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ANOM BOWOLAKSONO
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Indonesia, Kampus Depok, Depok 16424
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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Kelimpahan Sampah Plastik di Pesisir Kota Sorong Provinsi Papua Barat Daya Liling, Irman; Patria, Mufti Petala; Bowolaksono, Anom; Ismail; Supriatna, Jatna; Heatubun, Charlie D.
Igya ser hanjop: Jurnal Pembangunan Berkelanjutan Vol 7 No 1 (2025)
Publisher : Badan Penelitian dan Pengembangan Provinsi Papua Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47039/ish.7.2025.43-51

Abstract

Perkembangan populasi penduduk berbanding lurus dengan peningkatan jumlah sampah atau limbah. Kota Sorong dengan daya tarik sebagai pintu gerbang Papua membuat perkembangan populasinya meningkat pesat. Sampai saat ini, manajemen pengelolaan persampahan di Kota Sorong belum berjalan dengan baik. Pengelolaan sampah di Kota Sorong belum mencakup seluruh wilayah yang ada. Pesatnya perkembangan dan pertumbuhan wilayah menyebabkan sampah yang dihasilkan menjadi besar. Kelimpahan sampah laut terutama jenis plastik menjadi perhatian dunia karena potensi bahaya yang dapat ditimbulkan terhadap kehidupan di bumi. Penelitian ini bertujuan untuk mengetahui kelimpahan sampah plastik di pesisir Kota Sorong. Metode pengambilan sampel dilakukan dua kali secara sengaja menggunakan transek kuadrat (ukuran 2 x 2 m2) pada lima lokasi atau stasiun penelitian. Hasil Penelitian menunjukkan Kelimpahan sampah plastik pada pengambilan sampel I memiliki range antara 0,47 – 20,26 item/m2 dan pada pengambilan sampel II yaitu 0,36 – 13,64 item/m2. Stasiun I memiliki kelimpahan sampah terbesar yaitu 20,26± 16,56 item/m2 (Pengambilan sampel I) dan 13,64± 11,41 item/m2 (Pengambilan sampel II) dan kelimpahan sampah terendah yaitu 0,13± 0,28 item/m2 berlokasi di Stasiun IV pada pengambilan sampel I dan 0,36± 0,48 item/m2 berlokasi di Stasiun V pada pengambilan sampel II. Sampah plastik yang ditemukan didominasi oleh kemasan makanan, serpihan plastik, kemasan plastik dan botol minuman.
Association Between Heat Stress Exposure, Physical Activity, and Nutritional Status with Occupational Fatigue: Pilot Study Kusmawan, David; Perdana, Silvia M.; S., Putri Irwanti; Gustina, Ira; Bowolaksono, Anom; Firmansyah, Ardi
Unnes Journal of Public Health Vol. 14 No. 2 (2025)
Publisher : Universitas Negeri Semarang (UNNES) in cooperation with the Association of Indonesian Public Health Experts (Ikatan Ahli Kesehatan Masyarakat Indonesia (IAKMI))

