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METAMORFOSA Journal of Biological Sciences
Published by Universitas Udayana
ISSN : 23025697     EISSN : 26558122     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
METAMORFOSA is an electronic scientific journal published periodically by the Master of Biology Udayana University, which includes scientific works in the field of Biology.
Arjuna Subject : -
Articles 316 Documents
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PENGARUH PERTUMBUHAN MISELIUM BEBERAPA JENIS JAMUR TIRAM (Pleourotus spp.) MELALUI PENAMBAHAN KALSIT : DOLOMIT DALAM MEDIA SERBUK GERGAJI Hafizatur Rahma; Nurmiati Nurmiati; Deddi Prima Putra; Periadnadi Periadnadi
Metamorfosa: Journal of Biological Sciences Vol 4 No 2 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i02.p01

Abstract

Penelitian tentang “Pengaruh pertumbuhan miselium beberapa jenis jamur tiram (Pleourotus spp.) melalui penambahan kalsit : dolomit” dilakukan untuk mengetahui pengaruh penambahan kalsit : dolomit terhadap lama pertumbuhan miselium (vegetatif) beberapa jenis jamur tiram (Pleourotus spp.). Penelitian ini dilakukan di Laboratorium Mikologi, Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas. Penelitian dilakukan dalam Rancangan Acak Lengkap (RAL) pola nested dengan 2 kali ulangan. Faktor A berupa jenis jamur tiram (P. ostreatus, P. sajor-caju, P. flabellatus, P.cystidiosus) dan faktor B berupa variasi penambahan kalsit : dolomit (kontrol, 1:0, 1:2, 1:1, 1:2, 0:1). Hasil penelitian menunjukkan bahwa pertumbuhan miselium tercepat diperoleh dengan penambahan kalsit : dolomit 1:1 (19,62 hari) oleh P. flabellatus. Secara keseluruhan pemberian kalsit : dolomit dalam media serbuk gergaji berpengaruh terhadap kecepatan pertumbuhan miselium jamur tiram (Pleurotus spp.).
Perubahan Golongan Darah Berdasarkan Pengaruh Waktu dan Mikroorganisme Yang Berperan Muhamad Masyrur; I Ketut Junitha; Meitini W. Proborini
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p05

Abstract

Blood is one of the most important evidence in the forensic analysis. Blood has a special characteristic or identity of the owner. Blood can be analyzed blood type to know the owner of the blood. But in the course of time, the blood will be degraded so it will be change of blood type into blood type O. One of the contributing factors is the presence of microorganisms that it grow in the blood. The material of this research is the human blood of blood type A, B, AB and O from 4 (four) respondents of 3 (three) males and 1 (one) female who are 20 to 40 years old. Each sampel of 150 µL was dropped on sterile gauze size 2 x 6 cm, then it was dried. Sample were stored at room temperature (± 30 oC) for 0, 30, 60, 90 and 120 days. Sampel was tested for blood type using absorption elution method based on the work instructions from The Police Forensic Laboratory of Denpasar Branch in 2016. The results of this research indicate that the human dried blood stored on sterile gauze media for 30 and 60 days has not changed its blood type. Storage of the human dried blood for 90 and 120 days, it have changed blood type with found antigen A or B that was not detected back through absorbtion elution analysis on blood group A, B and AB. This research found 9 species of fungi and 7 species of bacteria that they grow on the blood samples. Species of Fungi and bacteria that have high ability to degrading all blood group (A, B and AB) are Acromonium carticola, Alternaria alternata, Aspergillus flavus, Aspergillus niger, Penicillium sp and Bacillus coagulans. While species of fungi and bacteria that have high ability to degrading blood group B or AB are Fusarium sp, Trichoderma harzianum, Trichoderma viridae, Bacillus sp and Staphylococcus cohnii.
STRUKTUR KOMUNITAS IKAN PADA LUBUK LARANGAN DI DESA RANTAU PANDAN KABUPATEN BUNGO, JAMBI Diana Sari; Indra Junaidi Zakaria; Wilson Novarino
Metamorfosa: Journal of Biological Sciences Vol 3 No 2 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i02.p04

Abstract

The objective of this research is analyse variety of fish species in Lubuk Larangan, Rantau Pandan Village. Reseacher take two location for this research. First location has not inhabitant activity. It is Lubuk Larangan Sar (Headwaters). Meanwhile, at the second location is Lubuk Larangan Tepian (Lower Course). It is has inhabitant activity. This research conducted on March - August 2015 at waterworks Rantau Pandan Village, Bungo Regency, especially Jambi Province. The method was used in this research is survey, and used purposive sampling as technic to take sample based on river condition which is different as long as drainage area at Rantau Pandan Village. Fish species variety at Lubuk Larangan, Rantau Pandan Village is focus to the first location, and at the second location is not different (almost similar).
Perilaku Kawin Tikus (Rattus norvegicus) Jantan Diabetes Yang Diberi Ekstrak Etanol Kulit Buah Jengkol (Archidendron pauciflorum) Desak Made Malini; Emay Maulani; Sri Wulandari; Nining Ratningsih
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p17

