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Elfita
Jurusan Kimia Fakultas Matematika Ilmu Pengetahuan Alam, Universitas Sriwijaya, Inderalaya, Sumatera Selatan
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Journal : Science and Technology Indonesia

 1 
Antioxidant and Antibacterial Activity of Endophytic Fungi Isolated from Fruit of Sungkai (Peronema canescens) Oktiansyah, Rian; Widjajanti, Hary; Setiawan, Arum; Elfita
Science and Technology Indonesia Vol. 9 No. 1 (2024): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2024.9.1.17-27

Abstract

Peronema canescens, often known as sungkai, is widely used and can be found all around Indonesia. The public believes that the leaves may reduce fever and strengthen the immune system. However, the effectiveness of sungkai fruit has not been thoroughly investigated. In this research, we looked at endophytic fungus extracts from sungkai fruit’s that have antioxidant and antibacterial properties. The study’s results will serve as the foundation for further investigation into the development of potential natural chemicals with antioxidant and antibacterial properties. Morphologically, the endophytic fungi isolated from sungkai fruit were identified. The antioxidant and antibacterial properties of endophytic fungal extracts were studied using the DPPH technique and the paper disk diffusion method. By employing molecular identification and column chromatography to separate the active compounds, the most likely endophytic fungal isolates were found based on the results of the bioactivity tests. Using 1D NMR spectroscopic methods, the chemical’s structure was determined, and the results were compared to NMR data for the same compound published in the literature. Fruit of sungkai had 8 strains of endophytic fungus (RBH1-RBH8). Strong antibacterial and very strong antioxidant activity were shown by the RBH5 isolate (IC50 < 20 μg/mL). Pythium periplocum was determined to be the RBH5 isolate based on molecular testing. Pure chemical compound extracted from RBH5 isolates shown highly potent and potent antibacterial and antioxidant effects. The chemical compound was identified by spectroscopy as 3-hydroxy-4(hydroxy(4-hydroxyphenyl)methyl)-γ-butyrolactone. The results of this study serve as the foundation for developing compounds as pharmaceutical raw materials via further research phases.
Combination Effect of Extracts and Pure Compounds of Endophytic Fungi Isolated from Sungkai (Peronema canescens) Leaves on Antioxidant Activity Elfita; Oktiansyah, Rian; Mardiyanto; Setiawan, Arum; Widjajanti, Hary
Science and Technology Indonesia Vol. 9 No. 1 (2024): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2024.9.1.69-76

Abstract

Endophytic fungi extracts have various biological and pharmacological activities as natural antioxidants which have the potential to be developed in the pharmaceutical. Drugs made from natural substances frequently work best when they are present in large quantities. This may result in unfavorable side effects from using more of one substance at a time. This limitation can be overcome by using a combination of the extracts/pure compounds that allow synergistic interactions with strong antioxidant properties at fairly low concentrations. The purpose of this research was to determine the combined effect of endophytic fungi extracts and its pure compounds on their antioxidant activity. This research was initiated by rejuvenating eight selected endophytic fungal isolates from Sungkai leaves and morphological characterization was carried out. Each fungus was cultivated in PDB medium for 4 weeks under statistical conditions. Ethyl acetate was used to extract the endophytic fungi’s liquid culture, which was then evaporated. Each endophytic fungal extract (PD1-PD8) and their five pure compounds were tested for antioxidant activity by the DPPH method. Then a combination of two and three extracts was carried out with a ratio of 1:1. The results show that the interaction of the mixture of endophytic fungi extracts and their pure compounds can be classified as synergistic (combined effect of the extracts > individual effects), additive (combined effect of the extracts = individual effect), and nothing is antagonistic (combined effect of the extracts < individual effects). The best combination in this study was PD4+PD5+PD6 (1:1:1) which could be used as a formula for further research into in vivo immunostimulant tests. Another conclusion from this research is that the pure compounds contained in the extracts have lower antioxidant activity than the extracts and do not have a synergistic effect on the combination.
Bioactivity of Endophytic Fungi Isolates from Syzygium malaccense Twigs Hapida, Yustina; Widjajanti, Hary; Salni; Habisukan, Ummi Hiras; Alfarado, Daniel; Elfita
Science and Technology Indonesia Vol. 9 No. 2 (2024): April
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2024.9.2.448-456

