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I G. Made Krisna Erawan
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krisnaerawan@unud.ac.id
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Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia
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INDONESIA
Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
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Articles 11 Documents
Search results for , issue "Vol 11 No 4 (2010)" : 11 Documents clear
Memegang Hewan Rentan dan Menangani Produknya Berisiko Besar Tertular Antraks Kulit di Daerah Endemis Chaerul Basri; Nuning Maria Kiptiyah
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objectives of this study were to identify risk factors associated with historical of animals contact and animalproducts on the incident of cutaneous anthrax in humans in Bogor District. The research designed used in this study CaseControl of Observational Epidemiology. Patients of cutaneous anthrax disease record in Puskesmas (Center for HealthServices) were used as a case. Inhabitants in Bogor district living in the same area with patients of cutaneous anthrax andnot showing clinical signs of cutaneous anthrax. The data were collected by structured interviews and direct observations.Data analysis was carried out in three steps, consisting univariate for analysis of frequency distribution, bivariate withChi-square and also multivariate analysis for prediction model of logistic regression. All analysis processed by SPSS 13.0.It can be concluded that the first risk factor associated with the occurrence of cutaneus anthrax was holding susceptibleanimals with Odds Ratio (OR) of 6.648 (95% Confidence Interval (CI) = 2,914-15,167), the second risk factor was meathandling with OR of 5.318 (95% CI: 1,801-15,702). It showed that for people who live in endemic area of anthrax, holdingsusceptible animals sixth times more likely get cutaneous anthrax.
Konsentrasi Serum Anjing yang Optimum untuk Menumbuhkan dan Memelihara Babesia canis dalam Biakan Tutuk Astyawati; Retno Wulansari; Cahyono -; Ferry Ardhiansyah; Ari Rumekso; Dhetty -
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The use of cultivation system in vitro is very important in the future study of Babesia canis. Theaim of this study was to cultivate B. canis in vitro using RPMI media with different concentration of dogsera. B. canis infected erythrocytes were collected from splenectomized infected dog. Parasites werecultivated with RPMI 1640 medium supplemented with normal dog sera at the concentration of 10%, 20%and 40%, the culture were then incubated in 5% CO2 , 37oC temperature for 17 days and subcultured every48 hours. The Percentage of Parasitized Erythrocytes (PPE) in culture with 10% dog serum was significantlylower than those 20%, and 40% The used of 20% and 40 % sera were better than 10%. It is recommendedthat 40 % serum can be used for initiation phase of cultivation, whereas 20% concentration were used formaintenance of the culture.
Peranan Pedagang Unggas dalam Penyebaran Virus Avian Influenza I Nyoman Suartha; I Made Suma Antara; I Kadek Saka Wiryana; I Made Sukada; I Wayan Wirata; Ni Made Ritha Krisna Dewi; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A questionnaire surveillence have been carried out in three different traditional markets (ie. Beringkitin badung district, Kumbasari in Denpasar, Kediri in Tabanan district) in order to understand the role ofpaultry traders behavior in transmitting of avian influenza virus. Of 150 quationares collected most oftraders (66.7%) kept the animals for 1-3 days before it was marketed. Traders bin Beringkit and Kediri(76.3%) used to mix different species of birds in their cages, whereas none of the traders from Kumbasaridoing that. When hygienec and sanitation aspects were considered (ie. Washing and desinfectan sprayingfor cages) it was found that the behavior of traders varied markedly between the 3 different market. Inconclusion the traders awareness to especially bird flue infection and implementation of biosecurity isvery low.
Penerapan Metode Diagnosis Cepat Virus Avian Influenza H5N1 dengan Metode Single Step Multiplex RT-PCR Aris Haryanto; Ratna Ermawati; Medania Purwaningrum; Dini Wahyu Yudianingtyas; Michael Haryadi Wibowo; Charles Rangga Tabbu
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Avian influenza (AI) virus is a segmented single stranded (ss) RNA virus with negative polarity andbelong to the Orthomyxoviridae family. Diagnose of AI virus can be performed using conventional methodsbut it has low sensitivity and specificity. The objective of the research was to apply rapid, precise, andaccurate diagnostic method for AI virus and also to determine its type and subtype based on the SingleStep Multiplex Reverse Transcriptase-Polymerase Chain Reaction targeting M, H5, and N1 genes. In thismethod M, H5 and NI genes were simultaneously amplified in one PCR tube. The steps of this researchconsist of collecting viral RNAs from 10 different AI samples originated from Maros Disease InvestigationCenter during 2007. DNA Amplification was conducted by Simplex RT-PCR using M primer set. Then, bysingle step multiplex RT-PCR were conducted simultaneously using M, H5 and N1 primers set. The RTPCRproducts were then separated on 1.5% agarose gel, stained by ethidum bromide and visualized underUV transilluminator. Results showed that 8 of 10 RNA virus samples could be amplified by Simplex RTPCRfor M gene which generating a DNA fragment of 276 bp. Amplification using multiplex RT-PCRmethod showed two of 10 samples were AI positive using multiplex RT-PCR, three DNA fragments weregenerated consisting of 276 bp for M gene, 189 bp for H5 gene, and 131 bp for N1. In this study, rapid andeffective diagnosis method for AI virus can be conducted by using simultaneous Single Step Multiplex RTPCR.By this technique type and subtype of AI virus, can also be determined, especially H5N1.
