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Development of a natural eosinophil stain based on cordyline fruticosa leaves extract and selenicereus monacanthus (dragon fruit peel extracts) for hematology diagnostic applications Hasanah, Annisa Nur; Liswanti, Yane; Kusmiati, Meti; Salsabila, Aliya Fitria; Prihartini, Linda
Science Midwifery Vol 13 No 6 (2026): February: Health Sciences and related fields
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v13i6.2268

The search for natural alternatives to conventional hematological stains has gained attention due to issues of dependency on imported reagents, cost, and potential environmental impact. This study aimed to explore the potential of Cordyline fruticosa (andong leaves) and Selenicereus monacanthus (dragon fruit peel) extracts as natural eosinophil stains in hematology. Pigment extraction was performed using standard procedures, followed by phytochemical screening for anthocyanins and flavonoids. The total anthocyanin content was higher in C. fruticosa (1.20 mg/L) compared to S. monacanthus (0.30 mg/L), while relative flavonoid absorbance values also indicated greater pigment density in andong leaves. Application of the extracts in the hemocytometer method demonstrated staining of the background and cellular components, although with weaker intensity than conventional eosin. In peripheral blood smear preparations, Giemsa stain produced the most distinct results, clearly differentiating eosinophil nuclei, cytoplasm, and characteristic granules. In contrast, the natural extracts yielded paler orange cream staining, with less clarity and limited granule visualization, and some smears exhibited detachment due to imperfect fixation. These findings suggest that while andong leaves and dragon fruit peel contain bioactive pigments with staining potential, their application in hematology requires further optimization, particularly in fixation methods, extract concentration, and stability testing over longer storage periods.

Pengaruh Literasi Keuangan Digital Dan Perilaku Penggunaan Platform Fintech Terhadap Resiliensi Keuangan Keluarga ASN: Peran Mediasi Loyalitas Platform Alyssa, Heidy Puspa; Bidhari, Sandhika Cipta; Hasanah, Annisa Nur
Manajemen: Jurnal Ekonomi Vol. 8 No. 1 (2026): Manajemen: Jurnal Ekonomi Vol 8 No 1 Maret 2026
Publisher : Fakultas Ekonomi Universitas Simalungun

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36985/yppvzj68

Penelitian ini mengkaji pengaruh literasi keuangan digital terhadap resiliensi keuangan keluarga Aparatur Sipil Negara (ASN) melalui peran perilaku penggunaan platform teknologi finansial dan loyalitas platform. Penelitian menggunakan pendekatan survei kuantitatif terhadap 30 anggota Dharma Wanita Persatuan yang berafiliasi dengan Dinas Sosial, Pemberdayaan Perempuan, dan Perlindungan Anak Kabupaten Mojokerto. Seluruh responden merupakan perempuan yang berperan sentral dalam pengelolaan keuangan keluarga dan secara aktif menggunakan minimal dua aplikasi fintech. Analisis data dilakukan menggunakan Partial Least Squares–Structural Equation Modeling (PLS-SEM) dengan prosedur bootstrapping sebanyak 5.000 resampling. Hasil penelitian menunjukkan bahwa literasi keuangan digital berpengaruh signifikan dan kuat terhadap perilaku penggunaan platform fintech (β = 0,852; t = 27,649; p < 0,001). Namun, literasi keuangan digital tidak berpengaruh langsung secara signifikan terhadap resiliensi keuangan keluarga (β = 0,203; t = 0,983; p = 0,163). Perilaku penggunaan platform fintech berpengaruh signifikan terhadap loyalitas platform (β = 0,514; t = 4,641; p < 0,001), sedangkan loyalitas platform tidak berpengaruh signifikan terhadap resiliensi keuangan keluarga dan menunjukkan arah hubungan negatif (β = –0,079; t = 0,298; p = 0,383). Sebaliknya, variabel kontrol berupa tingkat pendapatan keluarga memiliki pengaruh positif dan signifikan terhadap resiliensi keuangan keluarga (β = 0,495; t = 3,380; p < 0,001). Temuan ini menunjukkan bahwa literasi keuangan digital lebih berperan dalam membentuk perilaku penggunaan fintech dibandingkan secara langsung meningkatkan resiliensi keuangan, serta mengindikasikan adanya potensi loyalty paradox dalam pemanfaatan platform fintech oleh keluarga ASN

SDS-PAGE characterization of dialyzed protein fractions from mycobacterium tuberculosis isolates for tuberculosis biomarker screening Barokah, Githa Agripina; Novitriani, Korry; Hasanah, Annisa Nur; Ferdiani, Dina; Setyoningrum, Fransisca Probo
Science Midwifery Vol 14 No 1 (2026): April: Health Sciences and related fields
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v14i1.2333

Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis, in which the identification of protein profiles serves as an important approach in diagnostic research and specific antigen development. This study aimed to analyze the protein profile of dialyzed fractions from Mycobacterium tuberculosis isolates using the SDS-PAGE (Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis) method. Protein isolation was carried out through sonication at 4 °C to lyse the cells and release intracellular proteins without causing denaturation. The resulting lysate supernatant was subjected to 30% ammonium sulfate precipitation to separate proteins based on their solubility, followed by dialysis to remove residual salts and unwanted small molecules. The total protein concentration was measured using a photometer with a total protein reagent, showing a relatively low value, indicating that the protein fraction was concentrated on specific molecules with higher purity. The SDS-PAGE analysis revealed the presence of a protein band with an estimated molecular weight of 77,6 kDa, suggesting the successful purification of protein fractions based on molecular weight. Based on these findings, it can be concluded that the combination of sonication, ammonium sulfate precipitation, and dialysis is effective as a preliminary purification method to obtain purer proteins from Mycobacterium tuberculosis isolates and enables more specific characterization of protein profiles through SDS-PAGE.

