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Variasi Kosentrasi Cuka Apel terhadap Daya Hambat Pertumbuhan Escherichia coli dan Staphylococcus aureus Rasmi, Kadek Anidia; Aryasa, I Wayan Tanjung; Laksmita, Ayu Saka
Bali International Scientific Forum Vol. 4 No. 1 (2023): Bali International Scientific Forum
Publisher : Bali International University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34063/bisf.v4i1.293

Abstract

Background: Apple cider vinegar is a fermentation of apple cider which contains various compounds that are beneficial for health. Such can be useful as an antibacterial due to the content of acetic acid, gallic acid, and catechins. Purpose: This study aims to determine the effect of variations in the concentration of apple cider vinegar and the most effective concentration of apple cider vinegar in inhibiting the growth of Escherichia coli and Staphylococcus aureus in vitro. Method: The method used in this study is a quasi experimental with disc diffusion for antibacterial testing. In this study, four concentrations of apple cider vinegar were used, that are 100%, 75%, 50%, and 25%. Result: The results of the antibacterial activity test on Escherichia coli showed the average diameter of the inhibition zone, i.e. 12.33 ± 0.12 mm (strong category) for concentration 100%, 10.57 ± 0.20 mm (strong category) for concentration 75%, 6.52 ± 0.22 mm (medium category) for concentration 50%, and 2.56 ± 0.18 mm (weak category) for concentration 25%. Whereas Staphylococcus aureus showed the average diameter of the inhibition, i.e. 14,56 ± 0,46 mm (strong category) for concentration 100%, 12,60 ± 0,07 mm (strong category) for concentration 75%, 8,62 ± 0,04 mm (medium category) for concentration 50%, and 4,54 ± 0,12 mm (weak category) for concentration 25%. Conclusion: Apple cider vinegar concentrations of 75% and 100% were effective in inhibiting the growth of the two test bacteria because they showed inhibition with strong category. Keywords: apple cider vinegar, Escherichia coli, Staphylococcus aureus.
Deskripsi Tingkat Pengetahuan Masyarakat Denpasar terhadap Obesitas Lebih Rentan Terinfeksi COVID-19 Laksmita, Saka
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 2 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i2.17752

Abstract

COVID-19 is a virus that can spread through direct contact, indirect contact, or close contact with an infected person. This situation makes a person vulnerable to disease. Good knowledge about COVID-19 and healthy living behaviours as an effort to prevent transmission of COVID-19 are essential to implement. The aim is to determine the description of the understanding of the people of Denpasar City related to obesity being more susceptible to COVID-19 infection. This type of research is descriptive by conducting a survey of 400 people in Denpasar City through purposive sampling. This study used a questionnaire as a data collection tool and the data were analyzed univariately. Based on the characteristics of the respondents, it was found that most respondents were in the age range of 21-30 years. For the latest education, most of the respondents are high school / equivalent and university graduates with a percentage of 53.25% and 46% respectively. In terms of occupation, it was found that most of the respondents consisted of students with a percentage of 70.5% and the least were in the TNI / Police / Retired with a percentage of 0.25%. For the characteristics of the place of residence of the respondents, most of them are found in South Denpasar and the least in East Denpasar with a percentage of 32.5% and 16.75% respectively. The majority of respondents knowledge is known that most people in Denpasar understand that people who are obese will be susceptible to COVID-19 infection with good and sufficient percentages of 44% and 53% respectively.  Based on the results of the research and observations, it can be concluded that the people of Denpasar City have a high level of knowledge about the impact of obesity in relation to COVID-19. Keywords: COVID-19, Level of knowledge, Obesity, Society
Pemanfaatan Ekstrak Buah Merah (Pandanus Conoideus) Dan Ubi Jalar Ungu (Ipomoea Batatas Poiret) Sebagai Pengganti Carbol Fuchsin Dan Methylene Blue Untuk Pewarnaan Bta Akulina Lokbere, Akulina; Iriani, Yulidia; Laksmita W, Ayu Saka
Bali International Scientific Forum Vol. 6 No. 1 (2025): Bali International Scientific Forum
Publisher : Bali International University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34063/bisf.v6i1.492

