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All Journal JURNAL ILMIAH PLATAX AGRICOLA BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Acta Aquatica: Aquatic Sciences Journal Bioscientist : Jurnal Ilmiah Biologi Nekton ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua
Saleky, Dandi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Social Sciences Veterinary Other

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Identifikasi Molekuler Udang Penaeid dengan Pendekatan DNA Barcoding Saleky, Dandi; Dailami, Muhammad; Manan, Jemmy; Manumpil, Abraham Welliam
Nekton Vol 2 No 2 (2022): October
Publisher : Politeknik Negeri Sambas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (467.987 KB) | DOI: 10.47767/nekton.v2i2.395

Abstract

Penaeid Shrimp (Crustacea: Decapoda) is an important fishery commodity in human life both in terms of economy, ecology, and nutrition. Penaeid shrimp are widely distributed and abundant on the coast of Merauke and become one of the leading commodities in the fisheries sector. The identification problem in the Penaeid shrimp group is a problem that still often occurs today. Identification problems are often not carried out because they are considered unimportant even though they can be important information for conservation and sustainable management. This study was conducted to identify the Penaeid Shrimp from Payum Beach, Merauke Regency, Papua by using a DNA barcoding approach with the Cytochrome Oxidase I Gene. The results of the analysis showed that the species of specimens was Mierspenaeopsis sculptilis (Heller, 1862) with a similarity level of 97.38%. Genetic distance and level of DNA similarity are very influential in phylogenetic reconstruction. Identification with DNA barcoding has succeeded in identifying species quickly and accurately and can be used to confirm previously identified species based on morphological characters.
Identifikasi Jenis Gastropoda Conus spp. di Perairan Pesisir Manokwari Karubuy, Randy Imanuel Sandy; Manan, Jemmy; Manangkalangi, Emmanuel; Sembel, Luky; Saleky, Dandi
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.7688

Abstract

Gastropods Conus spp. is a macrozoobenthic organism that is abundant in coastal waters and is consumed by coastal communities. Conus is a carnivorous organism that lives permanently at the bottom of the waters and has the property of accumulating pollutant materials such as pesticides, carbonates and heavy metals into the body's tissues through the food chain. The aim of this study was to identify the gastropods species Conus spp. from the Manokwari Coastal Waters. The available research method is quantitative descriptive research. The target species in this study were gastropods of the genus Conus spp. collected from the Manokwari Coastal Waters. A total of 27 individuals from 6 species of Conus spp. collected from 3 observation stations. Gastropods Conus spp. 6 species were found, namely: 1) Conus leopardus; 2) Conus imperialis; 3) Conus eburneus; 4) Conus virgo; 5) Conus planorbis, and 6) Conus lividus. The air quality parameters measured were within the appropriate range for the Gastropods Conus spp.
Pertumbuhan dan Mortalitas Ikan Kuro (Eleutheronema tetradactylum) yang Didaratkan di Pelabuhan Perikanan Nusantara Kabupaten Merauke Kusmita, Ranny; Sunarni, Sunarni; Saleky, Dandi
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9407

Abstract

This research aimed to determine the size structure, length-weight relationship, growth, and mortality of kuro fish (Eleutheronema tetradactylum) that landed on Nusantara Fishery Harbour, Merauke District. The samples were taken off in August-October 2022 at Nusantara Fishery Harbour, Merauke District. The metusedsing was a purposive sampling method. The measured data was the total length and weight body of kuro fish. The size structure and length-weight relationship of kuro fish were analyzed using Microsoft Excel. Meanwhile, the growth and mortality were analyzed using the FISAT II application. The result analysis shows that the range of E. tetradactylum total length landed on Nusantara Fishery Harbour is 430 – 1030 mm with a weight range of 92 – 3613 grams. The length-weight relationship of E. tetradactylum has the allometric negative growth pattern with equation W = 0,0016L2,1167 in August, W = 0,0017L2,1134 in September, and W = 0,0017L2,1077 in October. The analysis of E. tetradactylum’s growth parameter shows the value L∞ = 1053 mm, K = 0,51/year, and t₀ = -0,1201 year. The result mortality analysis of E. tetradactylum shows the total mortality (Z) was 0,78/ year, natural mortality was 0,43/year, fishing mortality (F) was 0,35/year, and exploitation rate was 0,45/year.
Identifikasi Genetik Ikan Gabus Asal Merauke dengan Menggunakan Fragmen Gen Sitokrom Oksidase Sub Unit I Dailami, Muhammad; Yuniarti, Ating; Saleky, Dandi; Toha, Abdul Hamid A.
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11446

