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All Journal Journal of Dentistry Indonesia Scientific Dental Journal Journal of Indonesian Dental Association Jurnal AKAL: Abdimas dan Kearifan Lokal Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal)
Widyarman, Armelia Sari
Faculty of Dentistry, Trisakti University
Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Dentistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Public Health Social Sciences Transportation

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Journal : Journal of Dentistry Indonesia

 1 
Persistent Endodontics Pathogens Biofilm Inhibited by Lactobacillus reuteri Indonesian Strain Widyarman, Armelia Sari; Lazaroni, Njoo Kiky Elysia
Journal of Dentistry Indonesia Vol. 26, No. 3
Publisher : UI Scholars Hub

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Lactobacillus reuteri is known as probiotics that effective to improve oral health. Objective: To analyze the effect of L. reuteri Indonesian strain, towards Enterococcus faecalis and Candida albicans biofilm growth. Methods: This study was conducted using biofilm assay. Each E. faecalis ATCC-29212 and C. albicans ATCC-10231 were cultured in Brain Heart Infusion Broth, distributed into 96 well-microplate and incubated for 24h, 37°C, anaerobic condition for E. faecalis and aerobic condition for C. albicans. L. reuteri LC382415 (Indonesian strain) was cultured in deMann Rogosa Shar pe Broth and diluted into different concentrations (108,106, and 104CFU/mL) subsequently, each concent ration dist ributed into biofilm well. Biofilm wells without probiotic was used as negative cont rol. Biofilm mass were measured using crystal-violet dye at 490nm using microplate-spectrophotometer. Data was statistically analyzed by one-way ANOVA test, statistical significance set as p<0.05. Results: Significant reduction of biofilm growth of E. faecalis and C. albicans after treatment with L. reuteri. The ideal concentration was found at L. reuteri 108 CFU/mL with 79.2% E. faecalis biofilm reduction and L. reuteri 104CFU/mL with 62.5% C. albicans biofilm reduction compared to control ( p<0.05) set at 100%. The ANOVA test results showed that L. reuteri in all concentrations and all time periods in this study had the ability to inhibit biofilm growth of both species ( p<0.05) compared to negative control. Conclusion: L. reuteri Indonesian strain inhibit the biofilm growth of E. faecalis and C. albicans. This antibiofilm effect may be useful in preventing biofilm growth in root canal.
Effectiveness of Lentinus edodes Mushroom Extract on Eradication of Enterococcus faecalis Biofilm Anggiarini, Putri Lestari; Amin, Meiny F; Gunawan, Juanita A; Widyarman, Armelia Sari
Journal of Dentistry Indonesia Vol. 27, No. 2
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Sodium hypochlorite is a commonly used irrigation solution in endodontic procedures, but it irritates tissues and has toxic effects. Lentinus edodes is a mushroom that has antibacterial properties. Enterococcus faecalis is an anaerobic bacterium that can cause root canal treatment failure. Objective: This study aimed to determine the effect of L. edodes extract on the eradication of E. faecalis biofilms. Methods: Phytochemical tests of L. edodes were performed to analyze alkaloids, steroids, triterpenoids, phenolics, tannins, flavonoids, and glycosides from this extract qualitatively. E. faecalis ATCC 29212 was cultured in brain heart infusion broth for 24 h at 37°C in an anaerobic atmosphere. Biofilm assay was performed to analyze the eradication of E. faecalis biofilm after treatment with L. edodes extract. The application times were 5, 15, and 30, and 10%, 20%, 40%, and 80% concentrations were used. Distilled water was used as a negative control, and NaOCl was used as a positive control. Data were statistically analyzed via one-way analysis of variance, where p < 0.05 was set as the level of significance. Results: L. edodes mushroom extract was effective in eradicating E. faecalis biofilms in all concentrations and incubation times compared with the control (p < 0.05). Significant differences were found between the application times of 5 and 15 min compared with 30 min (p < 0.05). The most effective concentration in eradicating E faecalis biofilms was 40% with an application time of 30 min. Conclusion: L. edodes mushroom extract proves its antibiofilm activity against E. faecalis biofilm. Further study is necessary to determine which substances are have the most influence on the effectiveness of L. edodes extract in eradicating E. faecalis biofilm in vivo.
Antibacterial and Antibiofilm Efficacy of Pineapple Hump (Ananas comosus) on Porphyromonas gingivalis in vitro Soulissa, Abdul Gani; Lombardo, Billy; Widyarman, Armelia Sari
Journal of Dentistry Indonesia Vol. 28, No. 3
Publisher : UI Scholars Hub

