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All Journal International Journal of Evaluation and Research in Education (IJERE) JURNAL ILMIAH PLATAX
Ginting, Elvy Like
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Isolation and Screening the Symbiont Bacteria of the Sponge Dragmacidon sp from Manado Bay, North Sulawesi that Producing Chitinase and Protease Sembiring, Sindiy Cloudya; Warouw, Veibe; Wullur, Stenly; Bara, Robert A; Salaki, Meiske S.; Ginting, Elvy Like
Jurnal Ilmiah Platax Vol. 9 No. 1 (2021): ISSUE JANUARY - JUNE 2021
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.9.1.2021.34307

Abstract

Enzymes are important in the technology industry and hydrolytic enzymes, such as chitinase and protease are commonly used for it. Various types of microorganisms such as bacteria can produce hydrolytic enzymes.  Sponge-associated bacteria are excellent sources of extracellular hydrolytic enzymes because the surface and internal spaces of sponges are richer in nutrients. The aim of this study was to isolate and screen the bacteria of the sponge Dragmacidon sp symbiotic from Manado Bay, North Sulawesi that producing chitinase and protease   Symbiont bacteria were grown in Zobell 1226 E medium with a dilution of 10-4. Bacterial isolation was carried out based on the morphological characteristics of the colony. Chitinase and protease activity was carried out by growing each bacterial isolate in chitin and protein media at 36oC for 48 hours. Chitinase and protease activities were indicated by the formation of a clear zone around the bacterial colony, however, the clear zone for chitinase activity was observed after pouring the Lugol's solution. Based on this study, 8 isolates bacteria of the symbiotic spongy Dragmacidon sp from Manado Bay, North Sulawesi were isolated based on morphological characteristics. The colony of the bacteria is generally white with an irregular shape. Four isolates, namely 1, 2, 3, and 8 had chitinase activity with chitinolytic indexes were 1.7; 1.5; 1.4, and 1.3, respectively. Six isolates, namely 1, 2, 3, 4, 5, and 6 had protease activity with proteolytic indexes were 1.4; 1.8; 3.1; 1.3; 1.8; and 2.5, respectively.Keywords: Bacteria; Chitinolytic; Proteolytic; Symbiont; SpongeAbstrakEnzim menempati posisi penting dalam bidang teknologi dan industri. Enzim yang banyak digunakan dalam bidang industri adalah enzim hidrolase. Enzim dapat diisolasi dari berbagai jenis mikroorganisme seperti bakteri. Bakteri yang berasosiasi dengan spons merupakan sumber enzim hidrolitik ekstraseluler yang sangat baik karena permukaan dan ruang internal spons lebih kaya nutrisi. Penelitian ini bertujuan untuk mengisolasi dan menguji aktivitas kitinase dan protease bakteri simbion spons Dragmacidon sp dari Teluk Manado. Bakteri simbion spons ditumbuhkan dalam media Zobell 1226 E pada pengenceran 10-4. Isolasi bakteri dilaksanakan berdasakan karakteristik morfologi. Aktivitas kitinase dan protease dilaksanakan dengan menumbuhkan setiap isolat bakteri dalam media kitin dan protein pada suhu 36oC selama 48 jam. Aktivitas kitinase dan protease ditandai dengan terbentuknya zona bening di sekitar koloni bakteri yang mana untuk kitinase diamati setelah diberi larutan lugol. Berdasarkan penelitian ini, 8 isolat bakeri simbion spon Dragmacidon sp dari Teluk Manado, Sulawesi Utara berhasil diisolasi berdasarkan karakteristik morfologi. Isolat bakteri umumnya berwarna putih dengan bentuk ireguller. Empat isolat yakni 1, 2, 3, dan 8 memiliki aktivitas kitinase dan enam isolat yakni 1, 2, 3, 4, 5, dan 6 yang memiliki aktivitas protease. Indeks kitinolitik dari masing-masing keempat isolat bakteri secara berturut turut adalah 1,7; 1,5; 1,4; dan 1,3 dengan kategori bernilai rendah dan indeks proteolitik adalah 1,4; 1,8; 3,1; 1,3; 1,8; dan 2,5 dengan kategori bernilai rendah sampai tinggi.Kata kunci: Bakteri; Kitinolitik; Proteolitik; Simbion; Spons
Isolation and Antibacterial Activity Test of Seagrass Epiphytic Symbiont Bacteria Thalassia hemprichii from Bahowo Waters, North Sulawesi Maarisit, Ismariani; Angkouw, Esther D.; Mangindaan, Remy E. P.; Rumampuk, Natalie D. C.; Manoppo, Henky; Ginting, Elvy Like
Jurnal Ilmiah Platax Vol. 9 No. 1 (2021): ISSUE JANUARY - JUNE 2021
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.9.1.2021.34320

