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Journal : Medical Laboratory Technology Journal

Quality of Genom in Type II Diabetes Mellitus Patients in Viewed of Temperature, Storage Duration, Number of Leukocyte Puspitasari Puspitasari; Evi Rinata; Rohman Dijaya; Siska Aprilia; Dina Trikumalasari; Livia Nur Azzah; Qilmia Fanani; Miftahul Mushlih; Andika Aliviameita; Dian Delta
Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (602.348 KB) | DOI: 10.31964/mltj.v5i2.229

Abstract

Type II Diabetes Mellitus is a disorder caused by genetic and environmental factors. Molecular analysis of T2DM abnormalities has been carried out. But the analysis in sample preparation especially the stages of DNA isolation has not been done much. The aims of this study are to investigate the effect of temperature, storage duration and level of white blood cell (WBC) with Genome Quality in T2DM. The treatment (n:10) which were divided into several tubes and then stored at 4 °Ϲ, 25 °Ϲ and 32 °Ϲ for 21 days. To determining storage duration effect, we use periodically isolation of DNA (3, 15 and 30 days) after sampling. The effect of WBC with DNA quality was carried out using 17 samples. DNA isolation was done by the DNA Isolation Kit manual without modification and then tested qualitatively and quantitatively. Based on this research, it can be concluded there is a correlation between the numbers of WBC with DNA quality. The higher the number of WBC, the higher DNA concentration (r: 0.818. p value: 0.000). The concentration of DNA at a temperature of 4°Ϲ (135.1 ± 165.2 ng / µl) was higher compared with the temperature treatment 25 ° Ϲ (29.7 ± 36.5 ng / µl) and 32 ° Ϲ (22.14 ± 7.13 ng / µl) (p
Co-Authors Agmar, Alfisyar Agus Muji Santoso Agus Salim Agus Syahrani Agus Syahrani Agustiningrum, Rita Aini, Latifatul Alia Bihrajihant Raya Alia Bihrajihant Raya Anang Wahid M.Diah Andayani, Gita Ayu Andayani, Gita Ayu Andika Aliviameita Anggraeni Dyah S Anggraeni Dyah Sulistiowati Anggraini, Sekarlita Normaulida Arifin Dwi Saputro Azhari, Tontowi Azizah, Nurul Bachtiar, Willy Baso, Siti Nur Azizah Sucitra Cholifah , Siti Dewi Izzatus Tsamroh Dewi Komala Sari Dian Delta Dina Trikumalasari Dira Nadila Effy Wardati Maryam Finda Meyditia Finda Meyditia Fitriani, Dwi Ayu Hamdi, Hafmi Putri Syahilda Hendy Setiawan Hendy Setiawan Henny hidayanti Herlina, Lien Hesti Widowati Iflahah, Dini Ika Ratna Indra Astutik Indahyanti , Uce Isna Fitria Agustina Istiqomah , Dani Kartikasari, Dwi Ajeng Khasanah, Nur Riska Alfiyah Livia Nur Azzah M. Suryawinata M. Suryawinata Miftahul Mushlih Muntadir, Lila Nanang Wahyudin Nanang Wahyudin Normaulida Anggraini, sekarlita NURUL AZIZAH Okky Elvira Novianti p Puspitasari, p Paramitha Amelia Kusumawardani Pardede, Akim Manaor Hara Prigi Dewi Gita Aristanti Pungzup, Saowaros Puspitasari Puspitasari Puspitasari Puspitasari Puspitasari, Dini Ayu Putri Anjar Sari Putri Anjar Sari Putri, Arizki Amalia Qilmia Fanani Rafhani Rosyidah Rafhani Rosyidah rima siburian Rohman Dijaya Ronny Lesmana Rosyidah, Rafhani Rusdyati, Tutik Saidah, Sukma Nur Salim, Siti Zaenab Sari, Dwi Siska Mayang Sari, Putri Anjar Setyawardana, Eka Siska Aprilia Siti Zaenab Salim Soekarno, Krisjayanti Sri Mukhodim Faridah Hanum Tontowi Ashari Tsabita, Nabila Fatya Tutik Rusdyati Widowati, Hesti Widowati, Hesty Widya Nurfadillah Widyarini Susilo Putri Willy Bachtiar Yanik Purwanti Yanik Purwanti Yanuar Dwi Prastyo Yasmin, Rizka Afifah Yonika