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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
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Validasi Enzyme Linked Immunosorbent Assay Untuk Deteksi Antibodi Terhadap Trypanosoma evansi (VALIDATION OF AN ENZYME LINKED IMMUNOSORBENT ASSAY FOR DETECTION OF ANTIBODY ANTI-TRYPANOSOMA EVANSI) Didik Tulus Subekti; Ichwan Yuniarto
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Surra is a disease caused by Trypanosoma evansi (T.evansi) infection. Indonesia was known as an endemic country for Surra. Development of Enzyme Linked Immunosorbent Assay (ELISA) is very useful for early detection of Surra and for routine surveillance of animals infected by T.evansi. In Indonesia the development of ELISA kit for the detection of antibodies to T.evansi was reported in 1999 and 2003. Unfortunately, the assay performance was still unsatisfactory because it had a low sensitivity (81-89%). Therefore, the development of the validated ELISA kit which is applied nationally in Indonesia is very important. Validation was performed to evaluate the performance of ELISA kit, especially their diagnostic sensitivity, specificity and accuracy. Validation was also carried out to evaluate the method and criteria for determining definitive and universal cut-off values. The results of the validation showed a high sensitivity and specificity that are 95.8% and 98.2% using receiver operating curve (ROC) analysis. Diagnostic accuracy, sensitivity and specificity for advanced validation using universal cut-off values are 96.15%; 93.75% and 98.21% respectively. The best criteria for determining the universal cut-off value is MSD ? 2SD, S / P? 40, SNP ? 25. The cut-off value that can be applied to produce better assay performance based on evaluation by two different operators is MSD ? 2SD.
Karakterisasi Molekuler Gen Penyandi SodC Pasteurella multocida yang Diisolasi dari Kerbau Asal Nusa Tenggara Timur (MOLECULAR CHARACTERIZATION OF SodC ENCODING GENES PASTEURELLA MULTOCIDA ISOLATED FROM BUFFALO OF EAST NUSA TENGGARA ORIGIN) Ayang Mahindra; Hani Plumeriastuti; Didik Handijatno
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Pasteurella multocida type B:2 is a bacteria which causing the disease was called Septicemia Epizooticae in ruminants, expecially cattle and buffalo. The aims of this research to determine the encoding gene SodC of Pastuerella multocida type B:2. This isolate of Pasteurella multocida type B:2 was obtained from the BBVet Denpasar, Bali that originally from East Nusa Tenggara (NTT) Indonesia. The sample was grown on Blood agar (BA). Separated colonies were grossly observed identified by macroscopicly (transparent, round and sweet-smelling) and microscopicly identified (bipolar and Gram negative), Biochemical test included Triple Sugar Iron Agar (TSIA), Urease, Simmnons Citrate Agar (SCA), Sulphid Indol Motility (SIM), Sugars Test (Sucrose, Manitol, Maltose, Glucose and Lactose). This Isolate had been test using Polymerase Chain Reaction (PCR), the result of PCR test if Pasteurella multocida had band in 235bp. This research was continued with Sequencing test to reveal the nucleotides sequence of the encoding SodC gene in Pasteurella multocida and also the homology from Genbank or NCBI. Result of Blasting in NCBI revealed that this encoding gene had similar nucleotides sequence with Pasteurella multocida strain 4407 (97%), Chinese 9N Pasteurella multocida strain (98%), Chinese Pasteurella multocida strain BS168 (97%), Pasteurella multocida strain EB168 China (97 %), Pasteurella multocida subsp. multocida strain CIRMBP-0884 France (97%), and Pasteurella multocida subsp. multocida strain RCAD0259 China (96%).