Abstract

Seagrass is a higher plant and has the ability to produce bioactive compounds such as antibacterial. Seagrass is also a host to a variety of bacteria. Bacteria that live in the host will produce the same compounds as the host's body. The utilization of symbiotic bacteria with seagrasses as producers of bioactive compounds such as antibacterial can be used as a solution to reduce excessive seagrass uptake in nature. On the other hand, bacteria have the advantage of being fast and easy to grow and can be mass-produced and more economical. This study aims to isolate and test the antibacterial activity of the epiphytic bacteria of seagrass symbionts. Epiphytic bacteria of seagrass symbionts were grown on Nutrient Agar media directly in the field and bacterial isolation was carried out based on the morphological characteristics of the bacterial isolates. The antibacterial activity test was carried out using the disc method with the test bacteria Stapylococcus aureus, Streptococcus mutans, Escherichia coli, Salmonella thypi, and antibiotics as positive controls. The ability of bacteria to produce antibacterial was indicated by the formation of an inhibition zone around the paper disc containing the epiphytic bacteria of the seagrass symbiont T. hemprichii. A total of 3 isolates of epiphytic bacteria were isolated from T. hemprichii seagrass from Bahowo Waters, Tongkaina Village, Bunaken District, these isolates are namely Epifit 1, Epiphyte 2, and Epiphyte 3. Epiphyte 2 isolate had antibacterial activity against S. mutans, S. aureus, and S. thypi test bacteria, Epiphyte 3 isolate had antibacterial activity against S. mutans, and S. thypi test bacteria.Key words: Bacteria; Antibacterial; T. hemprichii; symbionts; BahowoAbstrakLamun merupakan tumbuhan tingkat tinggi dan memiliki kemampuan menghasilkan senyawa  bioaktif seperti antibakteri. Lamun juga merupakan tempat hidup atau inang dari berbagai bakteri. Bakteri yang hidup pada inang akan menghasilkan senyawa yang sama dengan tubuh inangnya. Pemanfaatan bakteri yang bersimbiosis dengan lamun sebagai produsen senyawa bioaktif seperti antibakteri dapat dijadikan sebagai solusi dalam mengurangi pengambilan lamun yang berlebihan di alam. Dilain pihak, bakteri memiliki keunggulan karena pertumbuhan bakteri yang cepat dan mudah tumbuh, dapat diproduksi secara massal dan lebih ekonomis. Penelitian ini bertujuan untuk mengisolasi dan menguji aktivitas antibakteri dari bakteri epifit simbion lamun T. hemprichii dari Perairan Bahowo.  Bakteri epifit simbion lamun ditumbuhkan pada media NA secara langsung di lapangan dan isolasi bakteri dilaksanakan berdasarkan karateristik morfologi isolat bakteri. Uji aktivitas bakteri dilakukan menggunakan metode cakram dengan bakteri uji S. aureus, S. mutans, E. coli, dan S. thypi dan antibiotik sebagai kontrol positif. Kemampuan bakteri menghasilkan antibakteri ditandai dengan terbentuknya zona hambat disekitar kertas cakram yang mengandung bakkteri epifit simbion lamun T. hemprichii.  Sebanyak 3 isolat bakteri epifit berhasil diisolasi pada lamun T. hemprichii dari Perairan Bahowo, Kelurahan Tongkaina, Kecamatan Bunaken yaitu Epifit 1, Epifit 2, dan Epifit 3. Isolat epifit 3 memiliki aktivitas antibakteri terhadap bakteri uji S. thypi, isolat Epifit 2 terhadap bakteri uji S. mutans, S. aureus, dan S. thypi, isolat Epifit 3 terhadap bakteri uji S. mutans, dan S. thypi.Kata kunci: Bakteri; Antibakteri; T. hemprichii; Simbion; Bahowo
Molecular Identification And Conservation Status Of Sharks From The Fins Trade In Manado City North Sulawesi Mopay, Maratade; Wullur, Stenly -; Onibala, Hens -; Ginting, Elvy Like; Rumengan, Inneke F. M; Sondak, Calvyn F.A; Sumilat, Deiske A.
Jurnal Ilmiah Platax Vol. 9 No. 2 (2021): ISSUE JULY-DECEMBER 2021
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v9i2.36016

