribun toward S. scitamineum infection and the role of phenolic compound on defense mechanism of sugarcane to smut infection. The research consisted of four treatments and was arranged according to the randomized block design with five replicates. The results revealed that J19R17-325/15/3-9 produced higher phenolic compound (0,192 mg/gr) rather than ASA Agribun (0,182 mg/gr). In addition, the distribution of fungal mycelia within sugarcane tissues on J19R17-325/15/3-9 was also lower than those on ASA Agribun. These results indicated that J19R17-325/15/3-9 might have resistance properties to S. scitamineum infection.
The Use of Beneficial Microbes Tolerant of the Pyraclostrobin Fungicide, in Controlling the Pathogen Colletotrichum, the Cause of Anthracnose Disease in Chili Plants Tubagus, Satria Wahyu; Syib’li, Muhammad Akhid; Abadi, Abdul Latief
Research Journal of Life Science Vol 10, No 3 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.03.1

Abstract

Global production of chilies is significantly influenced by various biotic and abiotic factors. anthracnose is likely the main inhibiting factor reducing chili productivity in various countries. The use chemicals still formed the basis of disease control. Trichoderma sp. and FB1 bacterial is one such tolerant beneficial microbe belonging to the fungus and bacteria group that can survive under chemical pressure and exhibit antagonistic effects against the pathogen Colletotrichum sp. by the in vitro assay, it was found that in the antagonistic of Colletotrichum sp. with bacteria FB1 and Trichoderma sp., there was a significant difference, that the growth of Colletotrichum sp. could be inhibited by 69%. In the compatibility assay of bacteria FB1 and Trichoderma sp., no significant antagonistic response was found from both beneficial microbes. In the fungicide tolerance test of all isolates, it was found that beneficial microbes could still grow on growth media with a mixture of pyraclostrobin fungicide at a dose of 0.25 ul/ml. FTIR analysis showed the functional groups of the secondary metabolites of both the antagonistic bacteria and the antagonistic fungi had very high similarity in O-H and C=O groups. However, further research is needed on the mechanism of the secondary metabolites of the beneficial microbes involved in the inhibition of the pathogen Colletotrichum sp. 
Effect of Weather Conditions to Vascular Streak Dieback Disease Severity on Some Cocoa Clones with Different Resistant Levels Aini, Febrilia Nur; Aini, Luqman Qurata; Syibli, Muhammad Akhi; Sari, Indah Anita; Susilo, Agung Wahyu; Setyawan, Bayu; Malik, Abdul; Budiman, Aris
Research Journal of Life Science Vol 10, No 2 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.02.2

Abstract

Vascular streak dieback is a major disease in cocoa trees that affects at in various stages, and effect in significant yield losses. This study aimed to identify the area under the disease progress curve (AUDPC), infection rate (r), and the influence of weather factors on the development of vascular streak dieback disease caused by Ceratobasidium theobromae in some cocoa clones with different resistant levels. The research used a randomized complete block design consisted six clones as treatment and each treatment consisted of three replications. Disease severity was observed on six different cocoa clones and observations were conducted monthly. The observation of disease severity was observed monthly for 12 months and then used to analyze AUDPC and infection rate. Monthly data on the weather conditions were taken from the climatology station of the Indonesian Coffee and Cocoa Research Center, East Java. The results showed that Sulawesi 01 and ICCRI 09, categorized as resistant clones, showed low AUDPC values, 0.02 and 2.85 respectively. The cocoa clones Sulawesi 02 and MCC 02, categorized as tolerant clones, had higher AUDPC values, 19.87 and 34.27 respectively. The cocoa clones BCL and BL 50 showed the highest AUDPC values, 277.05 and 265.70 respectively. Path analysis on resistant clones indicated that rainfall is the most influential weather factor in increasing the severity of vascular streak dieback, followed by temperature. Higher relative humidity can significantly reduce disease severity, while sunlight and wind speed have smaller effects. In contrast, path analysis on susceptible clones showed that temperature, rainfall, relative humidity, and wind speed all positively influence the severity of vascular streak dieback. At the same time, sunlight has a mitigating effect.
Pathogenicity Test and Molecular Characterization of Colletotrichum spp. Fungi Causing Apple Fruit Rot Disease in Malang Regency Maknunin, Lu'lu'il; Abadi, Abdul Latief; Syib'li, Muhammad Akhid
Research Journal of Life Science Vol 10, No 3 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.03.4