Abstract

Efforts to cultivate snakehead fish (Chanidae) still experience many problems, especially the availability of high-quality fry. To improve the quality of fry, improving the quality of broodstock is important. Genetic factors play an important role in the selection and breeding of snakehead fish parents. Therefore, it is necessary to explore sources of superior parents with good genetic characteristics. To achieve this goal requires studies from various fields such as ecology, biology, and genetics. Genetic studies can be started by examining the identity and relationships of snakehead fish from Merauke, as a first step in finding a source of germplasm that has high diversity. The aim of this research is to analyze the cytochrome oxidase subunit 1 (COI) gene fragment of snakehead fish from Merauke, Papua. DNA isolation was carried out using the Promega kit with standard protocols, amplification of the COI gene with primers F2 and R2 with the Go Taq Green amplification kit. A total of 653 base pairs of the COI gene fragment were obtained from the sequencing process, with a nucleotide composition of T (29.9%), C (28.8%), A (24.0%), G (17.3%). BLAST results on the NCBI database show that this sequence has 100% similarity to the Channa striata sequence from Papua with sequence ID OQ405388.1. This shows that the identification results of the snakehead fish from Merauke are Channa striata. Phylogenetic tree analysis also showed consistent results, namely the formation of a clade that was the same as the Channa striata fish group derived from GenBank data.
Kajian Molekuler Ikan Sembilang (Neosilurus sp.) di Perairan Rawa Padi Kabupaten Merauke Saleky, Dandi; Siruru, Meyani Anjelita; Sunarni, Sunarni; Pangaribuan, Rosa Delima
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13387

Abstract

Sembilang is one of the fish that is abundant in Merauke mainland waters. Genetic research needs to be done to prevent the decline of catfish population. DNA barcoding is a taxonomic method that uses short genetic markers in an organism's DNA to identify species. This research aims to identify the Sembilang fish species using the Cytochrome Oxidase I gene. This study is an exploratory survey research. Sample collection was carried out in the waters of Padi Swamp, Merauke Regency. The mitochondrial cytochrome oxidase I (COI) gene was amplified using the Polymerase Chain Reaction (PCR) method with FISH-BCL and FISH-BCH primers. The results of analysis using DNA barcoding revealed that the samples collected were the Sembilang species (Neosilurus ater) with a DNA sequence length of 660 bp. Phylogenetic reconstruction using the Neighbor-Joining method with the 2-Parameter Kimura Model shows sequence grouping based on similarity and genetic distance between species.
Filogenetik Ikan Kuro (Eleutheronema sp.) di Perairan Pesisir Merauke, Papua Selatan Saleky, Dandi; Situmorang, Ferdinand C.; Welliken K., Marius A.; Pangaribuan, Rosa D.
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.13685

Abstract

This research aims to identify kuro fish (Eleutheronema sp.)  collected from the coastal waters of Merauke. Cytochrome Oxidase Subunit I (COI) Gene was amplified by the Polymerase Chain Reaction (PCR) method, using primers FISH F1 (5’-TCA ACC AAC CAC AAA GAC ATT GGC AC-3’) and FISH R1. (5’-TAG ACT TCT GGG TGG CCA AAG AAT CA -3’), while DNA data analysis uses Mega 11 software. Results research shows that DNA sequence obtained were 675 base pairs long with the identification result being Eleutheronema tetradactylum with a similarity level of 99.54 and 99.85% for each sample. Based on phylogenetic analysis and genetic distance, the two DNA sequences analyzed form the same clade with identification results with a genetic distance of 0.001. Analysis of genetic diversity found 2 haplotypes from the two samples. Both the Sample Have a small genetic distance because THEY are collected from the same waters DNA barcoding and phylogenetics can be used to identify species for management and conservation of species.
Komunitas fauna makrozoobentos di kawasan reboisasi mangrove Kepulauan Seribu: faktor lingkungan, distribusi, ekologi komunitas, pola sebaran dan hubungannya Syahrial, Syahrial; Larasati, Chandrika Eka; Saleky, Dandi; Isma, Muhammad Fauzan
Acta Aquatica: Aquatic Sciences Journal Acta Aquatica: Aquatic Sciences Journal, Vol. 7: No. 2 (October, 2020)
Publisher : Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/aa.v7i2.2456