Abstract

Sharks are a group of cartilaginous fish that are vulnerable to overfishing.  Genetics approaches play an important role in shark conservation. Shark fishing has become the main activity of fishermen in several areas, including in North Sulawesi. This research is focused on the molecular aspects and conservation status of shark species obtained from the shark fin trade in Manado, North Sulawesi. COI gene was amplified using Fish BCL5 (for) and HCO219 (rev) primers. Nucleotide sequences of each sample were aligned with the closest sequences in the GenBank database using the BLAST (Basic Local Alignment and Search Tool) method. The conservation status of the shark species is carried through the IUCN (International Union for the conservation of nature red list) Red list website. Molecular identification results showed that the shark’s fins from Manado had high similarity with Carcharhinus falciformis (HM1 and HM2) and Carcharhinus melanopterus (HM3). According to IUCN red list data, the C. falciformis and C. melanopterus were categorized as vulnerable to extinction (VU).Keywords:  Molekuler; COI; Shark; Manado and IUCN Red list . AbstrakHiu merupakan kelompok ikan bertulang rawan yang sangat rentan terhadap dampak penangkapan secara berlebihan.  Informasi terkait genetik hiu  yang semakin terancam populasinya sangat berperan penting dalam upaya konservasi hiu. Penangkapan hiu telah menjadi aktivitas utama nelayan di beberapa daerah, termasuk di Sulawesi Utara. Penelitian ini difokuskan pada aspek  molekuler dan penentuan status konservasi spesies hiu menggunakan sampel sirip yang di dapatkan  dari perdagangan sirip  hiu, di kota Manado.  Amplifikasi gen COI dilakukan dengan menggunakan primer Fish BCL5 (for) dan HCO219 (rev). Sekuens nukleotida masing-masing sampel disejajarkan dengan nukleotida terdekat yang ada dalam database genbank menggunakan metode BLAST (Basic Local Aligment and Search Tool)  Penentuan status konservasi dilakukan melalui penelusuran spesies rujukan di situs IUCN (International Union for the Conservation of Nature) Red list. Hasil identifikasi molekuler menunjukan bahwa sampel sirip hiu dari  kota Manado  memiliki kemiripan yang tinggi dengan spesies : Carcharhinus falciformis  (HM1 dan HM2) dan C. melanopterus (HM3).  Menurut data IUCN Red list, C. falciformis dan C. Melanopterus merupakan jenis hiu dalam status konservasi rentan punah (VU). Kata Kunci: Molekuler; COI; hiu; Manado dan IUCN Red list
Isolation and Antibacterial Activity assay of Endophytic Symbiont Bacteria on Seaweed Gracilaria verrucosa originated from Batu Meja Tongkaina Beach, North Sulawesi Sirri, Yolanda; Warouw, Veibe; Rumengan, Inneke Fenny; Paransa, Darus Sa'adah; Undap, Suzanne Lydia; Ginting, Elvy Like
Jurnal Ilmiah Platax Vol. 10 No. 2 (2022): ISSUE JULY-DECEMBER 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Seaweed can produce bioactive compounds such as antibacterial. Seaweed co-exists with bacteria endophytes. Entophytic bacteria are bacteria that live in host tissues and have the ability to protect the host itself by producing antibacterial compounds against pathogens. Therefore, the endophytic bacteria of seaweed symbionts can be utilized to produce antibacterial compounds. Bacteria can be mass-cultured because of their fast-growing characteristic. The aim of this study was to determine the antibacterial activity from endophytic symbiont bacteria on seaweed Gracilaria verrucosa originated from Batu Meja Tongkaina Beach, North Sulawesi. Isolation of bacteria was carried out by dilution steps from 101 to 10־3 times, then cultivation on Nutrient Agar media to obtain a single colony of the bacteria. Antibacterial activity was tested used paper disc diffusion method. The pathogens used were bacterial strains of Escherichia coli, Staphylococcus aureus, Streptococcus mutans, and Salmonella typhi. Moreover, antibiotics were used as positive controls. The antibacterial ability of the symbiotic bacteria was measured based on inhibition zones around the paper disc containing the isolates of endophytic bacteria. A total of 6 endophytic bacterial isolates were found, namely isolate B, C, D, E, F and G, characterized by different morphological features. The results showed that isolates B and C having inhibition zone of 0.5 – 1.0 mm against S. typhi, indicating that these two isolates produce antibacterial compounds with a weak ability  against S. typhi.
Antibacterial Activity of Endophytic Bacteria of Seagrass Symbiont Enhalus acoroides from Tiwoho Waters, North Minahasa Purniasih, Ni Komang Pitri; Ginting, Elvy Like; Wullur, Stenly; Mangindaan, Remy E. P.; Rumampuk, Natalie D. C.; Pratasik, Silvester Benny
Jurnal Ilmiah Platax Vol. 10 No. 2 (2022): ISSUE JULY-DECEMBER 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v10i2.42485

