Article Per Year (5 Year)
p-Index From 2021 - 2026
1.532
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Microbiology Indonesia Media Penelitian dan Pengembangan Kesehatan Jurnal Sain Peternakan Indonesia Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Current Research on Biosciences and Biotechnology Igya ser hanjop: Jurnal Pembangunan Berkelanjutan Makara Journal of Science Journal of Global Pharma Technology Unnes Journal of Public Health
ANOM BOWOLAKSONO
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Indonesia, Kampus Depok, Depok 16424
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

Published : 14 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 14 Documents
Search

 1   2 
Caffeine as a modulator of redox balance and migration in MDA-MB-231 triple-negative breast cancer cells Amira, Qanita Hana; Fadilah; Bowolaksono, Anom; Dwiranti, Astari
Current Research on Biosciences and Biotechnology Vol. 6 No. 2 (2025)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2025.6.2/54S7U9DO

Abstract

Caffeine, a widely consumed stimulant, has demonstrated significant effects on cancer cell behavior, particularly in triple-negative breast cancer (TNBC) cells. This study investigates the impact of caffeine on MDA-MB-231 cells, focusing on cell morphology, viability, antioxidant gene expression, and cell migration. Caffeine at concentrations of 10 mM and above induced notable morphological changes, including cell rounding, detachment, and decreased cell density, indicative of cytotoxic effects and cellular stress responses such as apoptosis. Viability assays revealed a dose-dependent reduction in cell survival, with a substantial decline in total cell count at higher caffeine concentrations. The observed decrease in cell viability is associated with the downregulation of antioxidant genes SOD2 and GLO1, suggesting disrupted redox balance and impaired detoxification systems. A significant positive correlation was noted between SOD2 and GLO1 expression levels, indicating their interdependence in antioxidant defense mechanisms. Additionally, caffeine treatment impaired cell migration, as the wound healing assay shows, with higher concentrations significantly hindering wound closure. This effect on migration, relevant for metastasis, aligns with findings from previous studies on caffeine’s influence on cancer cell motility. The results suggest that 10 mM caffeine may serve as an optimal concentration for inducing cellular stress without immediate, widespread cell death, positioning it as a promising candidate for further therapeutic exploration.
Kelimpahan Sampah Plastik di Pesisir Kota Sorong Provinsi Papua Barat Daya Liling, Irman; Patria, Mufti Petala; Bowolaksono, Anom; Ismail; Supriatna, Jatna; Heatubun, Charlie D.
Igya ser hanjop: Jurnal Pembangunan Berkelanjutan Vol 7 No 1 (2025)
Publisher : Badan Penelitian dan Pengembangan Provinsi Papua Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47039/ish.7.2025.43-51

Abstract

Perkembangan populasi penduduk berbanding lurus dengan peningkatan jumlah sampah atau limbah. Kota Sorong dengan daya tarik sebagai pintu gerbang Papua membuat perkembangan populasinya meningkat pesat. Sampai saat ini, manajemen pengelolaan persampahan di Kota Sorong belum berjalan dengan baik. Pengelolaan sampah di Kota Sorong belum mencakup seluruh wilayah yang ada. Pesatnya perkembangan dan pertumbuhan wilayah menyebabkan sampah yang dihasilkan menjadi besar. Kelimpahan sampah laut terutama jenis plastik menjadi perhatian dunia karena potensi bahaya yang dapat ditimbulkan terhadap kehidupan di bumi. Penelitian ini bertujuan untuk mengetahui kelimpahan sampah plastik di pesisir Kota Sorong. Metode pengambilan sampel dilakukan dua kali secara sengaja menggunakan transek kuadrat (ukuran 2 x 2 m2) pada lima lokasi atau stasiun penelitian. Hasil Penelitian menunjukkan Kelimpahan sampah plastik pada pengambilan sampel I memiliki range antara 0,47 – 20,26 item/m2 dan pada pengambilan sampel II yaitu 0,36 – 13,64 item/m2. Stasiun I memiliki kelimpahan sampah terbesar yaitu 20,26± 16,56 item/m2 (Pengambilan sampel I) dan 13,64± 11,41 item/m2 (Pengambilan sampel II) dan kelimpahan sampah terendah yaitu 0,13± 0,28 item/m2 berlokasi di Stasiun IV pada pengambilan sampel I dan 0,36± 0,48 item/m2 berlokasi di Stasiun V pada pengambilan sampel II. Sampah plastik yang ditemukan didominasi oleh kemasan makanan, serpihan plastik, kemasan plastik dan botol minuman.
Association Between Heat Stress Exposure, Physical Activity, and Nutritional Status with Occupational Fatigue: Pilot Study Kusmawan, David; Perdana, Silvia M.; S., Putri Irwanti; Gustina, Ira; Bowolaksono, Anom; Firmansyah, Ardi
Unnes Journal of Public Health Vol. 14 No. 2 (2025)
Publisher : Universitas Negeri Semarang (UNNES) in cooperation with the Association of Indonesian Public Health Experts (Ikatan Ahli Kesehatan Masyarakat Indonesia (IAKMI))