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/ujph.v14i2.16192

Abstract

Global economic losses from workplace heat and reduced labor productivity are projected to reach nearly US$2 trillion by 2030. The rubber processing industry is particularly vulnerable to heat exposure. This study aimed to describe worker sociodemographic characteristics and assess the relationship between perceived heat stress, nutritional status, physical activity, and occupational fatigue among employees at PT. X in Jambi Province. A cross-sectional design with purposive sampling involved 74 workers across three production areas. Fatigue was measured using the Swedish Occupational Fatigue Inventory (SOFI), while perceived heat stress was assessed with the Heat Stress Perception Index (HSSI). Data were analyzed with univariate and multivariate methods, including multiple linear regression. Participants had a mean age of 34.03 years, BMI of 25.88, and oxygen saturation of 98.01%. The mean heat stress perception score was 50.31, and occupational fatigue averaged 40.55. Lack of energy was the most reported fatigue dimension (mean = 49.23), whereas lack of motivation was the least (mean = 29.92). Regression analysis identified gender (p = 0.003), physical activity (p = 0.031), and perceived heat stress (p = 0.035) as significant predictors. These findings highlight the need for targeted occupational health interventions to mitigate fatigue in the rubber processing industry
Cloning and Expression of SCAMP3 in Escherichia coli BL21(DE3) with In Silico Sequence-Based Cancer Epitopes Prediction Rajagukguk, Selly Setiati; Pambudi, Sabar; Dwiranti, Astari; Utomo, Doddy Irawan Setyo; Bowolaksono, Anom
Makara Journal of Science Vol. 29, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Secretory carrier membrane protein 3 (SCAMP3) is a crucial membrane protein involved in intracellular vesicle traffick-ing and exocytosis. The SCAMP3 expression has been observed in diverse cancer types, such as melanoma, glioma, hepatocellular and breast cancer. Increased SCAMP3 expression has been reported in certain cancer cells relative to that in normal cells, suggesting the potential role of SCAMP3 in cancer development or progression. In this study, we successfully cloned and expressed SCAMP3 in Escherichia coli strain BL21(DE3). SCAMP3 was amplified and insert-ed directionally into the prokaryotic expression vector pET21d(+). The transformation of recombinant plasmid into E. coli BL21(DE3) cells were performed for the protein expression. SDS–PAGE and Western blotting were performed to detect the expression product induced by IPTG, which confirmed the presence of a recombinant pET21d(+)-SCAMP3 at 38-kDa protein weight. Bioinformatics analyses helped discover several possible epitopes distributed throughout the SCAMP3 protein sequence. These findings together serve as a basis for future biochemical and functional studies on this important membrane protein alongside immunotherapy research related to SCAMP3 as a cancer biomarker.
Short Communication: Biometric of freshwater crayfish (Cherax spp.) from Papua and West Papua, Indonesia ABINAWANTO ABINAWANTO; HANI HAMIDAH; ANOM BOWOLAKSONO; RURY EPRILURAHMAN
Biodiversitas Journal of Biological Diversity Vol. 19 No. 2 (2018)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d190216

Abstract

Abinawanto, Hamidah H, Bowolaksono A, Eprilurahman R. 2018. Short Communication: Biometric of freshwater crayfish (Cherax spp.) from Papua and West Papua,, Indonesia. Biodiversitas 19: 489-495. Freshwater crayfish, Cherax spp. is a native crayfish species in Papua, Indonesia. Biometric information of this crayfish species in Indonesia is limited. Hence, this study aimed to provide these data. Specimens were collected from West Papua Province, i.e., from Uter Lake (Atinjo Subdistrict, Maybrat) and Seremuk River (Haha Village, Seremuk Subdistrict, South Sorong), respectively. Besides, the specimens were also collected from Papua Province in Baliem River at Pike Village, Hubukiak Subdistrict, and Wesaput Village, Wesaput Subdistrict, Jayawijaya. Data were analyzed using canonical discriminant function (CDF). The results showed that the longest carapace of Cherax spp. was 97.83 mm found in Baliem River at Pike Village. CDF analysis showed that there were three population groups of Cherax spp. from four locations. The first group originated from Uter Lake (Atinjo Subdistrict) and Seremuk River (Haha village). The second group was from Baliem River (Pike Village; Hubukiak Subdistrict, Jayawijaya and the third group from Baliem River (Wesaput Village; Wesaput Subdistrict)
Short Communication: Ethnozoology – The perception of Tobelo Dalam tribal community in Saolat Village, East Halmahera, Indonesia towards cuscus (Phalangeridae) ELIZABETH NOVI KUSUMANINGRUM; JATNA SUPRIATNA; ABINAWANTO ABINAWANTO; ANOM BOWOLAKSONO
Biodiversitas Journal of Biological Diversity Vol. 19 No. 6 (2018)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d190621

Abstract

Kusumaningrum EN, Supriatna J, Abinawanto, Bowolaksono A. 2018. Short Communication: Ethnozoology – The perception of Tobelo Dalam tribal community in Saolat Village, East Halmahera, Indonesia towards cuscus (Phalangeridae). Biodiversitas 19: 2140-2146. The complexity of the hard lives of Tobelo Dalam tribal community who live in Saolat village, district South Wasile provokes the high activity of animal poaching in Aketajawe-Lolobata National Park (TNAL) including cuscus hunting for both sale and consumption purposes. The study aimed to describe the traditional knowledge of Tobelo Dalam people in Saolat village in applying the systems of utilization and local conservation knowledge towards cuscus. This study was analyzed by the qualitative method with explorative survey technique, interview, and completing questionnaires. This study used 4 key informants (3 males, 1 female) and 50 adult respondents (25 males and 25 females). The results showed that the average percentage of traditional knowledge owned by males and females were 69.1% and 30.9 %, respectively, so the males had an advantage of knowledge around 38.3 % than females in addressing the traditional knowledge of cuscus. The community of Tobelo Dalam tribe hunts cuscus for four different purposes, i.e., for traditional ceremonies, traditional medicine, cuisine, and sell it for more income to support the economy of family.
DNA barcoding reveals underestimated species diversity of mantis shrimp larvae (stomatopods) in Banten Bay, Indonesia ABINAWANTO ABINAWANTO; MARIANA D. BAYU INTAN; WISNU WARDHANA; ANOM BOWOLAKSONO
Biodiversitas Journal of Biological Diversity Vol. 20 No. 6 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d200637

