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Journal of Tropical Biodiversity and Biotechnology
ISSN : 25409573     EISSN : 25409581     DOI : -
Journal of Tropical Biodiversity and Biotechnology (JTBB) is an authoritative source of information concerned with the advancement of tropical biology studies in the Southeast Asia Region. It publishes original scientific work related to a diverse range of fields in tropical biodiversity, functional biology, and biotechnology.
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Articles 327 Documents
Intraspecific Variability and Phenetic Relationships of Centella Asiatica (L.) Urb. Accessions from Central Java Based on Morphological Characters’ Maruzy, Anshary; Susandarini, Ratna
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.86477

Abstract

Centella asiatica (L.) Urb. is a plant species native to Java and one of the main basic materials in traditional and modern medicine. This study is the first to report the intraspecific variation and taxonomic relationships of C. asiatica accession from natural populations in Central Java.  The purpose of this study was to reveal phenotypic variations of C. asiatica populations and to asses phenetic relationships based on morphological characters. Thirty-two accessions of C. asiatica were collected from natural populations from eight mountains in Central Java. Observation on vegetative organs resulted in 25 morphological characters as a basis for assessing phenetic relationships using cluster analysis and principal component analysis. Result of cluster analysis showed that the grouping of accessions was not correlated to the localities from where the samples were collected, although there was a tendency that accessions from the same localities grouped in one cluster. The results of this study confirmed the existence of intraspecies morphological variability in C. asiatica which was not affected by geographical aspects. Results of principal component analysis indicated that the grouping of accessions was mainly determined by similarities in petiole color, stolon color, leaf margin, petiole length, stolon length, and leaf color. Given that the characters contributing to the grouping of accessions were mainly qualitative characters, the results indicated a genetic basis underlying phenotypic variations of C. asiatica accession.  
Morphological Variation of Diospyros spp. Native to Sulawesi Based on Vegetative Organ Characters Wulandari, Aprilia Ayu; Khotimperwati, Lilih; Wanda, Irvan Fadli
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 2 (2024): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.86597

Abstract

Researchers about Diospyros have not extensively conducted studies on the morphological characteristics to enhance information for taxonomic hierarchy purposes. Diospyros in Indonesia has great potential for in-depth research due to its unique characteristics, particularly in Sulawesi. This research aims to fill the knowledge gap regarding investigating phenotypic variations in Diospyros, specifically in the Sulawesi region. Nine species of Diospyros from the BBG collection will be observed and described for their morphological characteristics. The results indicate 25 variations of 50 characters in morphological characteristics. Variations in morphological characters can suggest that each species adapts to the surrounding environmental conditions.
Bioprospecting and Molecular Identification of Amylase and Cellulase Producing Thermophilic Bacteria from Sediment of Nglimut Hot Springs, Kendal Regency Budiharjo, Anto; Wulandari, Dyah; Shabrina, Jauhara; Mawarni, Risa Arum; Maulana, Anand Reyna; Nurhayati, Nurhayati; Wijanarka, Wijanarka; Hartajanie, Laksmi; Lindayani, Lindayani
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 3 (2024): September
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.86756

Abstract

The utilisation of enzymes in the industry has brought numerous benefits and advantages to production processes. Enzymes serve as biocatalysts, efficiently catalyzing reactions and hydrolysis in biochemical processes. However, there are challenges in applying enzymes in the industry, particularly concerning enzyme stability. The obstacle encountered in the production processes involving industrial enzyme applications is the low stability of enzymes when used at high temperatures. Heat-sensitive enzymes undergo damage or denaturation. Thermophilic microorganisms are chosen because they hold the potential to produce thermophilic enzymes. The thermophilic enzymes exhibit better heat stability compared to other enzymes, making them an effective alternative for future industrial production processes. This study aims to isolate thermotolerant bacteria from Nglimut Hot Spring sediment, screen for cellulase- and amylase-producing isolates, and molecularly identify the best isolate using 16S rRNA barcode. The results show that 22 bacterial isolates were found in the sediment of a hot spring; TS-14 was the best isolate in producing amylase, with the highest average amylolytic index of 2.38, whereas TS-15 had the highest cellulolytic index of 2.11. Based on 16S rRNA identification, TS-14 showed an homological identity of 79% with Bacillus amyloliquefaciens, while TS-15 had a 100% homological identity with Bacillus licheniformis. These results were important as the first step of screening bacterial potential to produce thermophilic enzymes that could be applied in the downstream processing in future industrial and biotechnology companies. 
First Report of Cladosporium dominicanum Zalar, de Hoog & Gunde-Cim. Infecting Whitefly on Ornamental Plants in Bali, Indonesia Sudiarta, I Putu; Sugiarta, Dwi; Selangga, Dewa Gede Wiryangga; Wirya, Gusti Ngurah Alit Susanta; Gargita, I Wayan Diksa; Yuliadhi, Ketut Ayu; Klett, Katrina
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 2 (2024): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.86865

