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Jurnal Biologi Udayana
Published by Universitas Udayana
ISSN : 14105292     EISSN : 25992856     DOI : https://doi.org/10.24843/jbiounud
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Jurnal Biologi Udayana (p-ISSN 1410-5292 | e-ISSN 2599-2856 | DOI 10.24843/jbiounud) managed by the Department of Biology, Udayana University, published in two formats namely print and online regularly twice a year (June and December).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 262 Documents
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GAMBARAN MIKROSKOPIS GINJAL TIKUS PUTIH (Rattus sp) JANTAN DEWASA SETELAH PEMBERIAN ETANOL KRONIS Ayu Kasmita Dewi; Ni Made Rai Suarni; Ni Made Suaniti
Jurnal Biologi Udayana Vol 17 No 2 (2013): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Telah dilakukan penelitian dengan tujuan untuk mengetahui gambaran mikroskopis ginjal tikus putih (Rattus sp.) jantan dewasa setelah pemberian etanol kronis selama 48 hari. Delapan belas ekor tikus jantan dewasa dibagi ke dalam tiga kelompok secara acak. Pemberian alkohol diberikan secara oral (gavage) dengan dosis alkohol 10% dan 20% sebanyak 1ml/hari. Pada kelompok kontrol hanya diberikan aquades sebanyak 1ml/hari. Hasil penelitian menunjukkan bahwa alkohol 10% menyebabkan kerusakan pada ginjal seperti vakuolisasi lumen tubulus, pendarahan, dan infiltrasi sel radang yang bersifat fokal. Alkohol 20% menyebabkan kerusakan pada ginjal seperti infiltrasi sel radang bersifat multifokal (menyebar), pendarahan, vakuolisasi lumen tubulus, akumulasi sel debris dalam lumen tubulus, dan karyomegali. Variabel perubahan histopatologi ginjal yang diamati kemudian ditentukan tingkat kerusakannya dengan metode skor. Hasil yang diperoleh dianalisis dengan menggunakan uji ANOVA (analysis of variance) selanjutnya diuji dengan uji LSD. Dapat disimpulkan bahwa semakin meningkat konsentrasi alkohol yang diberikan secara oral (gavage) pada tikus putih jantan dewasa dapat meningkatkan kerusakan pada ginjal.
OPTIMASI AMPLIFIKASI DNA MENGGUNAKAN METODE PCR (Polymerase Chain Reaction) PADA IKAN KARANG ANGGOTA FAMILI Pseudochromidae (DOTTYBACK) UNTUK IDENTIFIKASI SPESIES SECARA MOLEKULAR Ni Putu Dian Pertiwi; i G.N.K Mahardika; Ni Luh Watiniasih
Jurnal Biologi Udayana Vol 19 No 2 (2015): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Polymerase Chain Reaction (PCR) merupakan salah satu metode yang digunakan dalam identifikasi suatu organisme. Identifikasi secara molekular menggunakan metode berbasis PCR perlu dilakukan pada ikan karang Famili Pseudochromidae karena ikan ini mempunyai variasi morfologi warna yang sangat tinggi dan menyulitkan identifikasi morfologi. Metode amplifikasi DNA untuk seluruh spesies ikan anggota famili ini belum banyak dilakukan. Penelitian ini bertujuan untuk mengetahui kondisi optimal untuk amplifikasi DNA menggunakan metode PCR (Polymerase Chain Reaction) pada 25 spesies ikan karang anggota famili Pseudochromidae, yang sebelumnya telah diidentifikasi secara morfologi.Amplifikasi dilakukan pada tiga loki DNA mitokondria, yaitu 16S rRNA, control region dan cytochrome oxidase I (COI). Hasil penelitian menunjukkan kondisi optimum amplifikasi tidak berhubungan dengan perbedaan spesies. Modifikasi amplifikasi dapat dilakukan dengan penambahan volume template DNA dan BSA 1X serta penggantiantemperatur annealing; sedangkan pergantian reagen tidak memberikan pengaruh yang signifikan. Penggunaan primer depan CRK untuk amplifikasi lokus control region juga memberikan hasil yang lebih baik.
THE GENETIC VARIATIONS OF INDIGENOUS BALINESE (BALI MULA) COMMUNITY AT SEMBIRAN VILLAGE USING MICROSATELITE DNA MARKERS Made Çri Dwitiari
Jurnal Biologi Udayana Vol 16 No 1 (2012): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

This study aimed to investigate the genetic variations of the indigenous Balinese (Bali Mula) population in Sembiran village utilizing four DNA microsatellite: D2S1338, D3S1358, D5S818 and D13S317. Amplified DNA analyzed with PCR SuperMix Kit, Invitrogen. Total alleles found in Bali Mula population at Sembiran village were 19 alleles. Genetic variations were determined using heterozygosity formulae, and the mean of heterozygosity of the four loci was 0,6145. Keywords: DNA Amplified, DNA microsatellite, heterozygosity.
VARIASI BENTUK WAJAH LAKI-LAKI DAN PEREMPUAN DARI DESA PANGLIPURAN, KABUPATEN BANGLI, PROVINSI BALI AGUNG EKO CAHYA KUSUMA PUTRA; NI NYOMAN WIRASITI; IRIANI SETYAWATI
Jurnal Biologi Udayana Vol 21 No 2 (2017): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (704.277 KB) | DOI: 10.24843/JBIOUNUD.2017.vol21.i02.p03

