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INDONESIA
Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
Perubahan Histopatologi Ovarium, Uterus, dan Ginjal Marmut (Cavia cobaya) yang Diberi Ekstrak Daun Binahong (Anredera cordifolia) Dwi Wijayanti; Enny Tantini Setiatin; Edy Kurnianto
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6982.782 KB) | DOI: 10.19087/jveteriner.2019.20.2.269

Abstract

Binahong (Anredera cordifolia) leaf extract has a benifficial function as an antimicrobial, heals topical wounds and accelerates the appearance of postpartum estrus. The purpose of this study was to analyze the histophatology and toxicity of ovarian, uterine, and kidney of guinea pig (Cavia cobaya) given A. cordifolia leaf extract. A total of eight females of C. cobaya were used in study, weighing ± 425 g. Treatments were given 0, 10, 50 and 90 mg of A. cordifolia leaf extract/ head, designated as T0, T1, T2 and T3, respectively. Two females were subjected for each treatment. Leaf extract of A. cordifolia was administered orally for 10 days prepartum. Observation consisted of ovarian histopathology, uterus (amount of inflammation and edema) and toxicity of kidney organs. The data obtained was analyzed by using univariate method and descriptively. The results showed there were no damage found on ovarian histology. The fastest follicular growth was found on guinea pigs treated with the dose of 50 mg of A. cardifolia/head. The inflammatory cells found in the uterus treated with 50 mg/head of A. cordifolia leaf extract were four types of inflammatory cells, at least compared to the doses of 0, 10 and 90 mg/head. Total edema in the myometrium and endometrium showed a score of 1 and 0, respectively. The dose of A. cordifolia leaf extract of 10 to 50 mg/head secured for kidney compared to a dose of 0 and 90 mg/head with glomerulonephritis and nephritis. The best treatment to accelerate follicular development, reduce inflammation and not toxic was the treatment of A. cordifolia leaf extract of 50 mg/head orally.
Isoprostan Urin Sebagai Biomarka Keracunan Etanol dan Upaya Detoksikasinya dengan Ekstrak Etanol Kulit Buah Manggis (URINE ISOPROSTANE AS TOXIC BIOMARKER OF ETHANOL AND DETOXICATION EFFORTS BY USING ETHANOL EXTRACT OF SKIN MANGOSTEEN) Ni Made Suaniti; Manuntun Manurung
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (90.427 KB)

Abstract

Isoprostane as a marker of oxidative stress which is a toxic metabolite of fatty acids, can be formedafter experiencing lipid peroxidation duo to ethanol. The aim of this study was to analyze the isoprostaneas early detection biomarker in urine of Wistar rats after ethanol consumption in sub-acute, followed byadministration of ethanol extract of rind of mangosteen fruit (EERMF). ELISA was use in this study fordiagnostic method as it can analyze the content of biological fluids even in small amount. Detection ofisoprostane compounds in the urine Wistar rats after ingestion of ethanol 20%v/v in sub acute was (0.8837± 0.0625) ng/mL decrease levels (0.4500 ± 0.0625) ng/ mL after administration EERMF 20% w/v. Isoprostancan be used as biomarker ethanol toxic and decreased level after giving EERMF.
Genetic Diversity and Molecular Phylogeny of Iranian Goats Based on Cytochrome Oxidase I (COXI) Gene Sequences (KERAGAMAN GENETIK DAN FILOGENI MOLEKULER KAMBING-KAMBING IRAN BERDASARKAN SEKUENS GEN CYTOCHROME OXIDASE I (COXI)) Reza Seyed Sharifi; Sima Savar Sofla; Hamid Reza Seyedabadi
Jurnal Veteriner Vol 18 No 4 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (92.43 KB) | DOI: 10.19087/jveteriner.2017.18.4.565

