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Kadek Karang Agustina
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Buletin Veteriner Udayana
Published by Universitas Udayana
ISSN : 20852495     EISSN : 24772712     DOI : https://doi.org/10.24843/bulvet.
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology Public Health Veterinary
The Buletin Veteriner is focused on Veterinary Medicine and Animal Sciences study with its various developments
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 587 Documents
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Penggunaan Gonadorelin dalam Penanganan Keterlambatan Pubertas pada Sapi Bali Anak Agung Adhitya Chandra; I Gusti Ngurah Bagus Trilaksana; Tjok Gde Oka Pemayun
Buletin Veteriner Udayana Vol. 14 No. 5 October 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i05.p17

Abstract

Keterlambatan pubertas merupakan salah satu masalah yang dihadapi oleh peternak sehingga menyebabkan keterlambatan dalam produksinya. Beberapa hormon telah digunakan dalam penanganan kasus keterlambatan pubertas. Penelitian ini bertujuan untuk mengetahui peranan gonadorelin dalam penanganan keterlambatan pubertas pada sapi bali. Sapi bali yang digunakan adalah sapi bali betina yang telah berumur 24 bulan atau lebih yang belum menunjukkan tanda estrus untuk pertama kali (pubertas). Sapi bali betina dibagi menjadi 2 kelompok masing masing terdiri dari 16 ekor. Kedua kelompok diberi perlakuan berupa injeksi gonadorelin dengan dosis untuk kelompok 1 (P1) 50 ?g/ekor dan kelompok 2 (P2) 100 ?g/ekor. Pengamatan untuk diameter folikel dilakukan dengan USG sebelum injeksi gonadorelin dan sesudah munculnya estrus. Pengamatan terhadap munculnya estrus dan intensitas estrus dilakukan 2 kali sehari yaitu pukul 06.00 - 08.00 WITA dan pukul 17.00 - 19.00 WITA. Hasil penelitian menunjukkan bahwa rata rata diameter folikel sebelum injeksi gonadorelin untuk P1 = 4,38 mm dan P2 = 4,41 mm sedangkan saat munculnya estrus rata rata diameter folikel untuk P1 = 7,68 mm dan P2 = 10,83 mm. Rata rata waktu munculnya estrus pada P1 = 6,38 hari sedangkan P2 = 4 hari, sedangkan intensitas estrus pada P1 = 1,5 dan P2 = 2,56. Secara statistik perbedaan diameter sebelum perlakuan tidak bermakna (p>0,05) sedangkan saat estrus terjadi perbedaan yang bermakna (p<0,05) diantara kedua perlakuan. Waktu munculnya estrus dan intensitas estrus secara statistik tidak terdapat perbedaan yang bermakna (p>0,05) diantara kedua perlakuan. Dari penelitian ini dapat disimpulkan bahwa pemberian gonadorelin dapat merangsang perkembangan folikel dan menyebabkan munculnya estrus pada sapi bali betina yang mengalami keterlambatan pubertas.
Deteksi Infeksi Anaplasma sp., Borrelia burgdorferi dan Ehrlichia sp. pada Anjing yang Terinfestasi Caplak di Kota Denpasar Ni Made Devityasih Perayadhista; Nyoman Adi Suratma; Nyoman Sadra Dharmawan
Buletin Veteriner Udayana Vol. 14 No. 5 October 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i05.p16