Abstract

Abstract. Abinawanto, Intan MDB, Wardhana W, Bowolaksono A. 2019. DNA barcoding reveals species diversity of mantis shrimp larvae (stomatopods) from Banten Bay, Indonesia. Biodiversitas 20: 1758-1763. Larvae of stomatopods can be morphologically identified by linking between larval and adult form, but it's difficult applied in the laboratory because of requirement of condition to rearing larvae collected either from plankton, or spawning adults in the laboratory. Molecular methods are the alternative approach to gather accurate taxonomic identification. One of molecular methods is DNA barcoding based upon sequence diversity in cytochrome c oxidase subunit 1 (COI). Research on stomatopod larvae diversity and distribution at six stations in Banten Bay has been studied from October 2013 to March 2014. This study was conducted to identify the diversity of stomatopod larvae through DNA barcoding and also to examine the distribution of stomatopod larvae in Banten Bay. DNA barcoding was used to identify mantis shrimp larvae in Banten Bay, Indonesia. Partial mitochondrial COI were obtained for 37 larvae, revealing five distinct molecular operational taxonomic units (MOTUs). All MOTUs distinctively fell into Squillidea clade. Two Molecular Operational Taxonomic Units, MOTUs were successfully identified to species level (i.e. Anchisquilla fasciata), while three others remained identified to genus level (i.e. Stomatopoda spp). The intraspecific variation of COI ranged from 0% to 3.1% (Kimura 2-parameter distance), whereas interspecific divergence ranged from 5.9% to 20.4%. These results imply that COI genes are suitable for species identification of stomatopod larvae in this area, though the number of unidentified species was higher than the identified ones. This is likely because of the limited database in GenBank. In addition, the two identified species represents new records for this area, suggesting that the mantis shrimp in the area have been underestimated based on traditional biodiversity measures. We recommend to develop a taxonomically-comprehensive DNA barcoding database to improve the accuracy and feasibility of using DNA barcoding for species identification of Indonesia mantis shrimps.
Analysis of IGF-1 gene in ayam ketawa (Gallus gallus domesticus) with dangdut and slow type vocal characteristics ABINAWANTO ABINAWANTO; ALFI SOPHIAN; RETNO LESTARI; ANOM BOWOLAKSONO; PIPIH SUNINGSIH EFENDI; RUDI AFNAN
Biodiversitas Journal of Biological Diversity Vol. 20 No. 7 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d200729

Abstract

Abstract. Abinawanto, Sophian A, Lestari R, Bowolaksono A, Efendi PS, Afnan R. 2019. Analysis of IGF-1 gene in ayam ketawa (Gallus gallus domesticus) with dangdut and slow type vocal characteristics. Biodiversitas 20: 2004-2010. Insulin-like growth factor 1 (IGF-1) is defined as an essential gene for growth. Therefore, this research aims to identify the polymorphism of the IGF-1 gene from ayam ketawa with the dangdut and slow type vocal characteristics. Twenty male chickens were selected as an animal model. The sample consisted of 10 chickens with the dangdut type and 10 chickens with the slow type. The chickens were all obtained from five areas of the Pinrang district. The RFLP and HRM methods were selected to be used in this research. Results showed that the IGF-1 gene was 622 bp in length, while polymorphism analysis showed that the dangdut and slow type are homozygotic. The unrestricted DNA fragment indicated it after reaction of the restriction enzyme Pst1. Moreover, it confirmed that ayam ketawa with the dangdut or slow type had a similar sequence.
The genetic diversity of ayam ketawa (Gallus gallus domesticus, Linneaus, 1758) in Bangkalan District, Madura Island, Indonesia ABINAWANTO Abinawanto; TRI ZULISTIANA; RETNO LESTARI; ASTARI DWIRANTI; ANOM BOWOLAKSONO
Biodiversitas Journal of Biological Diversity Vol. 22 No. 6 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220617

