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All Journal HAYATI Journal of Biosciences Jurnal Fitopatologi Indonesia Agrovigor: Jurnal Agroekoteknologi Agrimeta: Agrimeta: Jurnal Pertanian Berbasis Keseimbangan Ekosistem Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Media Agribisnis (MeA) Jurnal Aplikasi dan Inovasi Iptek (JASINTEK) SEAS (Sustainable Environment Agricultural Science) Agrofarm, Jurnal Agroteknologi
Listihani, Listihani
Faculty Of Agriculture And Business, University Of Mahasaraswati Denpasar, Denpasar, Bali, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Medicine & Pharmacology Other

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Journal : Jurnal Perlindungan Tanaman Indonesia

 1 
Molecular Characterization of Begomovirus on Cucumber in Java Listihani Listihani; Tri Asmira Damayanti; Sri Hendrastuti Hidayat; Suryo Wiyono
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.41402

Abstract

A survey on several cucumber cultivation areas in West Java, Central Java, Yogyakarta, and East Java found many plants showing typical Begomovirus symptoms such as yellow mosaic, cupping, and vein banding. This study was aimed to determine disease frequency, detection and molecular characterization of the causal virus of those symptoms on cucumber in Java. Sampling was conducted by purposive sampling by collecting 50 symptomatic plants from each location in West Java (Indramayu, Subang, and Bogor), Central Java (Brebes and Klaten), Yogyakarta (Kulon Progo), and East Java (Nganjuk, Kediri, and Tulungagung). The detection and disease frequency was determined based on DIBA test using a specific antiserum of Tomato leaf curl New Delhi virus (ToLCNDV) and Squash leaf curl virus (SLCV). The identification of nucleic acid was conducted by PCR using specific primer of ToLCNDV and SLCV, DNA cloning, and sequencing. The results of serological detection showed the disease frequency of ToLCNDV and SLCV ranged from 92.77-100% and 78.33-93.3%, respectively. PCR using specific primer of ToLCNDV successfully amplified the coat protein gene at a size of 600 bp from all samples. Homology nucleotide and amino acid sequences among ToLCNDV Java isolate ranging from 95.6-99.2% and 99.7-100%. ToLCNDV isolates Java had highest nucleotide and amino acid sequences similarity with cucumber isolate from Klaten, Indonesia (AB613825) ranging from 96.1-98.1% and 99.7-100%, and was considered as “Indonesia” strain. SLCV not amplified on all samples by PCR using specific primer, indicating it might not present yet on cucumber in Java.
Biology and the Statistic Demographic of Aphis glycines Matsumura (Hemiptera: Aphididae) on the Soybean with Plant Growth Promoting Rhizobacteria (PGPR) Treatment Hermanu Triwidodo; Anggun Agustini; Listihani Listihani
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 1 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.49846

Abstract

A correction has been published:Erratum to “Biology and the Statistic Demographic of Aphis glycines Matsumura (Hemiptera: Aphididae) on the Soybean with Plant Growth Promoting Rhizobacteria (PGPR) Treatment” [Jurnal Perlindungan Tanaman Indonesia, 24(1), 54 ̶ 60]Plant Growth Promoting Rhizobacteria (PGPR) applied to different plants may suppress pests population developments. This research was to study the capability of a commercial PGPR product contained Bacillus polymyxa and Pseudomonas fluorescens in suppressing population developments of Aphis glycines Matsumura (Hemiptera: Aphididae). The biology and demographic statistics of A. glycines reared on soybean with and without the PGPR applications were compared. The PGPR suspensions of 5 g formulation per liter water were used to soak soybean seed for 15 minutes and to water soybean plant 2 weeks after transplanting. Cohorts of 65 first instar A. glycines of each treatment were observed daily and individual mortality, molting, and fecundity were recorded until the last individual dead. Second instar stadium of A. glycines reared on treated plant lasted longer than those reared on untreated plant, i.e. 1.4 and 1.1 days, respectively. These resulted on a longer life cycle for A. glycines reared on treated plant than on untreated plant, i.e. 4.9 and 4.5 days, respectively. In turn, it caused the A. glycines population to experienceslower growth on treated plants than on untreated plants.  The values of A. glycines GRR, Ro, rm, T and DT on treated plants were 71.834, 57.780, 0.557, 7.287 and 1.245, consecutively; whilst that of untreated plants were 104.861, 63.326, 0.586, 7.084 and 1.184, respectively.
Molecular Identification of Sweet potato virus C on Sweetpotato in Bali, Indonesia Listihani Listihani; Dewa Gede Wiryangga Selangga
Jurnal Perlindungan Tanaman Indonesia Vol 25, No 1 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.64545

