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Buletin Veteriner Udayana
Published by Universitas Udayana
ISSN : 20852495     EISSN : 24772712     DOI : https://doi.org/10.24843/bulvet.
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology Public Health Veterinary
The Buletin Veteriner is focused on Veterinary Medicine and Animal Sciences study with its various developments
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 587 Documents
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Gambaran Hitopatologi Usus Halus Ayam Kampung yang diberikan Jamu Daun Ashitaba dan Vaksin Avian Influenza I Putu Sandika Arta Guna; I Wayan Sudira; Ni Luh Eka Setiasih
Buletin Veteriner Udayana Vol. 14 No. 6 December 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i06.p04

Abstract

This study aims to determine the effect of giving Ashitaba leaf herbal medicine (Angelica keiskei) which was given orally against the histopathological features of the small intestine of native chickens vaccinated with Avian influenza. The sample used in this study were 25 heads day old chicken native chickens, which were divided into 5 treatment groups. The first treatment was (P0) without being given ashitaba leaf herbal medicine but given aquadest, group (P1) was given ashitaba leaf herbal with a concentration of 50mg / head / day, (P2) at a dose of 100mg / head / day, group (P3) with a dose of 200mg / head / day, and (P4) at a dose of 400mg / head / day. The treatment is carried out every day for 14 days from day 8 to day 21 which is given orally by mixing drinking water and ashitaba leaf herbal powder until the volume is 100. ml. On the 21st day, all groups of chickens were vaccinated with the vaccine Avian influenza. On the 42nd day, the experimental native chicken was necropsed, then the small intestine was taken to see changes in the histopathological structure with Hematoxylin-Eosin ( HE) staiuning. The variables examined included hydrophic degeneration, inflammatory cell infiltration and necrosis. The data obtained was tested Kruskal-Wallis. Statistical test results obtained P-value hydrophic degeneration (P = 1,000), inflammatory cell infiltration (P = 0,833) and necrotic lesions (P = 0,983). The results of this study indicate that giving the ashitaba leaf herbal medicine (A. keiskei) with a dose range of 50 mg / head / day to a dose of 400 mg / head / day for 14 days had no significant effect on the histopathological picture of the small intestine of native chickens seen from hydrophic degeneration, inflammatory cell infiltration and necrosis.
Cover Vol. 14 No. 6 December 2022 Kadek Karang Agustina
Buletin Veteriner Udayana Vol. 14 No. 6 December 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Gambaran Patologi Anatomi dan Histopatologi Organ Pertahanan Itik Bali Pascainfeksi Buatan Avian orthoavulavirus 1 Isolat Tabanan-1/ARP/2017 M. Farhan Al Ma'arif; Anak Agung Ayu Mirah Adi; Ida Bagus Oka Winaya
Buletin Veteriner Udayana Vol. 14 No. 6 December 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i06.p08

Abstract

This research aims to determine the gross pathological and histopathological changes of the thymus, bursa of Fabricius, and spleen of bali ducks infected with newcastle disease virus or Avian orthoavulavirus 1 Tabanan-1/ARP/2017 isolate. This research used 15 male bali ducks aged one week. After the acclimatization period for two weeks and the measurement of ND antibody titer in duck serum showed results below 23 HI units, 10 ducks were infected with AOAV-1 Tabanan-1/ARP/2017 isolate at a dose of 0.5 ml/27 HA units intraocularly (P1) and 5 other ducks as the control (P0) were given 0.5 ml of phosphate buffered saline by the same route. Then necropsy was performed on the dead ducks and gross pathology examination was performed on its immune organs. That organs were processed and stained for Hematoxylin-Eosin, then performed histopathology examination. The results showed that infection with AOAV-1 Tabanan-1/ARP/2017 isolate in bali ducks could cause gross pathological changes in the form of necrotic foci lesions in the spleen and atrophy in the thymus. Meanwhile, the histopathological appearance showed severe depletion of lymphoid cells in the thymus and bursa of Fabricius, and mild lymphoid cell depletion in the spleen.
Korelasi Viskositas Mukous Serviks dengan Konsentrasi Estrogen Saat Estrus pada Sapi Aceh Cut Nila Thasmi; Cantika Tamara Listin; Husnurrizal Husnurrizal; Amalia Sutriana; Herrialfian Herrialfian; Hafizuddin Hafizuddin; Mulyadi Adam; Dasrul Dasrul; Tongku Nizwan Siregar
Buletin Veteriner Udayana Vol. 15 No. 1 February 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v01.i01.p20