Abstract

Mitochondrial DNA has been one of the most widely used molecular markers for phylogenetic studies in animals because of its simple genomic structure. This study examines the genetic characteristic of domestic goat using sequence analysis of mitochondrial DNA Cytochrome oxidase subunit I (COXI) to identify and differentiate among three common breeds (Adani, Najdi and Markhoz) of Iran. The genomic DNA was isolated by salting out method and amplified cytochrome oxidase I gene using Polymerase Chain Reaction (PCR) method with a pair of primer. Phylogenetic trees and pairwise calculations were obtained by using Mega 6 software. A partial sequence of cytochrome oxidase I gene of Iranian goats is 1286 bp and contained four variable sites and three haplotypes. Phylogenetic analysis of haplotype in the combination with the goat from GenBank showed that Iranian goatclustered in a separate lineage. This study was found informative for establishing relationships between breeds from different parts of the world. This study may facilitate the future researchers and breeders for better understanding the genetic interactions and breed differentiation for devising future breeding and conservation strategies to preserve the rich animal genetic reservoir of the country.
Efektivitas Suplementasi Filtrat Jambu Biji dalam Pengencer AirKelapa-Kuning Telur terhadap Kualitas Semen Cair Sapi Bali (THE EFFECTIVENESS OF GUAVA FILTRATE SUPPLEMENTATION IN COCONUT WATER-EGG YOLK DILUTION ON QUALITY OF LIQUID SEMEN OF BALI CATTLE) ALoysius Marawali; Muhammad S. Abdullah; Jalaludin Jalaludin
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (110.525 KB) | DOI: 10.19087/jveteriner.2019.20.1.20