Abstract

Latar belakang: Pada saat ini, zat warna yang digunakan untuk pewarnaan dibidang mikrobiologi harganya cenderung mahal juga sulit didapatkan. Penyakit TB paru merupakan penyakit infeksi yang disebabkan oleh bakteri Mycobacterium tuberculosis. Deteksi awal penyakit tuberculosis dilakukan dengan pemeriksaan BTA. Tujuan: Untuk mengetahui ekstrak buah merah (Pandanus conoideus) dan ubi jalar ungu (Ipomoea batatas poiret) dapat dijadikan sebagai pengganti Carbol Fuchsin dan Methylene Blue dalam pewarnaan BTA dengan melihat sensitivisitas dan spesifisitas, juga untuk mengetahui konsentrasi optimal yang bisa digunakan untuk pewarnaan BTA bila dibandingkan dengan pewarnaan Ziehl Neelsen. Metode: Rancangan yang digunakan pada penelitian ini adalah Uji Diagnostik Non Priority. Hasil: Pemeriksaan BTA secara mikroskopis pada 38 sampel dengan pewarnaan menggunakan ekstrak buah merah konsentrasi 80% didapatkan hasil true positive 3 orang, false positive 4 orang, true negative 30 orang dan false negative 1 orang dengan sensitivitas 75% dan spesifisitasnya 88%, sedangkan pada konsentrasi 60% didapatkan hasil true positive 1 orang, false positive 8 orang, true negative 25 orang dan false negative 4 orang dengan sensitivitas 11% dan spesifisitasnya 75%, hal tersebut tidak terjadi pada ekstrak ubi ungu. Kesimpulan: Ekstrak buah merah 80% lebih optimal dapat digunakan sebagai pengganti carbol fuchsin bila dibandingkan dengan 60%. Ekstrak ubi jalar ungu baik 60% maupun 80% tidak dapat digunakan sebagai pengganti methylene blue dalam pewarnaan BTA.
Identifikasi Jamur Aspergillus sp. pada Kacang Tanah, Kacang Hijau, dan Kedelai di Pasar Tradisional Desa Sukawati sebagai Upaya Evaluasi Keamanan Pangan Purnami, Sang Ayu Made Ary; Laksmita W, Ayu Saka; Iriani, Yulidia
Bali International Scientific Forum Vol. 6 No. 2 (2025): Bali International Scientific Forum
Publisher : Bali International University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34063/bisf.v6i2.493

Abstract

Latar Belakang: Jamur Aspergillus sp. merupakan salah satu kontaminan utama pada bahan pangan yang dapat menghasilkan aflatoksin berbahaya bagi kesehatan manusia. Tujuan: Penelitian ini bertujuan mengidentifikasi keberadaan jamur Aspergillus sp. pada kacang tanah, kacang hijau, dan kedelai yang dijual di Pasar Tradisional Desa Sukawati serta mengevaluasi implikasinya terhadap keamanan pangan. Metode: Penelitian deskriptif eksploratif dengan desain cross-sectional dilakukan pada 63 sampel (masing-masing 21 sampel per jenis kacang). Identifikasi jamur dilakukan secara makroskopik dan mikroskopik setelah isolasi pada media PDA dan inkubasi 2x24 jam pada suhu kamar. Hasil: Ditemukan kontaminasi Aspergillus sp. pada kacang tanah (88%), kacang hijau (82%), dan kedelai (67%) dengan spesies dominan A. flavus, A. niger, dan A. fumigatus. Kesimpulan: Ketiga jenis kacang di Pasar Sukawati menunjukkan kontaminasi tinggi jamur Aspergillus sp. yang berpotensi menurunkan keamanan pangan. Perlu penerapan penanganan pasca panen dan penyimpanan higienis untuk mencegah kontaminasi lebih lanjut.
Analysis of the Level of Knowledge and Availability of Mask Waste Disposal Facilities in the Sanur Beach Area Apriyanthi, Desak Putu Risky Vidika; Laksmita W, Ayu Saka; Widayanti, Ni Putu
Indonesian Journal of Global Health Research Vol 6 No 2 (2024): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v6i2.2769

