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All Journal International Journal of Community Service Learning JURNAL KAJIAN VETERINER Jurnal Veteriner Nusantara
Simarmata, Yohanes T. R. M. R.
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GAMBARAN PATOLOGI ANATOMI PADA BABI LANDRACE SUSPECT AFRICAN SWINE FEVER (ASF) DI KABUPATEN KUPANG Simarmata, Yohanes T. R. M. R.; Tophianong, Tarsisius Considus; Amalo, Filphin Adolfin; Nitbani, Henny; Lenda, Victor
JURNAL KAJIAN VETERINER Vol 8 No 2 (2020): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v8i2.3074

Abstract

African Swine Fever (ASF) is a viral disease that attacks pigs and to date has caused many pig deaths in Kupang Regency. ASF is caused by a double-stranded DNA virus from the Asfivirus genus and the Asfarviridae family. This research aims to determine the anatomical pathology of the swine landrace suspect ASF. Organ samples were collected from two male landrace pigs and two female landrace pigs, aged 7 months, from Oeltuah Village, Taebenu District and Tarus Village, Central Kupang District, Kupang Regency, NTT. Clinical examinations were carried out on sick animals that were found during the investigation, then necropsied on the dead animals were carried out and continued with anatomical pathology examinations at the Pathology Laboratory, Faculty of Veterinary Medicine, Nusa Cendana University. Anatomical pathology examinations are carried out by observing changes in the structure and appearance of the organs. The necropsy results showed sub-cutaneous ecchymosis hemorrhage in the abdomen, limbs and ears, gastric, intestinal and hepatic hemorrhage, hemorrhagic lymphadenitis in mesenteric lymph nodes, hyperemic splenomegaly, pteckie hemorrhage in the renal capsule,, multifocal hemorrhage in the renal medulla and pulmonary lobe. Based on the observation of clinical symptoms and changes in anatomical pathology, it can be concluded that the death of pigs was suspected to be caused by the suspect ASF.
STUDI KASUS : FIBROSARCOMA PADA ANJING POMERANIAN MIX Simarmata, Yohanes T. R. M. R.; Biru, Desi M. A.; Restiati, Ni Made
JURNAL KAJIAN VETERINER Vol 9 No 1 (2021): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v9i1.3897

Abstract

Fibrosarcoma is a malignant neoplasm of fibroblast that commonly found in middle-aged or older dogs. Breed and sex do not influence the incidence of fibrosarcoma. A 10 years old grey female mix Pomeranian with a bodyweight of 9.2 kg showed a semi-solid mass, with meat-like consistency that felt integrated with the tissue underneath. A mass with a diameter of ± 5 cm had been found in the left thigh near the anus. The other two unusual masses with smaller size (diameter ±1 cm) were also found at lateral sinister near extremities cranial of the body. A serial of diagnostic check-ups, such as physical and clinical check-up, USG, haematology, cytology, and histopathology tests was run to examine the dog condition. The USG result showed hypoechoic masses with slightly anechoic appearances. The haematology analysis showed a decrease in lymphocyte, MCV and hematocrit, meanwhile the granulocyte, MCHC and MHC showed an increasing trend. The cytology test revealed a fat-like vacuolization. Furthermore, the histology examination indicating the presence of large hyperchromic oval cells. The histopathology examination also found fibroblast cells that suspected as tumour cells with mitotic and infiltrated oval cores. Thus, the dog was diagnosed with fibrosarcoma and the prognosis was dubious. The surgery was done to remove the tumour masses.
LAPORAN KASUS : PENANGANAN OBSTRUKSI USUS PADA ANJING DI BALI VETERINARY CLINIC Ludji Pau, Putri F.; Simarmata, Yohanes T. R. M. R.; Restiati, Ni Made
JURNAL KAJIAN VETERINER Vol 9 No 1 (2021): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v9i1.3899

