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All Journal AGRIVITA, Journal of Agricultural Science Jurnal Dinamika Pengabdian (JDP) International Journal of Agriculture System JURNAL AGRIVIGOR
Haring, Feranita
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Agriculture, Biological Sciences & Forestry

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PERBENIHAN KULTUR JARINGAN ANGGREK PADA TEACHING INDUSTRY UNIVERSITAS HASANUDDIN Sjahril, Rinaldi; Haring, Feranita; Rukka, Rusli M.; Dermawan, Rahmansyah
Jurnal Dinamika Pengabdian Vol. 4 No. 2 (2019): JURNAL DINAMIKA PENGABDIAN VOL. 4 NO. 2 MEI 2019
Publisher : Departemen Budidaya Pertanian Fakultas Pertanian UNHAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jdp.v4i2.7415

Abstract

Kegiatan PPMU-PPUPIK Perbenihan Kultur Jaringan Anggrek pada Teaching Industry Universitas Hasanuddin (UNHAS) bertujuan untuk mempercepat proses pengembangan budaya kewirausahaan, mendorong berkembangnya budaya pemanfaatan hasil riset UNHAS bagi masyarakat, dan menunjang otonomi kampus melalui perolehan pendapatan mandiri atau bermitra. Dalam pencapaian tujuan jangka panjangnya, kegiatan ini dalam jangka pendek bertujuan untuk memberikan kesempatan dan pengalaman kerja kepada mahasiswa, dan membantu menciptakan akses bagi terciptanya wirausaha baru. Untuk mencapai tujuan tersebut dilakukan kegiatan perbanyakan semaian biji dan bibit anggrek spesies/hibrida di laboratorium, dan pelatihan tentang teknik kultur jaringan skala rumah tangga dimana diajarkan tentang penyederhanaan teknik kultur jaringan tanaman sehingga dapat dilakukan pada skala rumah tangga. Kegiatan laboratorium dan pelatihan dilaksanakan di Teaching Industry UNHAS dan bermitra dengan pengusaha tanaman hias lokal yang berada di Makassar yang memiliki kebun anggrek di Bulu Dua, Kabupaten Barru. Produksi (production planning) yang dilakukan adalah semaian bibit botol/semai biji anggrek disubkultur dan dilakukan pemeliharaan sampai menjadi dewasa dan siap untuk aklimatisasi. Kemudian planlet yang telah diaklimatisasi dirawat dan dipelihara di screen house sampai menjadi anggrek dewasa yang siap jual ke pasaran. Selain itu planlet yang diperbanyak di laboratorium juga telah dipasarkan. Hasil penjualan anggrek yang paling digemari konsumen adalah Seedling Dendrobium dan Phalaenopsis Hibrida serta anggrek Macodes. Pelatihan kultur jaringan sederhana dilakukan dengan pengenalan budidaya anggrek dan teknik kultur jaringan sederhana, perakitan alat kultur jaringan sederhana, praktek teknik kultur jaringan dimulai dari pembuatan media, penanaman, sampai pada aklimatisasi. Dari hasil pelatihan yang dilaksanakan dapat disimpukan bahwa kultur jaringan tidak mahal dan rumit, serta dapat disederhanakan dengan teknik-teknik yang mudah dipahami peserta.Kata kunci: anggrek, kultur jaringan sederhana, pelatihan, perbenihan
Callus Induction of Porang Plants (Amorphophallus muelleri Blume) with 2,4- D on Various Explant Sources In Vitro Haring, Feranita; Sjahril, Rinaldi; Khatima, Khusnul
International Journal of Agriculture System VOLUME 11 ISSUE 2, DECEMBER 2023
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijas.v11i2.4796

Abstract

This study aims to determine the effect of a concentration of 2,4-D added to the Murhasige and Skoog (MS) basic media on porang callus induction and the effect on various explant sources. The study was conducted in the form of experiments in the Laboratory of Bioscience and Plant Reproduction Biotechnology, Department of Agriculture, Faculty of Agriculture, Hasanuddin University from August to December 2021. The experiments were based on a Completely Randomized Design (CRD) pattern arranged in a factorial way with two treatment factors. The first factor was the concentration of 2,4-D consisting of five levels of treatment: without 2,4-D (0.0 mg L-1), 0.5 mg L-1, 1.0 mg L-1, 1.5 mg L-1, and 2.0 mg L-1. The second factor was the source of explants consisting of three levels: the petiole, leaf midrib, and leaf blade. The results after five months showed that the interaction between the concentration of 2.0 mg L-1 2,4-D with the petiole explants had the best effect on callus induction time (12.25 days) and callus weight (2.97 g). The concentration of 2.0 mg L-1 2,4-D had the best influence on the callus induction percentage (83.33%), and petiole-derived explants had the best influence on the callus induction percentage (91.67%). Administration of 2,4-D at a concentration of 2.0 mg L-1 and the petiole-derived explant gave the best results in this study; therefore, it is expected to be applied to the development of porang plant seeds in vitro.
Molecular Diversity Analysis of Katokkon Chili (Capsicum annum var. chinense) Based on Inter Simple Sequence Repeat (ISSR) Markers Al Amanah, Hafizhah; Sjahril, Rinaldi; Haring, Feranita; Larekeng, Siti Halimah
AGRIVITA Journal of Agricultural Science Vol 46, No 2 (2024)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v46i2.3997

Abstract

There are numerous kinds of chilies, and many have a specialty and specific growing location, such as the katokkon chili, which is specific to Toraja Highland, South Sulawesi Province, Indonesia. This small bell-chili pepper-shaped chili has a distinctive capsicum aroma and a short burning sensation of hot chili's strong spicy taste. This research aims to study the relationship of the genetic kinship of katokkon chili from Toraja and its surrounding regency (Enrekang and Mamasa regencies) using ISSR molecular markers, which are expected to be used as basic information in genetic resource management. Inter Simple Sequences Repeat (ISSR) markers are used for the molecular analysis of 26 katokkon accessions. The result shows that katokkon chili analyzed have a relatively high heterozygosity value of 0.488. The average heterozygosity value is 0.5. Hence, the genetic diversity in these districts is comparatively high. The katokkon chili accession is grouped into three main clusters, each comprising various samples. Only clusters II and III have a close relationship. Information on the genetic diversity based on ISSR can be used as a molecular marker to support further research on the breeding and development of katokkon chili plants and their accession.
Co-Authors . Jamila, . . Mufidah Al Amanah, Hafizhah Amin, A Rusdayani Aminah, St.Aminah AMIRULLAH DACHLAN Asis, Baharuddin Astina Tambung, Astina Dermawan, Rahmansyah Dungga, Novaty Eny Ifayanti Ridwan, Ifayanti Iswoyo, Hari Khatima, Khusnul Muhammad Nasrun Nurlina Kasim Putri, Nur Hilmih Disya Rafiuddin, . Riadi, Muh. Rinaldi Sjahril, Rinaldi Rukka, Rusli M. Sadli, Rahmat Sahur, Asmiaty SITI HALIMAH LAREKENG, SITI HALIMAH Syaiful, Syatrianty A Syam'un, Elkawakib SYATRIANTY A. SYAIFUL Ulfa, Fachirah