Abstract

The aim of this research was to know the effectiveness of guava filtrate supplementation in coconut water- egg yolk dilution on quality of liquid semen stored at 5oC of Bali cattle. Semen collected from a five year old Bali cattle using artificial vagina. Semen of good quality were kept in six tubes based on treatment then stored at 5oC. Treatments of the research were P0 : coconut water 80% + egg yolk 20% without guava filtrate; P1 : coconut water 80% + egg yolk 20% + 0.8% guava filtrate; P2 : coconut water 80% + egg yolk 20% + 0.9% guava filtrate; P3 : coconut water 80% + egg yolk 20% + 1.0 % guava filtrate; P4 : coconut water 80% + egg yolk 20% + 1.1 % guava filtrate and P5 : coconut water 80% + egg yolk 20% + 1.2 % guava filtrate. Each treatment was replicated 8 times making 48 experimental units. Results of the study showed that percentage mean of motility, viability, MPU, and TAU of spermatozoa after three days storage for P0 were : 42.20%, 41.85%, 39.08% and 40.90%; P1 : 50.40%, 53.89%, 52.99% and 54.67%; P2 : 54.67%, 56.97%, 54.51% and 54.36%; P3 : 17.00%, 29.96%, 29.64% and 29.64%; P4 : 23.38%, 24.64%, 21.06% and 24.45%Jurnal Veteriner Maret 2019 Vol. 20 No. 1 : 20 -29 pISSN: 1411-8327; eISSN: 2477-5665 DOI: 10.19087/jveteriner.2019.20.1.20 Terakreditasi Nasional, Dirjen Penguatan Riset dan Pengembangan, online pada http://ojs.unud.ac.id/index.php/jvet Kemenristek Dikti RI S.K. No. 36a/E/KPT/201621PENDAHULUAN Salah satu solusi yang dapat digunakan untuk pengembangan program Inseminasi buatan (IB) secara cepat dan mudah pada sapi bali adalah penggunaan semen cair. Penggunaan semen cair dapat meningkatkan kinerja IB pada sapi bali di Nusa Tenggara Timur (NTT). Keunggulan lain semen cair dapat diproduksi menggunakan bahan pengencer herbal berbasis bahan lokal dan peralatan yang sederhana serta mudah diperoleh dan tidak tergantung dengan persediaan nitrogen cair. Hasil akhir dari metabolisme spermatozoa adalah terbentuknya radikal bebas berupa derivat oksigen di antaranya adalah single1 oksigen (1O2), tripel1 oksigen (3O2), superokside anion (O2-), hidroksil radikal (OH) dan nitrit oxide (NO-) yang semuanya disebut radical oksigen species (ROS). Single1 oksigen dapat merusak ikatan rangkap pada asam lemak sehingga dapat menyebabkan kerusakan Deoxyribo Nuclead Acid (DNA) dan protein. Single1 oksigen bila bereaksi dengan asam amino histidin akan membentuk enzim yang dapat menyebabkan denaturasi protein. Kerusakan spermatozoa pada penyimpanan suhu 5%C akibat radikal bebas dan cold shock inilah merupakan penyebab utama disfungsi semen (Sharma et al., 2000). Oksidasi fosforilasi yang terganggu menyebabkan peningkatan radikal bebas dalam semen. Kadar radikal bebas yang terganggu menyebabkan peningkatan radikal bebas dalam semen. Kadar radikal bebas yang tinggi dalam sel dapat mengoksidasi lipid, protein dan DNA. Lipid membran plasma semen memiliki fosfolipid dengan kadar yang tinggi menyebabkan semen rentan terhadap radikal bebas (Sanoeka dan Kurpisz, 2004). Antioksidan bertindak mengikat asam lemak tak jenuh dan mencegah terjadinyareaksi berantai. Pada proses penyimpanan semen akan terjadi kerusakan membran plasma spermatozoa akibat terbentuknya perioksidasi lipid. Antioksidan-pemutus rantai seperti yang terkandung dalam jambu biji dapat menghambat perioksidasi lipid dalam membran melalui radical peroxyl (RO) dan alkoxyl (ROO) pengurai. Pengunaan jambu biji yang difilter dalam pengencer air kelapa kuning telur dapat menjaga kualitas spermatozoa (motilitas, keutuhan akrosom, viabilitas dan morfologi spermatozoa) semen cair sapi bali selama penyimpanan pada suhu 5%C. Dosis jambu biji yang difilter yang terbaik dalam pengencer air kelapa kuning telur, akan terbaik pula dalam mempertahankan kualitas spermatozoa sampai tujuan IB. Adapun tujuan penelitian ini adalah menguji berbagai level pemberian filtrat jambu biji (FJB) dalam pengencer air kelapa kuning telur terhadap motilitas, viabilitas, membran plasma utuh (MPU) dan tudung akrosom utuh (TAU) spermatozoa sapi bali yang disimpan pada suhu 5%C.METODE PENELITIAN Penelitian ini telah dilakukan di Laboratorium Reproduksi milik Yayasan Wiliams dan Laura yang berlokasi di Tilong, Desa Oelnasi, Kec. Kupang Tengah, Kab. Kupang, Nusa Tenggara Timur, dan berlangsung selama delapan bulan. Materi yang digunakan dalam penelitian ini adalah semen sapi bali yang ditampung dari satu ekor sapi bali jantan berumur lima tahun milik Yayasan Williams dan Laura yang telah dilatih, memiliki performans yang baik, dan organ reproduksi normal. Pakan yang diberikan adalah hijauan berupa rumput dan legum dan pemberian konsentrat secukupnya (dedak padi dan jagung giling).and P5 : 9%, 21.25%, 17.56% and 19.30%. Result of statistical analysis showed that there were a significant effect (P<0.05) between treatment on motility, viability, MPU and TAU of spermatozoa of Bali cattle till the third day of storage. It can be concluded that the supplementation of guava filtrate 0.9% in dilution of coconut water 80% - egg yolk 20% had been able to maintain motility, viability, MPU and TAU of spermatozoa of Bali cattle till the third day of storage at 5oC.
Efektivitas Sinkronisasi Estrus dan Fertilitas Spermatozoa Hasil Sexing pada Sapi Bali di Sulawesi Tenggara (EFFECTIVENESS OF ESTRUS SYNCHRONIZATION AND SPERMATOZOA FERTILITY RESULTS OF SEXING ON BALI CATTLE IN SOUTHEAST SULAWESI) Takdir Saili; La Ode Nafiu; La Ode Baa; Syam Rahadi; Astriana Napirah; Syamsuddin Syamsuddin; I Wayan Sura; Febiang Lopulalan
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (99.005 KB) | DOI: 10.19087/jveteriner.2017.18.3.353

