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Journal : Bioscience

The Effect of Composting Azolla Compost Fertilizer and Humic Material on CO2 Gas Production in Sand Land Febrianti Rosalina; Muhammad Syahrul Kahar
Bioscience Vol 2, No 2 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (352.051 KB) | DOI: 10.24036/0201822100974-0-00

Abstract

his study was an experimental study which aimed to determine the effect of Compost Azolla Fertilizer (KA) and the addition of humic material (H) on the sand soil to the levels of CO2 produced in the respiration process of soil microorganisms (incubation soil conditions). The design used is a Completely Randomized Design (CRD) consisting of 9 treatments, namely 300 kg ha-1 urea (U), 4 tons ha-1 KA (T1), 15 liters ha-1 H (T2), 2 tons ha-1 KA + 15 liters ha-1 H (T3), 4 tons ha-1 KA + 15 liters ha-1 H (T4), 6 tons ha-1 KA + 15 liters ha-1 H (T5), 2 tons ha-1 KA + 30 liters ha-1 H (T6), 4 tons ha-1 KA + 30 liters ha-1 H (T7), 6 tons ha-1 KA + 30 liters ha-1 H (T8) and 1 control (K ) without the addition of train and H with 3 replications. Measurement of carbon dioxide (CO2) levels from each sample using gas chromatography equipped with TCD (Thermal Conductivity Detector). The statistical data analysis used was the F test, whereas to see the difference in the effect of each treatment, the BNT test was used. The results showed that Azolla compost fertilizer and humic material on sand soil had an effect on the level of CO2 gas production produced in the soil microorganisms respiration process, where the concentration of Azolla compost fertilizer and humic material which most affected the increase of CO2 gas production in sand soil was treated with the highest concentration of 6 tons ha-1 Azolla + 30 liter compost fertilizer ha-1 H (T8).
Soil Fertility Analysis with Soil Microorganism Indicators Febrianti Rosalina; sukmawati sukmawati
Bioscience Vol 6, No 2 (2022): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202262116811-0-00

Abstract

Areca nut and sago are typical fruits of the Papuan Community which produce waste. Utilization of waste for the community itself has not been done optimally, especially in agriculture. One way to overcome the problem of soil fertility is by adding organic matter. Areca nut shell waste and sago stem waste are alternative uses of existing waste by processing it into liquid organic fertilizer (POC) and biochar. Adding organic matter to the soil can increase soil productivity and fertility and can affect soil biological properties by increasing the activity of soil biota which play a role in breaking down and decomposing organic matter so that soil biological properties increase. The purpose of this study was to compare the biological properties of the soil applied with biochar and liquid organic fertilizer (POC) derived from areca nut shell and sago bark waste. The research method consisted of a completely randomized design (CRD) which consisted of 7 treatment levels. The first treatment (SB0) was control (without adding biochar), the second treatment (SB1) was areca nut shell waste biochar, the third treatment (SB2) was sago stem waste biochar, the fourth treatment (SB3) was a combination of areca nut shell and stem biochar. sago, the fifth treatment (SB4) was POC of areca nut shell waste, the sixth treatment (SB5) was POC of sago stem waste, and the seventh treatment (SB6) was a combination of POC of areca nut shell waste and sago stems. The parameters of this study included determining the total bacterial population, total fungal population, soil respiration, organic matter activity, nitrogen fixing activity, pH, Organic-C, and Total-N. Based on the results of the research that has been done, it can be concluded that the treatment in the form of biochar and POC in general has a significant effect on increasing soil pH, Organic-C, Total-N, total bacterial population, total fungi population, soil respiration, Nitrogen fixing activity, and The activity of organic matter. Treatment with biochar (SB1, SB2, and SB3) gave the highest average microbial population which was directly proportional to the respiration rate.Pinang dan sagu merupakan buah khas masyarakat Papua yang menghasilkan limbah. Pemanfaatan limbah bagi masyarakat itu sendiri belum dilkakukan secara optimal, terutama di bidang pertanian. Salah satu cara untuk mengatasi masalah kesuburan tanah adalah dengan pemberian bahan organik. Limbah kulit buah pinang dan limbah batang sagu menjadi alternatif pemanfaatan limbah yang ada dengan diolah menjadi pupuk organik cair (POC) dan biochar. Pemberian bahan organik ke dalam tanah dapat meningkatkan produktivitas dan kesuburan tanah serta dapat mempengaruhi sifat biologi tanah dalam meningkatkan aktivitas biota tanah yang berperan dalam merombak serta mendekomposisi bahan organik sehingga sifat biologi tanah meningkat. Tujuan dari penelitian ini adalah untuk membandingkan sifat biologi tanah yang diaplikasikan dengan biochar dan pupuk organic cair (POC) yang berasal dari limbah kulit buah pinang dan kulit batang sagu. Adapun metode penelitian ini terdiri dari Rancangan Acak Lengkap (RAL) yang terdiri dari 7 taraf perlakuan. Perlakuan pertama (SB0) adalah control (tanpa pemberian biochar), perlakuan kedua (SB1) adalah biochar limbah kulit buah pinang, Perlakuan ketiga (SB2) adalah Biochar limbah batang sagu, Perlakuan keempat (SB3) adalah kombinasi Biochar limbah kulit buah pinang dan batang sagu, perlakuan kelima (SB4) adalah POC limbah kulit buah pinang, Perlakuan keenam (SB5) adalah POC limbah batang sagu, dan Perlakuan ketujuh (SB6) adalah kombinasi POC limbah kulit buah pinang dan batang sagu. Parameter dari penelitian ini meliputi penetapan total populasi bakteri, total populasi fungi, respirasi tanah, Aktivitas perombak bahan organic, aktivitas penambat Nitrogen, pH, C-Organik, dan N-Total. Berdasarkan hasil penelitian yang telah dilakukan dapat disimpulkan bahwa pemberian perlakuan berupa biochar dan POC secara umum memberikan pengaruh yang nyata terhadap kenaikan pH tanah, C-Organik, N-Total, total populasi bakteri, total populasi fungi, respirasi tanah, aktivitas penambat Nitrogen, dan Aktivitas perombak bahan organic. Perlakuan dengan pemberian biochar (SB1, SB2, dan SB3) memberikan rata-rata populasi mikroba tertinggi yang berbanding lurus dengan laju respirasinya.
Potensi Bakteri Metanotrof sebagai Pereduksi Emisi Metan pada Lahan Pertanian Febrianti Rosalina; Sukmawati Sukmawati; Ponisri Ponisri; Anif Farida; Budi Satria; Ayu Diah Syafaati; Nuryanto Nuryanto
Bioscience Vol 7, No 1 (2023): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202371120929-0-00