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The COVID-19 pandemic has created new problems for the environment as evidenced by the large number of masks, both medical masks (single-use) and non-medical masks, found in public places. The discovery of masks that are discarded in any place, especially in beach tourism areas, is something we usually encounter these days. This has a negative impact on the coastal environment, in the long run, it will be one of the causes of climate change, damage to coastal ecosystems, and ecotoxicity. Sanur Tourism Area is one of the most attractive tourist attractions for tourists both domestic and foreign tourists. The aim of this research is to contribute and provide feedback to the local government especially the Sanitation and Environmental Services Department, to be more concerned about the management of mask waste and the providing of its facilities. The design of this study is descriptive to describe the level of knowledge of Sanur Beach visitors regarding the availability of mask waste disposal facilities at Sanur Beach, it is found that beach visitors have a fairly good level of knowledge (45%) in understanding the impact of mask waste disposal on tourism at Sanur Beach. The majority of respondents stated that the mask waste disposal facilities at Sanur Beach are adequate but the correct procedure for disposing of mask waste has not been found. As many as 68% of respondents stated that there is a closed landfill at Sanur Beach for mask waste disposal. The availability of facilities to manage disposable mask waste is another important factor that influences community action in implementing good waste management. For this reason, it is necessary to coordinate with relevant agencies and Sanur village officials and community cadres to negotiate to provide shared bins at several locations on Sanur Beach specifically to accommodate mask waste only to make it easier for visitors to sort their waste and not mix their mask waste again with other domestic waste.
Antifungal Activity of Chinese Belt Herb (Peperomia Pellucida L. Kunth) Ethanol Extract on the Growth of Candida Albicans Widayanti, Ni Putu; Laksmita W., Ayu Saka; Apriyanthi, Desak Putu Risky Vidika; Widnyani, Ni Made
Indonesian Journal of Global Health Research Vol 6 No 3 (2024): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v6i3.3588

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When the host's phagocytic resistance diminishes, Candida albicans can enter the bloodstream and cause a systemic fungal infection known as candidiasis. One fungus that has become resistant to antifungals is Candida albicans. The herb sirih cina is one way to discover novel antifungals made of natural elements. This study aims to determine the antifungal activity of Chinese betel leaf extract against the growth of the Candida albicans fungus. The sirih cina plant (Peperomia pellucida L. Kunth) was used in this experimental work, which used 70% ethanol as a solvent for the extraction process using maceration. Alkaloids, flavonoids, tannins, saponins, and phenolics are among the secondary metabolites found in the ethanol extract of the Sirih cina plant. Using the well diffusion test method, the antifungal efficiency of the ethanol extract of the Sirih cina plant at concentrations of 25%, 50%, 75%, and 100% was evaluated. Candida albicans growth is inhibited by the ethanol extract of the Sirih Cincina plant, which is categorized as medium category and has the highest inhibition zone diameter at 100% concentration of 8.20 ± 0.14 mm.
Implementation of Green Hospital Concept in Solid Medical Waste Management Apriyanthi, Desak Putu Risky Vidika; Widayanti, Ni Putu; Laksmita, Ayu Saka
Indonesian Journal of Global Health Research Vol 7 No 1 (2025): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v7i1.4384

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Hospitals are one of the institutions that use considerable energy and contribute the largest amount of waste. To reduce the impact, initiatives have emerged in the form of greening hospitals for effective use of resources. Green Hospital is a hospital concept that is designed by empowering the potential of nature as the main resource so that it is friendly to the environment and saves energy expenditure. The existence of Green Hospital is needed to overcome climate change that can cause human health problems and the environment, because hospitals are one of the contributors to pollution. In Indonesia, Green Hospital is still a concept that emphasizes effective and efficient use of water and electrical energy, as well as environmentally sound waste management. The purpose of this study is to analyze solid medical waste management in Saraswati Dental and Oral Hospital Denpasar in realizing the Green Hospital concept. This research is qualitative. Data were collected by in-depth interviews with1 staff at the Environmental Health Installation and 3 staff of cleaning service, observation, and document review. Data were analyzed by data reduction, data presentation, and conclusion. Data validity used source triangulation and method triangulation. Medical waste management in Saraswati Dental & Oral Hospital Denpasar is generally in has been accordance with the Indonesian health regulation No. 1204/2004, but does not yet have a policy or direction to realise Green Hospital. To achieve Green Hospital, hospitals can start by making policies to implement non-medical waste minimization and increasing the number of personnel in the field of medical and non-medical waste management.
Comparison of the Antioxidant Activity of Traditional Herbal Loloh from Bali as an Immunity Booster Widayanti, Ni Putu; Laksmita W., Ayu Saka; Apriyanthi, Desak Putu Risky Vidika; Widnyani, Ni Made
Indonesian Journal of Global Health Research Vol 6 No S6 (2024): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v6iS6.5319

