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All Journal HAYATI Journal of Biosciences Jurnal Fitopatologi Indonesia Agrovigor: Jurnal Agroekoteknologi Agrimeta: Agrimeta: Jurnal Pertanian Berbasis Keseimbangan Ekosistem Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Media Agribisnis (MeA) Jurnal Aplikasi dan Inovasi Iptek (JASINTEK) SEAS (Sustainable Environment Agricultural Science) Agrofarm, Jurnal Agroteknologi
Listihani, Listihani
Faculty Of Agriculture And Business, University Of Mahasaraswati Denpasar, Denpasar, Bali, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Medicine & Pharmacology Other

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Squash Leaf Curl Virus: Species of Begomovirus as the Cause of Butternut Squash Yield Losses in Indonesia Dewa Gede Wiryangga Selangga; Listihani Listihani
HAYATI Journal of Biosciences Vol. 29 No. 6 (2022): November 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.6.806-813

Abstract

Curling symptom was found in pumpkin plants in Bali, and the PCR detection result using Begomovirus universal primers indicated Begomovirus infection. Further research was conducted to determine the distribution, molecular character of SLCV, and yield loss of the pumpkins. Sampling was carried out at pumpkin plantations in 9 districts in Bali. Detection and identification were carried out using PCR method, followed by cloning and DNA sequencing. DNA band with the measurement of 900 bp was successfully amplified from several pumpkin samples from Denpasar, Gianyar, and Buleleng. Homology analysis of nucleotide sequences using the database in GenBank of SLCV of Balinese isolates showed the highest homology and kinship of 97.3-98.4% and 98.4-99.3% respectively with East Timor isolates from pumpkin plants. The phylogeny analysis showed that SLCV Indonesian isolates were in the same group as Asian isolates. The result of this study is the first report on infection and molecular characterization of SLCV in pumpkin plants in Indonesia. The yield loss caused by curling disease on individual pumpkin plants was 56.3%, and the disease caused a decrease in the quality of harvest fruits. Yield loss estimation caused by the disease in fields with different levels of disease intensity ranged from 10.02 to 25.83%. It was equal to yield loss ranging from IDR 878,400.00 to IDR 10,826,400.00 depending on the disease intensity. It is found that the correlation between curling disease severity and yield loss was high.
Distribution and molecular characterization of Squash mosaic virus on cucumber in Gianyar, Bali Listihani Listihani; Ni Putu Pandawani; Tri Asmira Damayanti; Mimi Sutrawati; Dewa Gede Wiryangga Selangga; Ketut Ayu Yuliadhi; Trisna Agung Phabiola; Gusti Ngurah Alit Susanta Wirya
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 22 No. 1 (2022): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12248-54

Abstract

Squash mosaic virus (SqMV) has been reported to infect cucumber plants in Java and cause a decrease in fruit quality and quantity. Until now, there is no information regarding Sqmv infection in Cucurbitaceae in Bali. In a preliminary research conducted during 2020, we found mosaic symptoms in Gianyar, Bali. SqMV was inferred to be the possible cause of the virus-disease-like symptoms. The study aimed to determine the precence of SqMV in Gianyar, Bali and to obtain information related to its molecular character. This research method includes surveys, field observations, virus identification by RT-PCR, and DNA analysis. Severe disease incidence caused by SqMV was observed on cucumber cultivation in Gianyar that was in the range of 5.81–66.87%. PCR using specific primer for coat protein (CP) gene of SqMV was successfully amplified the DNA fragments of ± 582 bp on samples from Payangan, Tegallalang, Ubud, Sukawati, Blahbatuh, and Gianyar districts, except for samples from Tampaksiring. This indicates that the presence of SqMV is widespread in Java and Gianyar.The SqMV isolate from Bali had the highest nucleotide homology at 91.9–93.4% and amino acids 94.0–94.5 and was closely related to the Brazil isolate (KT923125) and had lower isolate homology than other countries (China, Trinidad, Spain, Japan, Arizona, Cekoslowakia, and Australia). It proved that those mosaic symptoms on cucumber is associated with SqMV infection. SqMV is classified as a quarantine organism of category A2, so it is necessary to prevent its spread to other areas.
RESPON PERTUMBUHAN DAN HASIL TANAMAN KANGKUNG DARAT (Ipomea reptans poir) TERHADAP PEMBERIAN PUPUK KANDANG DAN BIOURINE SAPI Modesta Yuliana Hartika; I Made Sukerta; Listihani; Bagus Putu Udiyana
Agrofarm: Jurnal Agroteknologi Vol. 2 No. 2 (2023): Agrofarm: Jurnal Agroteknologi
Publisher : Faculty of Agriculture and Business, Universitas Mahasaraswati Denpasar , Kec. Denpasar Utara, Kota Denpasar, Bali 80233