Abstract

This research determined male and female face type of the adults in Panglipuran adults. The analysis of face types Geometric Morphometric Technique in thin-plate-splin (tps) program. Adults female and 14 male aged 25-35 years old were photographed for both frontal and lateral faces with a digital camera Canon EOS 60D. Landmark data was processed by photo processor program tpsDig version 2.04, tpsSuper version 1.13, tpsRelw version 1.42, tpsSplin 1.20 and both of ape and gee package of R Program version 3.2.5. The average of male’s face shape type in Panglipuran village was ellipse and round for female’s. We found 2 frontal and 2 lateral for male’s face variation, we also found 3 frontal and 3 lateral for female’s. Frontal face of male and female that showed high variation included right subaurale, left subaurale, right gonion, left gonion, and gnathion. Lateral face of male and female that showed high variation were trichion, frontozygomaticus, superciliare, superaurale, pogonion, glabella, the minimum point of the curve below earlobe, gonion and gnathion.
MUDWHELKS (GASTROPODA: POTAMIDIDAE) FROM MANGROVES OF UJUNG KULON NATIONAL PARK, BANTEN NOVA MUJIONO
Jurnal Biologi Udayana Vol 13 No 2 (2009): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

A study of Potamididae snail from the mangrove ecosystem in Ujung Kulon National Park was conducted, regarding to its diversity, shell characters and habitat characters. This study was carried out in 2008 during drought and rainy season. At the eight stations observed, seven species and four genera of Potamididae snail were identified. Compared with previous study, this study added new information from obtaining one genus (Cerithidea) of three species (Cerithidea weyersi, C. quadrata and Cerithideopsilla alata)
PENGARUH 2,4-D TERHADAP HIPOKOTIL DAN KOTILEDON KEDELAI VARIETAS SLAMET YANG DITUMBUHKAN SECARA IN VITRO Iman Budisantoso; Lucky Prayoga
Jurnal Biologi Udayana Vol 16 No 2 (2012): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Tujuan yang akan dicapai dalam penelitian ini adalah mengetahui pengaruh 2,4-D terhadap pertumbuhan dan perkembangan hipokotil dan kotiledon kedelai varietas Slamet dalam kultur in vitro. Penelitian dilakukan secara eksperimental dengan rancangan percobaan Rancangan Acak Lengkap (RAL), perlakuan yang dicobakan adalah larutan 2,4-D yang terdiri dari 4 level konsentrasi yaitu 0 mg/L; 20 mg/L; 40 mg/L dan 60 mg/L dan masing-masing perlakuan diulang 4 kali. Parameter perkembangan eksplant yang diamati meliputi prosentase pertumbuhan melalui pembentukan kalus maupun proses embriogenesis, waktu tumbuh kalus, berat basah kalus dan jenis kalus yang terbentuk. Hasil penelitian menunjukkan bahwa eksplant hipokotil maupun kotiledon kedelai varietas Slamet yang ditumbuhkan dalam media MS dengan perlakuan 2,4-D 20-60 mg/L tidak menghasilkan kalus maupun tanaman melalui proses embriogenesis, eksplant berwarna coklat dan mati. Setelah perlakuan konsentrasi hormon diubah menjadi 0, 5, 10 dan 15 mg/L, kalus tumbuh pada eksplant hipokotil dengan perlakuan 2,4-D 5 mg/L, sedangkan eksplant kotiledon kalus tumbuh pada media dengan perlakuan 2,4-D 15 µM.
Keberadaan bakteri patogen pada sampel pangan jajanan anak sekolah dasar di Pulau Sapeken, Sumenep, Jawa Timur Ivani Dayanara; Retno Kawuri; Dwi Ariani Yulihastuti
Jurnal Biologi Udayana Vol 23 No 2 (2019): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (762.33 KB) | DOI: 10.24843/JBIOUNUD.2019.v23.i02.p04