Anatomi Makroskopis dan Analisis Dinamika Profil Darah pada Proses Penyembuhan Luka pada Kulit Landak Jawa (Hystrix javanica) (MACROSCOPIC ANATOMY AND HEMATOLOGICAL CHANGES DURING WOUND HEALING PROCESS IN THE SKIN OF THE JAVA PORCUPINE (Hystrix javanica)) Srihadi Agungpriyono; Andhika Yudha Prawira; Huda Salahudin Darusman; Wartika Rosa Farida; Savitri Novelina
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

In Sunda Porcupine, wound healing occurred in a relative quick period, minimum scar formation, and without infection even though the wound is large and severe. The physiology which involved blood function as the systemic component is important in wound healing. This study aimed to acknowledged the macroscopic anatomy of the clinical signs, the role of blood component, and blood biochemistry in wound healing in Sunda porcupine (Hystrix javanica). The study used 6 adults of Sunda porcupine (3 males and 3 females). Stadium 3 wounds (full-thickness wound) were applied in thoracodorsal and lumbosacral region, approximately 10-11 cm2. Clinical signs observations were performed in day 0, 3, 7, 10, 14, 30, and 40, while blood collection were conducted in day 0, 7, 14, 21, 30, and 40 post wounding. The results of this study showed that wound closure occurred in 30-40 days post wounding with thoracodorsal wound closed faster than that in lumbosacral. Blood profile alteration during wound healing showed that wound healing occurred well. The increasing profile of erythrocytes and platelets during wound healing showed that these two components might become important of internal factor in wound closure, whereas the alteration of leucocytes showed no prolonged inflammation in wound healing in Sunda porcupine. The alteration of liver enzyme and renal function showed a fluctuation however the wound healing occurred well, whereas the decreased blood glucose level indicated no prolonged wound healing to chronic stage. These results give the information of clinical view and internal factors which contribute to the wound healing in the skin of Sunda porcupine.
Analisis Hemagglutinin Virus Flu Burung (Avian Influenza) Subtipe H5N1 Asal Pasar Unggas Hidup di Jakarta Timur 2014 (HEMAGGLUNIN ANALYSES OF AVIAN INFLUENZA VIRUSES SUBTYPE H5N1 ISOLATED FROM LIVE BIRD MARKETS IN EAST JAKARTA 2014) Risza Hartawan; Ni Luh Putu Indi Dharmayanti
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Hemagglutinin is a surface glycoprotein of the influenza virus encoded by segment 4, which has the main function to attach to the host cell receptor. This glycoprotein is one of the most important markers for determining viral pathogenicity and virulence. Furthermore, hemagglutinin also has experienced massive mutations since its epitopes exposed to the external environment resulting in various variants in the field. The main objective of this study was to characterize molecularly the avian influenza virus subtype H5N1 isolated from live bird markets in East Jakarta in 2014 using a bioinformatics approach. As a result, this study identified the circulation of the highly pathogenic virus from both clade 2.1.3 and 2.3.2 with several mutations in the hemagglutinin sequence. These viruses still displayed a high affinity for the avian receptor. Subsequently, the prediction of the 3D structural model of hemagglutinin demonstrated differences in the protein conformations, especially in the HA2 domain and cleavage site. These molecular predictions require further investigation studies, especially in vivo experiments with animal models.
Uji Kelarutan Kristal Kalsium Oksalat Urin Tikus Sprague Dawley setelah Pemberian Eliksir Ekstrak Etanol Daun Pelawan (SOLUBILITY TEST OF CALCIUM OXALATE CRYSTAL IN SPRAGUE DAWLEY RAT URINE AFTER GIVING ETHANOL EXTRACT OF PELAWAN LEAVES) Rahmawati Januar; Ietje Wientarsih; Sus Derthi Widhyari
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Traditional medicine is an herbal medicine used from generation to generation by people in Indonesian. Pelawan leaves is believed can dissolve crystals in the urine that can be the initial cause of kidney stone formation. This study aimed to evaluate the potential of elixir of the ethanol extract of pelawan leaves as antilithiasis in rat was given ethylene glycol and ammonium chloride as inducer. Thirty male rat Sprague dawley was divided in five different groups (n=6). The control group (K1), ethylene glycol (EG) and ammonium chloride (AC) as inducer (K2), inducer and Batugin 5,4 mg (K3); inducer and the elixir of the ethanol extract ofpelawan leaves (EEEDP) 100 mg/kg body weight (K4) and 200 mg/kg body weight (K5). The treatments were done in 28 days with urines observated at day 14 and day 28. The result showed that pelawan contain flavonoid, tannin, saponin and steroid. The result showed that treatment groups dissolved calcium oxalate crystals. Observated at day 14, K3 dissolved calcium oxalate crystals with lowest number, while at day 28, K4 dissolved calcium oxalate crystals with lowest number. A dose of 100 mg/kg body weight in the K4 group effectively dissolves the calcium oxalate crystals.