Seleksi Kemampuan Pematangan Oosit Domba Menggunakan Teknik Brilliant Cressyl Blue Mohamad Agus Setiadi; Iman Supriatna
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

In present study the developmental competence of sheep oocytes to reach maturation at secondmetaphase (M II) was observed following selection of oocytes using brilliant cressyl blue (BCB).Immature oocytes were harvested from ovaries collected at abattoir; the selected according to theircolour appearence (cytoplasm colour) after being exposed to BCB and incubated for 90 minutes at5% CO2 incubator at 39oC. The selected oocytes were grouped into two based on their cytoplsmcolour i.e. group of oocytes (BCB+) with blue cytoplasm and growing oocytes (BCB-) the unstainedcytoplasm. The control group including freshly collected oocytes which were then selected usingroutine method by observing morphological character under microscope. Each treated group ofoocytes (BCB+ and BCB-) and the control were processed for maturation into culture media (TissueCulture Medium199+10 IU/ml Pregnant Mare Serum Gonadothropine+10 IU Human ChorionicGonadothropine+1?g/ml estradiol benzoat +10% fetal bovine serum) then incubated for 24 hours at5% CO2 incubator at 39oC. Finally oocytes from each treated group and the control were stainedwith arceto orcein 2% to observe the number of oocytes which reach maturatuion at M II. Theresult showed that the percentage of oocytes reaching M II were significantly higher in BCB+ group(54%) compared to BCB- group (8%). It is concluded that BCB is a potential method for selectionofcompetent oocytes
Peningkatan Konsentrasi Testosteron pada Tikus Akibat Paparan Ekstrak Air Biji Pinang Muslim Akmal; Chanif Mahdi; Aulanni’am -
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Areca catechu which is known in Indonesia as pinang, contains alkaloids such as arecoline, arecaine,arecaidine, arecolidine, guvacine, guvacoline, and isoguvasine. Arecoline has an ability to change gonadmorfofunction, including shape abnormality of sperm. The aim of this research was to find out the prospectof extract betel nut of A.catechu as male anti fertility agents based on its activity to increase the testosteroneconcentration. Animal models used consisted of 5 groups of 2-3 months male rats (Rattus norvegicus,Wistar strain) and induced for 1 week by water extract of betel nut at the dose of 0, 1, 2, 3, and 4 gram/200gram body weight. Testosterone concentration was determined by ELISA technique. The result showedthat extract betel nut of A. catechu is potential source of natural and beneficial male anti fertility agentsas it can increase the testosterone concentration.
Pewarisan Karakter Fenotip Ayam Hasil Persilangan Ayam Pelung dengan Ayam Cemani Budi Setiadi Daryono; Iwan Roosdianto; Hendry Tri Sakti Saragih
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The germ plasm variability of Indonesian local chicken is well known and one species which known as“ayam Pelung” originated from West Java is famous for its meat. The present study aimed to observed thephenotype characters i.e. fur color and body weight in a crossbreed hybrids of Pelung and Cemani chickenas a potential meat type breed. Firstly, the breeding between Pelung (one male) and Cemani (4 Females)was performed in semi intensive cage. Secondly, experiment were undertaken using 4 groups of DOC (eachcontain 5 males DOC) : 1) DOC of broiler Cobb 500 vs broiler Cobb 500 ; 2) DOC of Pelung vs Pelung ; 3)DOC of Pelung vs Cemani ; and 4) DOC of Cemani vs Pelung. The animals were kept for 7 weeks (49 days)every week the animals body weight and fur color were recorded. The result showed that there weredifferences in the phenotype characters of between the different crossbreeds. The fur of 5 F1 (femaleCemani vs male Cemani) was black with average body weight 532 ± 39.294 g at week 7. Whilst the fur colorof majority (4/5) F1 (female Pelung vs male Cemani) was black with brown dots with average body weight570 ± 14.445 g at week 7. When Pelung was cross within the same species their F1 fur color was blendedbetween black, brown and whitish with average body weight 652 ± 33.846 g at week 7. It is concluded that,the parent stock in this case the Cemani play a major role in fur color character whereas there ware nodifferences in the body weight at the F1 between the two different parent stock (ie. Pelung, Cemani).