Abstract

A local breed dog was brought to the veterinary clinic with signs of vomiting and decreased appetite. Physical examination revealed a pale mucosa of the eye and nose, 2 seconds of CRT, abdomen distention and stiffness. A haematology examination indicated WBC drop into 4.9, lymphocyte into 5.4, MCV to into 55.42 and Plate Distribusi Wide 8.1. The X-ray test showed the presence of an unknown mass around the intestine. Therefore, the dog was then diagnosed with intestinal obstruction. A laparotomy exploration with an enterotomy technique was performed to treat the condition. The surgery procedure found a mass of stone-like faeces that also consist of a mix of grass. The stone-like faeces were then removed from the intestine. Post-surgery monitoring was performed routinely and the dog received Cefotaxime Sodium (Claforan®) 22 mg/kg, Odansentron HCl (Zofran®) 0.2 mg/kg, Sucralfate (Carafate®) 0.8 gram, Lactulose (Cephulac®) 25 mL, Dexamethasone (Dexasone®) 1 mg/kg, Diphenhydramine HCl (Benadryl®) 3mg/kg, along with antiseptic treatment for the surgical incision. 3 days after the operation the dog began to show signs of recovery where the dog appeared to start moving and showed the desire to eat food. This condition can be prevented by not giving the dog food that bones. Lastly, the dog should be only released in a safe and knowledgeable environment.
Histopatologi Limpa dan Limfonodus pada Kasus Lapangan dengan Dugaan Kematian Akibat Virus African Swine Fever Pada Babi di Kabupaten Kupang Gelolodo, Maria Aega; Sanam, Maxs U. E.; Toha, Larry R. W.; Widi, Antin Y. N.; Simarmata, Yohanes T. R. M. R.; Murni, Theresia F. I. M. D.
JURNAL KAJIAN VETERINER Vol 9 No 2 (2021): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v9i2.4090

Abstract

African swine fever is a fatal hemorrhagic disease in the Suidae family that has become a significant economic challenge to the global pig farming industry. The continued spread of this disease has threatened global pork production and food security. Recognizing the disease manifestations and pathological changes of ASF is critical for a comprehensive and accurate early warning program. Knowledge of the key characteristics of this disease, such as its pathology anatomy, and histopathology, is also needed for early identification of ASF before establishing a tentative diagnosis. This article aims to discuss the pathologic changes and to update disease understanding in order to improve early detection of ASF in the field. A histopathological study of clinical samples collected during the February to April 2021 outbreak of ASF was performed to determine the characteristic lesions of ASF. Three dead ASFV-suspected pigs from a farm in Kupang regency were examined in this study. The main characteristics at the gross pathology inspection were hemorrhage and enlargement of the spleens and lymph nodes. The histopathologic findings confirmed spleen and lymph nodes hemorrhages, as well as congestion of spleen and follicle necrotic at the lymph nodes. Based on the clinical manifestation, pathological findings, and epidemiology observation, it is suspected that the pigs were infected with ASF. However, a molecular diagnostic test should be taken to confirm the definitive cause of the pig’s deaths.
GROSS PATHOLOGICAL FINDINGS OF AFRICAN SWINE FEVER SUSPECTS IN OEBELO, KUPANG REGENCY, 2021 Sanam, Maxs U. E.; Gelolodo, Maria Aega; Toha, Larry R. W.; Utami, Tri; Simarmata, Yohanes T. R. M. R.; Murni, Theresia F. I. M. D.
JURNAL KAJIAN VETERINER Vol 9 No 3 (2021): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v9i3.7869

Abstract

African swine fever (ASF) is a destructive re-emerging swine disease that has posed a serious economic threat to the global pig farming sector. In past years, ASF has rapidly spread over Europe, Asia, and Oceania, and begin to enter Indonesia in the middle of 2019. The clinical and pathological symptoms of ASF are influenced by the strain's virulence, the transmission pathway, and the pig's immunological and health status. ASF’s clinical manifestations are known to evolve, from after an invasion enters a new free region to after the disease has been established in the territory for a longer period. Identifying ASF clinical signs and pathological changes is crucial for a comprehensive and reliable early detection system. The objective of this research is to observe and identify gross pathology in ASF suspect pigs in order to obtain a better understanding of the cause of death. Two dead pigs from a farm in Oebelo village, Kupang regency, Indonesia with a recent history of massive deaths had been examined in this study. The post-mortem results showed that hemorrhagic splenomegaly and hemorrhagic lymphadenitis were the main lesions observed at the examinations. Furthermore, hemorrhages were also found in various internal organs such as the kidneys, liver, and heart. To determine the exact cause of the pigs' deaths, a molecular diagnostic test should be conducted.
Gambaran Hematologi Darah Pasca Vaksinasi Porcine Reproductive and Respiratory Syndrome (PRRS) pada Ternak Babi di Kabupaten Kupang Sole, Marsyella Gloria; Simarmata, Yohanes T. R. M. R.; Gelolodo, Maria Aega
JURNAL KAJIAN VETERINER Vol 12 No 1 (2024): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v12i1.15129