Hematological Profiles of Green Sea Turtle (Chelonia mydas) Rehabilitated at Turtle Conservation and Education Center, Serangan Island, Denpasar, Bali Leoni Arifah Agustina; Nyoman Sadra Dharmawan; Ida Bagus Windia Adnyana
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Threat of extinction results in the increasing sea turtle conservation efforts through scientific research in recent years. During rehabilitation process, the non-invasive blood analysis is considered to be highly valuable to determine sea turtle’s health. The objective of this study was to assess the hematological profiles of green sea turtles (Chelonia mydas) under rehabilitation at Turtle Conservation and Education Center (TCEC) Serangan Island, Denpasar, Bali. Three mililiters of peripheral blood from healthy green sea turtles were collected from cervical dorsal sinuses and kept in heparinized vacutainer tube before proceeded with hematological evaluation. PCV was determined by microhematocrit method. Diff count and thrombocyte percentage were determined using Giemsa-stained blood smear under the microscope. RBC and WBC total count were determined using hemocytometer. Hemoglobin (Hb) value was determined using Sahli method. Each hematological values were then analyzed using descriptive analysis. The correlation between body condition index (BCI) and hematological values were analyzed using Spearman’s rank correlation test. The mean values evaluated was 7.28 g/dL in Hb, 31.35% in PCV, 0.67 x 106/?L in total RBC count, 479.37 fl in MCV, 111.38 pg in MCH, 23.42 g/dL in MCHC, 4.01 x 103/?L in total WBC count, 1.20 x 103/?L in lymphocyte, 0.21 x 103/?L in monocyte, 0.10 x 103/?L in eosinophil, 2.48 x 103/?L in heterophil and 0.01 x 103/?L in basophil. No significant correlation was found between the BCI and hematological profiles evaluated.
Penambahan Water Additive Kimchi Dapat Mencegah Infeksi Alami Flu Burung (Avian Influenza) pada Ayam Pedaging (THE SUPLEMENTATION OF WATER ADDITIVE KIMCHI COULD PREVENT NATURAL INFECTION OF AVIAN INFLUENZA IN BROILER) I Putu Cahyadi Putra; Raden Wasito; Hastari Wuryastuty
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Subclinical infection of avian influenza (AI) in day old chick (DOC) has potential to interfere the success of vaccination programs. Therefore, an alternative ways to increase avian innate immunity in preventing the infection is necessary. The purpose of this study was to evaluate the effect of adding water additive (KimchiStock®) in preventing the occurrence of AI natural infection in DOC raising in commercial broiler farms. Fifty-five broiler DOCs, Cobb strains were used as experimental animals. Before the experiment begin, five DOCs were randomly selected, sacrificed and the lung were taken out for polymerase chain reaction (PCR) analysis on the matrix region. Fifty remaining DOCs were randomly divided into two: K1 (control) and K2 (treated) groups. K2 group were given 0.2% water additive (KimchiStock®) 5 days/ week for five weeks. Every week for five consecutive weeks, five chicken from each group were randomly selected for sera and lung collection. Sera samples were analyzed for antibody titer detection using haemagglutination inhibition technique. The results showed that there is no significant different in antibody titer between control and treated group. The majority of the antibody titer in this experiment were under 23. At week 4 the antibody titer of one chicken in treated group was 23 but no clinical sign was observed. Molecularly, there were no positive result detected from the lung of five DOCs at the beginning of the experiment and control group. While in the K2, positive result were detected on week 1 and 2 but without any clinical sign. Based on the research results could be concluded that adding 0.2% water additive KimchiStock® could prevent AI natural infection starting from week 3.
