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Cytotoxic of Usnic Acid Isolated from Ramalina sp.: Cytotoxic of Usnic Acid Isolated from Ramalina sp. Darmawan, Akhmad; Maulidiyah; Megawati; Ariani, Novita; Aisya, Sitti; Sukirno; Randy, Ahmad; Primahana, Gian; Hendra, Medi; Nurdin, Muhammad
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.14

Ramalina sp. (Ramalinaceae) is a type of lichen known to contain many active secondary metabolite compounds that have the potential to be used as medicine or medicinal raw materials. One of the biological activities possessed by Ramalina sp. lichen is its anticancer activity. This research aims to isolate and identify active secondary metabolite compounds from the methanol extract of the Ramalina sp. lichen and to find out the cytotoxic activity of the isolated compound against MCF7 breast cancer cells. Compound 1 (usnic acid) was successfully isolated from fraction A. The isolation and purification process was carried out starting with a maceration process using acetone solvent, followed by silica gel column chromatography using a gradient solvent system consisting of n-hexane, n-hexane:EtOAc, EtOAc, EtOAc:MeOH, and MeOH with 5% increment of polarity to obtain 17 fractions (F-1 to F-17). From the 17 fractions obtained, fraction 3 (F-3) and fraction 4 (F-4) (eluted with n-hexane:EtOAc 30%), which had the same TLC profile, were combined and named as fraction A. Compound 1 (50 mg) is a yellow needle crystal that was formed in a bottle of fraction A, which was obtained after the process of combining fractions F-3 and F-4 and solvent evaporation process. The crystals were then separated and purified with CHCl3 and MeOH. Compound 1 was then characterized based on spectroscopic data. Various spectroscopic analysis data, including FTIR, 1D- and 2D-NMR, and LC-ESI-MS, show that Compound 1 is a dibenzofuran derivative compound with 18 carbons (3 from carbonyl groups (C=O) and 3 from methyl groups) and 2 hydroxyl (-OH). Cytotoxicity assay showed that at a low concentration of 18.75 ug/mL, Compound 1 caused a 67.06% decrease in MCF7 viability

Pengaruh Melakukan Istinsyaq Terhadap Derajat Sumbatan Hidung Pada Penderita Rinitis Alergi Irfandy, Dolly; Ariani, Novita; Fariz, Kamal
Oto Rhino Laryngologica Indonesiana Vol. 53 No. 1 (2023): VOLUME 53, NO. 1 JANUARY - JUNE 2023
Publisher : PERHATI-KL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32637/orli.v53i1.554

AbstrakLatar Belakang: Rinitis alergi adalah inflamasi mukosa hidung yang menyerang 30% populasi dunia. Sebuah penelitian menunjukkan bahwa istinsyaq dapat mengurangi derajat sumbatan hidung. Objektif: Penelitian ini bertujuan untuk mengetahui pengaruh melakukan istinsyaq dalam wudhu terhadap derajat sumbatan hidung pada penderita rinitis alergi.Metode: Jenis penelitian ini adalah pre-eksperimental dengan desain one group pretest-posttest. Populasi adalah siswa kelas X dan XI di SMA IT Imam Syafi’I Kota Batam tahun ajaran 2020/2021 yang menderita rinitis alergi dan bersedia untuk berpartisipasi. Skrining rinitis alergi menggunakan kuesioner SFAR dan derajat sumbatan hidung dinilai dengan menggunakan kuesioner NOSE.Hasil: Didapatkan sampel berjumlah 24 siswa. Rata–rata total skor NOSE sebelum edukasi perlakuan istinsyaq adalah 30 ± 18, dan setelah perlakuan istinsyaq di hari ke-14 menurun menjadi 21 ± 18 dan di hari ke-28 menurun kembali menjadi 14 ± 16. Didapatkan penurunan bermakna rata–rata total skor NOSE dengan menggunakan uji Wilcoxon yaitu nilai p value (0,019) < 0,05 di hari ke-14 dan nilai p value (0,002) < 0,05 di hari ke-28 setelah perlakuan istinsyaq.Kesimpulan: Istinsyaq dapat menurunkan rata–rata total skor kuesioner NOSE pada siswa dengan rinitis alergi. Dengan demikian, istinsyaq efektif untuk mengurangi derajat sumbatan hidung pada penderita rinitis alergi.Kata kunci: Istinsyaq, Rinitis Alergi, Score For Allergic Rhinitis, Nasal Obstruction Symptom Evaluation AbstractBackground: Allergic rhinitis is an inflammation of the nasal mucosa that affects 30% of the world's population. A study showed that istinsyaq can reduce the degree of nasal obstruction.Objective: This study aims to determine the effect of performing istinsyaq in wudhu on the degree of nasal obstruction in patients with allergic rhinitis.Methods: This study is a pre-experimental with one group pretest-posttest design. The population is class X and XI students at SMA IT Imam Syafi'I Batam City for the 2020/2021 academic year who suffer from allergic rhinitis and are willing to participate. Allergic rhinitis was screened using the SFAR questionnaire and the degree of nasal obstruction was assessed using the NOSE questionnaire.Results: The sample obtained was 24 students. The average total NOSE score before istinsyaq treatment education was 30 ± 18, and after istinsyaq treatment on the 14th day it decreased to 21 ± 18 and on the 28th day it decreased again to 14 ± 16. There was a significant decrease in the average total NOSE score using the Wilcoxon test, namely the p value (0.019) < 0.05 on the 14th day and p value (0.002) < 0.05 on the 28th day after istinsyaq treatment.Conclusion: In conclusion, istinsyaq can reduce the average total score of the NOSE questionnaire in students with allergic rhinitis. Thus, istinsyaq is effective in reducing the degree of nasal obstruction in patients with allergic rhinitis.Keyword: Istinsyaq, Allergic Rhinitis, Score For Allergic Rhinitis, Nasal Obstruction Symptom Evaluation