Abstract

This research has been conducted as its purpose is to detect the infection of Anaplasma sp., Borrelia burgdorferi, and Ehrlichia sp. in dogs as well as it was manifested with ticks in Denpasar City. The detection of these parasites was conducted by serological examination, haematology test, and microscopic identification with blood smear. Blood samples were examined from 30 dogs which is belong to local society and it also invested with Rhipichepalus sanguineus. Serological examination was performed with SNAP® 4DX® Plus test kit. Haematology examination were using haematology analyzer I-CUBIO iCell-800 Vet®. The risk factor was learned by analyzing it with correlation to parasite infection case in dogs with a few factors which is considered being influenced. The result of serological examination shows that 22 of 30 dogs (73.3%) were positively reacted to Anaplasma sp. and Ehrlichia sp. but shown negative to Borrelia burgdorferi. In addition, haematology profile shown anemia, leucocytosis, leucopenia, lymphocitosis, lymphopenia, and thrombocytopenia, as well as abnormalities in blood cell morphology such as: poikilocytosis, and the presence of crenation. There is no correlation between blood pathogenic agents in this research to: age, breed, sex, origin, presence of other pets, or grooming management.
Total Bakteri dan Coliform Limbah Tempat Pemotongan Ayam Tradisional Setelah Perlakuan Serbuk Biji Kelor I Made Merdana; I Ketut Suada; I Made Robi; I Dewa Made Nurja Sadhi
Buletin Veteriner Udayana Vol. 14 No. 5 October 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i05.p04

Abstract

The waste produced by the traditional chicken slaughterhouse industry consists of two types, namely solid, and liquid waste. The main solid waste consists of feathers, feces and discarded body tissue. The Liquid waste consists of washing water from chicken carcasses mixed with blood, traces of fat, and organic materials. Waste that is directly discharged into waterways without treatment has the potential to contain pathogenic microorganisms. Moringa seeds as natural coagulants contain active compounds amnosyloxy benzyl isothiocynate, tannin polyphenols, and cationic polyelectrolyte proteins. The active ingredient has antiseptic, antibacterial, and coagulation-flocculation activities. This study aims to determine the effect of Moringa seed powder concentration and sedimentation time on total bacteria and Coliforms in traditional chicken slaughterhouse wastewater. Bacterial isolation for the calculation of total bacteria using the pour plate method on the nutrient agar culture, and the total Coliform by the spread plate method on the eosin methylene blue agar culture. The results showed that the coagulation flocculation of Moringa seed powder had a significant effect (P<0.05) on the decrease in total log bacteria and Coliform wastewater of traditional chicken slaughterhouses. It can be concluded that the coagulation-flocculation of Moringa seed powder can decrease the total bacterial and Coliform with the best results at a concentration of 150 mg / L and 60 minutes deposition time.
Identifikasi Jenis Lalat Tidak Penghisap Darah pada Peternakan Babi di Bali Alice Viria Cordeiro da Costa Xavier; I Made Dwinata; Ida Bagus Made Oka
Buletin Veteriner Udayana Vol. 14 No. 5 October 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i05.p13

Abstract

Flies are ectoparasites that act as vectors of disease and their existence greatly affects the health of pigs. The purpose of this study was to identify the types and fluctuations in the number of flies that do not suck blood in pig farms in Badung Regency and Denpasar City. The fly collection was carried out on a semi-intensive pig farm with a period of every week for 3 months. In this study, flies were caught using fly paper glue and sweeping net and then euthanized with 70% alcohol. The data obtained were presented descriptively and analyzed using the Wilcoxon test. The identification results showed that the type of non-blood-sucking flies found in pig farms in Badung Regency and Denpasar City was Musca sp. as many as 2,383 tails (96.99%), Lucilia sp. as 56 tails (2.28%) and Fannia sp. as 18 tails (0.73%). During the 12 weeks of observation, fluctuations occurred with the highest decrease in fly population in the 3rd week (November) and 10th (January) while the increase occurred in the 5th week (December) to the 9th week (January). The number of fly population found in the Denpasar pig farm is 1,464 larger, which is largerthan the population in the pig farm in Badung Regency 993 tails.
Profil Hematologi dan Biokimia Darah Babi Bali Asal Peternakan Tradisional dan Konvensional yang Diberi Pakan Komersial Putu Vindhy Chempaka Putri; I Gede Mahardika; Nyoman Sadra Dharmawan
Buletin Veteriner Udayana Vol. 14 No. 5 October 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i05.p14