Studi Patologi Kejadian Cysticercosis pada Tikus Putih I Ketut Berata; Anak Agung Gde Arjana; I Wayan Sudira; I Made Merdana; I Ketut Budiasa; Ida Bagus Made Oka
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Rats are commonly used as animal model in pathological and reproduction research, butunfortunately they are often infected with cysticercosis. The objective of this research was to determinethe pathological changes the of the rats (Rattus novergicus) tissues affected with cysticercus. Thisresearch using 24 of female rats. They were adapted to a new environment for a week and the feeding andwater were provided ad libitum. At the end of adaptation period rats were necropsied and the visceralorgans were examined for pathological changes especially the present of cysticercosis. The liver and kidneyof each rat were soaked in 10% phosphate buffered formalin. Following dehydration process, tissue wereembedded in paraplast, cut at 5 micron and stained with Harris hematoxylin eosin (HE). The resultshowed that 8 of 24 rats were affected by cysticercosis on the liver. The histopathological changes werenecrotic lesions and eosinophylic cells infiltration around the cysticercosis lesion. The results showed that8 of 23 rats were affected by cysticercosis. The presence of necrosis and cells inflammation could interferethe results of the study when such a rats are used. It is therefore necessary to screen rats for cysticercosis.
Identifikasi Escherichia coli O157:H7 serta Deteksi Gen Shiga Like Toxin 1 dan 2 Asal Feses Hewan, Daging, dan Feses Manusia I Wayan Suardana; Wayan Tunas Artama; Widya Asmara; Budi Setiadi Daryono
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Escherichia coli O157:H7 with the ability to produce shiga-like toxin was isolated from beef, cattle,chicken, and human feces. Due to its importance to human health, it is necessary to identify the genesencoding the production of shiga-like toxin, stx1 and stx2 respectively to further understand the pathogenesis.Isolation of E. coli was done on Eosin Methylene Blue Agar (EMBA), followed by identification on SorbitolMacConkey Agar (SMAC), latex agglutination test, and H7 antiserum test, respectivelly. The existence ofgenes stx1 and stx2 in E. coli O157:H7 was confirmed molecularly using PCR method with specific primersLP 30/31 and LP 43/44, Stx2 (F)/Stx2 (R) respectively. Escherichia coli O157:H7 was isolated from 22 outof 344 samples (6,4%). Some isolates showed gene stx1 and stx2 was detected in two isolates as indicatedby a 384 bp band (stx1 gene), 584 bp and 1588 bp bands (stx2 gene) respectivelly. The results indicatedthat local isolates E. coli O157:H7 are potential as a zoonoses agent.
Immunological Detection of Rabies Virus in Brain Tissues of Infected Dogs by Monoclonal Antibodies Nyoman Mantik Astawa; Ida Bagus Kade Suardana; Luh Putu Agustini; Faiziah -
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

In order to establish an immunological detection of rabies virus in tissues of infected dogs, monoclonalantibodies (mAbs) against rabies virus (RV) were produced. The mAbs were produced by fusion of mielomacells with the lymphocytes of mice immunized with RV. The mAbs produced were then characterized andused for the detection of rabies virus in brain tissues of infected dogs. Six mAbs designated CC6, EG4,DG10, BB12, CA9 dan EB5 were used in this study. In Western blotting test, some mAbs reacted with 66KDa which is the glycoprotein of the virus. In immunoperoxidase, 2 mAbs (CC6 and DG10) detected RVin the brain of infected dogs. By direct immunoflourescence, flourescence isotyocyanate (FITC) labelledDG10 mAbs detected RV in fresh and formaldehyde fixed brain tissues. RV was detected in 12 infecteddogs but not in normal uninfected dogs. In this study it was confirmed that rabies virus can be detected inthe brain tissues of infected dogs by monoclonal antibodies.

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