Abstract

The viscosity of cervical mucus is affected by the concentration of estrogen. This study aims to determine the relationship between the viscosity of cervical mucus with the concentration of estrogen in Aceh cattle in the success of mating. This study used four female Aceh cattle, aged 3-7 years and weighing 150-250 kg, had given birth, and had two regular estrus cycles. All aceh cattle were synchronized with PGF2? (dinoprost tromethamine inj., Lutalyse®, Zoetis US) by the two shoot method intramuscularly. Cervical mucus collection was performed by aspiration on the day of estrus. The cervical mucus viscosity measurement is done by looking at the spinnbarkeit value. Estrogen concentration was measured by enzyme linked immunosorbant assay (ELISA). The data obtained in this study were analyzed using the Pearson correlation test. The results showed that the average viscosity of cervical mucus in Aceh was 11.8±4.6 cm, and the average concentration of estrogen in Aceh cattle during estrus was 106.5±15.8 pg/ml. The correlation between cervical mucus viscosity and estrogen concentration was 0.983. The conclusion of this study is that the higher the concentration of estrogen, the viscosity of cervical mucus will decrease so that the spinnbarkeit value will increase. It is necessary to conduct further studies related to the electrochemical characteristics of cervical and vaginal mucus during estrus including the value of ferning typology, electrical resistance vaginal mucus (ERVM), pH, sodium, magnesium, calcium and other cations that affect estrus performance in Aceh cattle.
Keragaman dan Korelasi Dimensi Panjang Bagian Kaki Depan dan Belakang Tubuh Induk Sapi Bali di Pusat Pembibitan Sapi Bali Unggul Gerokgak, Buleleng, Bali Adinda Adinda; I Ketut Suatha; Ni Nyoman Werdi Susari
Buletin Veteriner Udayana Vol. 15 No. 1 February 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v01.i01.p12

Abstract

This research was conducted on 25 cows at the Bali Superior Cattle Breeding Center in Gerokgak, Buleleng district. Data was collected by purposive sampling method and then processed using SPSS IBM 26 with descriptive and biplot analysis, then presented in the form of tables and biplot graphs. The results obtained indicate that the most varied coefficient of variance is the length of the upper foreleg of 22.8369%, then followed by the length of the upper hind leg of 19.3704%, the length of the middle hind leg of 17.1938%, the length of the forefoot the lower part obtained a score of 14.3102%, the length of the middle forefoot was 12.0132% and the most uniform was the length of the lower back leg of 10.6815%. The length of the lower hind legs is positively correlated with the length of the lower forelegs, the length of the middle hind legs is positively correlated with the length of the middle forelegs, as well as the length of the upper hind legs is positively correlated with the length of the front legs of the tatas, but the length of the middle forelegs negatively correlated with the length of the upper hind leg and between the length of the middle forefoot and the length of the upper hind leg was not correlated with the length of the upper forefoot. To get a uniform Bali cow, it is necessary to select the length of the upper forelegs.
Histopatologi Hati Tikus yang Terpapar Logam Berat Timbal Elisabeth Karina; I Ketut Berata; Ni Luh Eka Setiasih
Buletin Veteriner Udayana Vol. 15 No. 1 February 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v01.i01.p01