Profil Pasien Uveitis di Plokilinik Mata RSUP Dr. M. Djamil Padang Pada Tahun 2020-2022 Helery, Muhammad Arsyan; Vitresia, Havriza; Ariani, Novita; Hidayat, Muhammad; Semiarty, Rima; Arfiani Rusjdi, Dina
Jurnal Ilmu Kesehatan Indonesia Vol. 6 No. 1 (2025): Maret 2025
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jikesi.v6i1.1337

Uveitis is an inflammation of the middle layer of the eye, the uvea, which consists of the iris, ciliary body and choroid. Uveitis is one of the causes of ocular morbidity. Common symptoms of uveitis include red eye, eye pain and blurred vision. Patients with uveitis can experience decreased vision to blindness. Research on uveitis in Indonesia is still limited. This study aims to determine the profile of uveitis patients at the ophtalmology departement of RSUP Dr. M. Djamil Padang. This study used a descriptive research design with a retrospective approach which was carried out by observing data from the medical records of uveitis patients at the ophtalmology departement and the medical records section of RSUP DR. M. Djamil Padang period 2020-2022. The study used a total sampling technique. Patient data were analyzed univariately and data presentation in the form of frequency distribution tables. In this study, a total of 50 samples were obtained. The results of this study found out that uveitis patients mostly female (52%) and the majority in the age range 20-60 years (90%). The most common types of uveitis based on anatomical location are panuveitis (56%) and unilateral lateralization (62%). The main etiology of uveitis was infection (42%). Most of the uveitis patients had clinical manifestation of blurred vision (72%) and red eye (72%). More than half uveitis patients visus was <3/60, categorized as blindness (52%).

FAKTOR-FAKTOR YANG MEMPENGARUHI SURVIVAL RATES PASIEN YANG MENDAPATKAN RADIOTERAPI PADA KANKER OVARIUM : SYSTEMATIC REVIEW Genia, Dara Indah; Ariani, Novita; Suharti, Netti; Syamel, Muhammad; Elliyanti, Aisyah; Intan, Shinta Ayu
EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan Vol. 2 No. 2 (2025): EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan, Juni 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/empiris.v2i2.1266

Ovarian cancer is known as a silent but deadly disease (silent killer) so it is not uncommon for sufferers to end in death. Radiotherapy for ovarian cancer is indicated as adjuvant therapy for advanced stages in an effort to extend survival rate. This review aims to determine the factors that influence survival rates, local control, and toxicity leves in patients receiving radiotherapy for ovarian cancer. This research is a narrative reviw using the PRISMA method. A literature search was conducted in three databases Pubmed, CENTRAL and Science Direct in the 2013-2023 period. The specified keywords are adapted to the terms Medical Subject Headings (MeSH) and research questions using the PEO formula. Articles were selected based on predetermined inclusion and exclusion criteria. There are 9 selected primary articles and included in the narrative reflection. As many as 3 out of 9 studies stated first-line therapy in the form of total debulking surgery of lesions treated before RT as a factor that significantly influenced survival rates, 2 studies stated CA-125 levels before and after RT had a significant effect on survival rates, 2 studies explained that patients with platinum sensitivity and CCC histological subtypes had better survival rates, and 2 other literatures explained that cancer stage at diagnosis, cancer volume, and post-treatment or not after RT also significantly affected survival rates. Ovarian cancer patients who undergo radiotherapy have better survival rate than patients who do not receive radiotherapy with excellent local control and limited radiation related toxicity that is relatively well tolerated by patients