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Abstract

Background: Periodontal disease is one of the most prevalent oral health problems in Indonesia that affects supporting tissues of the teeth. Porphyromonas gingivalis plays an important role in the pathogenesis of periodontal disease. Alternative therapy with natural plant extracts, including pineapple (Ananas comosus) hump extract may inhibit the growth of bacteria that cause periodontal disease. Objective: To determine the effect of pineapple hump extract on bacterial growth and adhesion of Porphyromonas gingivalis biofilms. Method: The bacterial inhibition test was performed by the agar well diffusion method, and biofilm density measurements were made using the biofilm assay method. Results: Pineapple hump extract can inhibit bacterial growth optimally at a concentration of 100%, with an average zone of inhibition of 7.3 mm. The extract at a 50% concentration can eradicate the biofilms in a 6 h incubation time with an average OD of 0.124. The Shapiro-Wilk method confirmed a normal distribution of the data. Both one-way ANOVA and post hoc test showed a significant difference between the inhibitory ability of pineapple hump extract and between concentrations to restrict formation of Porphyromonas gingivalis biofilms. Conclusion: Pineapple hump extract was able to inhibit bacterial growth and eradicate the adhesion of Porphyromonas gingivalis biofilms in vitro.
The Effect of Parabiotic Reuterin on the Expression of Genes Involved in Candida albicans Biofilm Formation: An Ex vivo Study Riad, Anastasya Muna; Widyarman, Armelia Sari
Journal of Dentistry Indonesia Vol. 28, No. 3
Publisher : UI Scholars Hub

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Abstract

Candida albicans has a number of properties, including resistance to various antimicrobial agents, which allow it to survive in the root canals. Lactobacillus reuteri plays a role in maintaining oral health through interactions with the oral microbiome. L. reuteri has potential as a preventive and therapeutic agent against inflammatory diseases. Objective: The aim of this study was to investigate the effect of irrigation with a reuterin-containing solution on BCR1, ACE2, EFG1, and TEC1 gene expression in C. albicans root canal biofilms. Methods: L. reuteri was cultured in MRS broth and incubated anaerobically for 24 hours at 37°C. C. albicans was cultured in Sabouraud dextrose broth at 37°C for 48 hours. A total of 24 single-rooted premolar teeth were standardized and inoculated with C. albicans before irrigation with 50 μg/mL reuterin as a single, independent variable (Indonesian strain), 50 μg/mL reuterin Prodentis (a strain combination of L. reuteri DSM 17938 and L. reuteri ATCC PTA 5289), 2.5% sodium hypochlorite as positive control, and saline as negative control. A real-time quantitative polymerase chain reaction (RT-qPCR) assay was used to detect the expression of BCR1, ACE2, EFG1, and TEC1 in C. albicans root canal biofilms. Results: Reuterin significantly reduced the expression of BCR1 and ACE2 genes, which play a role in C. albicans biofilm formation, at the biofilm maturation stage (P < 0.05). Reuterin also affected the expression of the EFG1 and TEC1 genes, although the effect was not significant. Conclusion: A reuterin isolate of L. reuteri exhibits antibiofilm activity against the expression of C. albicans genes involved in biofilm formation. Reuterin has potential as an irrigation agent in the treatment of root canals. Further research is needed to shed light on the effectiveness of reuterin against the expression of genes that play important roles in the formation of C. albicans biofilms.
Co-Authors Adzra Fadla Taqia Amin, Meiny F Anastasia E Prahasti Anggiarini, Putri Lestari Arianne Dwimega Boy M Bachtiar Celia Radita, Diajeng Ciptadhi Tri Oka Binartha Dewi Liliany Margaretta, Dewi Liliany Dhara Nandary Drestia, Aradhea Monica Endang Winiati Bachtiar Erik Idrus Erni Erfan, Erni Faadiyah Nisa Febri Fahmawati Gunawan, Juanita A Hepziba, Evangelista Rachel Howis Josephine Janti Sudiono Jemima Pramadita Joko Kusnoto Josephine, Howis Kevin Lim Lazaroni, Njoo Kiky Elysia Lombardo, Billy Maulina, Syarifah Aulia Melanie Sadono Djamil Nely Soviati Nicolas Brian Simanjuntak Nurrahman, Hana Fauziyah Olivia Puspita Suhalim Putriany Agustin, Tri Riad, Anastasya Muna Richi, Mario Roeslan, Moehamad Orliando Sasqia Faadilah Andikoputri Shirley Trisna Yunita Soesanto, Sheila Soulissa, Abdul Gani Stella Pranoto Stephani Sumadi Stephanie Brigitta Widjaja Suwartini, Tien Theodorea, Citra Fragrantia Tjokro Prasetyadi Tri Erri Astoeti Tri Erri Astoeti Uppalavanna Witedja Widijanto Sudhana Widijanto Sudhana Yohana Yusra