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INDONESIA
METAMORFOSA Journal of Biological Sciences
Published by Universitas Udayana
ISSN : 23025697     EISSN : 26558122     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
METAMORFOSA is an electronic scientific journal published periodically by the Master of Biology Udayana University, which includes scientific works in the field of Biology.
Arjuna Subject : -
Articles 316 Documents
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Struktur Komunitas Fitoplankton di Area Keramba Jaring Apung Danau Buyan Kecamatan Sukasada, Buleleng, Propinsi Bali Putu Roni Graha Persada; I Wayan Restu; Alfi Hermawati Waskita Sari
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p03

Abstract

Danau Buyan merupakan sumberdaya alam akuatik yang mempunyai nilai yang sangat penting. Pemanfaatan danau buyan diantaranya adalah kegiatan budidaya keramba jaring apung yang berpotensi memberikan masukan nutrien kondisi ini dapat mempengaruhi kualitas air yang kemudian akan berpengaruh pada fitoplankton. Tujuan penelitian ini adalah untuk mengetahui struktur komunitas fitoplankton dan kualitas perairan di area keramba jaring apung Danau Buyan, Kecamatan Sukasada, Propinsi Bali. Penelitian ini dilaksanakan pada bulan Januari-Maret 2016 di area keramba jaring apung Danau Buyan, Kecamatan Sukasada, Kabupaten Buleleng, Propinsi Bali. Penelitian ini menggunakan metode sistematic sampling. Lokasi stasiun pengamatan total berjumlah 8 stasiun, stasiun 1-7 terletak dekat dengan area budidaya keramba jaring apung sedangkan 1 stasiun kontrol berada jauh dari area keramba jaring apung. Kegiatan pengambilan sampel dilakukan 3 kali dengan selang waktu 3 minggu. Hasil penelitian menunjukkan terdapat 9 genus fitoplankton yang terdiri dari 24 spesies. Hasil kelimpahan fitoplankton rata-rata yaitu 1454 sel/l sehingga masuk dalam kategori perairan oligotropik, Indeks keanekaragaman 07647-1,8352, Indeks keseragaman rata-rata-rata 3937. Nilai indeks dominansi 0,4225. Nilai parameter kualitas air yang didapatkan antara lain yaitu nilai suhu sebesar 23,54-24,94oC, Nilai Kecerahan yaitu 66,41-251.06, Nilai kekeruhan yaitu sebesar 2,10-6,39 NTU, Nilai pH yaitu sebesar 7,56-8,93, Nilai oksigen terlarut (DO) yaitu sebesar 4,10-5,44 mg/l, Nilai nitrat yaitu sebesar 0,39-0,99 mg/l dan nilai fosfat yaitu sebesar 0,13-0,45 mg/l. Secara keseluruhan hanya parameter oksigen terlarut (DO) yang kurang mendukung kehidupan fitoplankton.
THE EFFECT OF MAXIMUM PHYSICAL ACTIVITY AND MANGGONG BAMBOO (Gigantochloa manggong) LEAF EXTRACT ON CATALASE ACTIVITY IN LIVER ORGAN OF RATS (Rattus norvegicus) Supriyatin Supriyatin; Sri Rahayu; Ririn Apriana
Metamorfosa: Journal of Biological Sciences Vol 4 No 1 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i01.p02