Abstract

AbstrakMangrove sangat penting bagi kehidupan biota pesisir dan laut, dimana telah mengalami kerusakan dan juga telah dilakukan rehabilitasi. Kajian komunitas fauna makrozoobentos di kawasan reboisasi mangrove Kepulauan Seribu berdasarkan faktor lingkungan, distribusi, ekologi komunitas, pola sebaran dan hubungannya telah dilakukan pada bulan Maret 2014. Hal ini bertujuan untuk mengetahui faktor lingkungan, distribusi, ekologi komunitas, pola sebaran serta hubungannya. Faktor lingkungan diukur secara in situ, sedangkan fauna makrozoobentosnya dikumpulkan dengan transek garis dan plot. Kemudian ekologi komunitas fauna makrozoobentosnya dilihat berdasarkan indeks keanekaragaman, dominansi maupun keseragaman, sedangkan pola penyebarannya dianalisis dengan indeks morisita serta hubungan antara faktor lingkungan, ekologi komunitas dan pola penyebarannya dianalisis menggunakan regresi linier sederhana. Faktor lingkungan yang diukur tidak begitu berbeda dan juga tidak melebihi baku mutu. Sebanyak 6 spesies dengan 3 kelompok fauna makrozoobentos telah ditemukan di kawasan reboisasi mangrove Kepulauan Seribu. Kemudian indeks keanekaragaman maupun keseragamannya tergolong rendah, dominansinya tergolong tinggi dan pola penyebarannya tergolong seragam (uniform). Selain itu, hubungan antara faktor lingkungan (suhu, salinitas dan DO perairan) dengan indeks keanekaragaman maupun keseragaman berkorelasi positif, sedangkan hubungan antara faktor lingkungan dengan indeks dominansinya berkorelasi negatif. Kemudian korelasi antara faktor lingkungan dengan pola penyebaran berkorelasi positif (membentuk kelompok) dan korelasi antara ekologi komunitas (keanekaragaman dan keseragaman) dengan pola penyebarannya juga berkorelasi positif serta membentuk kelompok.Kata kunci: fauna makrozoobentos; reboisasi mangrove; faktor lingkungan; distribusi; ekologi komunitas; pola sebaran; Kepulauan SeribuAbstractMangrove ecosystems are essential for the life of coastal and marine biota, which have been damaged and have been reforested. Study macrozoobenthos fauna community in mangrove reforestation area of Kepulauan Seribu was conducted in March 2014. This aimed to assess the relationship between environmental factors, distribution, ecology, and distribution patterns of the macrozoobenthos fauna community. The environmental factors were measured in situ, and macrozoobenthos was collected using line and plot transect. The ecology of macrozoobenthos fauna was analyzed based on diversity, uniformity, and dominance index. Distribution patterns were analyzed using Morisita index, and their relationships were analyzed using linear regression. Six species from 3 groups were found in this area. Diversity and uniformity index was classified low, dominance index was high, and the distribution patterns were relatively uniform. In addition, the relationship between the environmental factors (temperature, salinity, and DO) with diversity and uniformity index were positively correlated, and relationship between the environmental factors with dominance index were negatively correlated. The correlation between environmental factors with distribution patterns were positive (form groups), and the correlation between community ecology with distribution patterns were positive.Keywords: community ecology; distribution; Kepulauan Seribu; mangrove reforestation; macrozoobenthos fauna
Gastropoda test family of Neritidae as bioindicator to health status of mangrove forest Pulau Tunda Serang Banten, Indonesia Anggraini, Rika; Syahrial, Syahrial; Karlina, Ita; Mariati, Wandesi; Saleky, Dandi; Leni, Yusyam
Acta Aquatica: Aquatic Sciences Journal Acta Aquatica: Jurnal Ilmu Perairan, Vol. 8: No. 1 (April 2021)
Publisher : Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/aa.v8i1.3829

