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Umboh, Stella D.
Universitas Sam Ratulangi
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Journal : Jurnal Bios Logos

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Tingkat Populasi Jamur Tanah akibat Perlakuan Fungisida Mankozeb di Pertanaman Sayur Kubis (Brassica oleracea var.capitata) Kecamatan Modoinding, Kabupaten Minahasa Selatan, Sulawesi Utara (The Population Level of Soil Fungi under Mankozeb Fungicides Application in the Cabbage (Brassica oleracea var.capitata) Plantation of Modoinding Subdistrict, South Minahasa District, North Sulawesi) Lestari, Isna; Umboh, Stella D.; Pelealu, Johanis J.
JURNAL BIOS LOGOS Vol 8, No 1 (2018): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.8.1.2018.20594

Abstract

Abstrak Dalam pengembangan sayuran kubis (Brassica oleracea var.capitata) petani sering menggunakan pestisida secara berlebihan. Penggunaan pestisida secara berlebihan tersebut berdampak pada sterilisasi ekosistem tanah, yang mengakibatkan penurunan tingkat populasi jamur tanah. Tujuan penelitian ini yaitu untuk menganalisis tingkat populasi jamur tanah akibat paparan fungisida Mankozeb di pertanaman sayuran kubis. Penelitian ini dilakukan dengan mengisolasi jamur pada tanah di sayur kubis Kecamatan Modoinding, Kabupaten MInahasa Selatan menggunakan pengenceran bertingkat, kemudian dilanjutkan dengan metode Total Plate Count (TPC) untuk menghitung jumlah koloni. Hasil penelitian menunjukkan bahwa fungisida Mankozeb mempengaruhi jumlah populasi jamur tanah, dilihat dari sedikitnya jumlah spesies yang ditemukan. Dari 6 famili dengan 11 jenis yang ditemukan, jamur tanah yang memiliki koloni terbesar adalah isolat KJ5 (Penicillium sp.) sebesar 270 koloni dengan  jumlah spora 5.9x10-6 CFU/mL  dan terendah pada isolat KJ3 (Trichoderma harzianum) sebesar 2 koloni dengan jumlah spora 10-7 CFU/mL.Kata kunci : Kubis, jamur tanah, Mankozeb, koloni, TPC, CFU/mL Abstract              Farmers often use excessive pesticides in the plantation of cabbage (Brassica oleracea var. capitata). The excessive use of pesticides resulted in sterilization of soil ecosystems and followed by the decrease the level of soil fungal population. The purpose of this study was to analyze the population level of soil fungi after exposure the cabbage plantation to Mankozeb fungicide. This research was conducted by isolating the fungi from the soil in cabbage plantation in Modoinding subdistrict, South Minahasa District using a multilevel dilution and followed by  Total Plate Count (TPC) method to calculate the fungi colony number. The results showed that Mankozeb fungicide affected the population of soil fungi, based on the limited number of identified fungi species. There were 6 families and 11 species of soil fungi. The largest number was in KJ5 (Penicillium sp.), i.e. 270 colonies and the spore number was 5.9x10-6 CFU/mL. The lowest number was KJ3 (Trichoderma harzianum), i.e. 2 colonies and the number of spores was 10-7 CFU / mL.Keywords : Cabbage, soil fungi, Mankozeb, colony, TPC, CFU/mL
Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro) Dotulong, Ghea; Umboh, Stella; Pelealu, Johanis
JURNAL BIOS LOGOS Vol 9, No 2 (2019): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.9.2.2019.24746