Abstract

Nutrient-poor marine bacteria are often found to form a living mechanism by associating with other marine organisms such as seagrass Enhalus acoroides. Seagrass serves as a habitat for marine biota and is known to produce bioactive compounds. Endophytic and epiphytic microbes that are symbiotic in seagrass have the ability to produce bioactive compounds similar to the bioactive compounds produced by their host. Therefore, this study aimed to obtain isolates of symbiont bacteria and to test their antibacterial activity.  The symbiotic bacteria isolates were tested for their antibacterial activity using gram-negative bacteria such as E. coli and S. typhi, and gram-positive bacteria such as S. aureus and S. mutans. Antibacterial activity test was carried out using the paper disc method. Observation of antibacterial activity was carried out for 3x24 hours by observing the growth of the resulting inhibition zone. The zone of inhibition was measured to determine the diameter and strength of the bioactive compounds produced by symbiotic bacteria.A total of six isolates of symbiotic bacteria were obtained from the seagrass Enhalus acoroides with varied morphological characteristics. The six isolates of symbiont bacteria showed antibacterial activity and could inhibit and kill pathogenic bacteria. Two of the six isolates of symbiotic bacteria that produced the largest diameter of the inhibition zone were isolates E (Ep1) 3,5 mm and F (En3) 4 mm in S. mutans test bacteria.Keywords: Antibacterial, Isolation, Enhalus acoroides, Symbionts, Tiwoho AbstrakBakteri laut yang miskin nutrisi banyak dijumpai membentuk mekanisme hidup dengan cara berasosiasi dengan organisme laut lainnya seperti lamun Enhalus acoroides. Lamun berfungsi sebagai habitat biota laut dan diketahui dapat menghasilkan senyawa bioaktif. Mikroba endofit dan epifit yang bersimbiosis pada lamun mempunyai kemampuan untuk memproduksi senyawa-senyawa bioaktif yang serupa dengan senyawa bioaktif yang diproduksi inangnya. Oleh karena itu, penelitian ini bertujuan untuk mendapatkan isolat bakteri simbion serta menguji aktivitas antibakterinya.  Isolat bakteri simbion diuji aktivitas antibakterinya menggunakan bakteri uji gram negatif seperti E. coli dan S. typhi, dan bakteri uji gram positif seperti S. aureus dan S. mutans. Uji aktivitas antibakteri dilakukan menggunakan metode kertas cakram. Pengamatan aktivitas antibakteri dilakukan selama 3x24 jam dengan mengamati pertumbuhan zona hambat yang dihasilkan. Zona hambat diukur untuk mengetahui diameter dan kekuatan senyawa bioaktif yang dihasilkan oleh bakteri simbion.  Sebanyak enam isolat bakteri simbion yang didapatkan dari lamun Enhalus acoroides dengan karakteristik morfologi yang bevariasi. Keenam isolat bakteri simbion menunjukkan adanya aktivitas antibakteri dan dapat menghambat serta membunuh bakteri pathogen. Dua dari enam isolat bakteri simbion menghasilkan diameter zona hambat terbesar adalah isolat E (Ep1) 3,5 mm dan F (En3) 4 mm pada pada bakteri uji S. mutans.Kata kunci: Antibakteri, Isolasi, Enhalus acoroides, Simbion, Tiwoho
Community structure and distribution of Gastropoda in the intertidal area of the Waleo districts coast north Minahasa Kahembau, Vinawati; Lalita, Jans Djoike; Rangan, Jety K.; Ginting, Elvy Like; Mantiri, Rose O.S.E.; Moningkey, Ruddy D.
Jurnal Ilmiah Platax Vol. 11 No. 2 (2023): ISSUE JULY-DECEMBER 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i2.45979