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Abstract

Kale plans is one type of horticultural plant that is easy to cultivz kale plants can grow optimally if they are planted on land that has high enough macro and micro nutrients and loose soil conditions, one of the macro nutrients needed by this vegetable is nitrogen nutrients, because nitrogen is a basic nutrient forming proteins, nucleic acids, and chlorophyll that are useful in the process of photosynthesis. This study aims to determine the "Response of Growth and Yield of kale plans (Ipomea reptans poir). Due to Dosage of Manure and Concentration of Cattle Biourine. The method used is a Randomized Block Design (RAK) with factorial treatment with 2 treatment factors. Factor 1: Dosage of cow manure with 5 levels, namely K0 (without cow manure), K1 (cow manure 30 gr/10 kg of soil), K2 (cow manure 60 gr/10 kg of soil), K3 (cow manure 90 g / 10 kg of soil), and K4 (cattle manure 120 g / 10 kg of soil). Factor 2: concentration of bovine biorin with 5 levels, namely B0 (without beef biorin), B1 (bovine biorin 80 ml/1 liter of water), B2 (bovine biorin 160 ml/1 liter of water), B3 (bovine biorin 240 ml/1 liter water), and B4 (beef biorin 320 ml/1 liter of water). The results showed that treatment (K4B4) with a dose of cow manure 120 g/10 kg of soil with a concentration of biourin 320 ml/1 liter of water gave the best growth compared to other treatments for all observation parameters such as plant height (cm), number of leaves (strands). , leaf area (cm²), total fresh weight (g), total oven dry weight (g), fresh weight without roots (g), and oven dry weight without roots (g). and the lowest was in the K0B0 treatment without a dose of manure and cow biourin concentration.
PENGARUH PEMBERIAN PUPUK ORGANIK KOTORAN SAPI TERHADAP PERTUMBUHAN DAN HASIL TANAMAN BAWANG MERAH (Allium ascalonicum L) I Made Suryana; I Gusti Ayu Diah Yuniti; Listihani; Ramdhoani; Oktavianus Bulu Daka
Agrofarm: Jurnal Agroteknologi Vol. 3 No. 1 (2024): AGROFARM (JURNAL AGROTEKNOLOGI)
Publisher : Faculty of Agriculture and Business, Universitas Mahasaraswati Denpasar , Kec. Denpasar Utara, Kota Denpasar, Bali 80233