Abstract

A survey was conducted in several sweet potato cultivations in Bali Province. Survey found that many plants exhibited potyvirus symptom, such as chlorosis blotches. This study was to determine disease incidence, detection and identification of the virus causing these symptoms on sweet potato plants in Bali. Samples were collected by purposive sampling of 10 plants from each location in Bali (Denpasar, Gianyar, Badung, Buleleng, Tabanan, Klungkung, Karangasem, Jembrana, Bangli). Disease insidence was observed based on viral symptoms in the field. Identification of nucleic acids was done using Potyvirus universal primer and DNA sequencing. Disease incidence in Bangli, Buleleng, and Denpasar Regencies was > 50%. RT-PCR and CiFor/CiRev Potyvirus universal primers successfully amplified ± 700 bp of CI genes from all samples from Bangli, while samples from 8 other districts were not amplified using the same primers. The SPVC isolate of sweet potato showed nucleotide and amino acid homology similarities with the sweet potato isolate from East Timor (MF572066), 96.8% and 97.4%, respectively and these were referred to the "Asian" strain. This indicates that SPVC has spread in East Java and Bali.
The Existence of Papaya ringspot virus-Papaya Strain on Cucumber in Gianyar, Bali Dewa Gede Wiryangga Selangga; I Ketut Widnyana; Listihani Listihani
Jurnal Perlindungan Tanaman Indonesia Vol 25, No 1 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.64703

Abstract

Yellow mosaic symptoms were identified from cucumber production systems in Gianyar and were similar to symptoms of PRSV infection. Further research was conducted to determine diseases incidence and molecular characteristic of PRSV. Ninety leaf samples were collected from Gianyar by purposive sampling and disease incidence calculations were based on symptoms in the field. Detection and identification were done using a RT-PCR with specific primers of CP PRSV-P, CP PRSV-W and DNA sequencing. Disease incidences in the fields ranged between 5.81–66.87%. Specific DNA band 470 bp was successfully amplified from several cucumber leaf samples collected from Ubud, Payangan, Tegallalang, Sukawati, Gianyar, and Blahbatuh; but no DNA were amplified from all samples when using CP PRSV-W specific primer. Nucleotide and amino acid analysis showed nucleotides homology to isolates from Ubud, Payangan, Tegallalang, Sukawati, Gianyar, and Blahbatuh, i.e. 98.9–99.5% and 99.1–100%, respectively. Results indicated that genetic variation of PRSV-P from Gianyar was low. Furthermore, the nucleotides homology of PRSV-P isolates from Ubud, Payangan, Tegallalang, Sukawati, Gianyar, and Blahbatuh were with PRSV-P isolates which infected cucumbers from Nganjuk (LC311783) and Brebes (LC311784), while from native papaya collected in Bali Bali (LC223115) were 97.2–98.4% and 98.6–100%, respectively. Phylogenetic analysis confirmed that PRSV-P isolates from Indonesia were in the same cluster with Thailand isolates. The results showed that sources of PRSV-P inoculums spreading into new areas.
Co-Authors Anggun Agustini Arisandhi, I Made Dimas Bagus Putu Udiyana, Bagus Putu Cokorda Javandira Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dinarkaya, Shah Mahapati Dwi Susanti, Ida Ayu Made Gusti Ngurah Alit Susanta Wirya Hartha , I Komang Gede Suweca Hermanu Triwidodo I Gusti Ayu Diah Yuniti I Ketut Widnyana I Made Budiasa I MADE SUKERTA I Made Suryana I Putu Sudiarta I Putu Sujana Ida Ayu Made Dwi Susanti Ignasius Sandriawan KETUT AYU YULIADHI Ketut Ayu Yuliadhi Mimi Sutrawati Mimi Sutrawati Modesta Yuliana Hartika Ni Putu Pandawani Oktavianus Bulu Daka Putu Eka Pasmidi Ariati Ramdhoani Selangga , Dewa Gede Wiryangga Selangga, Dewa Gede Wiryangga SRI HENDRASTUTI HIDAYAT Suputra , I Putu Wirya Suryo Wiyono TRI ASMIRA DAMAYANTI TRISNA AGUNG PHABIOLA TRISNA AGUNG PHABIOLA Widiantara, I Gusti Lanang Yuliadhi , Ketut Ayu