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IMPROVEMENT HATCHING AND QUALITY NAUPLI THROUGH THE PROVISION OF FRESH FEED DIFFERENT IN FEMALE PARENT SHRIMP VANAME ( LITOPENAEUS VANNAMEI ) A MANUSCRIPT PUBLICATION Wahyu Parmana Saputra; Riza Rahman Hakim; Hariyadi Hariyadi
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 6 No. 2 (2023): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v6i2.6053

Abstract

Fresh natural feeds such as marine worms, squid, and oysters are rich sources of amino acids, proteins, and essential fatty acids, including docosahexaenoic acid (DHA), arachidonic acid (AA), and eicosapentaenoic acid (EPA), which are important for shrimp broodstock reproduction and seed production. Marine worms, in particular, contain a high protein content of approximately 56.29%. This study aims to evaluate the effects of different fresh feed types on the hatching rate (HR) of eggs and the quality of nauplii produced by female broodstock of Pacific white shrimp, Litopenaeus vannamei. The study employed an experimental method using a Completely Randomized Design (CRD). Five feeding treatments were tested with three replicates each: marine worms, squid, oysters, a combination of marine worms and oysters, and commercial pellet feed as the control. Data were analyzed using Analysis of Variance (ANOVA), followed by the Least Significant Difference (LSD) test to determine significant differences among treatments. The results showed that different types of fresh feed significantly affected egg hatching rate. The highest hatching rate was obtained from broodstock fed marine worms, reaching 80%, which corresponded to approximately 124,630 successfully hatched larvae. Fresh feed type also had a highly significant effect on nauplius quality, as indicated by developmental stage progression at 38 hours after hatching. Broodstock fed marine worms produced nauplii that reached an average developmental stage of Nauplius VI, indicating superior larval quality compared with the other treatments. Water quality parameters during broodstock maintenance remained within acceptable ranges, with temperature ranging from 29–31°C, dissolved oxygen from 5.7 mg L⁻¹, pH at 8.0, and salinity at 31 ppt. In conclusion, the intensive use of fresh marine worms as broodstock feed can significantly improve egg hatching rate and enhance nauplius quality in Pacific white shrimp (L. vannamei).
EFFECT OF ADDITION OF GROWTH REGULATOR SUBSTANCE CONCENTRATION ON THE GROWTH Tetraselmis chuii CULTURE IN LABORATORY SCALE Diki Restu Setiawan; Ganjar Adhywirawan Sutarjo; Riza Rahman Hakim
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 6 No. 2 (2023): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v6i2.6543

Abstract

Tetraselmis chuii is one type of phytoplankton that can be used as a natural food source. According to FAO-UN (2016) Tetraselmis chuii has 83,400% protein content, 32,500% carbohydrate and 45,700% lipid. Higher than Chaetoceros gracili, Nannochloropsis oculata, Dunaliellatertiolecta and Isochrysis galbana. In cultured phytoplankton nutrient composition of the culture medium was very instrumental in the growth. Microalgae growth with culture can reach optimum by mixing sea water with nutrients that are not contained in the sea water. Related to this, various efforts have been made in an effort to increase the growth of natural feed, both cultivation methods and the addition of various growth substances. One substance that has the potential to increase the growth of natural feed is the use of growth hormones to increase the growth of natural feed, namely through population density, he application form is by adding growth regulators. This research was carried out at the Natural Feed Laboratory at the Center for Aquaculture of Sea Water Fisheries, Situbondo. This study used Completely Randomized Design (CRD) yanang cultured laboratory scale with 7 treatments and 4 repetitions. The treatment in this study was the administration of 2.4-D-Acid Growth Regulating Substances with different doses. The main parameters used include growth measurements by calculating the density and growth rates Tetraselmis chuii. Furthermore, the data were analyzed using ANOVA followed by LSD test. The addition concentration of plant growth regulator effect on cell density and growth rate Tetraselmis chuii culture laboratory scale. Provision of plant growth regulator with konssentrasi 5 ml + 1 ml Walne produce the highest density of 2.323125 cells/ml and a maximum growth rate that is 173 269 cells/ml/day.
Effect of Salinity Differences for Growth Chlorella sp. on the Laboratory Scale Ais Anantama Wibowo; Hariyadi Hariyadi; Riza Rahman Hakim
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 6 No. 2 (2023): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v6i2.6608