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Abstract

The title of this research is “the effect of organic cow dung fertilizer on the growth and yield of shallot (Allium ascanolicum L). The purpose of this study was to determine the effect of the use of organic cow dung fertilizer and the best dose in the use of organic cow dung fertilizer on onion planting media. This research was conducted in Kesiman Village, East Denpasar District, Denpasar City. This research was conducted over a period of three months, starting from January 22 to March 25, 2021. To test the effect of giving organic cow dung fertilizer using the Randomized Block Design (RAK) method with six treatments, namely treatment (P0) = without giving organic manure fertilizer cattle, (P1) = 5 tons/ha organic cow dung, (P2) = 10 tons/ha organic cow dung, (P3) = 15 tons/ha organic cow dung, (P4) = 20 tons/ha fertilizer organic cow dung, (P5) = 25 tons/ha organic cow dung. Each treatment was repeated four times so that the total number of treatments became 24. The results of statistical analysis showed that the application of organic cow dung fertilizer on shallot plants had a significant effect on all parameters observed, except for the parameters of plant height and leaf fresh weight. The results showed that the application of organic cow manure 20 tons/ha (P4) was able to give the best results on the fresh weight of tubers, namely 66.68 g. And the oven-dry weight of tubers was 39.37 g.
Pengaruh Pemberian Biourine Sapi Pada Sistem Hidroponik Terhadap Pertumbuhan dan Hasil Tanaman Kangkung Darat I Putu Sujana; Cokorda Javandira; Listihani; Ignasius Sandriawan
AGRIMETA : Jurnal Pertanian Berbasis Keseimbangan Ekosistem Vol. 13 No. 26 (2023): Agrimeta: Jurnal Pertanian Berbasis Keseimbangan Ekosistem
Publisher : Fakultas Pertanian dan Bisnis Universitas Mahasaraswati Denpasar

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Abstract

This study aims to determine the effect of giving different cow biourine in the hydroponic system on the growth and yield of land kangkung plants to get the right cow biourine administration in the hydroponic system so that the best growth and yield of kale plants is obtained. The design used in this study was a randomized block design (RAK). With 6 treatments of giving cow biourine, K1: cow biourine giving 100 ml/1 liter of water, K2: 200 ml/1 liter, K3: 300 ml/1 liter, K4: 400 ml/1 liter, K5: 500 ml/1 liter and K6: 600 ml/1 liter of water. With 4 replications and 24 trials. The conclusion of this study was that the highest yield of land kangkung in a hydroponic system was obtained by giving biourine 300 ml/1 liter of water such as the highest plant height (K3 = 45.80 cm), the highest number of leaves (K3 = 15.00 pieces), leaf area (K3=102.25 cm), total wet weight of plants (K3=40.03 g) and oven-dry weight of plants (K3=2.19 g). giving biorine 300 ml/1 liter of water is the right way to get the best yield of land kale in a hydroponic system compared to giving 100 ml/1 liter of water; administration of 200 ml/1 liter of water; administration of 400 ml/1 liter of water; administration of 500 ml/1 liter of water; administration of 600 ml / 1 liter of water.
Swasembada Pakan Ikan Melalui Pelatihan Pembuatan Pelet Ikan Dan Pakan Tepung Berbahan Dasar Magot Pada Pokdakan Ulam Merta Asih Susanti, Ida Ayu Made Dwi; Budiasa, Made; Listihani, Listihani
Jurnal Aplikasi dan Inovasi Iptek Vol 5 No 1 (2023): Jurnal Aplikasi dan Inovasi Iptek No. 5 Vol. 1 Oktober, 2023
Publisher : Denpasar Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52232/jasintek.v5i1.118

Abstract

Kegiatan pelatihan pembuatan pakan ikan berbahan dasar magot bertujuan untuk memandirikan pokdakan Ulam Merta Asih dalam mengurangi pembelian pakan. Karena dalam pembudidayaan ikan sangat bergantung pada biaya pakan. Metode pengabdian dialkuakn melalui penyuluhan dan pelatihan di Pokdakan Ulam Merta Asih, Desa Jehem, Kabupaten Bangli dengan diikuti oleh 13 peserta. Hasil evaluasi melalui kuisioner pre test dan pots test dapat kita lihat bawah ketertarikan peserta terhadap pengetahuan dan pelaksanaan budidaya magot serta pembuatan pakan ikan meningkat setelah dilakukannya penyuluhan dan pelatihan. Dari yang sebelumnya kurang tertarik sebesar 62% menjadi 53,8% sangat tertarik pada budidaya magot. Pada pembuatan pakan ikan ketertarikan meningkat dari 69,2% cukup tertarik menjadi 38,5% tertarik dan 61,50% sangat tertarik
Strategi Pengembangan Wisata Kuliner Olahan Beras di Desa Wisata Sidan Widiantara, I Gusti Lanang; Dwi Susanti, Ida Ayu Made; Budiasa, I Made; Listihani, Listihani
Jurnal MeA (Media Agribisnis) Vol 9, No 2 (2024): Oktober
Publisher : Universitas Batangahari Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33087/mea.v9i2.256