Abstract

Timbal merupakan salah satu bahan pencemar udara yang sangat berbahaya. Toksisitas logam berat Pb dapat menimbulkan dampak yang berat, salah satunya pada organ hati. Penelitian ini bertujuan untuk mengetahui histopatologi hati pada tikus yang terpapar logam berat timbal dengan dosis yang berbeda. Penelitian ini menggunakan tikus putih jantan dengan strain Wistar, umur 2 bulan dan berat badan 250-300 g. Tikus yang digunakan 20 ekor dibagi atas empat kelompok perlakuan yaitu kontrol (P0), Pb asetat 0,5 ppm (P1), Pb asetat 1,0 ppm (P2), dan Pb asetat 2,0 ppm (P3) selama 30 hari. Pada hari ke 31 dilakukan nekropsi lalu organ hati diambil dan dimasukkan ke dalam netral buffer formalin 10%. Setelah organ hati difiksasi, dilakukan pembuatan preparat histopatologi menggunakan pewarnaan Hematoksilin dan Eosin. Pemeriksaan histopatologi dilakukan meliputi tiga variabel lesi yaitu kongesti, degenerasi melemak, dan nekrosis. Tingkat keparahan lesi tersebut dibuat skoring yaitu 0, 1, 2 dan 3 masing-masing apabila normal, lesi ringan, sedang dan berat. Data kemudian dianalisis dengan menggunakan uji non parametik Kruskal-Wallis dan Mann-Whitney. Hasil penelitian menunjukkan bahwa pemberian logam timbal dengan kandungan 0,5 ppm, 1,0 ppm, dan 2,0 ppm menyebabkan lesi histopatologi hati tikus berupa kongesti dan nekrosis yang signifikan dibandingkan kontrol, kecuali lesi degenerasi melemak.
Deteksi Titer Antibodi Newcastle Disease pada Broiler yang Divaksinasi di Pembibitan Umur Satu Hari Ketut Ari Andhita Badraresta Arnaya; Ida Bagus Bagus Kade Suardana; Tjokorda Sari Nindhia
Buletin Veteriner Udayana Vol. 15 No. 1 February 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v01.i01.p17

Abstract

Vaccination when the maternal antibody is high may cause neutralization of the antibody thus the immunity response is not most optimal. The measurement of titer antibodies after vaccination is an indicator to define success of the vaccination and it can be tested using the Hemagglutination Inhibition serology. This research aims to acknowledge until what age the titer antibodies shown seropositive on broilers which are vaccinated on the one-day-old hatchery. The data was obtained by random sampling from the broilers population which are taken 4 times on the day 7, day 14, day 21 and day 28. The data then is being analyzed using analysis method of variance, which is continued with regression analysis. The research result shows that broilers which have been vaccinated on the one-day-old hatchery only produce seropositive titer until 13 days old with 4.2 HI log 2 titer and began to decrease at 14 days of age with 3.9 HI log 2 titer. The conclusion of this study is that broilers vaccinated on one-day-old hatchery produced seropositive titers up to 13 days of age. Suggestions need to be given a booster vaccination at the age of 13 days in broilers vaccinated on the one-day-old hatchery.
Isolasi dan Identifikasi Bakteri Shigella spp. Penyebab Diare pada Anjing Voni Cornelia Br Sembiring; I Gusti Ketut Suarjana; Ketut Tono Pasek Gelgel
Buletin Veteriner Udayana Vol. 15 No. 1 February 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v01.i01.p08

Abstract

Dogs are one of the favorite animals that are kept by many people because they are considered intelligent and loyal animals. One of the main health problems of dogs is diarrhea caused by a bacterial infection. Shigella spp. is a pathogenic bacterium in the digestive tract of dogs. This study aims to isolate and identify Shigella spp. bacteria that cause diarrhea in dogs. Samples studied using rectal swabs in dogs showing clinical symptoms of diarrhea with a total of 48 samples from veterinary clinics in the city of Denpasar and its surroundings. Isolation of Shigella spp. was conducted on Eosin Methylene Blue Agar, Salmonella-Shigella Agar, and Sheep Blood Agar. Colonies that grew and separated on Salmonella-Shigella Agar medium were subjected to Gram staining, catalase test, oxidase test and continued with biochemical tests, namely Triple Sugar Iron Agar, Sulfid Indole Motility, Methyl Red Voges Proskauer, Simmons Citrate Agar, and test for sugars such as glucose, lactose, mannitol, and arabinose. The results showed that of the 48 isolated and identified samples, one sample showed a positive result for Shigella spp. with possibly the species Shigella dysentriae. It was concluded that one (2.08%) of Shigella spp. bacteria could be isolated and identified from 48 samples of dog rectal swabs that showed clinical symptoms of diarrhea. It is necessary to conduct further research on the level of pathogenicity of Shigella spp. bacteria and for further research, it is necessary to supplement the API 20E test to determine the Shigella species.
Gambaran Struktur dan Morfometri Usus Halus Itik Bali pada Umur Berbeda Umi Reston; I Ketut Suatha; Luh Gde Sri Surya Heryani; Ni Luh Eka Setiasih
Buletin Veteriner Udayana Vol. 15 No. 1 February 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v01.i01.p13