Abstract

This research is to know community structure and gastropod distribution include species density (individual/m2), relative density (%), diversity indices (H’), Evenness indices (E), Richness indices (R), Dominance indices (D) and Dispersion indices (Id).This inestigation was carried out February 2020 in intertidal area, Waleo beach in Minahasa Northern. Quadrat transect placed via area sampling based on substrate difference. Placing quadrat transect was centr on three points that different with distance with point 100 m and distance between quadran 10 m with size quadrat 50 cm2. Based on this research was find 22 species include Cypraea tigris, Terebra areolata, Cymbiola nivosa oblita, Euplica borealis, Turbo argyrostomus, Cypraea moneta, Conus cumingii, Cypraea errones, Tectus fenestrats, Nerita ascensionis, Nerita exuvi, Nerita albicilla, Nerita polita, Nerita Picea, Vasum turbinellus, Lunella cinerea, Nassarius globosus, Nassarius pullus, Vexillum acupictum, Clypeomorus pellucida, Vexillum ebenus, Terebralia sulcata. Five order include Neogastropoda, Littorinimorpha, Cycloneritida, Caenogastropoda, Trochida. Analysis result to species density is the highest 0,40 (individual/m2),relative density, Nerita polita 12,63 %, index of diversity,H’= 3,…, index of evenness, E = 0,97, index of richness, R = 0,22 and index of dominance, range D =0,13-0,18 concluded that no species dominantly. Gastropoda distribution pattern in intertidal area based 3 stations that showed all pattern of clumed distribution. Keywords: Gastropoda, Intertidal, density, spread pattern Abstrak Penelitian ini bertujuan untuk mengetahui struktur komunitas dan pola penyebaran gastropoda meliputi Kepadatan spesies (Ind/m2), Kepadatan Relatif (%),Indeks Keanekaragaman (H’), Indeks Keseragaman (E), Indeks Kekayaan spesies (R), Indeks Dominansi (D), dan indeks Sebaran. Penelitian ini di lakukan pada bulan februari 2020 yang berlokasi di daerah intertidal pantai Waleo Kabupaten Minahasa Utara. Penempatan transek kuadran di tentukan melalui teknik area sampling berdasarkan perbedaan substrat, penempatan transek dan kuadran di pusatkan di tiga titik yang berbeda-beda dengan jarak antara titik 100m dan jarak antara kuadran 10m dengan ukuran kuadran 50x50cm. Dari hasil penelitiaan di temukan 22 spesis meliputi Cypraea tigris, Terebra areolata, Cymbiola nivosa oblita, Euplica borealis, Turbo argyrostomus, Cypraea moneta, Conus cumingii, Cypraea errones, Tectus fenestrats, Nerita ascensionis, Nerita exuvi, Nerita albicilla, Nerita polita, Nerita Picea, Vasum turbinellus, Lunella cinerea, Nassarius globosus, Nassarius pullus, Vexillum acupictum, Clypeomorus pellucida, Vexillum ebenus, Terebralia sulcata. Dan 5 ordo Neogastropoda, Littorinimorpha, Cycloneritida, Caenogastropoda, Trochida. Hasil analisis terhadap kepadatan spesis nilai tertinggi terdapat pada spesis N.polita sebesar 0,40 Ind/m² dan kepadatan realtif tertinggi juga terdapat pada spesis N.polita sebesar 12,63 %, nilai indeka keanekaragaman H’= 9,49 yang merupakan kategori tinggi, nilai dari indeks keseragaman secara keseluruhan (E)=0,97 dalam kondisi , dan nilai indeks kekayaan spesis yaitu 0,22, indeks Dominansi (D) = 0,13 – 0,18 disimpulkan tidak ada yang mendominasi, Pola sebaran gastropoda di daerah intertidal dari ketiga stasiun menunjukan pola sebaran secara mengelompok. Wilayah pantai Waleo Kabupaten Minahasa Utara memiliki suhu 31,3ºC. Derajat keasaman pH yang di peroleh yaitu 7 Salinitas yang di peroleh sebesar 34‰ yang masih dalam kisaran baik untuk gastropoda. Kata kunci : Gastropoda, intertidal, kepadatan, pola sebaran
Strategy for Sustainable Use of Mangrove Ecosystem In Coastal Tatengesan, Southeast Minahasa Regency Pamungkas, Yogo; Paruntu, Carolus Paulus; Rumengan, Antonius Petrus; Manembu, Indri Shelovita; Sondak, Calvyn Fredrik Aldus; Ginting, Elvy Like
Jurnal Ilmiah Platax Vol. 12 No. 1 (2024): ISSUE JANUARY-JUNE 2024
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v12i1.51570