Gambaran Kelistrikan Jantung Anak Babi pada Kondisi Renjatan dengan Resusitasi Hipervolemik Menggunakan Cairan Kristaloid Natrium Chlorida 0,9% Gunanti Gunanti; Soesatyoratih Soesatyoratih; Antonius H Pudjiadi; Melpa Susanti Purba; Galih Satria Kusumanto
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study aimed to determine the effect of resuscitation and hypervolemic conditions on shocked piglet’s heart electrical system. Animal models that were used in this study are 5 healthy castrated piglets. The piglet’s blood were withdrawn until the mean arterial pressure dropped about 20 % from the baseline condition to make a hypovolemic shock condition. When the piglets reached the hypovolemic shock state, fluid resuscitation using crystalloid (NaCl 0.9 %) as much as 40 ml/kg were given using a 50 ml syringe through the jugular vein. ECG examination were performed before surgery and when it reached the hypervolemic state after being resuscitated. The ECG results indicate no apparent change in cardiac performance due to the absence of ECG waveform changes from waveforms during normal conditions. In conclusion, crystalloid fluid resuscitation (NaCl 0.9 %) until hypervolemic condition does not interfere with cardiac performance.
Kadar Krioprotektan Gliserol dan Dimethylsulfoxide Terbaik pada Pengencer Astaxanthin Fosfat Kuning Telur Bebek Terhadap Kualitas Semen Beku Babi Wayan Bebas; Wayan Gorda
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study to examine the addition of cryoprotectant glycerol and dimethylsulfoxide (DMSO) in astaxanthin phosphate duck egg yolk on the quality of frozen boar semen. Each of the cryoprotectants distinguished its concentration ie : 4%, 6%, 8%, 10%, and 12% for freezing of semen. Semen was collected from one Landrace boar then performed macroscopically and microscopically. Semen was divided into 10 tubes each diluted with the duck egg yolk astaxanthin phosphate extender diluent then stored at 20oC for 2 hours, centrifugation for 15 minutes (2000 rpm) then taken the pellet with 1 ml of supernatant. The pellets were diluted with astaxanthin phosphate duck egg yolk extender containing various concentration (4, 6, 8, 10, and 12%). Semen was packed with 0.5 mL straw and performed equilibration for 2 hours (4oC). do aeration above liquid N2 vapor for 10 minutes, then dipped in liquid N2. Semen was stored in liquid nitrogen container for 24 hours , and then quality of spermatozoa was evaluated post thawing. The results of the study showed that addition of cryoprotectan glycerol and DMSO were no difference significant (p>0,05) on the quality of frozen semen, but cryoprotectant concentrations (4%, 6%, 8%, 10%, and 12%) significantly affected (p <0.05) the quality of frozen semen. Glycerol with a concentration of 4% produces the best quality frozen semen, whereas DMSO with a concentration of 6% produces the best quality frozen semen.
Biokimia Darah Hati dan Ginjal Setelah Implan Wire SS316L dan Wire Alternatif (BLOOD BIOCHEMISTRY OF LIVER AND KIDNEY AFTER IMPLANT WIRE SS316L AND ALTERNATIVE) Erwin Erwin; Rusli Rusli; Amiruddin Amiruddin; Etriwati Etriwati; Muhammad Isa; Abdul Harris; Yula Astuti
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The blood biochemistry changes of liver and kidney are indicative of disorder in these organs. The objective of our experimental approach was to examine the level serum glutamic pyruvic transaminase (SGPT), serum glutamic oxaloacetic transaminase (SGOT), blood urea nitrogen (BUN) dan creatinine after implant wire SS316L and alternative. Ten rats wistar strain aged 2-3-month-old, weighing 250-300 g were divide into two groups. Group I (G-I) implanted wire SS316L and group II (G-II) implanted alternative wire in os femur with each had a diameter of 0,5 mm. Blood sample was taken from sinus orbitalis on day 0 before implant, on day seven, 14, 21, 42 and 84 after implant. The results showed an increase in levels SGPT, SGOT, BUN and creatinine in the first few weeks after implantation. The levels of SGPT, SGOT, BUN and creatinine decreased again in the last week of observation with significant differences between the two treatment groups (P<0.05). The implant wire SS316L and alternative does not cause increased levels SGPT, SGOT, BUN dan creatinine for long time. Thus, it can be concluded that alternative wire has potential as internal fixation in fracture of small animal.

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