Abstract

Gigantochloa manggong, one of endemic bamboo plant in Indonesia is suspected to have exogenous antioxidant potential. Exogenous antioxidant can help the activity of endogenous antioxidant in the body when overtraining occurs. Antioxidant can be measured by catalase enzymes activity. This study was carried out to determine the effect of maximum physical activity and leaf bamboo extract on catalase activity in liver organ of rats. This research used experimental method with completely randomized design (CRD). The test groups were the control rats (E0R0), leaf extract induced rats (E1R0), swimming activity treated rats (E0R1) and leaf extract induced and swimming activity treated rats (E1R1). Data were analyzed by the two-way ANOVA statistical test. Bamboo leaf extract non-toxic and leaf extract contained flavonoids, alkaloids, triterpenoids dan saponins. Catalase units in the control group is 1.00 unit/ml, the induced leaf extract group is 0.89 unit/ml, the maximum physical activity group is 0.78 unit/ml and the maximum physical activity treated and induced leaf extract group is 0.56 unit/ml. Based on statistical test, catalase activity has no effect (p>0,05) on rats. It was concluded that maximum physical activity could not reduce catalase activity. Manggong bamboo leaves extract could not increase catalase activity and there was no effect between maximum physical activity and manggong bamboo leaf extract on catalase activity in liver organ of rats.
Bioetanol dari Kulit Pisang (Musa paradisiaca L.) dengan Sakarifikasi dan Fermentasi Serentak I Ketut Muksin; Ni Luh Arpiwi
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p17

Abstract

Bioethanol is one of the energy alternatives which environmentally friendly and sustainable. This research employed Completely Randomized Design with Factorial. There were two treatments, namely without pretreatment and with pretreatment and these were factorized with three levels of urea, namely 0, 1 and 2% with three replications. Finely dry ground banana peel samples were pretreated with NaOH 6% w/v. Simultaneous saccharification and fermentation was performed in 60 mL fermenter. Sample (4% w/v) was placed in a fermenter and the following ingredients were added: 0.06 g KH2PO4, 0.06 g MgSO4 and citrate buffer with pH 5. Fermenter was enclosed and autoclaved at 121oC for 15 minutes and then cooled down at room temperature. Enzyme cellulose (6% w/v) and inoculum of Saccharomyces cerevisiae (10% v/v) were added into the fermenter. The mixture was incubated at 30oC for 9 days, and every day 3rd, 5th, 7th, and 9th was measured for reducing sugar content, pH and bioethanol content. Bioethanol content of the distillate was measured using picnometer. Data was analyzed using ANOVA (Analysis of Variance) with Minitab 17 software. If there was significant (P<0.05) analysis continued with Tukey Pairwise comparison to find the difference among treatments. Results showed that treatment (without pretreatment and with pretreatment of NaOH 6% b/v) combined with levels of urea gave significant effect on reducing sugar and bioethanol content in all observations. Combination of without pretreatment and urea level at 1% and 2% resulted in the highest bioethanol content of 4,91% v/v.Bioethanol is one of the energy alternatives which environmentally friendly and sustainable. This research employed Completely Randomized Design with Factorial. There were two treatments, namely without pretreatment and with pretreatment and these were factorized with three levels of urea, namely 0, 1 and 2% with three replications. Finely dry ground banana peel samples were pretreated with NaOH 6% w/v. Simultaneous saccharification and fermentation was performed in 60 mL fermenter. Sample (4% w/v) was placed in a fermenter and the following ingredients were added: 0.06 g KH2PO4, 0.06 g MgSO4 and citrate buffer with pH 5. Fermenter was enclosed and autoclaved at 121oC for 15 minutes and then cooled down at room temperature. Enzyme cellulose (6% w/v) and inoculum of Saccharomyces cerevisiae (10% v/v) were added into the fermenter. The mixture was incubated at 30oC for 9 days, and every day 3rd, 5th, 7th, and 9th was measured for reducing sugar content, pH and bioethanol content. Bioethanol content of the distillate was measured using picnometer. Data was analyzed using ANOVA (Analysis of Variance) with Minitab 17 software. If there was significant (P<0.05) analysis continued with Tukey Pairwise comparison to find the difference among treatments. Results showed that treatment (without pretreatment and with pretreatment of NaOH 6% b/v) combined with levels of urea gave significant effect on reducing sugar and bioethanol content in all observations. Combination of without pretreatment and urea level at 1% and 2% resulted in the highest bioethanol content of 4,91% v/v.
IDENTIFIKASI ANTAGONIS DARI Xanthomonas campestris YANG DIISOLASI DARI RHIZOSPHERE PERKEBUNAN BROKOLI (Brassica oleracea var. italica) DI DESA KEMBANG MERTA, KABUPATEN TABANAN, BALI Nadya Treesna Wulansari; Yan Ramona; Meitini Wahyuni Proborini
Metamorfosa: Journal of Biological Sciences Vol 2 No 1 (2015)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The main objectives of this research were to isolate and identify antagonists of Xanthomonas campestris from rhizosphere zone of broccoli plants. Soil samples were collected from broccoli farm located at Kembang Merta village, Tabanan, Bali. Isolation and identification of the antagonists were conducted at the Laboratory of Microbiology, Udayana University. Two fungal (Trichoderma harzianum and  Trichoderma viride) and two bacterial (Bacillus sp. and Pseudomonas sp.) antagonists potentially to be developed as biocontrol agents of Xanthomonas campestris were successfully identified in this research.
Dinamika Populasi Cendana (Santalum album L.) Di Hutan Dan Kebun Di Pulau Timor Barat, Nusa Tenggara Timur-Indonesia Yoseph Nahak Seran; Sudarto Sudarto; Luchman Hakim; Endang Arisoesilaningsih
Metamorfosa: Journal of Biological Sciences Vol 7 No 1 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i01.p12