Abstract

As natural enemies, entomopathogenic fungi are essential for controlling certain plant pests, such as whitefly. Many types of entomopathogenic fungi can infect whiteflies, including those from the genera Aschersonia, Metarhizium, Beauveria, and Cladosporium. Currently, there is great interest in using entomopathogenic fungi as an environmentally friendly pest control in organic and sustainable agricultural systems. This study aimed to identify entomopathogenic fungus associated with whiteflies. Identification of entomopathogenic fungus was performed morphologically and molecularly using DNA barcoding with ITS-1 and ITS-4 as primers, in addition to sequencing and phylogenetic tree analysis. Whiteflies infected with entomopathogenic fungus were discovered on ornamental plants (Premna serratifolia, Ficus religiosa, and Ficus rumphii) in Denpasar, Bali, Indonesia from January to March 2023. We found that samples from the field had mycelium that was unbranched or sparingly branched with solitary conidiophores arising terminally from ascending hyphae or laterally from plagiotropous hyphae. Samples from PDA media had ramoconidia with darkened septa, straight and conidia in long branched chains, branching in all directions, conidia were obovoid, ovoid to limoniform. The fungal colonies had the characteristics of being grey-olivaceous, olivaceous-black, and the margins were grey-livaceous to white, gray olivaceous in colony center due to abundant sporulation. In addition, based on the BLAST nucleotide sequence from NCBI GenBank, the insect pathogen phylogenetic tree found in Denpasar, Bali, is in the same clade as Cladosporium dominicanum strain SCAU014, accession number KY827344.1. Based on morphological and molecular analysis the fungus that infects whiteflies on ornamental plants is C. dominicanum.
Safety Assessment of Bacillus subtilis G8 Isolated from Natto for Food Application Putri, Nathania Calista; Victor, Hans; Litanto, Vivian; Pinontoan, Reinhard; Jo, Juandy
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.87079

Abstract

Various bacteria are widely used as food-fermenting agents, including Lactobacillus, Bifidobacterium, and Bacillus. Despite they are generally recognized as safe to be consumed by humans, those bacteria could potentially cause antibiotic resistance as they could acquire and transfer antibiotic resistance genes from or to other microbes within the human gastrointestinal tract. Profiling antibiotic resistance pattern in those bacteria is therefore important to control the spread of antibiotic resistance. In this study, antibiotic resistance profile of Bacillus subtilis G8 was assessed. B. subtilis G8 had been isolated from commercialised Japanese natto in Indonesia and had been previously reported for its fibrinolytic characteristics. The antibiotic resistance phenotype and genotype of B. subtilis G8 were assessed through the Kirby-Bauer disk diffusion method and whole-genome analysis, respectively. B. subtilis G8 exhibited resistance towards Oxacillin, Lincomycin and Tiamulin-Lefamulin. The bioinformatics analysis indicated several responsible genes mediating those resistance, i.e., ybxI (for Oxacillin), lmrB (for Lincomycin) and vmlR (for Lincomycin and Tiamulin-Lefamulin). All identified genes were found in the chromosomal DNA. Further analysis found no mobile genetic elements within the genome, therefore reducing a risk of resistance gene transfer via plasmid and subsequently supporting safety profile of B. subtilis G8 in food fermentation usage.
Basidiomycota Macrofungal Communities Across Four Altitudinal Ranges in Bukit Baka Bukit Raya National Park, Indonesia Harun, Natasya Adelia; Lovadi, Irwan; Rahmawati, Rahmawati; Joharudin, Didin
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.87309

Abstract

The influence of elevation gradient has been investigated across different taxa. However, such studies are scarce for macrofungal communities. This study examined the community structure of Basidiomycota macrofungi across four elevations in Bukit Baka Bukit Raya National Park, Indonesia. Macrofungi were collected from randomly placed five 10 x 10 m plots at each altitude and identified at the genus level. The results showed that there were 32 genera belonging to 20 families. The NMDS ordination and ANOSIM confirmed that macrofungal composition and abundance do not differ between the studied altitudinal ranges. 
Composition and Conservation Status of Avifauna in Urban Non-protected Important Bird Area (IBA) Site of Western India Makwana, Vishal M.; Khatsuriya, Prakruti A.; Matli, Aamir G.; Dodia, Pravinsang P.
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 2 (2024): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.87311