Pengaruh Pemberian Blastocystis sp. Terhadap Gambaran Histopatologi Usus Tikus Berdasarkan Modifikasi Kriteria Barthel Manja Aqilah, Giffary Zahida; Nofita, Eka; Ariani, Novita; Renita Rusjdi, Selfi; Tofrizal, Tofrizal; Abdiana, Abdiana
Jurnal Ilmu Kesehatan Indonesia Vol. 6 No. 2 (2025): Juni 2025
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jikesi.v6i2.1096

Latar Belakang: Blastocystis sp. adalah parasit protozoa usus ditemukan di saluran usus manusia dan hewan. Gambaran terkait Blastocystis sp. seperti mual, anoreksia, diare, dan dikaitkan dengan kejadian Inflammatory Bowel Disease dan Irritable Bowel Syndrome. Patogenitas Blastocystis sp. masih kontravesi. Tujuan dari penelitian ini untuk melihat reaksi inflamasi di usus (sekum dan kolon) yang ditimbulkan Blastoystis sp. melalui perubahan histopatologi berdasarkan modifikasi kriteria Barthel Manja. Objektif: Penelitian ini bertujuan untuk melihat reaksi inflamasi di usus (sekum dan kolon) yang ditimbulkan Blastocystis sp. melalui perubahan histopatologi berdasarkan modifikasi kriteria Barthel Manja. Metode: Jenis penelitian ini adalah penelitian true eksperimental dengan rancangan posttest-only control design. Sampel penelitian adalah 21 tikus galur Wistar jantan, yang dibagi menjadi 3 kelompok (PI, PII, dan K). Kelompok PI diinokulasikan oral dosis 105. Kelompok PII diinokulasikan oral Blastocystis sp. dosis 104. Kelompok K diberikan normal saline tanpa pemberian Blastocystis sp. Hasil Penelitian: Hasil penelitian menunjukkan terdapat pengaruh pemberian Blastocystis sp. terhadap edema submukosa, infiltrasi sel PMN, dan kerusakan epitel di kolon. Tidak berpengaruh terhadap hiperplasia sel goblet di kolon dan sekum. Skor akhir menunjukkan Blastocystis sp. tidak berpengaruh terhadap inflamasi usus berdasarkan modifikasi kriteria Barthel Manja. Kesimpulan: Pemberian Blastocystis sp. hanya berpengaruh terhadap edema submukosa, infiltrasi sel PMN, dan kerusakan epitel di kolon.

Cytotoxic of Usnic Acid Isolated from Ramalina sp.: Cytotoxic of Usnic Acid Isolated from Ramalina sp. Darmawan, Akhmad; Maulidiyah; Megawati; Ariani, Novita; Aisya, Sitti; Sukirno; Randy, Ahmad; Primahana, Gian; Hendra, Medi; Nurdin, Muhammad
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.14

Ramalina sp. (Ramalinaceae) is a type of lichen known to contain many active secondary metabolite compounds that have the potential to be used as medicine or medicinal raw materials. One of the biological activities possessed by Ramalina sp. lichen is its anticancer activity. This research aims to isolate and identify active secondary metabolite compounds from the methanol extract of the Ramalina sp. lichen and to find out the cytotoxic activity of the isolated compound against MCF7 breast cancer cells. Compound 1 (usnic acid) was successfully isolated from fraction A. The isolation and purification process was carried out starting with a maceration process using acetone solvent, followed by silica gel column chromatography using a gradient solvent system consisting of n-hexane, n-hexane:EtOAc, EtOAc, EtOAc:MeOH, and MeOH with 5% increment of polarity to obtain 17 fractions (F-1 to F-17). From the 17 fractions obtained, fraction 3 (F-3) and fraction 4 (F-4) (eluted with n-hexane:EtOAc 30%), which had the same TLC profile, were combined and named as fraction A. Compound 1 (50 mg) is a yellow needle crystal that was formed in a bottle of fraction A, which was obtained after the process of combining fractions F-3 and F-4 and solvent evaporation process. The crystals were then separated and purified with CHCl3 and MeOH. Compound 1 was then characterized based on spectroscopic data. Various spectroscopic analysis data, including FTIR, 1D- and 2D-NMR, and LC-ESI-MS, show that Compound 1 is a dibenzofuran derivative compound with 18 carbons (3 from carbonyl groups (C=O) and 3 from methyl groups) and 2 hydroxyl (-OH). Cytotoxicity assay showed that at a low concentration of 18.75 ug/mL, Compound 1 caused a 67.06% decrease in MCF7 viability