Abstract

Experimental studies have been carried out to determine the weight gain, blood hematological and biochemical profile of weaning bali pig from traditional and conventional farms which are then given the same treatment as commercial feed. Weighing pigs is carried out every two weeks for two months. Examination of blood samples was carried out three times, at the beginning, middle and end of the study. The hematology profile was examined with a hematology analyzer. Cholesterol, triglycerides, and protein total were examined by photometer. The results showed that the weight gain of weaning pigs from traditional and conventional farms for 8 weeks was 13.81 kg and 14.19 kg (P>0.05). Overall range of erythrocytes; hematocrit; and hemoglobin in this study were 7.47-8.59 x 106/µL; 37.23-44.78 %; and 11.95-14.17 g/dL, respectively. The total leukocyte range is 19.12-28.18 x 103/µL; neutrophils: 0.49-7.19 %; eosinophils: 0.24-2.0 %; basophils: 0.73-5.18 %; lymphocytes: 16.22-83.02 %; and monocytes 1.21-6.82 %. The cholesterol range is 65.85-90.00 mg/dL; triglycerides: 30,75-78,81 mg/dL; and protein total: 5.0-6.6 g/dL. There was no statistically significant difference (P>0.05) between the average erythrocytes, hematocrit, hemoglobin, leukocytes total, leukocytes differential, cholesterol and protein total of piglets originating from traditional farm compared to those from conventional farm. However, there were significant differences (P<0.05) in triglyceride levels.
Perubahan Histopatologi Limpa Ayam Kampung yang Diberikan Jamu Daun Ashitaba dan Divaksin Avian Influenza Velia Chyntia Victoria; I Wayan Sudira; Ida Bagus Oka Winaya
Buletin Veteriner Udayana Vol. 14 No. 5 October 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i05.p07

Abstract

This study aims to determine the effect of giving ashitaba leaf herbal medicine as seen from the histopathological picture of native chicken spleen vaccinated against avian influenza disease vaccine. This research uses a complete random design. The treatment consisted of five treatment groups namely without ashitaba (control), with an ashitaba dose of 50; 100; 200, and 400 mg / head were dissolved with water until the volume is 100 ml and given treatment for 14 days. On the 21st day, all groups of native chickens were vaccinated against the avian influenza vaccine. On the 42nd day, native chickens were euthanasia by cervical dislocation, then the spleen was taken to see changes in the histopathological structure. Then it continued with Hematoxylin-Eosin (HE) staining and was tested under a microscope at 400x magnification. Variables that are seen from the proliferation of lymphoid follicles on white pulp by using a scaling scale for histopathological changes. The research data were analyzed by analysis non parametric Kruskal-Wallis. The results showed that there was a significant change in spleen histopathology between treatment P0 (control) and treatment given the herbal ashitaba leaf. Giving the herbal ashitaba leaf at a dose of 400 mg/head/day improves the body defense system of native chickens vaccinated with avian influenza.
Respon Analgesia, Sedasi, dan Relaksasi Anestesi Ketamin dan Propofol dengan Premedikasi Xilasin pada Sapi Bali Nur Liliana Putri Prihatiningsih; I Gusti Ngurah Sudisma; I Gusti Agung Gde Putra Pemayun
Buletin Veteriner Udayana Vol. 14 No. 5 October 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i05.p10

Abstract

This study aims to determine the response of analgesia, sedation, and relaxation of the use xilasin premedication with anesthesia ketamine, propofol and their combination in bali cattle (Bos sondaicus). Bali cattle are used as many as 12 head which were divided into three treatment groups. This study used a completely randomized design with three treatments which are xilasin combination (0.1 mg) ketamine (2 mg), xilasin combination (0.1 mg) propofol (3 mg), xilasin combination (0.1 mg) ketamine (1 mg) and propofol (1.5 mg). The result of the research showed that ketamine and porofol anesthesia with xilasin premedication in bali cattle had a significant effect (P<0.05) on analgesia, sedation and relaxation responses. The average time for analgesia response is 2.5 – 12.5 minutes with analgesia duration of 5-17.5 minutes. For sedation response the average time of occurrence is 2.5-13.7 minutes with sedation duration of 5-13.75 minutes. The response time fot the occurrence of 5-12.5 minutes of relaxation with relaxation duration 2.5-23.75 minutes. In this study it can be concluded that the xilasin-ketamine-propofol combination is better fo bali cattle anesthesia becauses it produces a faster induction with a longer duration.
Cover Vol. 14 No. 5 October 2022 Kadek Karang Agustina
Buletin Veteriner Udayana Vol. 14 No. 5 October 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tingkat Maturasi Oosit Sapi Bali pada Media TCM 199 dengan Penambahan Hipotaurin Ester Novitasari; Tjok Gde Oka Pemayun; I Ketut Suatha; I Gusti Ngurah Bagus Trilaksana
Buletin Veteriner Udayana Vol. 14 No. 5 October 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i05.p12

