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All Journal International Journal of Public Health Science (IJPHS) Jurnal Riset Kesehatan Jurnal Penelitian Kesehatan Suara Forikes Health Notions THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Jurnal Penelitian Pendidikan IPA (JPPIPA) Manuju : Malahayati Nursing Journal Journals of Ners Community JURNAL PENELITIAN KESEHATAN JURNAL ANALIS LABORATORIUM MEDIK Medicra (Journal of Medical Laboratory Science/Technology) JOURNAL OF COMMUNITY MEDICINE AND PUBLIC HEALTH RESEARCH Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) Jurnal EduHealth Sewagati Analis Kesehatan Sains (e-Journal) Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat Jurnal Sains Student Research Jurnal Laboratorium Khatulistiwa Jurnal Pengabdian Kesehatan Beguai Jejama Frontiers in Community Service and Empowerment
., Pestariati
Poltekkes Kemenkes Surabaya
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Electrical & Electronics Engineering Engineering Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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Journal : Health Notions

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Solid Media (Lowenstein Jensen) and Liquid Media (Mycobacteria Growth Indicator Tube) Usage Against Mycobacterium tuberculosis Culture in Sputum Suspect Tuberculosis Pestariati Pestariati; Meilinda Rachmawati
Health Notions Vol 5, No 6 (2021): June
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn50607

Abstract

Tuberculosis examination using Lowenstein Jensen (LJ) media, requires an incubation time of 8 weeks after inoculation. The objective of this studi to compare result media Mycobacterium tuberculosis proliferation on solid media (LJ) and liquid media (MGIT) on sputum of suspected TB. The liquid culture method was Mycobacteria Growth Indicator Tube (MGIT). This method was fully automatic and non-radiometric. MGIT contains 7.0 ml of modified Middlebrook 7H9 broth. Culture results will come out in 42 days if there was no bacterial growth. Comparative research on 30 sputum suspects of TB were examined at the Surabaya Health Laboratory Center on 03 January to 20 March 2020. The sputum samples were examined through the stages of decontamination, homogenization, concentration and inoculated on LJ media and MGIT media and incubated at 370C. Identification of the colonies that grew with ZN staining and MPT 64 immunochromatography test. The results of the Wilcoxon Signed Rank Test statistical test obtained p-value of 0.157 (p> 0.05). It can be concluded that there is no difference in the culture results of Mycobacterium tuberculosis on solid media (Lowenstein Jensen) and liquid media (Mycobacteria Growth Indicator Tube). Keywords: Mycobacterium tuberculosis; Lowenstein Jensen (LJ);Mycobacteria Growth Indicator Tube (MGIT)
Sorgum Agar (Sorghum bicolor) as Substitute Nutrient Agar Media for Cultivation Escherichia coli Pestariati Pestariati; Mufidah Mufidah
Health Notions Vol 5, No 07 (2021): July
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn50706

Abstract

Media (nutrient agar) is a medium that is often used for bacteriological examinations and the price is relatively more expensive, Sorghum bicolor materials that are easy to get and cheap. Sorghum contains carbohydrates and protein which are suitable for manufacturing agar nutrients. This research was an experimental quantitative analysis laboratory. The research used Sorghum bicolor flour as carbohydrates and proteins in nutrient agar media. This study used variations in the mass of sorghum flour 2.50 grams, 3.75 grams, 5.00 grams, and 6.25 grams. The average number of bacterial colonies at mass variations was 2.50 grams, 3.75 grams, 5.00 grams, and 6.25 grams, namely 70.75 × 1013 CFU / mL, 60.75 × 1013 CFU / mL, 49, 25 × 1013 CFU / mL, and 42 × 1013 CFU / mL with significant differences in the value of p = 0.000 or
Co-Authors A'yunin, Zahratin Nisa' Anggraini, Anita Dwi Anindya, Lokawanti Al Fahna Anita Dwi Anggraini Anita Dwi Anggraini Ariana, Novi Armelia Gitasari Nurhasanah Asryadin, Asryadin Ayu Puspitasari, Ayu Christ Kartika Rahayuningsih Dawami, Tamymatur Roudhatud Diah Titik Mutiarawati Edy Haryanto Endah Astuti, Sri Sulami Endarini, Lully Hanni Evy Diah Woelansari Gufron, Gusti Naila Rachmawati Handayati, Anik I Gusti Bagus Wiksuana I Nyoman Krisna Wicaksana Ida Lestari Iis Rofiatul Ana Indrayani, Salsabila Anggia Istanto , Wisnu Istanto, Wisnu Juliana Christyaningsih Krihariyani, Dwi Kumala, Nyna Rachma Kusumaningrum, Debby Liliek Soetjiatie Luthfi Rusyadi Mamik Mamik Mamik Mamik Meilinda Rachmawati Minarti Minarti Minarti Minarti Mufidah Mufidah Muhammad Sholakhuddin Al-Ayubi Museyaroh, Museyaroh Mutiarawati, Diah Titik Nurhasanah, Armelia Gitasari Nyna Rachma Kumala Retno Sasongkowati Rina Idawati Sania, Zahrotus Sasongkowati, Retno Sri Sulami Endah Astuti Sri Utami Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suliati Suliati Suliati Suliati Syamsul Arifin Syamsul Arifin Verdiani, Intan Wicaksana, I Nyoman Krisna Wisnu Istanto Wisnu Istanto Wisnu, Istanto Yuniati, Nilasari Indah