Abstract

Organic products are products (plant/livestock products that are produced through practices that are ecologically, socio-economically sustainable, and of good quality (nutritional value and safety against toxins are guaranteed). Organic rice for milk in the Tourism Village has an organic certificate. So, To increase opportunities to introduce organic rice products in the Sidan Tourism Village, innovation is needed in the culinary sector, especially culinary tourism. The aim of this research is to design a strategy based on program objectives through the ISM method in the Sidan Tourism Village. Through ISM analysis, a tourism development strategy can be obtained. culinary processed rice in Sidan Tourism Village.
Symptoms variation and molecular characterization of Strawberry vein banding virus in Bali, Indonesia Phabiola, Trisna Agung; Selangga, Dewa Gede Wiryangga; Wirya, Gusti Ngurah Alit Susanta; Sudiarta, I Putu; Dinarkaya, Shah Mahapati; Listihani, Listihani
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 1 (2025): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12544-53

Abstract

Vein banding disease has affected young strawberry leaves in Bali over the past five years. Caused by Strawberry vein banding virus (SVBV), the disease is primarily associated with fruit size reduction, though its exact impact remains unclear. This study aims to assess symptom variation, disease severity, and the molecular characteristics of SVBV in Bali strawberry plants. Field observations and molecular identification were conducted using PCR with SVBV-specific primers targeting the CP gene. Samples were collected from ten locations in Bali, including Pancasari, Candikuning, Wanagiri, Gobleg, and Kembang Merta. SVBV DNA from Candikuning, Pancasari, and Kembang Merta was successfully amplified, confirming SVBV infection as the cause of vein banding symptoms. SVBV induces vein banding with upward and downward leaf curling. Disease incidence was highest in Pancasari 1 and 3 (80%) and lowest in Wanagiri 1 and Gobleg (20–28%). Disease severity ranged from 13% to 83%, with the highest recorded in Pancasari, Buleleng, and the lowest in Kembang Merta, Tabanan. Molecular analysis revealed that the SVBV isolate from Bali shares 99.3–100% sequence homology with Chilean isolates. Phylogenetic analysis showed clustering with SVBV isolates from the United States, Brazil, and Chile. This study provides the first molecular characterization of SVBV in Bali, contributing to a better understanding of its epidemiology and potential impact on strawberry production.
The Effect of media types on the growth of insect pathogenic fungi (Aschersonia placenta) Sudiarta, I Putu; Hartha , I Komang Gede Suweca; Yuliadhi , Ketut Ayu; Wirya, Gusti Ngurah Alit Susanta; Selangga, Dewa Gede Wiryangga; Suputra , I Putu Wirya; Listihani, Listihani
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 1 (2025): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12554-62