Abstract

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro) Ghea Dotulong1*), Stella Umboh1), Johanis Pelealu1), 1) Program Studi Biologi, FMIPA Universitas Sam Ratulangi, Manado 95115*Email korespondensi: dotulong.ghea@gmail.com Diterima 9 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 Abstrak Tanaman kentang (Solanum tuberosum L.) adalah salah satu tanaman hortikultura yang sering mengalami penurunan dari segi produksi dan produktivitasnya, akibat adanya serangan penyakit layu yang salah satunya disebabkan oleh Fusarium oxysporum. Tujuan penelitian ini adalah mengidentifikasi toksisitas beberapa fungisida nabati dalam mengendalikan penyakit Layu Fusarium (F. oxysporum) pada tanaman kentang (Solanum tuberosum L.) secara In Vitro. Metode Penelitian yang digunakan yaitu metode umpan beracun. Data dianalisis dengan Rancangan Acak Lengkap (RAL) dengan Analisis Varian (ANAVA) yang dilanjutkan dengan menggunakan metode BNT (Beda Nyata Terkecil). Hasil Penelitian, diperoleh nilai toksisitas fungisida nabati tertinggi yaitu pada ekstrak daun sirsak dengan nilai HR (69,44%), kategori berpengaruh, ditandai dengan diameter koloni 2,75 cm (100ppm) dan yang terendah toksisitasnya yaitu pada ekstrak daun jeruk purut dengan nilai HR (49,81%), kategori cukup berpengaruh ditandai dengan diameter koloni 3,75 cm (25ppm). Semakin tinggi konsentrasi yang diujikan maka semakin tinggi toksisitas dari fungisida nabati yang diberikan.Kata Kunci: fungisida nabati, Fusarium oxysporum, tanaman kentang, In Vitro Abstract Potato plants (Solanum tuberosum L.) is one of the horticulture plants which often decreases in terms of production and productivity, due to the attack of wilt, one of which is caused by Fusarium oxysporum. The purpose of this study was to determine the toxicity of several biofungicides in controlling Fusarium wilt (F. oxysporum) in potato plants (Solanum tuberosum L.) in Vitro. The research method used was the In Vitro method with the poison bait method. Data were analyzed by Completely Randomized Design with Variant Analysis (ANAVA), followed by the BNT method. The results showed that the highest biofungicide toxicity value was soursop leaf extract with HR values (69.44%), influential categories, characterized by colony diameter 2.75 cm (100ppm) and the lowest toxicity, namely in kaffir lime leaf extract with a value of HR (49.81%), quite influential category was characterized by colony diameter of 3.75 cm (25ppm). The higher the concentration tested, the higher the toxicity of the biofungicide given.Keywords: biofungicides, Fusarium oxysporum, Potato Plants, In Vitro. 
In Vitro Evaluation of the Antagonism of Saprophyte and Endophytic Fungi Isolated from Groundnut (Arachis hypogaea) Against Soil-Transmitted Diseases Sclerotium rolfsii Johanis Pelealu; Edwin de Queljoe; Lalu Wahyudi; Stella Deiby Umboh; Trina Tallei
JURNAL BIOS LOGOS Vol. 13 No. 1 (2023): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v13i1.46484

Abstract

Certain soil-borne diseases can have a detrimental effect on the quality and quantity of peanut production. One of the soil-borne diseases is Sclerotium rolfsii, a fungus that is the primary limiting factor in peanut plants. An alternative to controlling this fungus is to use saprophytic and endophytic fungi. The purpose of this study was to evaluate several saprophytic and endophytic fungi isolated from peanut plants in vitro as antagonistic agents against S. rolfsii. Isolation of saprophytic and endophytic fungi was carried out using the stratified dilution method. The fungal antagonism test was carried out using a non-factorial completely randomized design with 3 replications. The parameters observed in this study included the inhibiting zone, the diameter of the isolate colonies, the growth area, and the interaction of saprophytic and endophytic fungi with S. rolfsii. Five families and eight species of soil fungi were identified using macroscopic and microscopic identification techniques. The type of soil fungus has a large impact on the growth rate and inhibition area. Mucor hiemalis grew at the fastest rate, while Rhizopus oryzae had the largest inhibition zone.
Co-Authors Arantika, Windha Bawole, Kevin V. Deidy Y. Katili Deidy Yulius Katili Dotulong, Ghea Edwin de Queljoe Hanny Pontororing Johanis J. Pelealu Johanis Pelealu Johanis Pelealu Lalu Wahyudi Lalu Wahyudi Lalu Wahyudi Lestari, Isna Marhaenus Rumondor Marnix L. D Langoy Marnix L.D Langoy Marnix Langoy Meytij Jeanne Rampe Patrycia Saskia Laurita Supit Pelealu, Johanis J. Rampe, Henny Rampe, Henny Lieke Rampe, Henny Like Rumondor, Marhaenus Johanis Song Ai Nio Sumeisey, Gabriella Sumeisey, Gabriella Nathalie Trina E. Tallei, Trina E. Trina Tallei Windha Arantika