Abstract

The potential for expanding pig farming, particularly with native pigs, in East Nusa Tenggara (NTT) Province is significant due to the predominant non-Muslim population in NTT who utilise pigs in traditional and religious ceremonies. However, the high number of pigs in Kupang Regency and conventional farming practices can elevate the likelihood of infections like Porcine Reproductive and Respiratory Syndrome, known as PRRS. Porcine reproductive and respiratory syndrome (PRRS), caused by the PRRS virus (PRRSV), is a significant disease in the pig farming sector. The porcine reproductive and respiratory syndrome virus is highly contagious within pig herds. It can spread through direct contact or contaminated aerosols. Vaccines have been used to limit the disease’s transmission, despite often being considered ineffective. This study analyses the variations in blood haematological parameters before and after PRRS immunisation, including RBC, HGB, PCV, MCV, MCH, MCHC, and WBC. Fifteen pigs were included in the sample. Sampling was conducted two times from the same pigs: before PRRS vaccination and one month post-immunization. SPSS was used for the data analysis. A statistical study using SPSS showed no significant difference in blood hematology following the PRRS vaccination.
Diagnosa Penyakit Hewan dengan Teknik Polymerase Chain Reaction di Lingkungan Universitas Nusa Cendana Gelolodo, Maria Aega; Pandarangga, Putri; Simarmata, Yohanes T. R. M. R.; Utami, Tri; Tophianong, Tarsisius Considus; Datta, Frans Umbu; Sitompul, Yeremia Y.; Gaina, Cynthia Dewi; Foeh, Nancy Diana F. K.; Deta, Herlina Umbu
International Journal of Community Service Learning Vol. 7 No. 2 (2023): May 2023
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/ijcsl.v7i2.55140

Abstract

Penyakit infeksius dapat menyebabkan kerugian ekonomi dan dampak sosial budaya di masyarakat. Diagnosa dan deteksi dini terhadap agen patogen penyebab penyakit infeksius merupakan langkah penting dalam mencegah efek yang lebih besar dari penyakit infeksius. Semakin cepat suatu agen penyakit terdeteksi maka makin cepat pula usaha pencegahan maupun kontrol yang dilakukan untuk mencegah penyebaran lebih lanjut dari penyakit tersebut. Salah satu teknik deteksi laboratorik yang paling banyak digunakan saat ini adalah teknik molekuler PCR. Teknik ini memiliki sensitivitas dan spesifitas yang tinggi dan dapat dilaksanakan dalam waktu yang relatif singkat. Terlepas dari keunggulan serta keuntungan teknik PCR ini, namun pada kenyataannya masih ada stakeholders di tingkat universitas yang belum sepenuhnya mengenal maupun memahami dengan baik teknik ini. Oleh karena itulah maka pelatihan PCR pada para dosen maupun tenaga laboran di lingkungan Universitas Nusa Cendana, Kupang menjadi penting dilakukan dalam rangka peningkatan kapasitas deteksi laboratorik terhadap penyakit infeksius. Kegiatan yang diikuti oleh 14 orang peserta ini selain melengkapi para peserta dengan pengetahuan mengenai prinsip dasar PCR dan aplikasinya juga melatih kemampuan peserta dalam melaksanakan PCR di laboratorium.
Gambaran Hematologi 3 Bulan Pasca Vaksinisasi Porcine Reproductive and Respiratory Syndrome (PRRS) pada Ternak Babi di Kabupaten Kupang Simarmata, Yohanes T. R. M. R.; Gelolodo, Maria A.; Sitompul, Yeremia Y.; Sole, Marsyella G.
JURNAL KAJIAN VETERINER Vol 12 No 2 (2024): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v12i2.15132

Abstract

Pigs are the most commonly raised livestock in Kupang Regency, offering various advantages over other animals. However, the growing pig population poses a higher risk of diseases such PRRS, which affects both respiratory and reproductive health. Vaccination remains a crucial method for preventing PRRS. Post-vaccination haematological examinations are essential for assessing immune responses, utilizing parameters like leukocyte and lymphocyte counts to evaluate vaccine efficacy and safety. This study investigates haematological parameters as indicators of physiological responses to PRRS vaccination, a relatively less explored area compared to other immunological assessments. The research analysed haematological parameters such as RBC, HGB, PCV, MCV, MCH, MCHC, and WBC both before and three months after vaccination. Blood samples were collected from 15 pigs one day prior to and three months following vaccination. SPSS software was used to analyse the data. The results indicated that haematological parameters remained within normal ranges. RBC, HGB, PCV, and MCHC did not significantly differ, however MCH, MCV, and WBC levels did indicate statistically significant variations. These results support the safety and efficacy of PRRS immunization by indicating that it causes detectable haematological alterations. This study underscores the importance of using haematological parameters as reliable indicators for assessing vaccine pigs reaction.
LAPORAN KASUS: FUNGAL RHINITIS PADA ANJING LOKAL (Canis familaris) Sau, Astrid Alviani; Sitompul, Yeremia Yobelano; Simarmata, Yohanes T. R. M. R.
Jurnal Veteriner Nusantara Vol 8 No 1 (2025): Februari, 2025
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v8i1.16440

