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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Planta Tropika JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Asian Journal of Agriculture Berkala Penelitian Agronomi Tropical Wetland Journal Jurnal Proteksi Tanaman Tropika Rawa Sains: Jurnal Sains STIPER Amuntai Agroekotek View J-PEN Borneo : Jurnal Ilmu Pertanian Jurnal Pengabdian Kepada Masyarakat Jurnal Pengabdian Ilung (Inovasi Lahan Basah Unggul) Jalujur: Jurnal Pengabdian Masyarakat Jurnal Pengabdian Masyarakat ISEI jurnal proteksi tanaman tropika Alamtana
Muhammad Indar Pramudi
Department Of Plant Protection, Faculty Of Agriculture, Lambung Mangkurat University
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Classification of Fruit Flies In South Kalimantan Based on Morphology and Molecular Characters Pramudi, Muhammad Indar; Puspitarini, Retno Dyah; Rahardjo, Bambang Tri
Journal of Tropical Life Science Vol 3, No 3 (2013)
Publisher : Journal of Tropical Life Science

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Abstract

The result of identification from assemblies trapped by methyl eugenol and collection of fruit affected fruit flies at all location, there are seven specieses of fruitfly in all locations. Based on morphological identification, which analyzed UPGMA by using the CLAD 97 program, shows that the seventh species remain one group that consists of 5 sub groups. On the other hand, according to RAPD result, which analyzed UPGMA based on 20 DNA band characters, shows that the seventh species divided into two groups. The first group consists of B. umbrosa Fabricius, B. occipitalisis Bezzi, B. latifrons Hendell sub groups. The second group consists of Bactrocera carambolae Drew and Hancock, B. papayae Drew and Hancock, and B. albistrigata de Meijere, B. cucurbitae Coquillet sub groups. Sequencing result shows that homology of seventh fruitfly species, is 83 base pairs/bp (C), 101 bp (T), 265 bp (G), 420 bp (A), 432 bp (T), 600 bp (A) (appendixes 7). The length base pair for B. occipitalis, B. cucurbitae, B. albistrigata, B. carambolae, B. papayae, B. latifrons row is 615, 898, 570, 969, and 615 bp. An analysis result of morphology feature and RAPD method show distinction in group division and sub group, but the identification morphological and DNA of the seventh species fruitfly which is found are the similar.Keywords: Fruitfly, UPGMA, Morphoogy, RAPD, DNA
Classification of Fruit Flies In South Kalimantan Based on Morphology and Molecular Characters Muhammad Indar Pramudi; Retno Dyah Puspitarini; Bambang Tri Rahardjo
Journal of Tropical Life Science Vol. 3 No. 3 (2013)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The result of identification from assemblies trapped by methyl eugenol and collection of fruit affected fruit flies at all location, there are seven specieses of fruitfly in all locations. Based on morphological identification, which analyzed UPGMA by using the CLAD 97 program, shows that the seventh species remain one group that consists of 5 sub groups. On the other hand, according to RAPD result, which analyzed UPGMA based on 20 DNA band characters, shows that the seventh species divided into two groups. The first group consists of B. umbrosa Fabricius, B. occipitalisis Bezzi, B. latifrons Hendell sub groups. The second group consists of Bactrocera carambolae Drew and Hancock, B. papayae Drew and Hancock, and B. albistrigata de Meijere, B. cucurbitae Coquillet sub groups. Sequencing result shows that homology of seventh fruitfly species, is 83 base pairs/bp (C), 101 bp (T), 265 bp (G), 420 bp (A), 432 bp (T), 600 bp (A) (appendixes 7). The length base pair for B. occipitalis, B. cucurbitae, B. albistrigata, B. carambolae, B. papayae, B. latifrons row is 615, 898, 570, 969, and 615 bp. An analysis result of morphology feature and RAPD method show distinction in group division and sub group, but the identification morphological and DNA of the seventh species fruitfly which is found are the similar.Keywords: Fruitfly, UPGMA, Morphoogy, RAPD, DNA
Co-Authors Ahmad Kurnain Ahmad Syarifudin Anak Agung Istri Sri Wiadnyani Arwidya Winarni As’ari As’ari Bambang Tri Rahardjo Bambang Tri Rahardjo Baserah Baserah Bersinar, Saras Devaliana Catria Fikasari Dewi Fitriyanti Dewi Fitriyanti, Dewi Eka Febrianti Eza Galang Ramadhani Fendi Andiko Fikasari, Devaliana catria Firdaus, Ruly Hajijah Hajijah Hamidah . Harmi Harmi Hartini Hartini Helda Orbani Rosa Helda Orbani Rossa Hidayah, Lyly Ismed Fachruzi Ismed Setya Budi Jumar Jumar Kurnia Komala Sari Kurnia Komala Sari Liestiany, Elly Lyswiana Aphrodyanti Magfirah, Asmaul Mahmudah Mamudah Mariana Mariana Mariana Mariana Mariana Mariana Mariana Mariana Mariatul Qibtiyah Marisa Thaibah Marsuni, Yusriadi Maulana, Ikhsan Muhaimin . Muhammad Randy Muhammad Redho MUHAMMAD RIZAL Muhammad Yunidra Rahman Nisa Kamilah NOOR AIDAWATI Noorjannah Noorjannah Novita Agustina Nurdi, Mi’rajzul Hudia Oktaviani, Yerenia Teresa putra, Gusti Muhammad Ahsin Anggarda Rahma Rahma Rahma Rahma Ramadhani, Eza Galang Retno Dyah Puspitarini Ria Putri Rahmayani Rifandi, M Agung Rifani, Muhammad Indar Rima, Priska Deyana Rina Lestari Rinita Wulandari Risky Yanti S, Nur Khalifah SAFITRI, YUNIEKA AULIA Saipul Abbas Salamiah Salamiah . Salamiah . Salamiah ., Salamiah Salamiah Salamiah Salamiah Salamiah Salasiah . Samharinto Samharinto Separnawa, Muhammad Nur Sepe, Muslimin Siti Nur Azizah Sitti Nuur Jannah Sri Rahmawati Sri Rahmawati Surya Abdi susiana, Lilis Syahri Rajab Al Rahmat Tarigan, Divayona Begidir Br Tarigan Umie Shorayah Vera Trichoderma Winarni, Arwidya Yerenia Teresa Oktaviani Yulike Alivianiangsih Yunita Ambar Wati Yusriadi . Yusriadi Marsuni Yusriadi Yusriadi