Abstract

The Bali duck is one of the original Indonesian germplasm whose distribution is endemic in Bali. This study aims to determine the anatomical structure, histology, and morphometry of the small intestine of Bali ducks at different ages. In this study, samples of the small intestine of Bali ducks aged 1 month, 3 months, and 5 months were used as many as 18 male Bali ducks. Bali ducks were obtained from one of the duck farms in Mengwi District, Badung Regency, Bali Province. Sampling of the small intestine was carried out in three parts, namely the duodenum, jejunum and ileum, then the organs were fixed with 10% NBF which was then made histological preparations with HE staining. The research results are presented in the form of qualitative and quantitative descriptive. The results showed that the anatomical structure of the small intestine, namely the duodenum, jejunum and ileum, had the same composition, boundaries and shape in ducks of different ages, while the histological structure of the small intestine, namely the duodenum, jejunum and ileum, was composed of four layers, namely the tunica mucosa, tunica submucosa. tunica muscularis and the outermost is the tunica serosa. Small intestine morphometry results in Balinese ducks at different ages had a significant difference at 1 and 3 months, 1 and 5 months, whereas in ducks at 3 and 5 months they were not so significant in the duodenum, jejunum and ileum. Based on the research conducted, it can be concluded that there is no change in the anatomical structure in terms of composition, boundaries, and shape, while histologically , each Balinese duck small intestine is composed of four layers including the tunica mucosa, tunica submucosa, tunica serosa and serosa, while based on the results of intestinal morphometry fine bali ducks have significant differences in each age of bali ducks both in the duodenum, jejunum and ileum, so it is necessary to do further research on female ducks and perform histomorphometry on each layer.
Prevalensi Infeksi Cacing Nematoda Gastrointestinal pada Ayam Petelur di Desa Peninjoan, Kecamatan Tembuku, Kabupaten Bangli, Bali Agostinho Moreira Belo; I Nyoman Adi Suratma; Ida Bagus Made Oka
Buletin Veteriner Udayana Vol. 15 No. 1 February 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v01.i01.p03

Abstract

Nematodes are among the most important class of worm parasites in poultry in terms of the number of species and the damage they cause. Common nematode worms that infect the digestive tract of chickens include: Capilaria spp, Tetrameres spp, Aquaria spp, Ascaridia galli, Strongyloides avium, and Heterakis gallinarum. Risk factors that influence the incidence of parasite infection are influenced by; host, parasites and environment. Host factors that most commonly affect the incidence of infection include: type, age, sex, while parasite factors that affect infection include: mode of transmission, viability (survival), pathogenicity and immunogenicity, as well as environmental factors that influence, especially: weather, enclosure sanitation and humidity. The purpose of this study was to determine the prevalence of nematode grastrointestinal infection in laying hens in Tembuku Sub-district, Bangli District, Bali. The object of this study is feces from laying hens taken directly as many as 240 laying hens. The inspection method used is qualitatively using the floating method. The result showed that the prevalence of gastrointestinal nematode worm infection in laying hens was 9,17 %. The result of the data analysis showed that there was no relationship between the age of the laying hens and the prevalence of gastrointestinal nematode worn infection in laying hens reared in Peninjoan village, Tembuku Sub-district, Bangli district, Bali.

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