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Setyo Widodo
PPPTMGB LEMIGAS, Balitbang KESDM, Jl. Ciledug Raya, Kav. 109, Cipulir, Kebayoran Lama, Jakarta, 12230, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Energy Engineering Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : Jurnal Veteriner

 1 
Profil Imunoglobulin-G Serum Kambing Peranakan Etawah Bunting yang Diberi Imbuhan Pakan Mineral Seng (SERUM IMUNOGLOBULIN-G LEVEL ON PREGNANT ETTAWAH CROSSBRED WERE GIVEN ZINC MINERAL) Sus Derthi Widhyari; Anita Esfandiari; I Ketut Sutama; Setyo Widodo; I Wayan Teguh Wibawan; Rizal Rahadian Ramdhany
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.664 KB) | DOI: 10.19087/jveteriner.2017.18.1.24

Abstract

The objective of this study was to find out the effect of zinc supplementation on the profile of immunoglobulin-G (IgG) in the pregnant etawah-cross ewes. Fifteen etawah-cross ewes (3-6 years old and 30-50 kg body weight) were used in this experiment and they were divided into three groups each of which consisted of 5 ewes. The first group (Zn40) received 40 ppm Zn as control, the second group (Zn60) received 60 ppm Zn, and the third group (Zn80) received 80 ppm Zn. Blood samples were collected from jugular vein every two weeks, started at twelve weeks of pregnancy up to eight weeks post partum for immunoglobulin-G analysis. IgG level were analyzed by competitive Enzyme Linked Immunosorbent Assay (ELISA) technique. Results showed that there were no significant difference of immunoglobulin-G level (P>0,05) among the three treatment groups ( Zn40, Zn60, and Zn80). However, IgG level of Zn40 and Zn80 groups tended to decrease during the last staget of pregnancy until two weeks post partum. In conclusion, supplementation of 60 ppm Zn showed higher IgG level than Zn 80 ppm. ABSTRAK Penelitian ini bertujuan untuk mengetahui efek suplementasi seng (Zn) terhadap imunoglobulin-G (IgG) pada kambing peranakan etawah (PE) bunting. Penelitian ini menggunakan 15 ekor kambing PE umur sekitar tiga sampai enam tahun, bobot badan sekitar 30-50 kg dibagi ke dalam tiga kelompok, masing-masing terdiri dari lima ekor. Kelompok Zn40 (kontrol) diberi pakan mengandung mineral Zn 40 ppm, kelompok Zn60 mengandung mineral Zn 60 ppm, dan kelompok Zn80 mengandung mineral Zn 80 ppm. Sampel darah diambil melalui vena jugularis untuk menganalisis konsentrasi IgG. Pengambilan sampel darah dilakukan setiap dua minggu mulai umur kebuntingan 12 minggu sampai delapan minggu setelah melahirkan. Hasil penelitian menunjukkan bahwa konsentrasi IgG serum tidak menunjukkan perbedaan secara nyata antar kelompok (P>0,05) antara kelompok Zn 0, Zn 40 dan Zn 80 ppm. Kelompok Zn 40 ppm dan 80 ppm cendrung memperlihatkan konsentrasi IgG mengalami penurunan pada akhir kebuntingan sampai dua minggu setelah melahirkan. Pada kelompok Zn 60 ppm memperlihatkan konsentrasi IgG lebih tinggi dibanding kelompok Zn 80 ppm.
Konsentrasi Protein Total, Albumin, dan Globulin Anak Kambing Peranakan Etawah Setelah Pemberian Berbagai Sediaan Kolostrum* (TOTAL PROTEIN, ALBUMIN, AND GLOBULIN CONCENTRATIONS ON ETTAWAH CROSSBREED NEONATES FOLLOWING THE ADMINISTRATION OF VARIOUS FORM O Anita Esfandiari; Sus Derthi Widhyari; Setyo Widodo; I Wayan Teguh Wibawan; Dondin Sajuthi; I Ketut Sutama
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (140.331 KB)

Abstract

This experiment was conducted to study the profile of total protein, albumin, and globulin concentrationson Ettawah crossbreed neonates after consuming various colostrums. Twenty four healthy neonatal kidswere used in this study. The neonates were divided into four groups. Each group received fresh maternal(goat) colostrum, frozen-thawed bovine colostrum, bovine spray dried colostrum, and bovine powdercommercial colostrum, respectively. Colostrums were given at 10% of body weight directly after birth andfollowed by the same amount every 12 hours, for three days. The blood was taken from jugular vein at 0, 12,24, 48, 72, and 168 hours after birth to determine total protein, albumin, and globulin concentrations.Results of this study indicated that the serum total protein and globulin concentration increased andreached the peak at 24 hours after birth. Compared to the concentration at birth, the increase of totalprotein concentration were 62.77%, 59.26%, 48.05%, and 66.67% in fresh maternal (goat), frozen-thawedbovine, bovine spray dried, and commercial bovine colostrum, respectively. Serum globulin concentrationincreased 4.9, 4.4, 4.8, and 14.6 times in fresh matermnal goat, frozen-thawed bovine, spray dried, andcommercial bovine colostrums respectively, compared to the concentration at birth. In conclusion, theconsumption of various colostrums i.e. fresh maternal goat colostrums, bovine colostrums (frozen-thawed,spray dried and commercial colostrums) would increase the concentration of blood total protein and globulin,which both reached the highest concentration at 24 h after birth.
Anestesi Infus Gravimetrik Ketamin dan Propofol pada Anjing (THE GRAVIMETRIC INFUSION ANAESTHESIA WITH KETAMINE AND PROPOFOL IN DOGS) I Gusti Ngurah Sudisma; Setyo Widodo; Dondin Sajuthi; Harry Soehartono
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.965 KB)

