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Jurnal Biologi Udayana
Published by Universitas Udayana
ISSN : 14105292     EISSN : 25992856     DOI : https://doi.org/10.24843/jbiounud
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Jurnal Biologi Udayana (p-ISSN 1410-5292 | e-ISSN 2599-2856 | DOI 10.24843/jbiounud) managed by the Department of Biology, Udayana University, published in two formats namely print and online regularly twice a year (June and December).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 262 Documents
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PENGARUH STEROID ANABOLIK METHANDIENONE TERHADAP KUANTITAS SPERMATOZOA TIKUS PUTIH (Rattus norvegicus) Nurul Marfu'ah; I Wayan Kasa; Sagung Chandra Yowani
Jurnal Biologi Udayana Vol 18 No 1 (2014): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh steroid anabolik methandienone terhadap kuantitas spermatozoa tikus putih (Rattus norvegicus). Pemeriksaan kuantitas spermatozoa dilakukan pada testis dan epididimis kauda. Testis dibuat sebagai sediaan preparat dengan metode parafin dan pewarnaan Hematoxylin-Eosin kemudian dilakukan penghitungan jumlah spermatogonia, spermatosit, dan spermatid. Penghitungan jumlah spermatozoa epididimis kauda dilakukan berdasarkan prosedur WHO dalam Syamrizal (1995). Hasil penelitian menunjukkan bahwa rerata kuantitas spermatozoa antara kelompok kontrol dan kelompok perlakuan secara statistik tidak berbeda nyata (P>0,05). Meskipun demikian, rerata kuantitas spermatozoa menunjukkan kecenderungan menurun. Hal ini ditunjukkan oleh rerata jumlah spermatogonia yang mengalami kecenderungan menurun pada dosis 6 dan 12 mg/kg bb. Rerata jumlah spermatosit antar kelompok perlakuan juga menunjukkan kecenderungan menurun. Begitu pula rerata jumlah spermatid dan spermatozoa juga menunjukkan kecenderungan menurun.
Diversity of parasitoid Hymenoptera in banana germplasm plantation Yogyakarta Ichsan Luqmana Indra Putra
Jurnal Biologi Udayana Vol 23 No 1 (2019): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (557.289 KB) | DOI: 10.24843/JBIOUNUD.2019.v23.i01.p04