Abstract

Kumbharwada wetland, an Important Bird Area (IBA) component in Bhavnagar, Gujarat, India, is a crucial wintering ground for migratory birds. Long-term monitoring of the avian community over a period of long time-frame is an excellent way to examine the health of this IBA site and thus provide an important ground to foster the conservation of birds in the region and management of this wetland. Given this consideration, field surveys were carried out from December 2020 to May 2023, following point count method to study the avian species richness in the study area. A total of 204 bird species belonging to 20 orders and 56 families are recorded, of which 85 species are migratory and 119 are resident. Anatidae is the most species-rich avian family (16 species). Highest number of species was recorded in the month of January (165 species in 2021 and 163 species in 2022). This wetland supports 107 (52.45%) wetland-associated species and 97 (47.55%) terrestrial species of birds. Twelve species are considered as Near Threatened, four species as Vulnerable and one species (Aquila nipalensis) as Endangered in IUCN Red List of Threatened Species. Four species (Sterna aurantia, Mycteria leucocephala, Phoeniconaias minor, and Threskiornis melanocephalus) with globally declining trend, are commonly seen in the study area, which shows that the wetland is a crucial habitat for bird species with high conservation priorities. Industrialization, encroachment, discharge of sewage water and chemical effluents, high-tension powerlines, stray dogs, and expansion of exotic vegetation remained the major threats to the habitat and avian community. 
Occurrence of Cassava Lace Bug Vatiga illudens (Drake, 1922) (Hemiptera: Heteroptera: Tingidae) in Bali, Indonesia Sudiarta, I Putu; Dinarkaya, Shah Mahapati; Devi, Komang Saraswati; Ariyanta, I Putu Bawa; Wirya, Gusti Ngurah Alit Susanta; Sugiarta, Dwi; Selangga, Dewa Gede Wiryangga; Gargita, I Wayan Diksa; Wiguna, Putu Perdana Kusuma; Yuliadhi, Ketut Ayu; Devi, Putu Shinta
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.87438

Abstract

Cassava Lace Bugs (CLB) are native pest of cassava (Manihot esculenta Crantz, Euphorbiaceae) to the Neotropical Region, mainly in Brazil. On the other hand, East Java was the first region in Indonesia to record the presence of CLB in 2021, however, it has not been reported in other regions in Indonesia. Therefore, the very importance to recognise the occurrence of CLB in other regions in Indonesia. Based on this, the research has been carried out starting with a field survey, observing behaviour of insect in the field and identify morphologically in the laboratory. The survey results show that the infestation of CLB has been found in lowland, medium, and highland areas in Bali. Symptoms of infestation on the upper leaf surface are small yellow spots with brownish variations. Based on the identification key, CLB from Bali Indonesia, shows a characteristic of a head with a pair of frontal spines. Based on this evidence, the CLB insect can be identified as Vatiga illudens (Hemiptera: Heteroptera: Tingidae). It is the first report of novel distribution areas for V. illudens in Bali, Indonesia. The results of this research are important because V. illudens is one of the main pests of cassava. 
In Vitro Seed Germination and Shoot Growth of Nepenthes jamban Chi. C. Lee, Hernawati & Akhriadi, A Unique Pitcher Plant from Indonesia Prawestri, Apriliana Dyah; Rahayu, Resa Sri; Kurniajati, Wulan Septiningtyas; Sunardi, Sunardi; Mansur, Muhammad
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 2 (2024): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.87674

Abstract

The study to optimize in vitro propagation of the Indonesian native and critically endangered species, Nepenthes jamban, in order to support the ex-situ conservation efforts has been done.  Using Murashige and Skoog (MS) as a basal media, disinfected seeds of N. jamban were germinated on five types of germination media, viz. ¼ MS, ½ MS, MS, ¼ MS+benzyl adenine (BA)+Biotin and MS+BA+Biotin. Afterwards, in vitro shoots with 6-7 leaves were inoculated on growing media, i.e., ¼ MS, ¼ MS 60 (3:1) (MS modification with a higher concentration of nitrogen), and ¼ MS+naphtalene acetic acid (NAA)+BA. The results showed that the germination of N. jamban seeds was slow, indicated by the percentage of germination being less than 20% after 6 months of being planted on germination media. The highest percentage of germination was after the 6th month and the greatest pitcher development at the 10th month were obtained on ¼ MS medium. Furthermore, shoot growth and pitchers development consistently increased for 12 months in ¼ MS 60 (3:1) medium while other media resulted in a decrease in pitcher formation. It seemed that low concentrations of nutrient in the medium proved to be more effective to induce in vitro seed germination and enhance shoot growth which was also supported by higher nitrogen (nitrate) concentration in the medium. This study provides information that supports ex situ conservation action of native and critically endangered Nepenthes species from Indonesia. 
Fern Species-Area Relationship in Urban Anthropogenic Islands in Slawi, Tegal, Central Java Sedayu, Agung; Putri, Novita; Aminudin, Aminudin; Mawardi, Muchtar; Noer, M. Isnin; Maulana, Lana
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 3 (2024): September
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.87781

Abstract

In anthropogenic islands as urban parks, the fern species richness and composition may be determined ecologically by the quality of habitat, including area greenness, or biogeographically by area size. As the development of the theory of island biogeography also includes man-made parks, it is feasible to test whether area-species relationship applies in these urban parks, and is more pronouncedly evident compared to another ecological factor, such as NDVI. Total species number and composition of 8 urban parks in a kecamatan in Tegal Regency were collected and arranged in clustering methods to understand the similarity between parks. The similarity analysis result is important for the management of the parks in Slawi. The species richness data is subsequently tested using Pearson correlation and regression against NDVI and area sizes. The relation between NDVI and species richness is non-significant (p=0.058), while area size and species richness is significant (p=0.003). This signifies that the urban fern species richness is determined by area as biogeographical factor, compared to NDVI as ecological factor. This result is important for the purpose of designing and managing urban parks as evidently size is important in the effort of attracting native biodiversity into urban parks and in turn enhancing the well-being of urban population.