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Erpi Nurdin
Jurusan Analis Kesehatan, Politeknik Kesehatan Ternate, Jln. Cempaka, Ternate, Maluku Utara
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences

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Comparison of Tuberculosis Examination Using Ziehl-Neelsen Method and Molecular Rapid Test Anwar, Aan Yulianingsih; Handayani, Marini Tri Putri; Nurdin, Erpi; Lewa, Irma Berliana,HI; Artati, Artati
Jurnal Kesehatan Manarang Vol 9 No 3 (2023): December 2023
Publisher : Politeknik Kesehatan Kemenkes Mamuju

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33490/jkm.v9i3.848

Abstract

Tuberculosis is an infectious disease of the respiratory tract caused by infection with the bacteria Mycobacterium tuberculosis. Fast and accurate diagnostic methods are very important for diagnosing and controlling pulmonary TB disease in Indonesia. TB examination using the Zn method is very commonly used even though it has lower sensitivity than TCM. The aim of this study was to compare the results of TB examination using BTA staining with the Molecular Rapid Test. This type of research uses analytical observational methods with a cross-sectional approach. The sample was 40 respondents taken using purposive sampling technique. The results of tuberculosis examination using the Ziehl-Neelsen (ZN) BTA staining method and the Moluker rapid test tool (TCM) method showed that the microscopic results of the Ziehl-Neelsen method of BTA smear staining showed negative results in 31 people (75%), 1 respondent (2 .5%) with scanty results and 9 respondents (22.5%) with 1+ results. The results of the TB BTA examination using the TCM method resulted in MTB Not Detected with a total of 30 people (75%), MTB Detected Medium obtained a result of 6 people (15%), MTB Detected Low obtained a result with a total of 3 people (7.5%), MTB Detected Very Low results were obtained with the number of 1 person (2.5%) and in the SPSS results using the t-test the results obtained were p value <0.05 (0.012 <0.05) which means there is a comparison of the BTA staining tuberculosis examination using the Ziehl-Ziehl method. neelsen and Molecular Rapid Tests. Based on the research results, it was found that there was a significant difference between the Ziehl-Neelsen BTA staining tuberculosis examination method and the Molecular Rapid Test Tool with a p value <0.05 (0.012 <0.05)
Deteksi Gen Resistensi Karbapenemase Dan Metallo-Β-Lactamase Pada Acinetobacter Baumannii Zuchrullah, Mukhtasyam; Nurdin, Erpi
Jurnal Media Analis Kesehatan Vol 15 No 1 (2024): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v15i1.631

Abstract

Peningkatan resistensi terhadap antibiotik golongan karbapenem merupakan salah satu fenomena yang harus diwaspadai. Acinetobacter baumannii merupakan salah satu bakteri penyebab infeksi berat yang didapatkan di rumah sakit. Penelitian ini bertujuan untuk mendeteksi gen intrinsik dan gen yang membawa resistensi obat golongan karbapenem terhadap A. baumannii. Penelitian ini dilaksanakan di Laboratorium HUM-RC RSPTN Universitas Hasanuddin Makassar. Desain penelitian ini adalah deskriptif eksperimental laboratorium dengan jumlah sampel 50 isolat yang didapatkan dengan cara total sampling yang dikumpulkan dari RSUP Wahidin Sudirohusodo Makassar. Data yang dianalisis adalah hasil karakteristik isolat, uji sensitivitas dengan menggunakan Vitek-2, uji genotipe gen intrinsik OXA-51 dan blaIMP-1 dengan menggunakan Polymerase Chain Reaction. Hasil penelitian menunjukkan bahwa dari total 50 isolat A. baumannii, ditemukan paling banyak pada sampel sputum sejumlah 30 isolat (60%). Dari total 50 sampel uji sensitivitas antibiotik, isolat A. baumanni paling banyak resisten terhadap meropenem 14 isolat (28%) kemudian imipenem 9 isolat (18%), dan Doripenem 9 isolat (18%). Gen intrinsik OXA-51 ditemukan pada semua isolat sampel sedangkan hanya satu isolat dari A. baumannii yang mampu memproduksi enzim metallo-β-Lactamase (MBL) dan membawa gen blaIMP-1 yaitu pada sampel urine yang seluruhnya resisten terhadap ketiga golongan obat karbapenem. Kata Kunci : Acinetobacter baumannii, Karbapenem, MBL, Polymerase Chain Reaction
PEMANFAATAN MEDIA ALTERNATIF DALAM ISOLASI BAKTERI PADA URINE PENDERITA INFEKSI SALURAN KEMIH DI WILAYAH DAERAH BINAAN KELURAHAN DUFA-DUFA KOTA TERNATE Erpi Nurdin; Jakaria, Febrianti; Hasanuddin, Riskawati
Jurnal Abdi Insani Vol 11 No 3 (2024): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v11i3.1775