Abstract

Maturasi oosit merupakan salah satu langkah penting dalam pelaksanaan fertilisasi in vitro untuk pelaksanaan transfer embryo. Oosit hasil koleksi baik secara aspirasi maupun slicing pada umumnya belum mencapai fase MII yang dibutuhkan untuk fertilisasi in vitro sehingga perlu dilakukan maturasi oosit. Tujuan penelitian adalah untuk mengetahui tingkat maturasi oosit sapi bali pada media TCM 199 dengan penambahan hipotaurin. Oosit berasal dari ovarium yang diperoleh dari rumah potong hewan Pesanggaran Denpasar dan dikoleksi dengan metode slicing. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari tiga kelompok yaitu kelompok I / kontrol (P0: media maturasi tanpa hipotaurin), kelompok II (P1: media maturasi dengan penambahan hipotaurin dengan konsentrasi 2 mMol dan kelompok III (P2: media maturasi dengan penambahan hipotaurin dengan konsentrasi 4 mMol dengan masing-masing kelompok terdiri dari 27 ulangan. Maturasi oosit dilakukan dengan cara inkubasi selama 24 jam dalam inkubator dengan suhu 38,5 0C dengan tekanan CO2 5 %. Hasil penelitian menunjukkan bahwa tingkat ekspansi sel kumulus hingga tahap cumulus oocyte complexes (COCs) pada kelompok P1 tidak berbeda nyata dibandingkan kelompok P0 (p>0,05), namun kelompok P2 menunjukkan perbedaan yang nyata (p<0,05). Pada tingkat kematangan inti, penambahan hipotaurin tidak menyebabkan perbedaan yang nyata (p>0,05) pada tingkat GVBD, namun pada tingkat MI terdapat perbedaan yang nyata (p<0,05) antara kelompok P0 dengan P1 dan P2, sedangkan pada tingkat MII tidak terdapat perbedaan yang nyata (p>0,05) antara P0 dengan P1 namun berbeda nyata (p<0,05) antara P0 dengan P2. Dari hasil peneltian dapat disimpulkan bahwa penambahan hipotaurin dengan konsentrasi 4 mM pada media maturasi oosit TCM 199 dapat meningkatkan tingkat ekspansi sel kumulus mencapai COCs dan meningkatkan tingkat kematangan inti mencapai fase MII.
Kadar Total Protein Serum Sapi Bali Pasca Transportasi di Rumah Pemotongan Hewan Pesanggaran Denpasar Gusti Agung Rama Wiratama Putra; Iwan Harjono Utama; I MAde Merdana
Buletin Veteriner Udayana Vol. 14 No. 6 December 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i06.p16

Abstract

This study aims to determine the effect of transportation on changes in total protein levels in Bali cattle after a trip to the Pesanggaran Slaughterhouse (RPH) Denpasar. Transportation can lead to stress and dehydration. To diagnose the occurrence of stress can be done by biochemical tests. The biochemical test carried out in this study was to measure the levels of total protein in the blood. The object used is Bali cattle that have not been rested after transportation as many as 20 heads at the Denpasar Pesanggaran Slaughterhouse. Blood was taken through the auricular vein and put into a vacutainer tube containing a gel separator to separate serum from blood. Blood serum samples were taken to the Bali Provincial Health Laboratory for immediate examination of total protein levels using the Photometer 5010V5+ machine. The total protein content of the examined samples had the lowest level of 5.8 g/dL and the highest 8.5 g/dL with an average of 7.5 g/dL. From the results of the study, the total protein content of Bali cattle after transportation was considered normal, this indicated that there was no effect of transportation on the total protein content.

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