Abstract

Apple (Malus sylvestris Mill) is a key commodity in Malang Regency, Indonesia, because of its role in promoting the agrotourism sector. But fruit rot caused by Colletotrichum spp. poses significant threats to production and its existence. This study aims to identify the causal agents of fruit rot in apple orchards in Pujon District, Malang, using morphological and molecular approaches. Four isolates suspected to be Colletotrichum spp. were obtained from symptomatic apple fruits and characterized through macroscopic, microscopic, and molecular methods, including ITS sequencing. Pathogenicity tests revealed that all isolates induced symptoms on healthy apples, with Colletotrichum siamense confirmed as the primary pathogen via BLAST analysis. These findings emphasize the need for accurate morphological and molecular identification to develop effective management strategies. Further research is recommended to examine the diversity of Colletotrichum species in Malang Regency to inform broader disease control strategies.
Biodiversity and Distribution Rice Arthropods in Malang Indonesia Istiqomah, Rina Nur; Tarno, Hagus; Mudjiono, Gatot
Research Journal of Life Science Vol 10, No 3 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.03.5

Abstract

Climate change is closely related to changes in abiotic conditions. These changes could have an impact on agricultural cultivation and affect the existence of agricultural arthropods. Different environmental conditions will influence the distribution patterns, richness and diversity of arthropods living in cultivated land. The research was conducted at 8 rice cultivation locations in Greater Malang. Observation of arthropods using pitfall and yellow pan traps, with a diagonal pattern. The total arthropods obtained were 3,937 individuals, 39 families, 46 genera and 48 species. The highest predator species found were Micraspis discolor, the herbivore Nilaparvata lugens, other insect is Culista incidens, and the predator is Drynus baegamensis. The highest index analysis for diversity in Lowokwaru H'= 3,075 in the high category, evenness in Karangploso E=0,864 in the high category and dominance in Pagelaran D=0,179 in the low category. PCA analysis of species composition divided into 2 habitat types, lowland and medium land groups. Species richness increased with increasing observation locations. Environmental factors will bring in species that are suitable for the habitat. Further research needs to be carried out regarding ecological variables or environmental factors, which affect the patterns composition and species richness of arthropods.
Effect of Landscape Composition and Habitat Conditions on the Diversity of Predatory Insects (Spiders, Ants, and Ground Beetles) in Cabbage Fields Syahputra, Arif firman; Rizali, Akhmad; Hadi, Mochamad Syamsul
Research Journal of Life Science Vol 11, No 1 (2024)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2024.011.01.1

Abstract

In a landscape, various types of land use compositions and different habitat conditions can affect the diversity and abundance of living organisms, particularly predatory insects. The observed landscape is a cabbage plantation area located in the highlands, featuring diverse habitat conditions, vegetation variations, and surrounding land use compositions such as agriculture, natural habitats, industrial areas, and settlements. This study aims to determine the diversity and abundance of predators, particularly spiders, ants, and ground beetles, in cabbage farming, influenced by differences in landscape composition and habitat conditions in the Malang and Pasuruan districts. Landscape analysis was conducted through direct checking (ground survey), followed by digitization using QGIS software, and data analysis was performed using the RStudio application. Plant age did not affect the diversity and abundance of predatory insects in cabbage plants. Elevation was related to the abundance of ants, as well as the diversity and abundance of ground beetles. The use of insecticides had no relationship with the diversity and abundance of predatory insects. Almost all land use compositions around the study area significantly impacted the diversity and abundance of predators. Therefore, it can be concluded that certain habitat conditions and landscape compositions are related to the diversity and abundance of predatory insects in cabbage plants.
Use Of Organic Fertilizer and IPM in Certified Organic Rice Farming in East Java Winarno, Dinariningrum Rahma; Tarno, Hagus; Mudjiono, Gatot
Research Journal of Life Science Vol 11, No 1 (2024)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2024.011.01.2

Abstract

This study aims to analyze the effect of organic fertilizer use and Integrated Pest Management (IPM) on the success of certified organic rice farming in East Java. The research method used is descriptive quantitative with Structural Equation Modeling-Partial Least Squares (SEM-PLS) analysis. Data was collected by surveying 100 organic rice farmers in Kediri District. The results showed that the use of organic fertilizers and IPM significantly increased the productivity of organic rice. Organic fertilizers, such as compost and animal manure, can improve soil fertility and crop yields. Meanwhile, IPM helps control pests without using chemical pesticides. The SEM-PLS model confirmed that both variables positively and significantly influence the success of organic rice farming. The results also show that farmers who implement organic farming have a higher level of environmental awareness and are more concerned about consumer health. However, some challenges are still faced, such as limited market access and farmers' lack of knowledge about organic farming techniques. Therefore, more intensive efforts are needed to provide education and training to farmers, as well as to develop policies that support the development of organic farming. In conclusion, the application of organic fertilizer and IPM is the key to the success of organic rice farming in East Java. With the right support, organic farming can be a sustainable solution to increase productivity, preserve the environment, and improve farmers' welfare.

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