Abstract

Bacteriological examination, especially bacterial culture, is the gold standard for infectious diseases. The very limited availability of culture media encourages the use of alternative media, especially to see the presence of bacteria in the urine of patients with Urinary Tract Infections (UTIs). The price of semi-synthetic culture media is quite expensive and it is difficult for producers to obtain it in the North Maluku region, especially in Ternate City. The use of this alternative media is expected to provide direct benefits to detect the presence of bacteria through the urine of patients with UTIs and improve the knowledge and skills of ATLM cadres regarding the procedure for using alternative media. The use of tuna as one of the alternative media ingredients has not been optimal for the purposes of microbiology laboratory diagnostics. Carried out in the form of socialization which is implemented in the form of lectures, presentations of materials by the community service team with material on the use of alternative media for examining bacteria that cause UTIs. This activity also involves the activeness of participants by working on assessment worksheets and ending with a post-test activity. In the form of knowledge and application of the Use of alternative culture media based on tuna in examining urinary tract infections in the Dufa-Dufa Village community. The test results showed an increase in the percentage of understanding of community service participants, where at the beginning of the activity there were 54% who did not understand UTI and microbiology laboratory examinations, after receiving education there were no more participants who did not understand. In the choice of understanding enough at the beginning of the test 42% and post test 0%. From the criteria understood at the beginning with only 4%, increased to 48%. In the criteria very understood from 0% increased to 52%. The results of the UTI examination through urine culture with alternative media were that there was UTI in 32 people (64%) and the possibility of UTI in 18 people (36%). Increased understanding of the importance of conducting urine culture examinations related to UTI up to 100%, where 48% understood and 52% understood very much. There were 64% of participants with UTI and the possibility of UTI 36%.
BACTERIAL CULTURE PROFILE ON THROAT SWAP OF PATIENTS WITH ACUTE RESPIRATORY TRACT INFECTION AT KALUMPANG HEALTH CENTER TERNATE CITY Erpi Nurdin; Mukhtasyam Zuchrullah; Fifi B Umagapi
Jurnal Inovasi Pendidikan dan Sains Vol 4 No 3 (2023): December
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Nahdlatul Wathan Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51673/jips.v4i3.1899

Abstract

Abstract: Respiratory tract infection is a common disease in society, and is one of the highest causes of death in children under 5 years of age (22.30%). Acute respiratory tract infections are divided into lower respiratory tract infections and upper respiratory tract infections. This infectious disease can be carried out by bacteriological examination using selective media to see the bacterial species. The aim of the research was to determine the bacterial culture profile in throat swabs of patients with acute respiratory infections at the Kalumpang Community Health Center, Ternate City using selective media, biochemical tests and gram staining. Research conducted using descriptive methods. Based on data on the characteristics of the Acute Respiratory Infection (ARI) patients studied, there were 17 men (57%) and 13 women (43%), while the age group with the most sufferers was in the 1-10 year age range, namely 9 people (30%) and then in the age range >50 years, namely 8 people (27%). Meanwhile, the percentage of culture profile findings from the bacterial species Stapylococcus aureus was 50%, Streptococcus pygones 33%, Pseudomonas aeruginosa 7%, Corynebacterium diphtheriae 10%. Found 98% gram positive bacteria and 2% gram negative bacteria. From the research results, it can be concluded that the most common bacterial culture profile in the throat swabs of upper respiratory tract infection patients is gram-positive bacteria consisting of the species Stapylococcus aureus, Streptococcus pygones, and Corynebacterium diphtheriae. The gram negative bacterial profile obtained was Pseudomonas aeruginosa. Keywords : Acute Respiratory Infections, throat swab, culture, bacteria
Co-Authors Aan Yulianingsih Anwar Artati, Artati Fifi B Umagapi Halik, Sofyanti Abdul Handayani, Marini Tri Putri Hasanuddin, Riskawati Hi Lewa, Irma B Irma B. Hi. Lewa Jakaria, Febrianti Lewa, Irma B. Hi Lewa, Irma Berliana,HI Mukhtasyam Zuchrullah Nasarudin, Sandi Nofita Sari Djurumudi Puasa, Rony Rafika Rafika Suanbani, Dawika Yulianingsih, Aan