Abstract

Banana is one of the fruits in Indonesia. The development of its production in Indonesia has increased. However, there are several factors that can reduce the production, one of which is pests. Pests on bananas can be controlled with natural enemies, one of which is parasitoid. The study aimed to calculate the diversity and abundance of parasitoids in the Yogyakarta banana germplasm plantation. The research was carried out in an area of approximately 2 Ha from April to June 2018 with sampling taken once a week. Sampling method used was in the form of transect. The determination of the transect point is determined as far as 500m between sampling points. At each sampling point carried out 2 sampling methods. Direct method by taking pests on 3 banana plants at each point and indirect with insect net and yellow pan traps. The results found that Shannon-Wiener Index value was 3.29. 22 Hymenoptera parasitoid families were found with the highest morphospecies were superfamily Chalcidoidea and the highest number of individuals were Scelionidae.
ALDEHID DEHIDROGENASE DALAM TIKUS WISTAR SEBAGAI BIOMARKER AWAL KONSUMSI ALKOHOL SECARA AKUT NI MADE SUANITI; A.A.GEDE SUDEWA DJELANTIK; I KETUT SUASTIKA; I NYOMAN MANTIK ASTAWA
Jurnal Biologi Udayana Vol 15 No 1 (2011): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Research on oral consumption of alcohol on rat Wistar was done to examine level of aldehyde dehydrogenase (ALDH) in rat serum. The research design was true randomized experimental post test only control group design. This research was conducted in two stages: first, eight rats were treated with 5% alcohol continuously for 1 week and as control aquadest was given to eight rats. The second stage was determination of ALDH levels. Wistar rat serum were taken after 6 hours and 24 hours of 5% alcohol consumption. The levels of ALDH increase by 117.15% after 6 hours of 5% alcohol consumption, while after 24 hours the levels of ALDH increase by 108.14%. ALDH levels in serum rat Wistar can be used as early biomarker of acute alcohol consumption.
BLOOD GLUCOSE LEVEL AND PANCREAS HISTOLOGICAL SECTION OF MICE ( Mus musculus L.) INDUCED BY ALLOXAN AFTER TREATMENT OF Curcuma mangga Val. RHIZOME EXTRACT Madihah Madihah; Fitriani Alfina; Yetti Yusri Gani
Jurnal Biologi Udayana Vol 20 No 2 (2016): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Herbal-based drug development for diabetes mellitus continues to grow in order to find alternatives of theuse of synthetic drugs which is relatively expensive. The present study examined the potency of temu mangga(Curcuma mangga Val.) rhizome extract in decreasing blood glucose levels and repairing histological damage ofpancreas endocrine gland in male mice (Mus musculus L.) Swiss-Webster that has been induced by alloxan. Theexperimental method with 5 treatments and 5 replications were used. The dose of alloxan was 200 mg/kg bw,while the dose of temu mangga extract were 100, 200, 400 and 800 mg/kg bw. The measured parameters werethe body weight, fasting blood glucose levels by using blood glucose tolerance test, and the percentage of pancreatic? cells that undergo necrosis. Data were analyzed by ANOVA with 95% confidence level and continued withDuncan’s multiple range tests. The results showed no difference on body weight of test animals in all treatments.The reduction percentage of fasting blood glucose levels from temu mangga rhizome extract by dosage of 400mg/kg bw (48.712%) was significantly different from the treatment of alloxan (0.588%) (p<0.05). The percentageof ? cells that undergo necrosis from temu mangga rhizome by dosages of 200, 400, and 800 mg/kg bw weresignificantly different with alloxan (22.75±3.68 %) (p<0.05). In conclusion, temu mangga rhizome extract bydosage of 400 mg/kg was optimum to decrease blood glucose levels and repair the pancreas histological damagein mice that were induced by alloxan.
POTENSI MELANOTUS SP. DALAM MENDEGRADASI LIGNIN NUNIK SULISTINAH
Jurnal Biologi Udayana Vol 12 No 1 (2008): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Ten isolates of fungus were isolated from oil palm stem at oil palm plantation in Medan All of them were tested its abilities to degrade lignin. The results showed that one of them was able to grow on ligninase media and the fungi has the ability to degrade ligin. The fungi is identified as Melanotus sp.
TOKSISITY OF DETERGENT AND ARTIFICIAL TEXTIL COLOR TO WATER STRIDER (Gerris marginatus) I K. Putra Juliantara; Ni Luh Watiniasih; I Wayan Kasa
Jurnal Biologi Udayana Vol 19 No 1 (2015): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Kualitas perairan tawar dapat dipengaruhi oleh masuknya polutan, misalnya detergen dan pewarna kain sintetis, sehingga berpengaruh terhadap kelangsungan hidup organisme air seperti serangga akuatik (anggang-anggang). Tujuan dari penelitian ini adalah untuk mengetahui toksisitas detergen dan pewarna kain sintetis terhadap angganganggang.Penelitian ini terdiri dari dua faktor, yaitu detergen dan pewarna kain sintetis. Faktor detergen terdiri dari empat konsentrasi yaitu konsentrasi 0, 3, 6 dan 9 ppm, demikian juga dengan pewarna kain sintetis terdiri dari empat konsentrasi yaitu konsentrasi 0, 15, 30 dan 45 ppm. Penelitian ini menggunakan rancangan perlakuan faktorial 4x4 dan rancangan percobaan RAK (Rancangan Acak Kelompok). Hasil penelitian menunjukkan bahwa detergen berpengaruh negatif terhadap daya tahan hidup anggang-anggang dalam waktu 24 jam (P<0,05), demikian juga pengaruh pewarna kain sistesis (P<0,05). Akan tetapi, tidak ada pengaruhinteraksi dari detergen dan pewarna kain sintetis terhadap kematian anggang-anggang (P>0,05). Selain itu, ukuran tubuh memberikan pengaruh yang nyata terhadap waktu kematian anggang-anggang (P<0,05). Dari hasil penelitian dapat disimpulkan bahwa detergen dan pewarna kain sintetis meningkatkan jumlah kematian anggang-anggang dalam 24 jam, namun tidak ada efek interaksi antar kedua perlakuan.
THE EFFECT OF DIFFERENT ALTITUDE TO THE POLLEN TYPES THAT TRIGONA COLLECTED I PUTU NARKA EKA PRATAMA; NI LUH WATINIASIH; I KETUT GINANTRA
Jurnal Biologi Udayana Vol 22 No 1 (2018): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (535.911 KB) | DOI: 10.24843/JBIOUNUD.2018.v22.i01.p06