Abstract

The mangrove ecosystem area in the coastal area of Tatengesan Village, Pusomaen District, Southeast Minahasa Regency has been developed as a mangrove ecotourism area which has been managed by Village-Owned Enterprises (BUMD) since January 2019 with assistance from the Mandiri Rural National Community Empowerment Program (PNPM) team. The development of this mangrove ecotourism activity was stopped for a moment due to the impact of the COVID-19 pandemic, but currently, COVID-19 cases are considered endemic so this mangrove ecotourism activity will be developed again. This research aims to formulate some strategies for the sustainable use of the mangrove ecosystem on the Tatengesan coast, Southeast Minahasa Regency. This research lasted for 5 months, from September 2022 - February 2023. This research used descriptive qualitative methods with survey technique, i.e., in-depth direct interviews with resource persons (respondents). The formulation of priority strategies (key success factors) was analyzed using S.W.O.T and strategy choice analysis. This research produced 9 (nine) priority strategies as academic texts, which can be recommended to governments related to the marine and fisheries sector as elements of public policy-making officials. Keywords: Tatengesan village, mangrove ecotourism, sustainable use, priority strategies. Abstrak Kawasan ekosistem mangrove di wilayah pesisir Desa Tatengesan, Kecamatan Pusomaen, Kabupaten Minahasa Tenggara dikembangkan sebagai kawasan ekowisata mangrove yang dikelola oleh Badan Usaha Milik Desa (BUMD) sejak Januari 2019 dengan pendampingan oleh tim Program Nasional Pemberdayaan Masyarakat (PNPM) Mandiri Perdesaan.  Kegiatan ekowisata mangrove ini pengembangannya terhenti sejenak dikarenakan adanya dampak pandemi Covid-19, namun saat ini kasus Covid-19 sudah dianggap endemi sehingga kegiatan ekowisata mangrove ini akan dikembangkan kembali.  Tujuan penelitian ini yaitu merumuskan strategi pemanfaatan berkelanjutan ekosistem mangrove ke arah berkelanjutan di pesisir Tatengesan Kabupaten Minahasa Tenggara.  Riset ini berlangsung selama 5 bulan, dari September 2022 - Februari 2023.  Riset ini menggunakan metode kualitatif deskriptif dengan teknik survei, yaitu wawancara langsung secara mendalam (in-depth interview) kepada narasumber (responden).  Perumusan strategi prioritas (faktor kunci keberhasilan) di analisis dengan menggunakan S.W.O.T dan analisis pilihan strategi.  Penelitian ini menghasilkan 9 (sembilan) strategi prioritas sebagai naskah akademik, yang dapat direkomendasikan kepada pemerintah terkait pada sektor kelautan dan perikanan sebagai unsur pejabat pembuat kebijakan publik. Kata kunci: Desa Tatengesan, ekowisata mangrove, pemanfaatan berkelanjutan, strategi prioritas.
Reviewing coastal culture and traditions for English teaching materials Warouw, Maya Pinkan; Ginting, Elvy Like; Tondo, Henry Fanny
International Journal of Evaluation and Research in Education (IJERE) Vol 13, No 6: December 2024
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijere.v13i6.28597

