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All Journal Jurnal Mandala Pharmacon Indonesia ad-Dawaa : Journal of Pharmaceutical Sciences Jurnal farmasi UIN Alauddin Makassar Molekul: Jurnal Ilmiah Kimia
Doloking, Haeria
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Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Development and Validation of Zerumbone Analysis Method Using Ultraviolet-Visible Spectrophotometry Doloking, Haeria; Muhlisuddin, Muhlisuddin; Maddeppungeng, Nurul Muhlisah
Molekul Vol 20 No 3 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.3.15690

Abstract

ABSTRACT. Analytical method development ensures that an analytical procedure is suitable for the identification and quantification of compounds of interest. This study aimed to develop and validate a simple, cost-effective analytical method for the determination of zerumbone using UV-Vis spectrophotometry. Method validation was conducted according to ICH Q2(R2) guidelines, including tests for specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy, and precision. Zerumbone exhibited a maximum absorption wavelength (λmax) of 212 nm when dissolved in dimethyl sulfoxide (DMSO). The method demonstrated excellent linearity with a determination coefficient (R2) of 0.9991 across a concentration range of 10-50 μg/mL. Accuracy, evaluated via recovery studies, ranged from 91,00% to 110,45%. Precision testing results in a % RSD of 0.58%. The calculated LOD and LOQ were 1.55 μg/mL and 4.70 μg/mL, respectively. This validated method is simple, rapid, and economical, making it a promising alternative for zerumbone analysis in quality control laboratories. Keywords: Zerumbone, Method Development, UV-Vis Spectrophotometry, Validation, ICH Guidelines.
Co-Authors Arif Setiawansyah Dwi Wahyuni Leboe Gita Susanti, Gita Hariratul Jannah Kairani, Utari Estina Lallo, Subehan Lalo, Ahmad Ludgensia Indradewi Kurniati Luthfiana, Dewi Maddeppungeng, Nurul Muhlisah Maharani, Andi Rani Gustia Mantur Yudokus Marianti A Manggau Muhlisuddin, Muhlisuddin Mukhriani Nasrawati Basir Ningsi, Surya Nur Aeti Nurwana Nweze, Leonard Chinecherem Putri, Stella Anatasya Putri Suprapto Prayitno Surya Ningsi Tahar, Nurshalati Tahir, Karlina Amir Wahyuddin, Munifah Yusnita Rifai, Yusnita