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BIOACTIVITY OF ENDOPHYTIC FUNGI EXTRACT ISOLATED FROM THE LEAVES OF MISTLETOE (Dendrophthoe pentandra (L.) Miq.) ON THE LIME PLANT (Citrus aurantifolia) Hiras Habisukan, Ummi; Oktiansyah, Rian; Noviyanto; Anjeli, Riri
Berita Biologi Vol 23 No 3 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2024.7423

Abstract

Mistletoe (Dendrophthoe pentandra (L.) Miq.) is a parasitic plant used in traditional medicine. This study evaluates the bioactivity of endophytic fungi in mistletoe . Endophytic fungal species were determined through morphological identification. Potato Dextrose Broth (PDB) media was used for the cultivation, and ethyl acetate was used as solvent to extract secondary metabolites. The antioxidant test was carried out using the DPPH method, while the paper disc diffusion method performed the antibacterial test. A total of 4 isolates of endophytic fungi were obtained from mistletoe leaves, namely isolates DB1 – DB4. The results of morphological analysis showed that DB1 was Paecilomyces sp., DB2 was Papulaspora sp., DB3 was Aspergillus sp., and DB4 was Mucor sp. The endophytic fungus DB3 (Aspergillus sp.) showed the most potential antioxidant and antibacterial activity. This endophytic fungal extract can potentially be a source of new drugs through further research by isolating the active compound.  
BIOACTIVITY OF ENDOPHYTIC FUNGI EXTRACTS RESIDING IN THE ROOTS OF MISTLETOE (Dendrophthoe pentandra) Oktiansyah, Rian; Habisukan, Ummi Hiras; Noviyanto, Noviyanto; Nasution, Sakinah Salman Ahmad; Febiantika, Imaniar; Octavia, Risky
Berita Biologi Vol 24 No 1 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Mistletoe (Dendrophthoe pentandra) is a parasitic plant that has traditional medicinal properties. This study examines the endophytic fungi found in mistletoe roots and their bioactivity. Endophytic fungal species are identified using their morphological traits. Potato Dextrose Broth (PDB) medium is utilized for both culture and extraction, with ethyl acetate as a solvent. The antioxidant test employed the DPPH method, whereas the antibacterial test used the paper disc diffusion method. Mistletoe roots yielded four endophytic fungus isolates (BR1–BR4). The findings of morphological study revealed that BR1 (Pythium sp.), BR2 (Trichoderma sp.), BR3 (Fusarium sp.), and BR4 (Mortierella sp.). The endophytic fungus BR4 (Mortierella sp.) had the highest potential antioxidant and antibacterial activities. This endophytic fungal extract has the potential to be a source of novel medications through additional investigation by isolating the pure component and in vivo studies.
Bird diversity in the Mount Dempo area, South Sumatra: An ecological resource for local-based biology learning Armanda, Fahmy; Wicaksono, Anggun; Oktiansyah, Rian
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 02 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i02.2031

Abstract

Mount Dempo, South Sumatra, hosted a diverse range of bird species, making it a valuable ecological resource for local-based biology learning. This study aimed to document bird diversity in the region and to explore its potential for ecological education. The research was conducted in 2020 during the COVID-19 pandemic era. Data collection was carried out in the Mount Dempo area, limited to an altitude of approximately 2,086 meters above sea level at Shelter 1. The research employed the observation (point count) method along ecotourism routes by recording trails traversed using GPS. Sampling was conducted by walking along the path accompanied by predetermined observation points. The results recorded 21 bird species with a total of 96 individuals. The diversity analysis using the Shannon-Wiener Index produced a value of H' = 2.68, indicating moderate to high diversity, while the Simpson’s Index (D = 0.092) suggested very low species dominance. Additionally, the Evenness Index (E = 0.88) reflected a relatively even distribution of individuals among species, highlighting ecological stability in the study area. The presence of various ecological niches and bird functional groups supported the development of environmental literacy and conservation awareness among students. Thus, integrating local bird diversity as a contextual learning resource was considered a strategic approach to enhance ecological understanding and foster pro-environmental behavior among learners.