Abstract

Trigona is a stingless bee, which has been found in tropical and sub-tropical regions. Fifty species have been indentified in South East Asian region, but few studies have been conducted in Indonesia. Plant species commonly differ in different altitudes, due to the climatic effect, therefore will affect the availability of food source for Trigona. This study aimed to investigate the effect of different altitudes to the pollen types collected and used by Trigona as food resources and the distance of their foraging. Samples of Trigona were collected from 3 locations: Location I was in Mawang and Taro Villages at Gianyar Regency, Location II was in Tua Village at Tabanan Regency and Location III was in Ngis Village at Karangasem Regency. The altitudes of those three locations were 750.87 m, 493.007 m and 147.15 m above sea levels respectively. Pollen samples were processed using the acetolysis methods in the Plant Structure and Development Laboratory, Department of Biology, Udayana University and pollen identification was referred to the book of “Pollen Morphology and Plant Taxonomy” (Erdtman, 1972). The results showed that in some cases, the type pollen collected by Trigona differ in different altitude. Pollen of Allamanda cathartica L was collected by the bee at Gianyar and Karangasem Regencies. Pollen of Capsicum frutescens L. and Euphorbia milii were collected by the bee at Tabanan and Karangasem Regency, while pollen Neomarica longifolia was found in bees in all three locations. The average distances of foraging of this Trigona bee was 147.15 m at Gianyar, 162.21 m at Tabanan and 53.61 m at Karangasem.
SUPPRESSION ABILITY OF CRUDE EXTRACT DERIVED FROM MARINE BIOTA AGAINST FUSARIUM OXYSPORUM F.SP. VANILLAE I Ketut Suada; Ni Wayan Suniti
Jurnal Biologi Udayana Vol 14 No 1 (2010): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

The objective of this research was to investigate suppression ability of marine biota extracts against Fusarium oxysporum f.sp. vanillae of vanilla stem rot. Samples were collected at intertidal zones and in the depth of 1-7 m from seven beaches in Bali. Screening of active compounds of biota extracts were conducted using inhibition zone of well diffusion method on Potato Dextrose Agar (PDA). The extract was tested in-vitro in PDA medium using completely randomized design with three replicates. The methanolic extract of Aglaophenia sp. was able to suppress the growth of F. oxysporum f.sp. vanillae effectively, with minimum inhibition concentration (MIC) of 0.05 %. The extract inhibited colony growth diameter and total mycelial dry weight.
PENGARUH PEMBERIAN PUPUK ORGANIK PADA MEDIA TANAH YANG MENGANDUNG TIMBAL (Pb) TERHADAP PERTUMBUHAN KANGKUNG DARAT (Ipomoea reptans Poir.) Listiatie Budi Utami; Ulfah Rachmawati
Jurnal Biologi Udayana Vol 20 No 1 (2016): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Kangkung darat (Ipomoea reptans Poir.) merupakan tanaman hiperakumulator  logam timbal (Pb), padahal kangkung darat banyak dikonsumsi oleh masyarakat. Penelitian ini bertujuan untuk mengetahui pertumbuhan dan kandungan timbal (Pb) kangkung darat pada berbagai dosis pupuk organik; serta untuk mengetahui dosis pupuk organik yang paling efektif untuk meningkatkan pertumbuhan dan menurunkan kandungan timbal (Pb) dalam kangkung darat. Penelitian dilakukan secara Rancangan Acak Lengkap (RAL) dengan perlakuan pemberianpupuk organik dengan dosis 0 gram, 50 gram, 100 gram, 150 gram, 200 gram, dan 250 gram dalam 2 kg tanah dari TPA Piyungan, Bantul. Setiap perlakuan diulang 4 kali dan dilakukan selama 4 minggu. Pengamatan meliputi tinggi tanaman, jumlah daun, panjang daun, panjang akar, dan berat basah tanaman. Pada minggu ke-4, dilakukan pengukuran kadar timbal (Pb) dalam daun. Dilakukan uji ANOVA dan BNT 5%. Hasil penelitian menunjukkan bahwa pemberian pupuk organik dapat meningkatkan pertumbuhan tanaman kangkung darat. Dosis yang paling efektif untuk pertumbuhan tanaman kangkung darat adalah 200 gram dalam 2 kg tanah. Pemberian pupuk organik tidak dapat menurunkan kandungan Pb dalam tanaman kangkung darat.
IDENTIFICATION OF MICROBES ANTAGONISTIC AGAINST Fusarium oxysporum ISOLATED FROM RHIZOSPHERE ZONE OF WATERMELON Anak Agung Ngurah Nara-Kusuma; Yan Ramona; Meitini Wahyuni Proborini
Jurnal Biologi Udayana Vol 22 No 2 (2018): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (558.834 KB) | DOI: 10.24843/JBIOUNUD.2018.v22.i02.p04

Abstract

This research was aimed to isolate and identify microbes which antagonistic against Fusarium oxysporum, the causative agent of vascular wilt in watermelon plants. The antagonistic microbes were isolated from soil samples collected from rhizosphere of watermelon farm located at west Sanur village, South Denpasar, Bali. Isolation of fungi and bacteria were conducted on potato dextrose agar medium (PDA) and nutrient agar medium (NA), respectively. Fungal isolates were then observed under light microscope for its morphological characteristics before identification using a reference book. Bacterial isolates were characterized using various tests, such as gram stain reaction, existence of endospores, catalase reaction, and ability to ferment various sugars. Their characteristics were then compared with those described in a reference book. Two fungal species (Trichoderma harzianum and Trichoderma viride) and two bacterial antagonists (Pseudomonas sp. and Bacillus sp.) were found to have potential to be developed as biocontrol agents to inhibit the growth of F. oxysporum.

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