Abstract

Sandalwood (Santalum album L.) is a endemic spesies of the Lesser Sunda Island (NTT), grows in deciduous forest or in private traditional plantation for centuries. Study aimed to evaluated profile dynamic structure population of sandalwood in forest and plantation in the district of South Central Timor (TTS) and North Central Timor (TTU). Population structure analysis was held by purposive sampling in eight sites comprised 87 plots. Plots size were 20x20 m2 (trees), 10x10 m2 (poles), 5x5 m2 (saplings), 2x2 m2 (seedlings). Variables observed in each plot were included density, stem diameter, height of free trunk (TBBC), height trees and saplings, crown quality and coverage. Data were analyzed by qualitative descriptively and multivariate statistics. Results showed that population structure of sandalwood varied spatially in all study sites in two district, especially density of seedlings and saplings in plantations and forests. Sandalwood trees density ranges from 0-23 ind.ha-1, poles phase 80-322 ind.ha-1, saplings phase 60-1,289 ind.ha-1, while the seedlings phase is very dominant 2,000-27,813 ind.ha-1. Higher saplings and poles density were found in two sites of TTU plantation and forest or a TTS forest. Farmer in two districts successfully cultivated sandalwood in plantation shown by similar growth quality with those of forest trees. Keywords: Forest, NTT, population structure, plantation, sandalwood
TINGKAT KESUBURAN PERAIRAN BERDASARKAN KELIMPAHAN FITOPLANKTON DI DANAU BATUR, KINTAMANI, BALI I Gusti Ayu Novie Sidaningrat; I Wayan Arthana; Endang Wulandari Suryaningtyas
Metamorfosa: Journal of Biological Sciences Vol 5 No 1 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i01.p12

