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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 18 Documents
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Search results for , issue "Vol 14 No 4 (2013)" : 18 Documents clear
Prevalensi dan Faktor Resiko Kawin Berulang pada Sapi Perah pada Tingkat Peternak (PREVALENCE AND RISK FACTORS OF REPEAT BREEDING IN DAIRY COWS AT THE FARMER LEVEL) Surya Agus Prihatno; Asmarani Kusumawati; Ni Wayan Kurniani Karja; Bambang Sumiarto
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Repeat breeding is a syndrome which affected the reproduction and production efficacy of dairy cattle.The cause of this syndrome may be a herd problem or a variety of individual cow problems. This studyaimed to determine the prevalence and factors which contributed in the repeat breeding syndrome in dairycattle farms in Daerah Istimewa Yogyakarta (DIY). A total of 922 dairy cows which owned by 401 farmerswere used in this study. The cows had a normal estrus cycle, have had at least once calved, age 2.5-8 yearsold, in good health condition, raised traditionally by a farmer or communal system. Multistage and clustersampling method were used in this study. The total numbers of sample at each stage was determined by proportional, whereas the total numbers of farmer was determined using “sampel rambang” Data werecollected by interviewing farmers and direct observation at the farm. Data collected were farmer’s education,length of having farm, farm condition, distance from inseminator, farmer’s ability to detect estrus andestrus cycle, the farm and cow’s hygiene. Data were analyzed by descriptive statistics then followed by ChiSquare and Odds Ratio. The results showed that the prevalence of repeat breeder at the dairy farms was29.4%. Risk factors at the herd level were the most common i.e.: estrus detection once per-day (OR = 17.8);estrus detection twice per-day (OR = 7.9); unsightly sewer (OR = 10.0); soil floor of enclosure (OR = 2.6); andthe use of wells or rivers as source of water (OR = 2.0 and OR = 1.8, respectively.
Enhancement of Nonspecific Immune Response and Growth Performance of Litopenaeus vannamei by Oral Administration of Nucleotides (PENINGKATAN RESPONS IMUN NONSPESIFIK DAN PERFORMA PERTUMBUHAN LITOPENAEUS VANNAMEI MELALUI PEMBERIAN NUKLEOTIDA SECARA ORAL) Henky Manoppo; Sukenda .
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research evaluated the nonspecific immune responseand growth of Litopenaeus vannamei fednucleotide diet.  In Laboratory, juveniles were reared in two groups of glass aquaria, each with threereplications.  Shrimps in group one were fed nucleotide diet and in group two were fedpellet four consecutiveweeks. Total Haemocyte Count and Phenoleoxydase activity were evaluated at the end of feeding whilegrowth was measured at two weeks interval. At the end of feeding, shrimps were intramuscularlyinjectedwith Vibrio harveyi  0.1x106 cfu.shrimp-1.  In tambak, juveniles were raised in two groups of net cages(hapa), each with three replications. One group was fed nucleotide diet while the other wasfed pellet forfour weeks. Total Haemocyte Count of shrimp fed nucleotide diet significantly increased up to 87% higherthan shrimps fed pellet.  Phenoleoxydase activity of shrimp fed nucleotides diet also increased isignificantlyas compared to shrimp fed pellet (p=0.02). Higher resistance and growth were observed in shrimp fednucleotide diet. In tambak, weight gain of shrimp fed nucleotide was 35.75% greater than shrimp fedpellet. Survival rate (83.24%) was higher than shrimp fed pellet (81.71%).  As conclusion, oral administrationof nucleotide at 400 mg.kg-1 diet could enhancethe nonspecific immune response, resistance, and growth ofL. vannamei.
Ekstrak Buah Delima Terstandar Menurunkan Derajat Fibrosis Hati pada Hewan Model Tikus Putih (STANDARDIZED POMEGRANATE FRUIT EXTRACT REDUCES LIVER FIBROSIS DEGREE ON ALBINO RATS (RATTUS NORVEGICUS) AS AN ANIMAL MODEL) Wiwik Misaco Yuniarti; Retno Handajani; Hernomo Ontoseno Kusumobroto; Ketut Sudiana
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study was to analyse the effect of standadized pomegranate fruit extracton liver fibrosis due to biliary obstruction.  Liver fibrosis was induced with bile duct ligation (BDL)technique.  Examinations were performed on the expressions of collagen type 1 and the degree ofliver fibrosis. Thirty two male albino rats (Rattus norvegicus), 2.5 month old, weighing 160-190grams were divided into four experimental groups.  The first group (P0) consisted of rats  whichunderwent laparotomy and treated with 2 ml of  carboxymethyl cellulose (CMC) 0.3%.  Three othergroups consisted of rats which underwent laparotomy and bile duct ligation (BDL) but receiveddifferent treatments.  Group P1 was given carboxymethyl cellulose (CMC) 0.3%, P2 was treatedwith standardized pomegranate fruit extract 150 mg/kgBW/po/day within CMC 0.3% , and P3 wasgiven ellagic acid (EA) 60 mg/kgBW/po/day of equal volume.  Treatments were administered on thesecond day after BDL for 21 days.  The livers were excised one day after the last administration.Observations were made on the expressions of collagen type 1 and the degree of liver fibrosis. Theresults showed that standardized pomegranate extract can inhibit the expression of collagen type Isignificantly in the P2 and P3 compared with P1 group (p <0.05). Treatment with standardizedpomegranate fruit extract also significantly suppressed the progression of liver fibrosis comparedto P1, eventhough there was more liver fibrosis compared to P0 (p<0,05).  The degree of liverfibrosis was not significantly different between P2 and P3, and between P3 and P1 (p>0,05).The administration of standardized pomegranate fruit extract 150 mg/kgBW/po/day  exertedantifibrotic effect by inhibiting the increase the expression of collagen type I and the degree of liverfibrosis.