Abstract

Uji gastropoda famili Neritidae terhadap habitatnya di ekosistem mangrove dilakukan di dua stasiun pengamatan di Pulau Tunda Serang Banten pada Januari 2014. Penelitian ini bertujuan untuk mengkaji dan menganalisis gastropoda famili Neritidae terhadap kesehatan hutan mangrove Pulau Tunda Serang Banten. . Pengumpulan data kondisi gastropoda famili Neritidae dilakukan dengan menggunakan plot berukuran 1 x 1 m dan dipasang pada plot transek vegetasi mangrove berukuran 10 x 10 m, dimana transek garis dan plot vegetasi mangrove ditarik dari titik acuan (tegakan mangrove bagian luar) dan tegak lurus terhadap garis pantai ke daratan.Kemudian keanekaragaman, dominansi, dan keseragaman gastropoda famili Neritidae dan hutan bakau Pulau Tunda Serang Banten dianalisis menggunakan indeks keanekaragaman Shannon-Weaver. Indeks dominasi Simpson dan indeks keseragaman Shannon-Weaver. Sedangkan hubungan kerapatan gastropoda famili Neritidae dan kerapatan hutan bakau Pulau Tunda Serang Banten dilakukan dengan menggunakan regresi linier sederhana. Hasil penelitian menunjukkan bahwa kerapatan gastropoda famili Neritidae lebih tinggi dan lebih banyak ditemukan di mangrove kerapatan tinggi. Kemudian keanekaragaman dan dominasi gastropoda famili Neritidae rendah, sedangkan keseragamannya dalam keadaan seimbang.Selain itu, penelitian ini juga mengungkapkan bahwa semakin tinggi kerapatan hutan mangrove maka kerapatan gastropoda famili Neritidae juga semakin tinggi, sehingga gastropoda famili Neritidae dapat digunakan sebagai bioindikator dalam menentukan kesehatan hutan mangrove.Sedangkan hubungan kerapatan gastropoda famili Neritidae dan kerapatan hutan mangrove Pulau Tunda Serang Banten dilakukan dengan menggunakan regresi linier sederhana. Hasil penelitian menunjukkan bahwa kerapatan gastropoda famili Neritidae lebih tinggi dan lebih banyak spesies ditemukan pada mangrove kerapatan tinggi. Kemudian keanekaragaman dan dominasi gastropoda famili Neritidae rendah, sedangkan keseragamannya dalam keadaan seimbang. Selain itu, penelitian ini juga mengungkapkan bahwa semakin tinggi kerapatan hutan mangrove maka kerapatan gastropoda famili Neritidae juga semakin tinggi, sehingga gastropoda famili Neritidae dapat digunakan sebagai bioindikator dalam menentukan kesehatan hutan mangrove. Sedangkan hubungan kerapatan gastropoda famili Neritidae dan kerapatan hutan mangrove Pulau Tunda Serang Banten dilakukan dengan menggunakan regresi linier sederhana. Hasil penelitian menunjukkan bahwa kerapatan gastropoda famili Neritidae lebih tinggi dan lebih banyak spesies ditemukan pada mangrove kerapatan tinggi. Kemudian keanekaragaman dan dominasi gastropoda famili Neritidae rendah, sedangkan keseragamannya dalam keadaan seimbang. Selain itu, penelitian ini juga mengungkapkan bahwa semakin tinggi kerapatan hutan mangrove maka kerapatan gastropoda famili Neritidae juga semakin tinggi, sehingga gastropoda famili Neritidae dapat digunakan sebagai bioindikator dalam menentukan kesehatan hutan mangrove. Kemudian keanekaragaman dan dominasi gastropoda famili Neritidae rendah, sedangkan keseragamannya dalam keadaan seimbang. Selain itu, penelitian ini juga mengungkapkan bahwa semakin tinggi kerapatan hutan mangrove maka kerapatan gastropoda famili Neritidae juga semakin tinggi, sehingga gastropoda famili Neritidae dapat digunakan sebagai bioindikator dalam menentukan kesehatan hutan mangrove. Kemudian keanekaragaman dan dominasi gastropoda famili Neritidae rendah, sedangkan keseragamannya dalam keadaan seimbang. Selain itu, penelitian ini juga mengungkapkan bahwa semakin tinggi kerapatan hutan mangrove maka kerapatan gastropoda famili Neritidae juga semakin tinggi, sehingga gastropoda famili Neritidae dapat digunakan sebagai bioindikator dalam menentukan kesehatan hutan mangrove.
Co-Authors Abdul Hamid A. Toha Asaribab, Pilipus Y. Ating Yuniarti Chandrika Eka Larasati, Chandrika Eka Emmanuel Manangkalangi Isma, Isma Isma, Muhammad Fauzan Ita Karlina Jefri Sembiring Karubuy, Randy Imanuel Sandy Kusmita, Ranny Leni, Yusyam Manan, Jemmy Manumpil, Abraham W. Manumpil, Abraham Welliam Mariati, Wandesi Mendes, Johanna Merly, Sendy Lely Muhammad Dailami, Muhammad Pangaribuan, Rosa D. Pangaribuan, Rosa Delima Rika Anggraini RR. Ella Evrita Hestiandari Sembel, Luky Sianturi, Reny Simon P.O Leatemia, Simon P.O Siruru, Meyani Anjelita Siti Jubaidah, Siti Situmorang, Ferdinand C. Sunarni Sunarni SYAHRIAL SYAHRIAL Thomas Frans Pattiasina Welikken, Marius A. Welliken K., Marius A. Weremba, Efraim