Abstract

Danau Batur merupakan danau terbesar di Bali yang terletak di daerah Kintamani, Kabupaten Bangli. Danau Batur berada pada ketinggian 1.050 meter di atas permukaan laut (dpl) yang secara geografis terletak pada posisi 115022’42,3”-115025’33,0” Bujur Timur dan 8013’24,0”-8017’13,3” Lintang Selatan. Permukaan air Danau Batur memiliki luas yaitu 16,05 km2, dengan volume air 815,38 juta m3 dan kedalaman rata-rata 50,8 m. Luas daerah tangkapan 105,35 km2, selain itu kegiatan lain yang dilakukan di sekitar Danau Batur adalah kegiatan pertanian (49,35%), budidaya keramba jaring apung, pemukiman penduduk (2,22%), kegiatan pariwisata, dan dermaga. Kegiatan tersebut dapat menambah masukan unsur hara ke perairan yang berpengaruh terhadap kesuburan perairan. Tujuan dari penelitian ini adalah untuk mengetahui kelimpahan fitoplankton yang dapat dijadikan sebagai indikator kesuburan perairan Danau Batur. Hasil penelitian komunitas plankton didukung dengan keanekaragaman jenis fitoplankton sebanyak 16 spesies dari 4 filum. Nilai kelimpahan fitoplankton berkisar antara 2.686-2.983 individu/Liter yang termasuk ke dalam perairan mesotrofik (tingkat kesuburan perairan sedang). Hasil pengukuran parameter kualitas air secara berturut-turut dengan kisaran nilai suhu 24,9-25,60C; pH 9,02-9,06; DO 4,4-5,0 mg/L; kecerahan 119,1-138,4 cm; nitrat 0,452-0,598 mg/L; dan fosfat 0,33 -0,488 mg/L.
ANALISIS BAKTERIOLOGI SAMPLE MINUMAN YANG DIAMBIL DARI AREA SEKITAR KAMPUS II SEKOLAH TINGGI ILMU KESEHATAN BALI Nadya Treesna Wulansari; Juli Marjati; Luh Ami Yuliantika; Ida Ayu Suptika Strisanti
Metamorfosa: Journal of Biological Sciences Vol 4 No 2 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i02.p14

Abstract

Penelitian ini bertujuan untuk mengetahui kualitas dan kuantitas bakteriologi pada minuman di beberapa tempat makan sekitar kampus II STIKES Bali. Pengambilan sampel menggunakan metode purposive sampling. Sampel minuman diambil dari beberapa tempat makan di sekitar lingkungan Kampus II STIKES Bali. Analisis bakteriologi minuman dilakukan dengan menggunakan metode MPN (Most Probable Number) Coliform di UPT. Balai Laboratorium Kesehatan Provinsi Bali. Hasil penelitian ini menunjukkan bahwa 27 sampel minuman (90%) terkontaminasi bakteri coliform dengan rentangan jumlah 2 - 240 Coliform/100 mL dan 3 sampel minuman (10%) tidak mengandung bakteri coliform. Sedangkan dari 27 sampel minuman yang mengandung Coliform, menunjukkan bahwa 23 sampel minuman (85,19%) terkontaminasi bakteri Fecal coli dan 4 sampel minuman (14,81%) tidak mengandung bakteri Fecal coli.
Keragaman Genetik dan Hubungan Kekerabatan Ayam Lokal Berdasarkan Penanda RAPD Mery Wintari; Made Pharmawati; Ngurah Intan Wiratmini
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p13

Abstract

Indonesian local chicken is one kind of germplasm that has been utilized by the community for various needs, such as consumption, ceremonial, collection, and cockfighting. To maintain its sustainability, an inventory of genetic diversity can be made using molecular markers. This study aimed to determine the genetic diversity and relationship between four Indonesian local chickens: ayam kampung, ayam ketawa, ayam kate lokal, and ayam bekisar kangean, based on RAPD marker. DNA was extracted from blood samples of 12 local chickens consisting of 3 ayam kampung, 3 ayam ketawa, 3 ayam kate lokal, and 3 ayam bekisar kangean using Genetic DNA Mini Kit (Tissue) Geneaid. The PCR-RAPD analysis was performed using 6 primers, OPA 04, OPB 08, OPB 12, OPC 16, OPH 01, and OPH 03. The product of PCR-RAPD was electrophoresed using 1,5% agarose in 1x TAE buffer. The results showed that the similarity value of 12 local chickens using RAPD ranged from 0% to 100%. The relationship between 12 local chickens using RAPD marker indicated that the chickens in the same morphology were not clustered in the same group. This suggests that the same group of chickens phenotypically does not necessarily have the same genotype. Keywords: Local Chicken, PCR-RAPD
Pemilihan Pakan Alami Untuk Mendukung Pemeliharaan Ikan Capungan Banggai (Pterapogon kauderni) Di Lini Aquaculture Training Center (Latc) Bali Hasrat Damai Hulu; Pande Gde Sasmita Julyantoro; I Wayan Kasa
Metamorfosa: Journal of Biological Sciences Vol 7 No 1 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i01.p01