Abstract

School children snacks are food that is found and routinely consumed by children in the school environment.Good and healthy food must be free from hazardous and toxic materials, such as microbial contamination,chemicals and other materials. The consumed food must meet the microbial contamination requirementsbased on SNI (Standar Nasional Indonesia) safety testing. Pathogenic bacteria still a serious problem invarious countries including Indonesia, since they are capable of causing foodborne diseases. Sapeken Islandis located in Sapeken Village, Sapeken District, Sumenep Regency, East Java Province. The purpose of thestudy was to determine the presence of pathogenic bacteria in samples of snacks for school children onSapeken Island. The method used was the Most Probable Number (MPN) for Escherichia coli, Test Numberof Bacillus sp., Test Number of Staphyllococcus sp., Identification of Salmonella, and Total Plate Count(TPC). The test results obtained in the MPN of Escherichia coli included SD M (9.9 ± 3.8) MPN / g, SD 5(8.68 ± 2.73) MPN / g, SD IT (5.08 ± 0.66) MPN / g, and SD 4 (4.26 ± 2.44) MPN / g exceed the SNI limit.JURNAL BIOLOGI UDAYANA 23(2): 60-71 P ISSN: 1410-5292 E ISSN: 2599-285661The results of JPT E. coli in snack empek-empek at SD I and SD 2, and JPT E. coli ojek in all elementaryschools is <3 MPN / g. The results of the empek-empek and ojek samples in all elementary schools for alltests with the parameter number Bacillus sp. is <1 x 103, the number of Staphylococcus sp. is <1 x 102,Salmonella identification is negative, TPC is <1 x 105 CFU / g. The conclusion of the study is snack forschool children (empek-empek) and ojek on Sapeken Island does not contain contamination of pathogenicbacteria Escherichia coli, Bacillus sp., Staphylococcus sp., Salmonella identification, and TPC, except forthe JPT E. coli test on empek empek in SD M, SD 5, SD IT and SD 4 that exceed SNI limits.
PERKEMBANGAN LATISIFER PADA KULTUR KALUS CATHARANTHUS ROSEUS (L) G. DON YANG DIINDUKSI DENGAN KOMBINASI ZAT PENGATUR TUMBUH KINETIN + NAA NI NYOMAN DARSINI
Jurnal Biologi Udayana Vol 15 No 2 (2011): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

The development of laticifer on callus culture of Catharanthus roseus (L) G Don in Zenk medium supplemented with combination of plant growth regulator kinetin + NAA was studied. The explants were taken from the second folium from shoot apex. Development of laticifer was observed using descriptive analysis method for callus anatomy and percentage of laticifer was observed during 4–14 weeks of callus development. The percentage of laticifer was determined by counting the average number of the laticifer and the average number of surrounding cells in every optical field of few under light microscope. The results showed that early development of laticifer which was induced with plant regulator growth kinetin + NAA was found in the 9 weeks old callus. The laticifer has specific characteristics i.e. thicker cell wall and longer cell than sorounding cell. Elongated laticifer was observed at 12 weeks old callus. The highest percentage of laticifer on callus C. roseus induced with combination of kinetin and NAA was found in 12 weeks old callus i.e. 0,12%. At 13 and 14 weeks old callus, the anatomy of laticifer was similar to that at 12 weeks old callus, but the percentage was lower.
KEANEKARAGAMAN SPESIES ULAR DI DESA PERING, KECAMATAN BLAHBATUH, KABUPATEN GIANYAR, BALI I GEDE MADE ARIUS HADY BUDIADA
Jurnal Biologi Udayana Vol 21 No 1 (2017): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (209.471 KB) | DOI: 10.24843/JBIOUNUD.2017.vol21.i01.p02

Abstract

Snakes are cold-blooded reptiles belong to the Order Squamata, Suborder Serpentes. Snakes often found in lowlands, highlands, trees, soil, water and swamps. People in the Pering village especially in Banjar Perang Sada have less knowledge about venomous and non-venomous snakes. Therefore, it is important to investigate the diversity of snakes in the Pering village, Blahbatuh, Gianyar. This study was conducted on 28 January – 4 February 2015. Data collection was performed by direct observation along the potential snake habitats in two plots rice field near residential area and near the Pura Beji. Data collection was performed during night time from at 7:00 to 11:00 PM with sampling focused on Banjar Perang Sada. Individual snake found was cought and identified in situ. This research found 9 species of snakes namely, Ahaetulla prasina, Boiga cynodon, Dendrelaphis pictus, Pareas carinatus, Ptyas korros, Rhabdophis chrysargos, Xenochrophis piscator, Bungarus fasciatus, and Trimeresurus insularis, the most commonly found was Dendrelaphis pictus.
PERILAKU DAN JENIS PAKAN ORANGUTAN KALIMANTAN (PONGO PYGMAEUS LINNAEUS, 1760) DL KALIMANTAN PURWO KUNCORO; SUDARYANTO -; LUH Puru ESWARYANTI KUSUMA YUNI
Jurnal Biologi Udayana Vol 12 No 2 (2008): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Data collection was conducted using focal animal instantaneous. The daily activity data were group based on their ethogram and was adopting "The Standard of Orangutan's collection" from Morrogh-Bernard et al. (2002). The research collected 739 hours 29 minutes of dawn to dusk observations. The daily activity was determined by activity proportion. Rehabilitated Orangutans was found to have higher proportion of social activity (79,29%) than the one of self-action activity (23,71%). The daily activity proportions consisted of 10,44% (females) and 14,13% (males) for movement, 29,01% (females) and 30,66% (males) for resting, 12,34% (females) and 4,90% (males) for social activities, 3,36% (females) and 2,49% (males) for self activities, and also 44,85% (females) and 47,82% (males) for foraging with 96 plants species and 1 termite species as food sources.

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