Fetal Bovine Serum dalam Pengencer Tris Mempertahankan Kehidupan dan Keutuhan Membran Plasma Spermatozoa Semen Beku Domba Garut (FETAL BOVINE SERUM IN TRIS EXTENDER MAINTAINING SPERMATOZOA VIABILITY AND PLASMA MEMBRANE INTEGRITY OF GARUT RAM FROZEN SEMEN Muhammad Rizal; Herdis .; Insun Sangadji
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study was to examine the effectiveness of fetal bovine serum (FBS) against thequality of garut ram frozen semen. Semen was collected from one mature garut ram using artificial vagina.Fresh semen were evaluated then divided into four tubes at equal volume and each tube were diluted withTris extender containing 20% egg yolk (TEY-20, as control); TEY-20 + 8% FBS (FBS-8); TEY-20 + 10% FBS(FBS-10); and TEY-20 + 12% FBS (FBS-12), respectively. Semen at the concentration of 100x106 motilespermatozoa was loaded in 0.25 ml mini straw. Semen was equilibrated at 50C for three hours, then freezeand stored in liquid nitrogen container. The quality of the spermatozoa including percentages of motileand live spermatozoa, intact plasma membrane (IPM) were evaluated following diluting, equilibratingand thawing process. A Complete Randomized design using four treatments and five replicates were usedin the study. The results showed that there was no significant difference (p<0.05) in percentage of motilespermatozoa following thawing between the control (44.0%) and FBS-8 (46.0%), FBS-10 (48.0%), andFBS-12 (47.0%), respectively. The percentage of live spermatozoa and IPM were significantly higher (p<0.05)in the FBS-8 (69.0% and 58.2%); FBS-10 (72.4% and 61.2%); FBS-12 (72.2% and 64.4%) compared to thecontrol (64.8% and 52.8%), respectively. In conclusion, the addition of FBS into Tris extender was able tomaintain the viability and integrity of plasma membrane of garut ram frozen semen.
Keragaman Fenotipik dan Pendugaan Jarak Genetik pada Ayam Lokal dan Ayam Broiler Menggunakan Analisis Morfologi (PHENOTYPIC VARIATION AND ESTIMATION GENETIC DISTANCE BETWEEN LOCAL CHICKEN AND BROILER CHICKEN USING MORPHOLOGICAL ANALYSIS) Harini Nurcahya Mariandayani; Dedy Duryadi Solihin; Sri Sulandari; Cece Sumantri
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This aim of the research was to study the morphological characteristic and estimating genetic distancebetween local chicken and broiler chicken with discriminant and canonical analysis. This research washeld in Faculty of Animal Husbandry, Bogor Agricultural University, using 25 sentul chickens,  25  kampongchickens, 25 kedu chickens , 25  pelung chickens and 25 broiler chickens. The variable as the length ofshank, beak length, back length, chest depth and chest width were measured in this study. The collecteddata were analyzed by using SAS  and SPSS package program. Kampung chickens were mixed with sentulchickens (17.60 %) and kedu chickens (17,70 %). Kedu, kampong,  and  sentul chickens have a relativelyclose genetic distance   compared the genetic distance to pelung chickens with the kampung, sentul, andkedu chickens. Fenogram tree show that there were three separate groups of chickens at the age of eightweeks i.e. : (1) pelung chickens (2), kedu, kampong, and sentul chickens, (3) broiler chickens.  Fenogram treealso shows two separate groups : (1) pelung chickens (2) kedu, kampong, and Sentul chickens (at the age of28 weeks chicken).  The crossbreed between kedu and sentul chickens, also have a relatively close geneticdistance. The phenotypic size of  chickens giving a strong influence on the distinction variable of chickengroups were body length and chest circumference.