Abstract

Abstract. Abinawanto, Zulistiana T, Lestari R, Dwiranti A, Bowolaksono A. 2021. The genetic diversity of ayam ketawa (Gallus gallus domesticus, Linneaus, 1758) in Bangkalan District, Madura Island, Indonesia. Biodiversitas 22: 3145-3155. Ayam Ketawa, a variation of Gallus gallus domesticus Gallus gallus domesticus, comes from  Sidendreng-Rappang (Sidrap) District, South Sulawesi, Indonesia. Apart from Sidrap, ayam ketawa farms are also found in Bangkalan District, on the island of Madura, Indonesia. Ayam ketawa from Bangkalan District often wins national level contests. Therefore, the research was carried out with the aim of knowing the genetic diversity of ayam ketawa in Kamal Subdistrict, Socah Subdistrict, and Burneh Subdistrict, based on bioacoustic, morphometric, and barcoding DNA analysis. The studied ayam ketawa consists of dangdut and slow types. The longest duration (6.00 ± 3.0) seconds was shown by ayam ketawa from Kamal Subdistrict, while the highest number of syllables (7.20 ± 5.80) came from Socah Subdistrict. The results of the Mann-Whitney test showed that eight morphological characters were significantly different (P <0.05) between ayam ketawa population in Kamal and Burneh subdistricts, namely body weight, femur length, shank length, shank circumference, wing length, comb height, third finger length, and breastbone length. Four morphological characters differed significantly (P <0.05) between ayam ketawa population in Kamal and Socah subdistricts, namely shank length, wing length, third finger length, and sternum length. Three morphological characters were significantly different (P <0.05) between the Burneh and Socah subdistricts population, namely body weight, femur length, and shank circumference. The analysis results of DNA Barcoding gene of the Cytochrome Oxidase subunit 1 (CO1) showed a genetic distance ranging from 0.025 to 1.872.
Urine biochemical profile analysis and transabdominal ultrasonography for pregnancy detection in the endangered lowland anoa (Bubalus depressicornis) Anom Bowolaksono; Anita Mayasari; Ayu Mulia Sundari; Advent Tri Joy Simamora; Rahma Suryaningsih; Ady Suryawan; Abinawanto Abinawanto; Luthfiralda Sjahfirdi; Afifa Hasna; Jatna Supriatna; Randall C Kyes
Biodiversitas Journal of Biological Diversity Vol. 23 No. 11 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d231161

Abstract

Abstract. Bowolaksono A, Mayasari A, Sundari AM, Simamora ATJ, Suryaningsih R, Suryawan A, Simamora ATJ, Abinawanto, Sjahfirdi L, Hasna A, Supriatna J, Kyes RC. 2022. Urine biochemical profile analysis and transabdominal ultrasonography for pregnancy detection in the endangered lowland anoa (Bubalus depressicornis). Biodiversitas 23: 6056-6061. The population of lowland anoas (Bubalus depressicornis) has decreased significantly due to illegal hunting and habitat loss. As such, the urgency in developing successful ex-situ conservation programs cannot be overstated. Ex-situ breeding programs of anoas, however, are hindered by difficulties in early pregnancy detection. The failure to detect pregnancy at an early stage often results in miscarriage due to repeated breeding attempts of unconfirmed pregnant females. Moreover, frequent false heat during 5 to 6 months of pregnancy corroborated the repeated breeding of pregnant females. Therefore, early detection techniques are needed to improve breeding success. The present study evaluated the use of urine biochemical profiles as a minimally invasive approach for early pregnancy detection and transabdominal ultrasonography as a confirmation tool of false heat in four lowland anoas. Statistical analysis showed that glucose and protein level were significantly elevated during pregnancy since the 0 days of post-mating (p<0.05) and consistently higher compared to the non-pregnant period. Meanwhile, transabdominal ultrasonography successfully identified organ structures and development by the 155th, 176th, 180th, and 191st days of pregnancy. The present study suggests that urine biochemical data provide a promising, minimally invasive approach to early pregnancy detection in anoas. Incorporating urine data and ultrasonography can significantly help to maintain pregnancy by validating false heat.
The effect of different honey concentrations on the ultrastructure profile of spermatozoa in Dewa Mahseer (Neolissochilus soro) ABINAWANTO ABINAWANTO; SUCI LESTARI; ANOM BOWOLAKSONO; ASTARI DWIRANTI; RETNO LESTARI; RUDHY GUSTIANO; ANANG HARI KRISTANTO
Biodiversitas Journal of Biological Diversity Vol. 24 No. 2 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240243