Abstract

Searching for medicinal raw materials derived from the isolation of secondary metabolite compounds is still being carried out to cure a range of illnesses, including infections and degenerative diseases caused by bacteria and free radicals. This research used endophytic fungi extract from the twig of Syzygium malaccense. The diffusion method was used for antibacterial activity and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was used for antioxidant activity. Chemical compound was identified by using NMR spectroscopic method. This research found 5 isolate of endophytic fungi from twig of S. malaccense (YR1 – YR5). Strong antibacterial and antioxidant activity were showed by YR5. Based on molecular identification, YR5 was Fusarium verticillioides. Pure compound prodeced by endophytic fungi F. verticilloides was naphthalene (3-methoxy-7 methylnaphthalene-1,6-diol). This finding can be used as a basis for drug development.
Biodiversity and Analysis of Antioxidant and Antibacterial Activity of Endophytic Fungi Extracts Isolated from Mangrove Avicennia marina Noviyanto; Widjajanti, Hary; Elfita
Science and Technology Indonesia Vol. 10 No. 1 (2025): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.1.139-151

Abstract

Avicennia marina, a mangrove species commonly found along coastal areas, plays both ecological and pharmacological roles, with its plant parts exhibiting antioxidant and antibacterial activities. This study aimed to investigate the diversity of endophytic fungi from various organs of A. marina collected from mangrove ecosystems and to explore and analyze their antioxidant and antibacterial activities. Endophytic fungi were isolated from the roots, stems, and fruits of A. marina using PDA medium and were morphologically identified. Each fungal isolate was cultivated in PDB medium for 4 weeks under static conditions, followed by extraction to obtain concentrated extracts. Antioxidant and antibacterial activities were assessed using the DPPH method and disk diffusion assay. A total of 23 fungal isolates were obtained from the roots, stems, and fruits of A. marina. The identification results showed that the root isolates had the highest genus diversity, followed by the stem and fruit isolates. The highest distribution of antioxidant and antibacterial activities was observed in the endophytic fungal extracts from fruits, followed by those from roots and stems. Notably, the majority of the 23 endophytic fungal extracts exhibited strong antioxidant and antibacterial activities. Isolates AMF3 and AMF6showed the most potent antioxidant activity, classified as very strong, with IC50 values below 20 ug/mL. Morphological identification revealed AMF3 as Neopestalotiopsis sp. and AMF6 as Aspergillus niger. This study highlights the potential of Neopestalotiopsis sp. and Aspergillus niger endophytic fungi from A. marina fruits as sources of natural antioxidant and antibacterial compounds, offering valuable insights for biotechnological applications of mangrove-associated endophytes.
Co-Authors Achmad Ilham Afnidar Agusreni Aldes Lesbani Aldes Lesbani Alfarado, Daniel Amri Arrwais, Rhani Arum Setiawan Arum Setiawan Chandra Irsan Dachriyanus Eliza Eliza Erise Anggraini, Erise Feriska Feriska Ferlinahayati Ferlinahayati Fitrya . Gusti Pratiwi Hajnurisa Aini Hamzah Hasyim Harman Hamidson Hary Widjajanti Hary Widjajanti Heni Yohandini Hong Lau, Wei Hotdelina S Hotdelina S Husein Hernadi Bahti Kurratul Aini Lenny Anwar Mardiyanto Marieska Verawaty Maryadi Maryadi Masyita Masyita Miksusanti Miksusanti Milanti oktaruliza Milinia Puspita Sari, Jelly Mokhamad Yusup Nur Khakim Muharni Muharni Muharni Muharni Munawar Munawar Nelly Erlina Nova Yuliasari Noviyanto Nurlisa Hidayati Oktiansyah, Rian Rahmadania Rahmadania Risfidian Mohadi Risfidian Mohadi Risfidian Mohadi Salni Setiawati Yusuf SITI HERLINDA Sri Hidayati Supriyatna Supriyatna Suwandi Suwandi Ummi Hiras Habisukan, Ummi Hiras Yustina Hapida, Yustina