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Journal : Andalasian Livestock

Effect of Inoculum Dose and Fermentation Time on Enzyme Activity and Nutrients of Palm Kernel Cake Fermented with Sclerotium rolfsii Powder Inoculum Fajrona, Kadran; Rizal, Yose; Mirnawati
Andalasian Livestock Vol. 1 No. 2 (2024): ALive
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/alive.v1.n2.p139-143.2024

Abstract

The study aimed to determine the inoculum dose and fermentation time of palm kernel cake with Sclerotium rolfsii powder inoculum on the enzyme activity of cellulase, mannanase, crude protein, and crude fiber. This study used an experimental method with a completely randomized design of a factorial 3x3 with two replications. Factor A is the inoculum dose consisting of A1: 6%, A2: 8%, and A3: 10%. Factor B is the fermentation time, which consists of B1: 5 days, B2: 7 days, and B3: 9 days. The measured parameters were cellulase and mannanase enzyme activity, crude protein, and fiber content. The data obtained were analyzed using ANOVA and continued with Duncan's multiple range test. The results show that the inoculum dose and fermentation time significantly affect (P<0.01) cellulase and mannanase enzyme activity, crude protein, and crude fiber content. The use of an inoculum dose of 8% and a fermentation period of 7 days gave the best results with an average cellulase enzyme activity of 26.77 U/ml, mannanase enzyme activity of 27.35 U/ml, crude protein of 24.07 U/ml and crude fiber of 19.26%.
Optimizing The Use of Fermented Palm Kernel Cake in Feed by Giving Humic Acid in Drinking Water on Broiler Performance Fajrona, Kadran; Ciptaan, Gita; Mirnawati
Andalasian Livestock Vol. 2 No. 2 (2025): ALive
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/alive.v2.n2.p107-112.2025

Abstract

This study aims to determine the dose of humic acid in drinking water to optimize the use of fermented palm kernel cake (FPKC) in broiler feed. The experimental design used was a completely randomized design (CRD) with 5 treatments (T1: 0% FPKC and 0% humic acid, T2: 25% FPKC + 100 ppm humic acid, T3: 25% FPKC + 200 ppm humic acid, T4: 30% FPKC + 100 ppm humic acid and T5: 30% FPKC + 200 ppm humic acid) and 4 replications. The variables observed were feed consumption, body weight gain, feed conversion, live weight, carcass percentage and abdominal fat percentage. The data obtained were analyzed by analysis of variance (ANOVA), if there was an effect between treatments it was continued with Duncan's multiple range test (DMRT). The results showed that the effect of humic acid in drinking water had no significant effect (P>0.05) on the T1, T2 and T3 treatments, but had a highly significant effect (P<0.01) on the T4 and T5 treatments. Giving humic acid in drinking water has not been able to optimize the use of FPKC up to 30%, but giving 100 ppm humic acid can optimize the use of FPKC as much as 25% in feed.
Effect of Inoculum Dose and Fermentation Time on Enzyme Activity and Nutrients of Palm Kernel Cake Fermented with Sclerotium rolfsii Powder Inoculum Fajrona, Kadran; Rizal, Yose; Mirnawati
Andalasian Livestock Vol. 1 No. 2 (2024): ALive
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/alive.v1.n2.p139-143.2024

Abstract

The study aimed to determine the inoculum dose and fermentation time of palm kernel cake with Sclerotium rolfsii powder inoculum on the enzyme activity of cellulase, mannanase, crude protein, and crude fiber. This study used an experimental method with a completely randomized design of a factorial 3x3 with two replications. Factor A is the inoculum dose consisting of A1: 6%, A2: 8%, and A3: 10%. Factor B is the fermentation time, which consists of B1: 5 days, B2: 7 days, and B3: 9 days. The measured parameters were cellulase and mannanase enzyme activity, crude protein, and fiber content. The data obtained were analyzed using ANOVA and continued with Duncan's multiple range test. The results show that the inoculum dose and fermentation time significantly affect (P<0.01) cellulase and mannanase enzyme activity, crude protein, and crude fiber content. The use of an inoculum dose of 8% and a fermentation period of 7 days gave the best results with an average cellulase enzyme activity of 26.77 U/ml, mannanase enzyme activity of 27.35 U/ml, crude protein of 24.07 U/ml and crude fiber of 19.26%.
Effect of Adding Palm Kernel Cake as a Substrate in Sclerotium rolfsii Inoculum Fajrona, Kadran; Rizal, Yose; Mirnawati
Andalasian Livestock Vol. 1 No. 1 (2024): ALive
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/alive.v1.n1.p78-83.2024

Abstract

This research aims to determine the added palm kernel cake (PKC) level in the Sclerotium rolfsii inoculum. This research used an experimental method with a completely randomized design, four treatments, and five replications. The treatments were different substrate compositions, consisting of 100% bran (control), 90% bran + 10% PKC (P1), 85% bran + 15% PKC (P2), and 80% bran + 20% PKC (P3). The variables observed were cellulase and mannanase enzyme activities. The data obtained were analyzed statistically using a completely random design analysis of variance and continued with Duncan's multiple range tests if the treatment influenced the observed variables. The results showed that adding PKC as a substrate in making Sclerotium rolfsii inoculum had a significant effect (P<0.01) on the activity of cellulase and mannanase enzymes. The best cellulase and mannanase enzyme activities were found in the 80% fine bran substrate composition + 20% PKC (P3), namely 16.02 U/mL and 7.81 U/mL.