Abstract

This research was aimed to obtain the kind of proper natural food and the optimum dose of feeding in order to support the rearing of banggai cardinal fish breeding. This research used completely randomized design and was conducted during September to December 2016. The kinds of natural food examined were trash fish, abalone (Haliotis glabra), and squid (Loligo pealei). After we found the most preferable food, the examination upon its dose was conducted under 10%, 20% and 30% of the fish weight. The 45 fish sampel have average length and weight of 3 cm and 0,47 g, respectively. Fish were reared in aquarium 95 cm x 45 cm x 35 cm. The foods were given three times a day. The research results show that abalone was mostly consumed compared to other two foods about 0,11 g/fish a day. The result of examining dose showed that the highest rate of fish growth was found under the dose of 30% which resulted 2,07 cm in length, 0,34 g in weight and the dose of 20% which resulted 2,07 cm in length, 0,33 g in weight. The statistical test result showed that the treatment under the dose of 30% and 20% was not significantly different (P>0,05). Base on this study, it can be concluded that abalone is the proper natural food that supports the rearing of banggai cardinal fish with optimum dose of 20 % of fish weight per day. Keywords: Banggai cardinal fish, Natural Feed, Lini Aquaculture Training Center.
STUDI HISTOPATOLOGI INSANG IKAN NILA (Oreochromis niloticus, Linn.) DITINJAU DARI KADAR AMMONIA (NH3) DI DANAU BATUR, BALI Desak Wira Triana Wandari; I Wayan Restu; Endang Wulandari Suryaningtyas
Metamorfosa: Journal of Biological Sciences Vol 5 No 1 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i01.p01

Abstract

Danau Batur merupakan danau terbesar di Pulau Bali yang berada di Kecamatan Kintamani, Kabupaten Bangli. Insang ikan Nila digunakan sebagai indikator karena merupakan organ yang sangat sensitif terhadap perubahan kualitas air. Penelitian bertujuan untuk mengetahui kondisi lingkungan perairan dan gambaran histopatologi insang Ikan Nila ditinjau dari kadar Ammonia (NH3) di Danau Batur. Penelitian dilaksanakan pada bulan Januari hingga Maret 2016 dengan metode yang digunakan adalah observasi langsung dan purposive sampling pada 5 Stasiun di Danau Batur. Analisis histopatologi dilakukan di laboratorium histopatologi, Balai Karantina Ikan dan pengendalian Mutu kelas 1 Denpasar, Bali. Sampel histopatologi insang dianalisa melalui proses isolasi, fiksasi, dehidrasi dan pembenaman pada paraffin dan pewarnaan dengan Haematoxylin-Eosin. Hasil kualitas air menujukkan nilai pH 8,71-9,08, nilai suhu 25,4-30,0°C, kadar DO 3,8-5,1 mg/L yang masih sesuai pada ambang baku mutu kelas III untuk kegiatan perikanan, sedangkan kadar NH3 0,027-0,105 mg/L berada di atas nilai ambang baku mutu air kelas III untuk kegiatan perikanan mengacu pada PP Nomor 81 Tahun 2001 tentang Pengolahan Kualitas Air dan Pencemaran Air. Hasil pengamatan histopatologi memperlihatkan adanya perubahan yang ditinjau dari kadar NH3. Perubahan yang terjadi pada insang berupa fusi, hiperplasia, apoptosis, telengiektasis dan haemoragii.

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