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/ujph.v14i2.16192

Abstract

Global economic losses from workplace heat and reduced labor productivity are projected to reach nearly US$2 trillion by 2030. The rubber processing industry is particularly vulnerable to heat exposure. This study aimed to describe worker sociodemographic characteristics and assess the relationship between perceived heat stress, nutritional status, physical activity, and occupational fatigue among employees at PT. X in Jambi Province. A cross-sectional design with purposive sampling involved 74 workers across three production areas. Fatigue was measured using the Swedish Occupational Fatigue Inventory (SOFI), while perceived heat stress was assessed with the Heat Stress Perception Index (HSSI). Data were analyzed with univariate and multivariate methods, including multiple linear regression. Participants had a mean age of 34.03 years, BMI of 25.88, and oxygen saturation of 98.01%. The mean heat stress perception score was 50.31, and occupational fatigue averaged 40.55. Lack of energy was the most reported fatigue dimension (mean = 49.23), whereas lack of motivation was the least (mean = 29.92). Regression analysis identified gender (p = 0.003), physical activity (p = 0.031), and perceived heat stress (p = 0.035) as significant predictors. These findings highlight the need for targeted occupational health interventions to mitigate fatigue in the rubber processing industry
Cloning and Expression of SCAMP3 in Escherichia coli BL21(DE3) with In Silico Sequence-Based Cancer Epitopes Prediction Rajagukguk, Selly Setiati; Pambudi, Sabar; Dwiranti, Astari; Utomo, Doddy Irawan Setyo; Bowolaksono, Anom
Makara Journal of Science Vol. 29, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Secretory carrier membrane protein 3 (SCAMP3) is a crucial membrane protein involved in intracellular vesicle traffick-ing and exocytosis. The SCAMP3 expression has been observed in diverse cancer types, such as melanoma, glioma, hepatocellular and breast cancer. Increased SCAMP3 expression has been reported in certain cancer cells relative to that in normal cells, suggesting the potential role of SCAMP3 in cancer development or progression. In this study, we successfully cloned and expressed SCAMP3 in Escherichia coli strain BL21(DE3). SCAMP3 was amplified and insert-ed directionally into the prokaryotic expression vector pET21d(+). The transformation of recombinant plasmid into E. coli BL21(DE3) cells were performed for the protein expression. SDS–PAGE and Western blotting were performed to detect the expression product induced by IPTG, which confirmed the presence of a recombinant pET21d(+)-SCAMP3 at 38-kDa protein weight. Bioinformatics analyses helped discover several possible epitopes distributed throughout the SCAMP3 protein sequence. These findings together serve as a basis for future biochemical and functional studies on this important membrane protein alongside immunotherapy research related to SCAMP3 as a cancer biomarker.
Co-Authors Abinawanto Abinawanto Abinawanto Ahmad Aulia Jusuf Amin Soebandrio Amira, Qanita Hana Arief Boediono Asmarinah Asmarinah Aucky Hinting BENEDIKTUS YOHAN David Kusmawan DIONISIUS DENIS Dionisius Denis Dwiranti, Astari Fadhilah Fadilah Firmansyah, Ardi Funahashi, Hiroaki Gustina, Ira Heatubun, Charlie D. Huriyah Adani Saoemi Ismail Jatna Supriatna Justus Sievers Liling, Irman Moudy Soraya Mufti Petala Patria Nova Anita Oktaviani Naulita Turnip Pambudi, Sabar Perdana, Silvia M. Petrus Supardi Prakoso, Nurul Muhammad R. TEDJO SASMONO R. Tedjo Sasmono RAHMA F. HAYATI Rajagukguk, Selly Setiati Retno Lestari RIZKA ALAWIYAH S., Putri Irwanti Setiorini, Setiorini silvia werdhy lestari Siti Z Musthofa Sundari, Ayu Mulia Utomo, Doddy Irawan Setyo Wulandari, Tri Aprilliana