Abstract

Women’s empowerment can be encouraged via English teaching and teaching materials can be utilized to strengthen gender equality and women’s empowerment, particularly for students from coastal areas pursuing higher education at universities. This research aims to determine how English language teaching (ELT) materials in universities can be adapted and incorporated into English instruction from the perspective of female students from coast. The data for this study were acquired using a qualitative technique, which included the distribution of questionnaires and interviews with female students from coastal locations. The information gathered from the questionnaire was mostly demographic. In the meanwhile, interviews were held to collect thoughts from participants on coastal traditions and cultures that might be included into teaching materials. The findings reveal a clear correlation between individuals’ perspectives and their experiences shaped by traditions and cultural heritage of coastal regions. Female participants highlight the depth of coastal culture, it is interaction with the environment, the crucial role of women in preserving traditions, and the desire for representation in university-level English teaching materials. These insights, derived from the experiences and viewpoints of female students in a coastal setting, extend beyond their locale, offering valuable lessons for English learning approaches in diverse coastal communities
Isolation and Potential of Plastic-Degrading Bacteria from Plastic Waste Fatti, Clara; Rumampuk, Natalie Detty C.; Gerung, Grevo Soleman; Wullur, Stenly; Mamuaja, Jane Marianne; Ginting, Elvy Like
Jurnal Ilmiah Platax Vol. 13 No. 1 (2025): ISSUE JANUARY-JUNE 2025
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v13i1.58358

Abstract

Plastic waste is an environmental issue, including in marine environments. One method of managing plastic waste is through biodegradation using bacteria. This study aims to isolate bacteria from plastic waste in Malalayang, and North Sulawesi waters, and test their potential to degrade plastic using laboratory experimental methods. Bacteria were isolated using a Nutrient Agar (NA) medium and then tested for their ability to degrade plastic using a Nutrient Broth (NB) medium containing plastic fragments. The NB medium with plastic fragments was incubated using an orbital shaker at room temperature with agitation at 130 rpm for 30 days. Five bacterial isolates were successfully obtained from plastic waste: isolates C1, C2, C3, C4, and C5. Four of the bacterial isolates were found to be capable of degrading plastic, as indicated by the reduction in the dry weight of the plastic after 30 days of incubation. The highest plastic weight reduction was shown in isolate C2, with a decrease of 2.95%, while the lowest reduction was observed in isolate C4, with a decrease of 2%. Keywords: Bacteria, Isolation, Biodegradation, Plastic. Abstrak Sampah plastik merupakan masalah lingkungan termasuk lingkungan laut. Salah satu pengelolaan sampah plastik adalah melalui biodegradasi menggunakan bakteri. Penelitian ini bertujuan mengisolasi bakteri dari sampah plastik Perairan Malalayang, Sulawesi Utara dan melakukan uji potensi bakteri tersebut dalam mendegradasi plastik menggunakan metode eksperimen laboratorium. Bakteri diisolasi menggunakan medium Nutrient Agar (NA), kemudian diuji kemampuan mendegradasi plastik dengan menggunakan medium Nutrient Broth (NB) yang berisi potongan plastik. Medium NB berisi potongan plastik diinkubasi menggunakan orbital shaker pada suhu ruang dengan agitasi 130 rpm selama 30 hari. Lima isolat bakteri berhasil diisolasi dari sampah plastik, yaitu isolat C1, C2, C3, C4 dan C5. Didapatkan empat isolat bakteri yang mampu mendegradasi plastik, yang ditunjukkan dengan pengurangan berat kering plastik setelah diinkubasi selama 30 hari. Pengurangan berat plastik tertinggi ditunjukkan oleh isolat C2 sebesar 2,95 %, sedangkan pengurangan berat terendah oleh isolat C4 sebesar 2 %. Kata kunci: Bakteri, Isolasi, Biodegradasi, Plastik.
Blister Pearl Formation in Abalone, Haliotis varia Using Anestesia Gulo, Puji Eli Arnita; Mamangkey, Noldy Gustaf Frans; Ompi, Medy; Manembu, Indri Shelovita Manembu; Kawung, Nickson J.; Ginting, Elvy Like; Angkouw, Esther D.
Jurnal Ilmiah Platax Vol. 13 No. 1 (2025): ISSUE JANUARY-JUNE 2025
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v13i1.60568