Abstract

This study aim was to evaluate quality of anaesthesia by using gravimetric infusion anaesthesia withketamine and propofol in dogs. The quality of anaesthesia, duration of actions, and the physiological responsseof anaesthesia were evaluated in twenty domestic dogs. Anaesthesia was induced intramuscularly withatropine (0.03 mg/kg)-xylazine (2 mg/kg) (AX), intravenously ketamine-propofol (KP) (4 mg/kg), andmaintained with continuous intravenous infusion with pre-mixed propofol (P) and normal saline containing2 mg/ml of propofol and 2 mg/ml of ketamine (K). Domestic stray dogs were randomly divided into fivegroups. Groups AXKP-K2P2, AXKP-K4P4, and AXKP-K6P6 were treated with ketamine-propofol the dose0.2 mg/kg/minute, 0.4 and 0.6 mg/kg/minute respectively, while group AXKP-P4 was given propofol 0.4 mg/kg/minute and group AXKP-I was given isoflurane 1-2%. Heart rate (HR), respiratory rate (RR),electrocardiogram (ECG), blood oxygen saturation (SpO2), end tidal CO2 (ET CO2), and capillary refill time(CRT) were measured. No significant difference (P>0.05) found between the groups in anaesthetion times.All groups showed rapid and smooth inductions, prolonged surgical stage, and rapid recovery. Groups AXKPK2P2and AXKP-K4P4 showed minimal physiological effect on the dogs. The HR, RR, ET CO2, SpO2, CRT,and ECG wave were stabl. Combination of AXKP-K6P6 induced SpO2 depression, increased and instabilityof HR, RR and ET CO2. Groups AXKP-P4 showed decreased of HR and respiratory depression. All anaestheticcombinations showed no significant influence (P>0.05) on the electricity of the dog’s heart. The combinationof ketamine-propofol at dose 0.2 and 0.4 mg/kg/minute were found to be better as an application formaintaining anaesthesia by gravimetric continuous intravenous infusion. The method is a suitablealternative for inhalation anaesthesia in dogs.
Profil Kadar Kortisol dan Seng pada Kambing Peranakan Etawah Saat Melahirkan yang Diberi Tambahan Seng dalam Pakannya Sus Derthi Widhyari; Setyo Widodo; I Wayan Teguh Wibawan; I Ketut Sutama; Anita Esfandiari
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (170.536 KB)

Abstract

The objective at this experiment was to study the role of zinc supplementation (Zn) in minimizingstress at parturition. Thirty etawah cross breed age 3-6 years with average body weight 30-50 kg were usedin this study and divided into three groups ; i) animal were given grass + feed concentrate + ZnSO4 40 mg/kg dry matter ( control group) ; (ii) grass + feed concentrate + ZnSO4 60 mg/kg dry matter (treatment 1), and(iii) grass + feed concentrate + ZnSO4 80 mg/kg dry matter (treatment 2), respectively drinking water weregiven ad libitum. To monitor zinc and cortisone level, blood samples were withdrawn from each animalevery two weeks starting at age of pregnancy 3 months up to two months post parturition, in addition, tomonitor the cortisone level blood samples were also collected at 1,2,3 and 7 days post parturition. The Znand cortisone serum were analyzed using atomic absorbent spectro photometric (AAS) andradioimmunoassay (RIA), respectively the result showed that generally pregnancy at the beginning andget serum level at cortisone was relatively similar between the three animal groups and there was atendency to increase and reached peak at parts then gradually decrease until the and of the observation. Atparturition the lowest serum level at cortisone (19.00±18.72 mg/ml) was observed in animals receiving the60/ dry matter zinc supplement compared to animal receiving 80 mg/ dry matter zinc ( 52.65 ± 30.83 mg/ml) and control animal ( 75.92 ± 42.88 mg/ml). in addition serum level at Zn was significantly higher inanimal receiving 60 mg and 80 mg Zn in their diets compared to control animals. The best profiles wereseen in animal given 60 mg/kg dry matter Zn supplement. In conclusion, the addition of Zn in the diets hadsignificant effect in minimizing stress as pictured by the low serum level at cortisone at parturition.
Co-Authors Agatha W. Sanjaya Agus Lelana Agus Wijaya Agus Wijaya Anita Esfandiari Chusnul Choliq Deny Setyo Wibowo Deskiharto, Arman Dewi, Fitriya Nur Annisa Dondin Sajuthi Effendi, Siti Aisyah Rahmalia Eko Sugeng Pribadi Erni H Purwaningsih Eva Harlina Heinrich Enbergs I Gusti Ngurah Sudisma I Ketut Sutama I W.T. Wibawan I wayan Teguh Wibawan IETJE WIENTARSIH Intan Maria Paramita Kadri, Febrina Suci Dwi Kartjito, Nicolas Edward Christanto Leni Maylina M Hanifuddin M. Hanifuddin Madyastuti, Rini Milda Fibre MIRNAWATI SUDARWANTO Muhammad Hanifuddin Retno Wulansari Rizal Rahadian Ramdhany Rona Malam K Rona Malam Karina Sherli Noviaria Pakpahan Sherly Noviaria Pakpahan Solinda, Gamelita Rizkawandi Sus Derthi Widhyai Sus Derthi Widhyari TATI NURHAYATI Wijayanti, Revi Esti