ISOLATION AND IDENTIFICATION OF APIGENIN, A FLAVONOID COMPOUND FROM MACARANGA HYPOLEUCA (REICHB.F. & ZOLL.) Aisya, Sitti; Megawati, Megawati; Ariani, Novita; Sukirno, Sukirno; Minarti, Minarti; Kurniawan, Hendris Hendarsyah; Hidayat, Arief; Hendra, Medi; Primahana, Gian; Darmawan, Akhmad
Berita Biologi Vol 23 No 1 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2024.3242

The study on Macarang hypolueca (Reichb.f. & Zoll.), which was collected from secondary forests around Samarinda City, East Kalimantan, involved phytochemical investigations that led to the isolation of a flavone type compound of flavonoid from the ethyl acetate fraction. To separate the compounds, silica gel column chromatography was utilized with a gradient solvent system of n-hexane and ethyl acetate, along with the addition of 5%. Infrared analysis (FTIR), mass spectrum (LC-ESI-MS), and nuclear magnetic resonance (1D- and 2D-NMR) were used to identify and elucidate the structure. Based on spectroscopic data and comparison with appropriate references, the isolated compound was identified as apigenin

Evaluasi Penggunaan Obat Antiemetik pada Pasien Kanker Ovarium Pasca Kemoterapi Putra, Xavier Novdi Tansyah; Rustam, Erlina; Ariani, Novita; Rahmatini; Muhammad, Syamel; Intan, Shinta Ayu
Jurnal Ilmu Kesehatan Indonesia Vol. 6 No. 4 (2025): Desember 2025
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jikesi.v6i4.1362

Latar Belakang: Kanker ovarium merupakan kanker pada perempuan yang cukup sering terjadi dan mematikan. Penggunaan kemoterapi dalam pengobatan dapat menyebabkan mual muntah sehingga diperlukan penggunaan obat yang efektif dan rasional. Objektif: Untuk mengevaluasi penggunaan obat antiemetik pada pasien kanker ovarium pasca kemoterapi di RSUP Dr. M. Djamil Padang tahun 2020-2022. Metode: Penelitian ini merupakan penelitian deskriptif dengan pendekatan retrospektif. Data rekam medis dikumpulkan dengan teknik total sampling. Sampel yang memenuhi inklusi berjumlah 45 sampel. Hasil: Hasil penelitian didapatkan karakteristik terbanyak pada kelompok usia 41-60 tahun (48,9%), berpendidikan SLTU (75,6%), dan pekerjaan ibu rumah tangga (55,6%). Jenis obat kemoterapi yang paling banyak digunakan adalah paklitaksel-karboplatin (42,2%). Sebagian besar pasien mendapatkan antiemetik pra-kemoterapi berupa deksametason-difenhidramin-ondansetron-ranitidin (71,1%) serta pasca kemoterapi berupa metoklopramid-ranitidin dalam 3 siklus (74,8%). Rasionalitas penggunaan obat antiemetik pada pasien kanker ovarium pasca kemoterapi yaitu kesesuaian indikasi 93,3%, kesesuaian obat 62,2%, kesesuaian dosis 91,1%, kesesuaian pasien 97,8% dan potensi interaksi obat 2,2%. Kesimpulan: Karakteristik pasien kanker ovarium di RSUP Dr. M. Djamil Padang tahun 2020-2022 sering terjadi pada kelompok usia 40-60 tahun, pendidikan SLTU, dan pekerjaan ibu rumah tangga. Sebagian besar pasien mendapatkan obat kemoterapi paklitaksel-karboplatin dan obat antiemetik pra-kemoterapi deksametason-domperidon-ondansetron-ranitidin serta pasca kemoterapi metoklopramid-ranitidin. Rasionalitas penggunaan obat antiemetik pada pasien kanker ovarium pasca kemoterapi sebagian besar sudah sesuai. Kata kunci: antiemetik, kanker ovarium, kemoterapi, rasionalitas

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