Abstract

Abalone pearl farming has significant potential to improve the livelihoods of coastal communities. This study aimed to measure early pearl blister layer thickness and analyze mortality in Haliotis varia following nucleus insertion. A four-month experimental study was conducted in Pulisan, North Sulawesi, Indonesia. Forty abalones underwent nucleus insertion using quick-drying adhesive after being anesthetized with benzocaine (1200 mg/L). Samples were collected monthly for two months to measure pearl layer thickness. Results showed that the average pearl layer thickness increased from 0.093 mm in the first month to 0.145 mm in the second month. However, mortality was highest in the treatment group (30%), followed by the control group with anesthesia (20%), and the control group without treatment (5%). This study concludes that pearl formation in H. varia is continuous but that insertion and anesthesia procedures stress individuals. These findings provide a foundation for developing more efficient and sustainable abalone pearl farming techniques. Keywords: Pearl, Pearl Production, Abalone, Haliotis varia, Anestesia. Abstrak Produksi mutiara abalone memiliki potensi besar untuk meningkatkan kesejahteraan masyarakat pesisir. Penelitian ini bertujuan untuk mengukur pertumbuhan awal lapisan mutiara blister dan menganalisis mortalitas pada abalone Haliotis varia setelah proses insersi inti mutiara. Penelitian eksperimental ini dilakukan selama 4 bulan di perairan Pulisan, Sulawesi Utara. Sebanyak 40 individu abalone diinsersi inti mutiara menggunakan lem cepat kering setelah dibius dengan benzocaine (1200 mg/L). Sampel diambil setiap bulan selama dua bulan untuk mengukur ketebalan lapisan mutiara yang terbentuk. Hasil penelitian menunjukkan bahwa rata-rata ketebalan lapisan mutiara meningkat dari 0,093 mm pada bulan pertama menjadi 0,145 mm pada bulan kedua. Namun, mortalitas tertinggi (30%) terjadi pada kelompok perlakuan insersi, diikuti oleh kelompok kontrol dengan perlakuan anestesi (20%) dan kelompok kontrol tanpa perlakuan (5%). Studi ini menyimpulkan bahwa proses pembentukan mutiara pada H. varia berlangsung terus-menerus, namun prosedur insersi dan anestesi memberikan tekanan pada individu abalone. Penelitian ini memberikan dasar penting untuk pengembangan teknik produksi mutiara abalone yang lebih efisien dan berkelanjutan. Kata kunci: Mutiara, Produksi Mutiara, Abalone, Haliotis varia, Anestesi.
Co-Authors Angkouw, Esther D. Calvyn F. A. Sondak, Calvyn F. A. Carolus Paulus Paruntu Deiske Adeliene Sumilat, Deiske Adeliene Fatti, Clara Grevo S Gerung Gulo, Puji Eli Arnita Henky Manoppo Hens Onibala Indri Manembu Inneke F. M Rumengan Kahembau, Vinawati Lalita, Jans Djoike Maarisit, Ismariani Mamangkey, Noldy Gustaf Frans Mamuaja, Jane Marianne Manembu, Indri Shelovita Manembu Mantiri, Rose O.S.E. Medy Ompi Moningkey, Ruddy D. Mopay, Maratade Natalie D Rumampuk Nickson J. Kawung, Nickson J. Paransa, Darus Sa'adah Pratasik, Silvester Benny Purniasih, Ni Komang Pitri Rangan, Jety K. Remy E. P Mangindaan Robert A. Bara Rumampuk, Natalie Detty C. Rumengan, Antonius Petrus Rumengan, Inneke Fenny Salaki, Meiske S. Sembiring, Sindiy Cloudya Sirri, Yolanda Stenly Wullur Tondo, Henry Fanny Undap, Suzanne Lydia Veibe Warouw WAROUW, MAYA PINKAN Yogo Pamungkas, Yogo