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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 19 Documents
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Search results for , issue "Vol 16 No 1 (2015)" : 19 Documents clear
Kualitas Spermatozoa Mencit yang Terpapar Radiasi Sinar-X Secara Berulang (SPERMATOZOA QUALITY OF MICE EXPOSED TO X-RAYS RADIATION IN REPEATED) Ni Wayan Sudatri; Ni Made Suartini; Anak Agung Sagung Alit Sukmaningsih; Dwi Ariani Yulihastuti
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

In radiology, X-ray has been used to diagnose disease and therapy. However, behind the technologybenefits provided by the radiation, the negative effects are often debated. The purpose of this study was toinvestigate the effects of repeated radiation on sperm quality mice (Mus musculus L). Thirty- two adultmale mice aged three months were divided into groups P1 (1x 200 rad), P2 (2x200 rad), P3 (3x200 rad) andcontrol irradiated with x-rays according to the experimental design . Spermatozoa quality parametersobserved were : number of spermatozoa, motility, viability and morphology of spermatozoa. The results ofthe Post Hoc LSD tests for significant differences (P>0.05) between the control and treatment showed thatthe X-ray radiation exposure to 1x200 rad, 2x200 rad, and 3x200 rad decreases the motility, viability,normal morphology and number spermatozoa produced compared with controls. This is caused by exposureto X-ray radiation causes the formation of free radicals in the body that damage sperm cells mice. Exposureto X-ray radiation repeatedly lowered the quality of spermatozoa of mice.
Viabilitas Spermatozoa Ayam Hutan Hijau dalam Pengencer Posfat Kuning Telur Ditambah Laktosa pada Penyimpanan 5oC (GREEN JUNGGLE FOWL SPERM VIABILITY IN EGG YOLK PHOSFAT DILUENTS CONTAINING LACTOSE STORAGED AT 5OC) Wayan Bebas; Desak Nyoman Dewi Indira Laksmi
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study was to determine the addition of lactose on egg yolk phosphate diluentsstored at 5°C to the plasma membrane intact, acrosome membranes intact of green jungle fowlspermatozoa, fertility and hatchability of chicken eggs. This study used completely randomized designwith three treatment groups. Treatment I (T0): egg yolk phosphate diluents only (control), treatment II(T1): egg yolk phosphate diluents + lactose 0.3%, and treatment III (T2): egg yolk phosphate diluents +lactose 0.6 %. Each group was replicated nine times. Semen was collected from eight green jungle fowl andevaluated both macroscopically and microscopically. Semen was diluted with each treatment with a singleinsemination dose (0.5 mL) with a concentration of 150 million progressively motile cells. Once diluted,semen was stored at 3-5°C for 48 hours and then was observed for the plasma membrane of intact,acrosome membrane of intact, and some semen were inseminated on local female chicken village forfertility, and hatchability eggs observation. The results showed that the addition of lactose 0.3% and 0.6%providing significant increase (P <0.05) on plasma membrane intact, acrosome membrane intact, fertilityand egg hatchability when compared to controls. However the addition of 0.3% and 0.6% lactose did notshowed a significant effect (P> 0.05). This study concluded that the addition of lactose on egg yolk phosphatediluents for storing green jungle fowl spermatozoa at 3-5oC for 48 hours can improve the plasma membraneintact, acrosome membranes intact, fertility and hatchability of chicken eggs.
Pemberian Gamal Tambahan dalam Ransum Meningkatkan Neraca Nitrogen dan Populasi Mikrob Proteolitik Rumen Sapi Bali (ENHANCEMENT PROVISION OF GLIRICIDIA SEPIUM IN DIET INCREASE NITROGEN BALANCE AND POPULATION OF RUMEN PROTEOLITIK MICROORGANISM OF BALI CATT Ni Nyoman Suryani; I Gede Mahardika; Sentana Putra; Nengah Sujaya
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research aimed to study the effect of different forage composition in diet on nitrogen balance andmicrobial population of Bali cattle. Randomized Block Design consisted of four feed treatments with 3block of weight live as replicates were used in this study. Body weight of male bali cattle used rangedbetween 181-265 kg. These four treatments based on dry matter were: A (45% elephant grass + 0% ricestraw + 15% glyricidia + 10% calliandra + 30% concentrate); B (30% elephant grass +10% rice straw + 20%glyricidia + 10% calliandra+ 30% concentrate) ; C (15% elephant grass +20% rice straw + 25% glyricidia+10% calliandra + 30% concentrate) and D (0%elephant grass + 30% rice straw + 30% glyricidia + 10%calliandra+ 30% concentrate) . Variables measured were nitrogen balance and rumen microbial population.The collected data were analyzed by analysis of variance. The result showed that nitrogen intake in cattlefed with diet C was significantly (P<0.05) higher than these in other treatments and nitrogen retention(P<0.05) was significantly higher as compared to those fed with diet A. Amylolytic and cellulolytic bacterialpopulations were not significantly different (P>0.05) among all treatments, but the population of proteolyticbacteria was found the lowest (P<0.05) in cattle fed with diet A. It can be concluded that increasedglyricidia and rice straw in the diet could increased nitrogen intake, nitrogen retention and proteolyticbacterial population.