Abstract

Diabetes mellitus (DM) may lead to disruption of normal sexual function in males including abnormalities of orgasmic/ejaculatory function and desire/libido. Simplisia of dried Jengkol fruit peel has been used by people to treat diabetes. The aim of this study was to examine the effectiveness of ethanol extract of jengkol fruit peel (A. pauciflorum) (EEJFP) in improving the sexual behavior of male rats (R. norvegicus) diabetic models. Induction of diabetes was carried out intravenously by using streptozotocin dose 60 mg/ kg BW in the test animals except for the negative control group.The treatments are negative control (Carboxy Methyl Cellulose (CMC) 0.05%), positive control (CMC 0.05%), P1 (EEJFP 385 mg/kg BW), P2 (EEJFP 770 mg/kg BW) and comparison (glibenclamide dose 10 mg/kg BW) for 54 days. The parameters observed are the number of introduction and climbing of male rats to female rats. Data obtained from each parameter were analyzed by analysis of variance (ANAVA) test with a 95% confidence level and Duncan's test. The results of this study indicate that the number of introductions and climbing in the P1 and P2 test animal groups are significantly different from the positive control treatment and close to the value in the negative control treatment group. It can be concluded that the ethanol extract of jengkol fruit peel (A. pauciflorum) affects the sexual behavior of male rats (R. norvegicus) diabetes models by increasing introduction and climbing. Keyword: diabetic, sexual behavior, Archidendron Pauciflorum, rats
Hambatan in Vitro Cendawan Antagonis pada Fusarium sp., Penyebab Penyakit pada Tanaman Buah Naga (Hylocereus undatus (Haw.) Britton & Rose) Dewa Ayu Andriastini; Yan Ramona; Meitini Wahyuni Proborini
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p14

Abstract

A research on in vitro inhibition of fungal antagonists, isolated from dragon fruit plantation in Sembung village, Bali, on Fusarium sp. (the disease causative agent of dragon fruit plant) was conducted with the main objective to investigate the effectiveness of these fungal antagonists to inhibit the in vitro growth of the pathogen. Dual assay method was applied in this experiment. The results showed that three potential fungal antagonists were successfully isolated in this research and they were identified as Trichoderma harzianum, Aspergillus niger, dan Paecilomyces lilacinus. All these fungal antagonists showed antagonistic activity against Fusarium sp. which was statistically significant (p<0.05) when compared to control. This indicated that all antagonist isolates were potential to be developed as biocontrol agent candidates.
IDENTIFIKASI SENYAWA GOLONGAN FENOL DARI EKSTRAK ETANOL DAUN SIRIH HIJAU (PIPER BETLE LINN.) DENGAN METODE KLT-SPEKTROFOTODENSITOMETRI Ni Made Pitri Susanti; Luh Putu Mirah Kusuma Dewi; Harlina Setiawati Manurung; I Made Agus Gelgel Wirasuta
Metamorfosa: Journal of Biological Sciences Vol 4 No 1 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i01.p16

Abstract

Phenol compound in Piper betle leaves has several pharmacology activities such as antibacteria, antifungi and antioxidant. The pharmacology activities of a herbal drug are influenced by the phytochemistry content, so in order to do a quality determination that provides phytochemistry profile and consistent pharmacology activities, a standardization is required. Fingerprint is the main standard to perform quality control for herbal drug. TLC-spectrophotodensitometry was used as the method in order to provide fingerprint profile of phenol compound. In this experiment, Piper betle leaves samples were extracted by reflux method using ethanol 96% as the solvent. Identification of phenol compound was done using TLC-spectrophotodensitometry with Silica gel 60 F254 as the stationary phase, toluena: ethyl acetate (93:7 v/v) as the mobile phase, FeCl3 and Folin-Ciocalteau as the reagent. The fingerprint profile of phenol compund was shown in Rf value 0,19; 0,42; and 0,62. Positive results of phenol compound are black spot on FeCl3 colour test and dark blue spot  on Folin-Ciocalteau colour test. Maximum wavelength of phenol compound was 283 nm.
Pengaruh pH dan Suhu terhadap Produksi Antibiotika dari Isolat Bakteri Endofitik pada Tumbuhan Andalas (Morus macroura Miq.) Riski Budi Yani; Anthoni Agustien; Feskaharny Alamsjah
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p14

Abstract

The study used survey method and the data were analysed descriptively. The selection of the bacteria which produce antibiotic had been with paper disk method and used Escherichia coli and Staphylococcus aureus as the sample bacteria. This result showed pH 7,0 was succesful optimum pH antibiotic produced for Bacillus sp.1 and Bacillus sp.2. and 370C was the optimum temperature to antibiotic produced from Bacillus sp.1 and Bacillus sp.2.
STRUKTUR KERAPATAN VEGETASI DAN ESTIMASI KANDUNGAN KARBON PADA BEBERAPA KONDISI HUTAN DI PULAU SIBERUT SUMATERA BARAT . Chairul; Erizal Muchktar; . Mansyurdin; Tesri Maideliza; Gusmardi Indra
Metamorfosa: Journal of Biological Sciences Vol 3 No 1 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i01.p03