Abstract

Bali has favorable conditions for the production and expansion of citrus plantations. However, citrus cultivation in Bali often faces challenges, including reduced yield caused by pest infestations and pathogenic diseases. One significant pest affecting citrus plants is the whitefly. A potential method for controlling whiteflies is the use of natural enemies. The entomopathogenic fungus Aschersonia placenta is one of natural enemy that can effectively manage whitefly infestations. However, the succesful utilization of A. placenta requires a specialized approach, particularly in selecting an appropriate growth medium. This study aimed to investigate the impact of different growth media on the development of the entomopathogenic fungus A. placenta. The research began with field sampling, followed by the isolation and morphological identification of A. placenta to obtain a pure culture. Subsequently, media tests were conducted to evaluate fungal growth. Nine treatments were implemented to examine the impact of different media: (1) Water Agar (WA), (2) Potato Dextrose Agar (PDA), (3) Potato Sucrose Agar (PSA), (4) Water Agar + Weaver Ant Eggs Flour (WA-WAEF), (5) Potato Dextrose Agar + Weaver Ant Eggs Flour (PDA -WAEF), (6) Potato Sucrose Agar + Weaver Ant Eggs Flour (PSA-WAEF), (7) Water Agar + Cricket Flour (WA-CF), (8) Potato Dextrose Agar + Cricket Flour (PDA-CF), and (9) Potato Sucrose Agar + Cricket Flour (PSA-CF). The results demonstrated that incorporating cricket insect flour (Gryllidae: Orthoptera) or weaver ant egg flour (Oecophylla smaragdina) into potato sucrose media provided the most optimal growth environment for A. placenta.
Molecular characterization of Rice ragged stunt virus and Rice grassy stunt virus on Rice in Gianyar, Bali, Indonesia Listihani, Listihani; Selangga, Dewa Gede Wiryangga; Yuliadhi, Ketut Ayu; Yuniti, I Gusti Ayu Diah; Ariati, Putu Eka Pasmidi
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 24 No. 1 (2024): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12448-57

Abstract

Rice stunt disease is one of the causes of rice harvest failure. It is caused by the infection of Rice ragged stunt virus (RRSV) and Rice grassy stunt virus (RGSV) infection. Information about disease severity and the molecular characteristics of stunt viruses in Indonesia is still limited. Thus, this research aimed to determine the disease severity and the genetic diversity of rice stunt viruses in Gianyar, Bali. The research method consisted of observation of incidence and disease severity in the field and virus detection by reverse transcription-polymerase chain reaction (RT-PCR) using primers specific for RRSV and RGSV. The observation of the disease incidence and severity were performed in seven districts in Gianyar Regency, Bali, namely Blahbatuh, Gianyar, Payangan, Sukawati, Tampaksiring, Tegallalang, and Ubud. Stunt disease was found in all observation sites. High stunt disease incidence (> 44%) was found in three districts: Ubud, Tampaksiring, and Payangan, while the low disease incidence rate of <10% was found in Blahbatuh and Gianyar Districts. The highest stunt disease severity occurred in Tampaksiring District (60.82%), while the lowest severity occurred in Gianyar District (18.84%). The IR-64 and Ciherang cultivars are vulnerable to rice stunt disease infection. The highest homology of RRSV and RRGV nucleotides was found with Vietnam isolates being >98% and >97%, respectively. The phylogenetic analysis showed that Indonesian isolates of RRSV and RGSV were clustered in the same group as Vietnam isolates.
Co-Authors Anggun Agustini Bagus Putu Udiyana, Bagus Putu Cokorda Javandira Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dinarkaya, Shah Mahapati Dwi Susanti, Ida Ayu Made Gusti Ngurah Alit Susanta Wirya Hartha , I Komang Gede Suweca Hermanu Triwidodo I Gusti Ayu Diah Yuniti I Ketut Widnyana I Made Budiasa I MADE SUKERTA I Made Suryana I Putu Sudiarta I Putu Sujana Ida Ayu Made Dwi Susanti Ignasius Sandriawan KETUT AYU YULIADHI Ketut Ayu Yuliadhi Mimi Sutrawati Mimi Sutrawati Modesta Yuliana Hartika Ni Putu Pandawani Oktavianus Bulu Daka Putu Eka Pasmidi Ariati Ramdhoani Selangga , Dewa Gede Wiryangga Selangga, Dewa Gede Wiryangga SRI HENDRASTUTI HIDAYAT Suputra , I Putu Wirya Suryo Wiyono TRI ASMIRA DAMAYANTI TRISNA AGUNG PHABIOLA TRISNA AGUNG PHABIOLA Widiantara, I Gusti Lanang Yuliadhi , Ketut Ayu