Impacts of Pig Management and Husbandry Farmers Towards Classical Swine Fever Transmission in West Timor Indonesia (DAMPAK MANAJEMEN DAN CARA BETERNAK BABI TERHADAP PENULARAN PENYAKIT CHOLERA BABI DI TIMOR BARAT) Petrus Malo Bulu; Ian Robertson; Maria Geong
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Classical swine fever (CSF) is a serious and highly infectious viral disease of domestic pigs and wildboar, which is caused by a single stranded RNA pestivirus. A cross sectional study was carried out onsmall-holder pig farmers in West Timor, in the province of East Nusa Tenggara, Indonesia. The objectiveof this study was to describe the management, husbandry and trading practices adopted by pig farmers inWest Timor. A questionnaire survey was administered to the owners of these pigs (n = 240) to gatherinformation from farmers in order to understand management and husbandry practices in the region. Theresults of the questionnaire highlighted the lack of implementation of biosecurity measures by smallholderfarms in West Timor, which has the potential to increase the risk of their pigs to CSF, as well as toother diseases.
Deteksi Umur Pubertas Muncak (Muntiacus muntjak muntjak) Betina Berdasarkan Analisis Metabolit Estrogen dan Progesteron pada Feses (THE AGE OF PUBERTY DETECTION IN FEMALE BARKING DEER (MUNTIACUS MUNTJAC MUNTJAC) BASED ON FAECAL ESTROGEN AND PROGESTERONE A Asri Pudjirahaju; Iman Supriatna; Srihadi Agungpriyono; Muhammad Agil
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Knowledge and information about the age of puberty in muntjac (Muntiacus muntjac muntjac) isindispensable to the interests of females breeding in conservation efforts. The aims of this study were todetermine the age of puberty and age at first mated females muntjac kept in captivity through the analysisof estrogen and progesterone metabolites in feces. This study used 155 fecal samples that were collectedfrom three female muntjacs. Sample collection was began when muntjac aged three months, four monthsand six months. Total of 10-20 g fecal samples were collected every 2-4 days. Analysis of steroid hormonemetabolites was performed by using enzyme immunoassay (EIA) method with specific antibodies.Determination of the age of puberty was based on the appearance of the first time estrus and ovulation,which was indicated by the appearance of the highest estrogens secretion, on hormone metabolites profile.Hormone metabolites data then were tabulated in the average and standard deviations were presentedwith graphs and analyzed descriptively. The results showed that the age of puberty detection based onanalysis of the estrogens and progesterone metabolite in the feces can be applied in muntjac. Muntjacfemales kept in captivity flats reached puberty at age 5±1 month or 4-6 months range. It is recommendedthe first mated in the muntjac is at least after the female experienced two period of oestrous or has reachedat age of six months.
Identification and Characterization of Bovine Viral Diarrhea Virus from Indonesian Cattle (IDENTIFIKASI DAN KARAKTERISASI VIRUS BOVINE VIRAL DIARRHEA DARI SAPI INDONESIA) Muharam Saepulloh; Indrawati Sendow
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Bovine viral diarrhea virus (BVDV) is an important viral disease, which a ubiquitous pathogen ofcattle with worldwide economic importance and due to its misdiagnose with other viruses. The goal of thecurrent study was to identify and characterize of BVDV by reverse transcriptase polymerase chainreaction (RT-PCR) and followed by sequence genome analyses. Blood, feces, and semen samples werecollected from 588 selected cattle from animals suffering from diarrhea and respiratory manifestation. RTPCRresults showed that the 69 (11.74%) samples were positive to BVDV. Further molecularcharacterization was conducted only with 17 PCR positive samples. The results indicated the 17 IndonesianBVD virus isolates were belonging to the genotype-1 of BVDV (BVDV-1) based on sequence analysis anda phylogenetic relationship between Indonesian BVDV isolates and BVDV in the world. This finding is thefirst report of BVD-1 circulated in Indonesian cattle.