Abstract

Estrus synchronization is one of the reproduction technology applied in the cows that aim to induce estrus of some cows to occur in the same time. In this research, all cows expressing estrus would be inseminated using sexed sperm that produced using column albumen method. Sexing sperm technology could be applied to produce the desired sex of calf. Effectivity of chilled sexed sperm to produce the desired sex of calf was evaluated in this research. Sixty three bali cows divided into 2 groups of ages (3-4 yo. and 5- 6 yo.) were used and performed synchronization using Capriglandin (PGF2a) hormone prior to application of artificial insemination with chilled sexed sperm. Variable measured were success rate of synchronization, estrus post synchronization, estrus quality, non return rate, conception rate and calving rate. The results showed that 62.90% of cows showed estrus following synchronization, estrus post synchronization occurred at 71.73 hours following synchronization, and estrus quality was 2.5%. There were 82.54% of inseminated cows was predicted to be pregnant after first insemination using chilled sexed sperm. However, only 73.02% could maintain the pregnancy up to calving. Whereas 78.26 % of newborn calf was male calf. Finally, it was concluded that PGF2a was effective to trigger estrus in bali cows, while sexed sperm still had good fertility and the sex of newborn calf was 78,26% confirmed the prediction. ABSTRAK Sinkronisasi estrus merupakan salah satu teknologi reproduksi yang diterapkan pada ternak sapi betina dengan tujuan untuk mendapatkan sejumlah ternak yang estrus secara bersamaan. Pada penelitian ini ternak yang mengalami estrus tersebut diinseminasi menggunakan spermatozoa yang telah melalui proses sexing menggunakan metode kolum albumen. Teknologi sexing spermatozoa memungkinkan untuk mengatur kelahiran anak ternak sesuai jenis kelamin yang diinginkan. Penelitian ini dilakukan untuk mengevaluasi efektivitas penggunaan semen cair hasil sexing dalam memproduksi anak sapi dengan jenis kelamin yang diinginkan. Sapi bali induk sebanyak 63 ekor yang dibagi ke dalam dua kelompok, umur 3-4 tahun dan 5-6 tahun digunakan sebagai akseptor pada penelitian ini. Sebelum inseminasi buatan (IB) dilakukan, semua sapi akseptor disinkronisasi menggunakan hormon Capriglandin (PGF2a). Variabel yang diamati adalah keberhasilan sinkronisasi, estrus pascapenyerentakan birahi, kualitas estrus, non return rate, conception rate dan calving rate. Hasil penelitian menunjukkan bahwa 62,90% sapi mengalami estrus setelah sinkronisasi dengan rataan waktu munculnya estrus 71,73 jam dan kualitas estrus 2,5. Sapi yang diprediksi bunting setelah inseminasi pertama dengan semen hasil sexing mencapai 82,54%. Jumlah sapi yang mampu mempertahankan kebuntingan hingga melahirkan hanya 73,02% dengan persentase jumlah anak sapi jantan yang dilahirkan mencapai 78,26%. Simpulan yang dapat diperoleh dari hasil penelitian ini adalah PGF2a cukup efektif merangsang munculnya estrus pada sapi bali induk dan spermatozoa hasil sexing masih mempunyai daya fertilitas yang cukup baik dengan tingkat kesesuaian jenis kelamin anak sapi yang dilahirkan mencapai 78,26%.
Identifikasi Gangguan Reproduksi pada Ovarium Sapi Potong yang Mengalami Anestrus Postpartum Panjang (IDENTIFICATION OF REPRODUCTIVE DISRUPTION ON PROLONGED POSTPARTUM ANESTRUS BEEF COW OVARIES) Bayu Rosadi; Teguh Sumarsono; Fachroerrozi Hoesni
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (84.418 KB)