Abstract

Hutan Tropik memiliki sumber cadangan karbon sangat besar yang dapat berubah akibat perubahan kondisi hutan yang disebabkan oleh aktivitas  manusia, termasuk penebangan dan perkebunan sehingga mengancam perubahan iklim dunia. Kajian analisis dilakukan untuk menetahu struktur vegetasi dan kandungan karbon pada tiga kondisi hutan di Pulau Siberut sebagai upaya mitigasi perubahan iklim. Untuk mendapatkan data biomassa bagian atas tanah dan serasah digunakan metode plot berpetak.  Hasil Penelitian didapatkan 38 spesies dan 122 individu tumbuhan pada hutan primer, 22 spesies dan 49 individu pada hutan bekas tebangan serta 45 spesies dan 120 individu pada hutan tanaman campuran. Kandungan karbon bagian atas tumbuhan hidup pada hutan primer 1.359.884,68 kg/ha, hutan bekas tebangan 610.429,67 kg/ha  dan hutan tanaman campuran 360.793,70 kg/ha.  Kandungan karbon pada serasah hutan primer 774,49 kg/ha, hutan bekas tebangan 521,36 kg/ha dan hutan tanaman campuran 766,20 kg/ha.
Eliminasi Escherichia coli O157:H7 Yang Diisolasi Dari Daging Sapi Di Rumah Potong Hewan (RPH) Dan Pasar Tradisional Ni Wayan Purni wirathi; Retno Kawuri; Ida Bagus Darmayasa
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p08

Abstract

This research was conducted to determine the quality of beef in slaughterhouses and Traditional markets in Denpasar, Badung, and Klungkung, in terms of Escherichia coli and isolation E. coli O157:H7 which are suspected of contaminating beef. Experimental research was conducted in a laboratory with a heating test of E. coli O157: H7 to determine the heat resistance of E. coli O157: H7. Beef taken at Nyanggelan, Panjer market shows the highest value of E. coli which is 222.3 colonies/g, and the lowest value of E. coli is obtained in beef taken at Kaliunda abattoir which is 2.3 colonies/g. The identification results on 39 beef samples showed that all beef samples contained E. coli, 24 samples positive of E. coli O157, and 9 other samples showed positive E. coli O157:H7. The heating treatment at 60? still grows E. coli O157:H7 colonies in the Petri dish, but there has been a decrease in the number of initial colonies before the heating process. All locations were 100% contaminated with E. coli, 61.5% positive E. coli O157, and 25.6% positive E. coli O157:H7. Heating 65? for 15 seconds and 70? for 5 seconds can kill E. coli O157: H7 in beef. Keywords: E. coli O157: H7, beef, slaughterhouses, traditional markets, heating process.
Uji Kandungan Escherichia coli dan Total Bakteri pada Daging dan Organ Dalam Sapi di Rumah Potong Hewan (RPH) Kota Denpasar dan Kabupaten Badung Putu Anjarina; Retno Kawuri; Dwi Ariani Yulihastuti
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p06

Abstract

Penelitian ini ditujukan untuk menguji kandungan E. coli dan cemaran total bakteri daging sapi bagian punggung, daging bagian paha, usus, hati, limfa dan paru di Rumah Potong Hewan (RPH) di Kota Denpasar dan kabupaten Badung, Bali. Hasil kandungan E. coli yang diperoleh dari Rumah Potong Hewan (RPH) Pesanggaran pada sampel paha sebesar 70 APM/100g dan RPH Mambal pada sampel usus sebesar 70 APM/100g. Sampel limfa dari kedua RPH memiliki nilai yang paling rendah yaitu 30 APM/100g. Nilai yang telah dijabarkan dapat dikatakan melebihi ambang batas yang diperbolehkan oleh BPOM (2009) yaitu batas maksimum cemaran E. coli 1 x 101 APM/100g. Hasil penelitian total bakteri yang diperoleh dari sampel yang diambil di RPH Pesanggaran pada sampel limfa menunujukkan hasil yang paling besar yaitu 14x104 CFU/g, sedangkan nilai yang terkecil berasal dari sampel usus yaitu 2x104 CFU/g. Hasil total bakteri yang berasal dari RPH Mambal nilai sampel yang terbesar dimiliki oleh sampel limfa sebesar 13x104 CFU/g, sedangkan nilai hasil terkecil dimiliki oleh sampel paha 1x104 CFU/g diperbolehkan BPOM (2009) yaitu 1x104 CFU/g. Kesimpulan dari penelitian ini menunjukkan sampel daging paha, daging punggung, usus, hati, paru dan limfa memiliki nilai total cemaran E. coli dan cemaran bakteri yang melebihi batas standar yang diperbolehkan oleh BPOM Bali.

Page 11 of 32 | Total Record : 316

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