Waktu Optimum Biokonversi Spontan Biji Asam Guna Meningkatkan Kandungan Nilai Gizinya Sebagai Pakan Ternak Alternatif (OPTIMUM TIME OF SPONTANEOUS FERMENTATION TO NUTRIENT VALUE OF TAMARIND SEED AS ALTERNATIF FEED) Redempta Wea; Theresia Nur Indah Koni; Cytske Sabuna
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this research is to evaluate nutrition value and optimum time of spontaneous bioconversionof tamarind seed. This research was conducted using complete random design experimental methodswith five bioconversion time treatments ie R0 0 hour; R1 24 hours; R2 48 hours; R3 72 hours; and R4 96hours respectively and each treatment consist of four replications. The data were then analysis usingvariants analysis and Duncan’s test. The research variable was nutrition value of tamarind seed spontaneousbioconversion. Results showed that spontaneous bioconvertion was not effecting to dry matter, crude fiber,and crude fat but effected to ash, crude protein, and metabolism energy and the best treatment wasspontaneous bio-conversion until 72 hours and make decreasing at 96 hours treatment Based on thoseresult, for getting the best result,it is suggesting to use spontaneous bioconversion until 72 hours.
Deteksi Kerusakan DNA Spermatozoa Semen Segar dan Semen Beku Sapi Menggunakan Pewarnaan Toluidine Blue (DETECTION OF SPERM DNA DAMAGE INFRESH AND FROZEN SEMEN USING TOLUIDINE BLUE STAINING) Langgeng Priyanto; Raden Iis Arifiantini; Tuty Laswardi Yusuf
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Sperm DNA integrity is very important in the success of fertilization and embryo development.Toluidine blue (TB) is a sensitive staining to examine the chromatin structure of sperm. The aim of thisstudy was to detect the sperm DNA damage before and after freezing using TB. Semen was obtained fromeight superior bulls (two Brahman, two Ongole, two Simental and two Limosin) belong to Lembang ArtificialInsemination Centre. Semen was collected twice a week using artificial vagina, then was evaluatedmacrocopically and microscopically after collected, including sperm motility, viability, plasma membraneintegrity (MI) and acrosome intact (AI), sperm concentration, abnormality and sperm DNA integrity. Thesemen that been used in this study showed the total motility was more than 70%, sperm concentrationwas more than 1000x106, and the sperm abnormality was below 20%. The result showed that the qualityof semen after freezing processed was decreased significantly (P<0.05) on percentage of sperm motility,viability, MI and AI, whereas there was no different on DNA integrity (P>0.05). Sperm DNA integrity offresh and frozen semen were 93.91±4.77% and 92.06 ±2.41% respectively. The decrease of DNA integritywas low (1.84%) compared to motility (28.3%), viability (21.6%), MI (14.1%), and AI (11.8%). In conclusion,toluidine blue can be used to detect of DNA damage and the freezing process will not decrease the DNAintegrity.
Fraksi Heksan Daun Katuk Sebagai Obat Untuk Memperbaiki Produksi Susu, Penampilan Induk, dan Anak Tikus (HEXANE FRACTION OF SAUROPUS ANDROGYNUS LEAVES AS A MEDICINE FOR IMPROVING MILK YIELD, THE PERFORMANCE OF FEMALE, AND RAT PUPS) Agik Suprayogi; Nastiti Kusumorini; Syaprianti Evi Dame Arita
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Study of Sauropus androgynus leaves fractions as a medicine for improving milk yield, the performanceof dam, and rat pups was conducted on five groups of lactating pregnant rats. Each group consists of 16rats, namely hexane fraction (FH), ethyl acetate (Fets), water (FH2O), ethanol crude extract (EEto); andcontrol group. The administration of fractions and crude extract were executed orally through feed aftereight days of mice gestation period. The average consumption dose was 57.5 mg/day, 40 mg/day, 209 mg/day, 297.5 mg/day, and 0 mg/day, respectively. The provision of treatment for 12 days gestation periodshowed no significant effect on the average daily feed intake. The FH administration did not demonstratedifferences of growth and body weight gain of dams in comparison to the control group. Whereas theadministration of FETs, FH2O, and EE to showed the growth inhibition significantly. The FH administrationshowed gradually increase of rat pups growth and body weight gain significantly in pups 7-10 days oldcompared to the control group. However, there were signs of growth inhibition on the other groups. The FHgroup revealed a positive response on the total milk yield for 10 days of lactation, compared to the othergroups. Hexane fraction has the potency as a remedy for improving milk yield without influencing the damand rat pups performance. This study also indicates side effect on growth inhibition, especially on theadministration of ethyl acetate and water fraction, and ethanol crude extract.

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