Abstract

Postpartum anestrus is the major factor causing elongation of the calving interval and in consequense, lowering beef cow productivity. Anestrus condition is closey related to inactive ovary condition. The research was conducted to find out reproductive disorders in ovarian of beef cattle cow that underwent prolonged postpartum anestrus (more than 3 months). One hundred and fifty heads post partum anestrus cow were selected. The general body condition were evaluated based on Body Condition Score (BCS). The types of reproductive disruption in ovary were determined by rectal palpation method. The cows observed had 2.0 to 3.2 (2.6 ± 0.7) in BCS. The result showed that 37.68% of prolonged postparum anestrus cow had ovarian disorders. The types of ovarian disoreders detected were hypofunction (19.32%), cystic follicle (8.21%), persistent corpus luteum (4.5%), atrophy (1.93%) and partial agenesis (0.97%).
Kadar Protein Terlarut dalam Albumin Ikan Gabus (Channa striata dan Channa micropeltes) Asal Bogor SOLUBLE ROTEIN CONCENTRATION IN SNAKEHEAD FISH ALBUMIN BOGOR ORIGIN (CHANNA STRIATA AND CHANNA MICROPELTES) Rizky Alviodinasyari; Eko Sugeng Pribadi; Retno Damayanti Soejoedono
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (675.634 KB) | DOI: 10.19087/jveteriner.2019.20.3.436

Abstract

Snakehead fish is currently used by the public as a health treatment because it has a higher content of albumin protein than others. The Research aimed was determined the protein quality and comparison levels in two species of snakehead fish, Channa striata and Channa micropeltes, derived from Bogor Regency. Protein concentration level of C. micropeltes and C. striata after drying for five minutes as much as 0.830 mg/mL and 0.803 mg/mL and these concentration were higher than dried for 10 and 15 minutes. Protein concentration levels of the two species were not significantly different. The molecular weight of albumin protein from both species were 65.56 kDa to dried for five minutes, 65.08 kDa to dried for 10 minutes, and 64.91 kDa to dried for 15 minutes. The Results concluded that there were no differences in protein levels in the fish meat and skin of the two species. Drying treatment affects the molecular weight of snakehead fish albumin protein.
Tingkat Kerusakan DNA Spermatozoa Memengaruhi Profil Protein Spermatozoa pada Semen Beku Sapi Brahman (LEVEL OF SPERMATOZOA DNA DAMAGES AFFECTS SPERMATOZOA PROTEIN PROFILES IN BRAHMAN BULLS FROZEN SEMEN) Langgeng Priyanto; Agung Budiyanto; Asmarani Kusumawati; Kurniasih Kurniasih
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.73 KB) | DOI: 10.19087/jveteriner.2018.19.4.512

Abstract

The purpose of this study was to investigate the relationship between spermatozoa DNA damages with spermatozoa protein profiles of after freezing. The rate of spermatozoa DNA damages was measured by Sperm-Bos-Halomax from two Brahman bull straw samples and the spermatozoa protein was isolated by separating the upper and lower fractions of the centrifugation results. The protein profiles were then analyzed using SDS PAGE with a 12.5% separating gel concentration. The data obtained were analyzed descriptively by comparing level of spermatozoa DNA damages with spermatozoa protein profiles of upper and lower fractions. The results of the analysis showed that in the upper fractions at 37.11% level of spermatozoa damages, one protein band (29 kDa) and at 10.66% level of DNA spermatozoa damages 9 protein bands (128 kDa, 110, 91, 55, 44, 29, 27, 25 and 20 kDa) were found, respectively. Meanwhile, in the lower fractions of frozen semen, at 37.11% level of spermatozoa DNA damages 4 protein bands (105, 82, 56 and 25 kDa), and at 10.66% level of spermatozoa DNA damages 8 protein bands (109, 95, 79, 69, 50, 44, 24 and 18 kDa) were found, respectively. It can be concluded that there are differences in the spermatozoa protein profiles between different levels of spermatozoa damages.
Prevalensi dan Faktor Risiko Paramphistomiasis pada Sapi Bali di Distrik Prafi, Kabupaten Manokwari, Provinsi Papua Barat (PREVALENCE AND RISK FACTORS OF PARAMPHISTOMIASIS IN BALI CATTLE IN PRAFI DISTRICT, MANOKWARI REGENCY, WEST PAPUA PROVINCE) Purwaningsih Purwaningsih; Noviyanti Noviyanti; Rizki Pratama Putra
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (100.853 KB) | DOI: 10.19087/jveteriner.2018.19.1.91