Learning Culture of Elementary School Students: An Ethnographic Study at MI Nurul Huda Bengkulu City Fitri, Nabilah Aisyah; Hasanah, Annisa Nur; Friantary, Henny; Ahmad, Pahrozi; Yulizah, Yosi
International Journal on Advanced Science, Education, and Religion Vol 8 No 1 (2025): IJoASER (International Journal on Advanced Science, Education)
Publisher : Sekolah Tinggi Agama Islam Al-Furqan, Makassar - Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33648/ijoaser.v8i1.1605

Learning culture is an important factor in determining the success of the learning process in elementary schools. This study aims to describe students learning culture and analyze the factors that influence it at MI Nurul Huda, Bengkulu City. This study used a qualitative approach with an ethnographic design. Data collection techniques were carried out through participatory observation, in-depth interviews, and documentation. Data analysis was conducted using an interactive model that includes data reduction, data presentation, and conclusion drawing. The results of the study indicate that students learning culture is formed through four main themes: discipline, learning habits, social interaction, and learning motivation. Studfents learning culture has generally developed well, especially in aspects of discipline and social interaction, but is not evenly distributed in the aspect of independent learning habits. The role of teachers has proven to be a key factor in shaping a learning culture through learning strategies and classroom management, while the social and cultural environment also influences students learning habits. The findings of this study indicate that learning culture is the result of an on going interaction between internal and external factors of students. Systematic efforts are needed through culture-based learning, strengthening the role of teachers, and environmental support to improve students learning culture

Learning Culture of Elementary School Students: An Ethnographic Study at MI Nurul Huda Bengkulu City Fitri, Nabilah Aisyah; Hasanah, Annisa Nur; Friantary, Henny; Ahmad, Pahrozi; Yulizah, Yosi
International Journal on Advanced Science, Education, and Religion Vol 8 No 1 (2025): IJoASER (International Journal on Advanced Science, Education)
Publisher : Sekolah Tinggi Agama Islam Al-Furqan, Makassar - Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33648/ijoaser.v8i1.1605

Learning culture is an important factor in determining the success of the learning process in elementary schools. This study aims to describe students learning culture and analyze the factors that influence it at MI Nurul Huda, Bengkulu City. This study used a qualitative approach with an ethnographic design. Data collection techniques were carried out through participatory observation, in-depth interviews, and documentation. Data analysis was conducted using an interactive model that includes data reduction, data presentation, and conclusion drawing. The results of the study indicate that students learning culture is formed through four main themes: discipline, learning habits, social interaction, and learning motivation. Studfents learning culture has generally developed well, especially in aspects of discipline and social interaction, but is not evenly distributed in the aspect of independent learning habits. The role of teachers has proven to be a key factor in shaping a learning culture through learning strategies and classroom management, while the social and cultural environment also influences students learning habits. The findings of this study indicate that learning culture is the result of an on going interaction between internal and external factors of students. Systematic efforts are needed through culture-based learning, strengthening the role of teachers, and environmental support to improve students learning culture

Learning Culture of Elementary School Students: An Ethnographic Study at MI Nurul Huda Bengkulu City Fitri, Nabilah Aisyah; Hasanah, Annisa Nur; Friantary, Henny; Ahmad, Pahrozi; Yulizah, Yosi
International Journal on Advanced Science, Education, and Religion Vol 8 No 1 (2025): IJoASER (International Journal on Advanced Science, Education)
Publisher : Sekolah Tinggi Agama Islam Al-Furqan, Makassar - Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33648/ijoaser.v8i1.1605

Learning culture is an important factor in determining the success of the learning process in elementary schools. This study aims to describe students learning culture and analyze the factors that influence it at MI Nurul Huda, Bengkulu City. This study used a qualitative approach with an ethnographic design. Data collection techniques were carried out through participatory observation, in-depth interviews, and documentation. Data analysis was conducted using an interactive model that includes data reduction, data presentation, and conclusion drawing. The results of the study indicate that students learning culture is formed through four main themes: discipline, learning habits, social interaction, and learning motivation. Studfents learning culture has generally developed well, especially in aspects of discipline and social interaction, but is not evenly distributed in the aspect of independent learning habits. The role of teachers has proven to be a key factor in shaping a learning culture through learning strategies and classroom management, while the social and cultural environment also influences students learning habits. The findings of this study indicate that learning culture is the result of an on going interaction between internal and external factors of students. Systematic efforts are needed through culture-based learning, strengthening the role of teachers, and environmental support to improve students learning culture

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