Abstract

Kesehatan hewan peliharaan perlu diperhatikan karena hewan peliharaan hidup berdampingan dengan manusia dan berpotensi membawa penyakit yang zoonosis maupun non zoonosis. Penyakit pada anjing dapat berupa infeksi virus, jamur, bakteri maupun parasit. Infeksi jamur pada anjing dapat ditularkan melalui inhalasi, konsumsi atau melalui luka pada kulit. Terdapat banyak spesies jamur yang ada di lingkungan, dan sebagian besar infeksi adalah dari tanah. Pemeriksaan fisik menunjukkan anjing kasus memiliki skor kondisi tubuh yaitu 2/5. Terdapat kotoran mata, hidung dan mata kiri mengalami pembengkakan, epistaksis, rambut kusam, pada beberapa bagian tubuh mengalami alopesia dan lesi akibat jamur. Hasil pemeriksaan hematologi menunjukan adanya leukositosis dan kultur jamur menunjukkan adanya pertumbuhan jamur Aspergillus spp. Pemberian terapi suportif dilakukan untuk membantu mempertahankan kondisi umum tubuh anjing. Anjing kasus diberikan injeksi Biodin 0,5 ml secara IM dan Vitamin B complex 0,5 mL secara IM. Penanganan simptomatis diberikan Bromhexine HCL sebagai mukolitik dengan dosis anjuran 2 mg/kg PO dua kali sehari. Terapi kausatif diberikan Itraconazole dengan dosis anjuran 5 mg/kg PO satu kali sehari. Pasca terapi, perbaikan kondisi berupa perubahan tingkah laku menjadi lebih aktif dan peningkatan nafsu makan, bengkak pada bagian mata berkurang, leleran yang keluar dari hidung berkurang dan tidak lagi bercampur darah.
Molecular Identification of African Swine Fever Virus from an Outbreak in Kupang Region Gelolodo, Maria Aega; Sanam, Maxs U. E.; Tangkonda, Elisabet; Simarmata, Yohanes T. R. M. R.; Murni, Theresia F. I. M. D.; Jacob, Jois M.
JURNAL KAJIAN VETERINER Vol 13 No 1 (2025): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v13i1.19609

Abstract

African Swine Fever (ASF) adalah infeksi virus penting pada babi yang belakangan ini muncul kembali sebagai penyakit lintas batas yang menyebar dengan luas dan cepat. Konfirmasi pertama ASF tercatat di Indonesia pada akhir tahun 2019 dan kemudian meluas ke beberapa daerah termasuk Nusa Tenggara Timur (NTT). Informasi tentang virus ASF di NTT terbatas hingga saat ini. Studi ini bertujuan untuk mengidentifikasi keberadaan virus ASF pada kasus kematian massal babi di Kupang, Pulau Timor. Sampel ginjal dan limpa diambil dari delapan ekor babi yang mati mendadak di daerah Kupang. Teknik PCR konvensional dilakukan untuk mengidentifikasi daerah yang sangat terkonservasi dari genom virus ASF VP72. Hasil PCR menunjukkan bahwa semua sampel menampilkan panjang pita yang sesuai dengan pita target dari primer yang digunakan. Hasil ini menunjukkan bahwa virus ASF masih beredar di wilayah Kupang dan tetap menjadi ancaman bagi peternakan babi di daerah tersebut. Oleh karena itu, usaha untuk meningkatkan kesadaran publik terutama di kalangan produsen babi mengenai pentingnya penerapan biosekuriti harus dilakukan secara konsisten.
Co-Authors Biru, Desi M. A. Deta, Herlina Umbu Filphin Adolfin Amalo Foeh, Nancy Diana F. K. Gaina, Cynthia Dewi Jois M. Jacob, Jois M. Ludji Pau, Putri F. Maria Aega Gelolodo Maxs Urias Ebenheizer Sanam Murni, Theresia F. I. M. D. Nitbani, Henny Restiati, Ni Made Sau, Astrid Alviani Sitompul, Yeremia Y. Sitompul, Yeremia Yobelano Sole, Marsyella G. Sole, Marsyella Gloria Tangkonda, Elisabet Tarsisius Considus Tophianong, Tarsisius Considus Toha, Larry R. W. Tri Utami Victor Lenda Widi, Antin Y. N.