Kandidat Vaksin Potensial Streptococcus agalactiae untuk Pencegahan Penyakit Streptococcosis pada Ikan Nila (Oreochromis niloticus) (POTENTIAL VACCINE CANDIDATE OF STREPTOCOCCUS AGALACTIAE FOR PREVENT STREPCOCOCOSIS ON NILA TILAPIA (OREOCHROMIS NILOTICUS) Esti Handayani Hardi; Sukenda .; Enang Harris; Angela Mariana Lusiastuti
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The effectiveness of Streptococcus agalactiae vaccine in tilapia (Oreochromis niloticus) was evaluatedfor prevention of streptococcal disease. The vaccine was prepared using formalin-killed whole cell andconcentrated extracellular products/ECP (62.3 and 55.8 kDa) of â-haemolityc isolate and 62.3; 55,8 and51.8 kDa protein of  non-haemolityc ECP of S. agalactiae.  Vaccination and challenged (103 colony-formingunits (CFU)/fish of â-haemolityc and 105 CFU/fish of non-haemolityc S. agalactiae) trial was conducted byintraperitonial (IP) injection into fish with average body weight of 15 g.  Fish were vaccinated with wholecell, ECP and mixed (whole cell and ECP) vaccine.  Tilapia vaccinated with whole cell of â-haemolitycisolate had a relative percent survival (RPS) rates higher than those of ECP â-haemolityc vaccine. However,fish  vaccinated with mixed (whole cell and ECP) of â-haemolityc has a better protection rates as comparedto those of two type of S. agalactie infection. Whereas those vaccinated with mixed (whole cell non-haemolitycand ECP of â-haemolityc) vaccine has protection rate of 79% from â-haemolityc and 42% from non-haemolitycinfection.  Tilapia vaccinated with whole cell of non-haemolityc was only able to protect fish from non-haemolityc infection and was unable to protect fish from other types.  Tilapia vaccinated with ECP non-haemolityc had a worse RPS than others vaccines in which mix whole cell and ECP vaccine of non-haemolitychad a protection 50-56% from  S. agalactiae infection. Whereas vaccinated with mixed (whole cell â-haemolityc and ECP of non-haemolityc) vaccine showed a better to protect from â-haemolityc than non-haemolityc infection.  It showed thatvaccination with mixed (whole-cell and extracellular product)  vaccineof S. agalactiae â-haemolityc  was more effective to protect tilapia against Streptococcosis.
Aktivitas Ekstrak Daun Kelor terhadap Respons Imun Humoral pada Mencit yang Diinfeksi Salmonella typhi (ACTIVITY OF KELOR LEAF EXTRACT ON HUMORAL IMMUNE RESPONSE IN MICE POST SALMONELLA TYPHI INFECTION) Mohammad Hefni; Muhaimin Rifa’i; Widodo .
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this research was to analyze the activity of  kelor (Moringa oleifera Lam) leaf extract onhumoral immune response in mice infected with Salmonella typhi. Mice were divided into two groups : non-infected and infectedS. typhi groups. Each group was administered orally for 20 days with varied doses ofkelor leaf extract i.e. dose (0 mg/kg BW), dose 1 (14 mg/kg BW), dose 2 (42 mg /kg BW), and dose 3 (84 mg/kg BW).  Then all of the sample in infected groups were injected with 108 cells S. typhi. The humoralimmunity responses were determined by observing the number of lymphoid B cell (B220) and naive Thecell (CD4+CD62L+) by using software BD CellQuest Flowcytometry. The data were analysed using Two-Way ANOVA (P<0.05), with SPSS 16.0 for Windows.  The kelor leaf extract showed imunostimulatoryactivity by significantly improved the number of lymphocyte B cell (B220),  and naive Th Cell (CD4+CD62L+)in mice infected with S. typhi.  The lower doses (dose of 14 mg/kg BW, and 42 mg/kg BW) of kelor leafextracts was more effective than the highest dose (84 mg/kg BW). On the other  hand, the high dose showedimunosupresor activity on naive Teessor Th Cell.  However, immunosupressor activity on naïve Th cell wasobserved on the mice given the highest dose of extract.
Potensi Protein Reseptor Fertilisasi Zona Pelusida Kambing Sebagai Kandidat Imunokontrasepsi dengan Fertilisasi in vitro pada Sapi (POTENTIAL OF FERTILIZATION RECEPTOR PROTEIN GOAT ZONA PELLUCIDA AS CANDIDATE OF IMMUNOCONTRACEPTION BY USING IN VITRO FER Sri Mulyati; Imam Mustofa; Laba Mahaputra
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Studies on the role of zona pellucida glycoprotein-3 (ZP3) in immunocontraception have been conductedin many species including goats (gZP3). Our previous study indicated that gZP3 effective in preventingpregnancy in mice. The aim of this study was to prove the evidence of cross reaction in gZP3 to preventfertilization in cow in vitro. Antibody of gZP3 was produced in mice. Immunized mice serum was analyzedusing ELISA technique and dot blot method. Sperm from frozen semen were processed then incubated incapacitation media supplemented with the gZP3, whilst the cow oocyte was incubated in maturationmedia supplemented with antibody of gZP3. Following this, the normal  in vitro fertilization (withoutincubation neither in gZP3 nor in gZP3 antibody) was performed, respectively. The results showed thatantibody titer of immunized mice serum was higher (p<0.05) than the control group. Dot blotting analysisshowed that the antibody of immunized mice were able to recognize gZP3 protein. The serum of immunizedmice which was supplemented in the maturation media of cow oocyte were able to decrease the cleavagerate (p<0.05). Protein gZP3 which was supplemented in the capacitation media of the sperm also coulddecrease the cleavage rate (p<0.05). It is concluded that goat-ZP3 protein may produce cross reaction incow.

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