Abstract

Abstract. Abinawanto A, Lestari S, Bowolaksono A, Dwiranti A, Lestari R, Gustiano R, Kristanto AH. 2023. Title The effect of different honey concentrations on the ultrastructure profile of spermatozoa in Dewa Mahseer (Neolissochilus soro). Biodiversitas 24: 1025-1031. Dewa Mahseer (Neolissochilus soro) is one of Indonesia's endemic freshwater fish at risk of population decline due to water pollution and asynchronous maturation of gonads. One of the efforts to conserve this species is preserving their spermatozoa using honey as a protector at certain temperatures. Therefore, this study aims to evaluate the effect of multiflora honey concentrations on ultrastructure spermatozoa profile of Dewa Mahseer. The temperature was maintained at 4°C and stored for 48 hours, followed by the cement dilution (1:10) in a stock solution containing fish ringer and honey. Transmission electron microscopy (TEM) examined the spermatozoa profiles at a magnification of ×1,500. Furthermore, ANOVA one-way analysis of variance was conducted to test the effect of head length, width, and area of spermatozoa on honey. The results showed a statistically significant difference in head width (F=13.929, p=0.001). Also, there is a difference in area between honey spermatozoa, fresh spermatozoa, and without honey at a cold of 4°C. The fresh spermatozoa stored at cold temperatures without honey observed considerable membrane damage. The novel aspect is an investigation of the effect of honey as a natural protector in the storage of Neolissochilus spermatozoa at 4°C for 48 hours. This study can be applied as a guide for the 48 hours of spermatozoa in a cold temperature in fish farming.
Co-Authors Abinawanto Advent Tri Joy Simamora Ady Suryawan Afifa Hasna Ahmad Aulia Jusuf ALFI SOPHIAN Amin Soebandrio Amira, Qanita Hana ANANG HARI KRISTANTO Anita Mayasari Arief Boediono Asmarinah Astari Dwiranti Aucky Hinting Ayu Mulia Sundari Ayu Mulia Sundari BENEDIKTUS YOHAN David Kusmawan DENDI HADI PERMANA DIN SYAFRUDDIN Dionisius Denis DIONISIUS DENIS ELIZABETH NOVI KUSUMANINGRUM Fadhilah Fadilah Firmansyah, Ardi Funahashi, Hiroaki Gustina, Ira HANI HAMIDAH Heatubun, Charlie D. Hiroaki Funahashi Huriyah Adani Saoemi Ismail ISMAIL EKOPRAYITNO ROZI Jatna Supriatna Justus Sievers LEPA SYAHRANI Lia Amelia Tresna Wulan Asri Liling, Irman Lisya Mutiara Dewi LUTHFIRALDA SJAHFIRDI MARIANA D. BAYU INTAN Moch Saifur Rijal Moudy Soraya Mufti Petala Patria Nova Anita Nurul Muhammad Prakoso Oktaviani Naulita Turnip Perdana, Silvia M. Petrus Supardi PIPIH SUNINGSIH EFENDI Prakoso, Nurul Muhammad PUJI BUDI SETIA ASIH Putri Irwanti Sari R. Tedjo Sasmono R. TEDJO SASMONO RAHMA F. HAYATI Rahma Suryaningsih Rajagukguk, Selly Setiati Randall C Kyes RETNO LESTARI RETNO LESTARI RETNO LESTARI Retno Lestari RIZKA ALAWIYAH RUDHY GUSTIANO Rudi Afnan RURY EPRILURAHMAN Sabar Pambudi Setiorini, Setiorini Silvia werdhy lestari Siti Z Musthofa SUCI LESTARI Sundari, Ayu Mulia TRI ZULISTIANA Tri Aprilliana Wulandari Utomo, Doddy Irawan Setyo WISNU WARDHANA