Abstract

Konsentrasi Gas Rumah Kaca (GRK) meningkat seiring dengan aktivitas manusia dan menyebabkan peningkatan pemanasan global, salah satunya berasal dar sektor pertanian. Masih tingginya produksi emisi GRK pada sektor pertanian membutuhkan monitoring dan pengawasan secara berkala, sehingga dapat dipantau dan ditekan kuantitasnya. Untuk mengukur emisi GRK diperlukan inovasi dengan salah satu pengaplikasian bakteri metanotrof yang dapat menekan emisi di lahan pertanian. Tujuan dari penelitian ini adalah untuk mengetahui potensi bakteri metanotrof yang diaplikasikan pada lahan pertanian dalam mengurangi emisi metan. Perlakuan dalam penelitian terdiri dari 4 perlakuan diantaranya Sungkup 1 (Isolat bakteri MFb), Sungkup 2 (Isolat bakteri MFc), Sungkup 3 (Isolat bakteri MFd), dan Sungkup 4 (Isolat bakteri MFe). Pengambilan contoh gas dilakukan dengan metode sungkup tertutup (close chamber technique). Emisi metan (CH4) dianalisis secara langsung di lapangan dengan menggunakan alat digital berupa Alat Pintar Digital deteksi Kebocoran Gas Metana dan Propana AZ-7291 untuk mengukur CH4. Hasil penelitian menunjukkan bahwa terdapat pengaruh aplikasi bakteri metanotrof terhadap laju penurunan emisi gas metan. Dari semua perlakuan isolate bakteri metanotrof  yang diberikan, perlakuan bakteri metanotrof dengan kode MFe mampu menurunkan rata-rata emisi CH4 sebesar 305,449 mol/jam dan dianggap bahwa isolate tersebut adalah isolate yang paling baik diantara semua perlakuan.Greenhouse Gas (GHG) concentrations increase along with human activities and cause an increase in global warming, one of which comes from the agricultural sector. The high production of GHG emissions in the agricultural sector requires regular monitoring and supervision, so that the quantity can be monitored and suppressed. To measure GHG emissions, innovation is needed, one of which is the application of methanotrophic bacteria which can reduce emissions on agricultural land. The purpose of this study was to determine the potential of methanotrophic bacteria applied to agricultural land in reducing methane emissions. The treatment in this study consisted of 4 treatments including Chamber 1 (bacterial MFb isolate), Chamber 2 (bacterial MFc isolate), Chamber 3 (bacterial MFd isolate), and Chamber 4 (bacterial MFe isolate). Gas sampling was carried out using the closed chamber technique. Methane (CH4) emissions are analyzed directly in the field using a digital device in the form of a Methane and Propane AZ-7291 Digital Smart Leak Detection Tool to measure CH4. The results showed that there was an effect of the application of methanotrophic bacteria on the rate of reduction of methane gas emissions. Of all the isolates of methanotrophic bacteria given, the treatment of methanotrophic bacteria with the code MFe was able to reduce the average CH4 emission by 305.449 mol/hour and it was considered that the isolate was the best isolate among all the treatments.Keywords: Methanotrophic bacteria, GHG, methane, agricultural land