Abstract

Chlorella sp. growth is dependent on environmental conditions. One of environmental condition affect the growth of Chlorella sp. is salinity. Salinity can affect the growth of Chlorella sp. because salinity affects the osmosis pressure on the cell wall of Chlorella sp. in the absorption of nutrients in the culture media. This study aims to determine the effect of different salinity on the growth of Chlorella sp. This study was conducted on laboratory scale using experimental methods. This research used Walne fertilizer on culture media were given Chlorella sp. as many as 100,000 cells / ml in every treatment. This study uses 4 treatments and 3 repetitions. The treatment in this study with salinity differences, namely salinity 15 ppt, 20 ppt, 25 ppt and 30 ppt. Chlorella sp. growth data every day obtained from the calculation of the sample Chlorella sp. use haemocytometer. The calculation data obtained is then analyzed using anava one factor data. The results showed that differences in salinity had no effect on the growth of Chlorella sp. The best salinity for the growth of Chlorella sp. is at 15 ppt salinity with the number of Chlorella sp cells. at peak growth as much as 340 × 104 cells / ml, followed by treatment B salinity 20 ppt as much as 339.33 × 104 cells / ml, then treatment D salinity 30 ppt as much as 270.58 × 104 cells / ml, and treatment C 25 salinity as much as 266.83 × 104 cells / ml. Salinity of 15 ppt, 20 ppt, 25 ppt, and 30 ppt can be used for cultivation Chlorella sp.
THE EFFECT OF DIFFERENT VALUE CONCENTRATION ON SPIRULINA PLATENSIS TOWARD THE BRIGHTNESS OF COLORS ON GOLDFISH (CARASSIUS AURATUS) Sary Astuty Aceh; Riza Rahman Hakim; Hariyadi Hariyadi
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 6 No. 2 (2023): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v6i2.7313

Abstract

Body coloration is one of the most important quality attributes determining the ornamental value of goldfish (Carassius auratus). Since fish are unable to synthesize carotenoids, dietary supplementation with natural carotenoid sources is required to enhance pigmentation. Spirulina platensis is a microalga rich in carotenoids that has the potential to improve fish coloration. Therefore, this study aimed to evaluate the effect of different concentrations of Spirulina platensis meal in feed on the color enhancement of goldfish. The study employed an experimental method using a Completely Randomized Design (CRD) consisting of six treatments with three replications. The treatments included feed supplemented with 0% (control), 1%, 2%, 3%, 4%, and 5% Spirulina platensis meal. Fish were reared for four weeks, and color intensity of the head, body, fins, and tail was assessed periodically. Water quality parameters, including temperature, pH, and dissolved oxygen (DO), were also monitored throughout the experiment. Data were analyzed using analysis of variance (ANOVA) followed by Duncan’s multiple range test. The results showed that dietary supplementation with Spirulina platensis significantly affected body coloration but had no significant effect on the coloration of the head, fins, and tail. The highest body color intensity was observed in fish fed diets containing 3% Spirulina platensis, with an average color score of 56.55 ± 0.59, significantly higher than the control treatment. Water quality remained within suitable ranges for goldfish culture, with temperatures of 23–26°C, pH 7, and DO ranging from 6.7 to 9.9 mg/L. In conclusion, supplementation of 3% Spirulina platensis meal in feed effectively enhances the body coloration of goldfish and can be recommended as a natural pigment source in ornamental fish culture.
Co-Authors Agista Tsani Untsa Ahmad Jumarding Ahmad Rusyda Hafidhi Albasa Ais Anantama Wibowo Albasa, Ahmad Rusyda Hafidhi Alisatul Amalia Andriyanto Andriyanto Anis Zubaidah Anis Zubaidah Anis Zubaidah Arfida Boedirochminarni Ari Wahyu Darmawan Arinta Agnie Dewantari Artadana, Ida Bagus Made Astuti, Triana Indri Azhari Azhari Bahiyya, Choridatul Budi Setyono Choridatul Bahiyya Dahlia, Lisma Darmawan, Ari Wahyu David Hermawan David Hermawan David Hermawan Dewantari, Arinta Agnie Dianita Wijayanti Diki Restu Setiawan Ditya, Yoga Candra Dony Prasetyo Erni Indrawati Felix Miranda Fitri, Ruhul Jihadil Ganjar Adhywirawan Sutarjo Ganjar Adhywirawan Sutarjo Gema Wahyudewantoro, Gema Gitra - Yoga Hany Handajani Hariyadi Hariyadi Hariyadi Hariyadi Hariyadi, Hariyadi Hermawan, David Hidayat, Reyhan Rafisyah Indrawan, Rindya Fery Irzal Effendi Ismi, Yuliani Jamhari Jamhari Kholista, Marry Ann Lailiyah, Ummi Badriyatul Lili Zalizar Lisma Dahlia M. Tang Machmud Effendy Machmud Effendy Machmud Effendy Marry Ann Kholista Maulidiya, Nurfitri Mega Fatimah Rosana Meishela Dwi Lawidya Miranda, Felix Moh. Nurhuda Mustaqim Mustaqim, Mustaqim Novin Farid Styo Wibowo Nur Subeki Nurfitri Maulidiya Nurhuda, Moh. Olivia Agustin Olivia Agustin Puji Sumarsono Rasyidi, Emil Salim Ridwan Ridwan Rini Mastuti Rogerio Lainus Tilman Sarmento Sary Astuty Aceh Soni Andriawan Subairi Subairi Suryadewi, Nindya Suwandayani, Beti Istanti Triana Indri Astuti Untsa, Agista Tsani Utomo, Waskito Dwi Wahyu Parmana Saputra Warkoyo Warkoyo Waskito Dwi Utomo Wenny Novita Sari Worowirastri E., Dyah Yohana Agustina Yudhistyan Hanif Rahmawan Yuliani Ismi Yus Mochamad Cholily