Abstract

Loloh is a local wisdom product of the Balinese people that has been passed down from generation to generation, processed from plant leaves. Consumption of loloh is utilized by the community as a preventive measure to prevent disease and increase body immunity. This has great potential to be applied in facing the growing disease trends in the future, starting from infectious diseases and non-infectious diseases (degenerative). Herbal drinks in general made from ginger, turmeric and temulawak have been widely studied. However, until now, the antioxidant power of loloh from Bali is not yet known. The purpose of this study was to determine the comparison of the antioxidant power of traditional Balinese loloh.This study used a descriptive explorative and experimental design by applying the DPPH (2,2-diphenyl-1-picrylhydrazyl) method to determine the antioxidant power of each loloh sample by looking at its IC50 value. The data collection technique uses purposive sampling. The number of samples studied in this study was ten samples including loloh cemcem, beras kencur, rosella, telang, mengkudu, kelor, kunyit asam, temu kunyit, kunyit asam sirih, and cincau hijau. Data analysis was conducted using regression equations. The results of IC50 measurements showed that the traditional loloh that had very strong antioxidant activity was loloh kelor leaves with an IC50 value of 98 ppm, weak antioxidant activity was possessed by loloh rosella flower tea and cincau leaves with IC50 values of 168 ppm and 198 ppm while very weak antioxidants were possessed by loloh telang flowers, cemcem, beras kencur, mengkudu, kunyit asam, temu kunyit, and kunyit asam sirih with IC50 values > 200 ppm.
The Influence of External Factors on Immunomodulatory Consumption Practices in Health Workers During The Covid-19 Pandemic Widayanti, Ni Putu; Laksmita W., Ayu Saka; Apriyanthi, Desak Putu Risky Vidika
JURNAL INFO KESEHATAN Vol 23 No 1 (2025): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol23.Iss1.1264

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The need for the consumption of immunomodulators is very important to support the body’s immunity of health workers during the Covid-19 pandemic to remain prime in providing services. Because the helath of healthcare workers during the pandemic determines the service that will be provided to patients. This study aims to analyze the influence of external factors on the practice of consuming immunomodulators in health workers at the Denpasar City Health Center. This research is non-experimental research using a cross-sectional method. The population of the research is 550 people and the sample size used in this study amounted to 232 people who have met the inclusion criteria. The results obtained for R square were 0.605 which indicated that the support of the head of the Health Center, family support, co-worker support, and advertising promotion had a large role, namely as much as 60.5% in influencing the practice of consuming immunomodulators in health workers. The R-value obtained in this study was 0.778; F of 86,852; and p of 0.000 (p<0.05) where the hypothesis is accepted, which means that there is a significant influence of external factors on the practice of consuming immunomodulators. There is a significant relationship between the support of the head of the Health Center, family support, co-worker support, and advertising promotion on the practice of consuming immunomodulators. External factors greatly influence healthcare workers' attitudes toward maintaining their health, one of which is their responsiveness in consuming immunomodulators during the pandemic.                    
Pemeriksaan Kadar Gula Darah Sewaktu pada Remaja di SMAN 7 Denpasar Laksmita, Saka; Apriyanthi, Desak Putu Risky Vidika; Widayanti, Ni Putu; Aryasa, Wayan Tanjung; Artini, Ni Putu Rahayu; Iriani, Yulidia
Poltekita: Jurnal Pengabdian Masyarakat Vol. 4 No. 3 (2023): Juli-September
Publisher : Pusat Penelitian & Pengabdian Masyarakat Poltekkes Kemenkes Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33860/pjpm.v4i3.2797

Abstract

People in urban areas tend to have unhealthy lifestyles, especially teenagers. This can trigger various diseases. Health status needs to be considered from an early age, especially paying attention to the intake of sugar in the body. Excess sugar levels in the blood are one of the causes of diabetes mellitus. Measurement of blood sugar (GDS) in adolescents of SMAN 7 Denpasar aims to screen for Diabetes Mellitus (DM) to detect this disease early. The method is done by checking the blood sugar test (GDS), weight, and height. The results of the blood sugar test in this community service activity showed that out of 50 students with a ratio of male and female percentages of 40% and 60% respectively, only 4% were identified with low blood sugar levels while the remaining 96% had normal blood sugar levels. The conclusion of the community service at SMAN 7 Denpasar is that most of the students have normal blood sugar levels.