Abstract

Paramphistomiasis is a parasiter disease caused by an Paramphistomum sp worms infection. The disease can cause a considerable decline in livestock productivity, therefore it is catagorized as one of economic affected diseases. The aims of this research were to determine the prevalence and distribution of paramphistomiasis in Bali cattle in Prafi District of Manokwari Regency, West Papua Province; and to identify some of the risk factors that associated with the paramphistomiasis prevalence. The research was done between February and March 2016. The feces samples were taken from 369 cattles perrectal were chosen by using proportional random sampling technique at village level. A total of 127 farmer respondents were interviewed. The feces samples were examined with sedimentation test to identify the existence eggs of Paramphistomum sp. based on their morphology. The data were analyzed in univariate to obtain the prevalence by dividing the number of positive sample with the number of samples inspected multiplied by 100%. Then, bivariate analysis was performed by using chi square (c2), and odds ratio (OR). The distribution of paramphistomiasis in Bali cattle that rearing in Prafi District has prevalence value in each village: Udapi Hilir, Desay, Aimasi, and Prafi Mulya was 14.74%; 12.04%; 9.18% and 1.48% respectively. While the prevalence of paramphistomiasis in bali cattle in the Prafi District was 10.03%. Factors of rearing manajemen, such as: combined shed with calve (OR=4.525) and sources of animal feed that taken from the grounds of the house and rice land (OR=4.955) showed a significant influence (P<0.05) on the risk of paramphistomiasis infection.
Modulasi Pola Fermentasi Rumen dan Emisi Gas Metana dari Penambahan Ekstrak Lerak pada Silase Gamal (MODULATION OF RUMEN FERMENTATION AND METHANE EMISSION BY LERAK EXTRACT ADDITION TO GLIRICIDIA SILAGE) Pristian Yuliana; Erika Budiarti Laconi; Anuraga Jayanegara; Suminar Setiati Achmadi; Anjas Asmara Samsudin
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (80.243 KB) | DOI: 10.19087/jveteriner.2019.20.2.202

Abstract

This study aimed to evaluate the effects of lerak fruit extract (Sapindus rarak) addition to leguminous gamal silage (Gliricidia sepium) on methane emission, rumen fermentation pattern, and rumen microbial population in vitro. In vitro rumen fermentation was designed according to a randomized complete block design with four replicates and consisted of 3 treatments, i.e. (1) gamal silage without lerak extract addition, (2) gamal silage + lerak extract 2% (w/w), and (3) gamal silage + lerak extract 4% (w/w). The measured parameters were chemical composition, saponin content, gas production, ammonia, methane, volatile fatty acid (VFA), and rumen microbial population. Results showed that the addition of 4% lerak extract increased gas production after 24 and 48 h, increased organic matter digestibility of gamal silage, increased propionate and decreased methane emission as compared to control treatment (P<0.05). Addition of lerak extract tended to reduce methanogen population and total protozoa in the rumen in vitro. Lerak extract at level 2% significantly increased ammonia concentration (P<0.05). It can be concluded that the addition of lerak extract at level 4% to gamal silage can increase gas production, organic matter digestibility and proportion of propionate, reduce methane emission, and tend to reduce metanogen population and total protozoa in the rumen in vitro.

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