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All Journal Pharmascience Jurnal Sehat Mandiri INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Best Journal (Biology Education, Sains and Technology) Journal of Pharmaceutical and Health Research Jurnal EduHealth Jurnal Pijar MIPA Jurnal Matematika Dan Ilmu Pengetahuan Alam LLDikti Wilayah 1 (JUMPA) Jurnal Kedokteran Hewan PharmaCine : Journal of Pharmacy, Medical and Health Science
Mierza, Vriezka
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Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary

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Molecular identification and antibacterial activity of endophytic bacteria from Bambusa vulgaris leaves as antibacterial potential against phatogenic microoganism Malau, Jekmal; Mierza, Vriezka; Mulki, Munir Alinu; Urbaningrum , Lestari Mahardika; Hasna, Vina Luthfiana; Debora, Priscinya Christiana
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 2 (2025): From Natural Compounds to Disease Mechanisms: An Integrated Research Outlook
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i2.6538

Abstract

The increasing prevalence of antibiotic-resistant bacteria poses a critical global health concern, necessitating the exploration of novel antibacterial solutions. Endophytic bacteria, which colonize plant tissues without causing harm, have gained attention as potential sources of bioactive metabolites. This study aimed to isolate and characterize endophytic bacteria from Bambusa vulgaris leaves and evaluate their antibacterial potential against pathogenic microorganisms. Leaf samples were collected from Bekasi, West Java, and subjected to a surface sterilization process prior to bacterial isolation. A total of 12 bacterial strains were successfully obtained and screened for antibacterial activity against Bacillus subtilis, Propionibacterium acnes, Pseudomonas aeruginosa, and Staphylococcus epidermidis using the agar well diffusion assay. Three isolates exhibited notable inhibitory activity, with P8 demonstrating the strongest antibacterial effects against B. subtilis, P. acnes, and S. epidermidis. The two most potent isolates, P8 and K3, were characterized via 16S rRNA gene sequencing. Genomic DNA extraction was performed, followed by Polymerase Chain Reaction (PCR) amplification using the universal primers 27F (5′-AGAGTTTGATYMTGGCTCAG-3′) and 1492R (5′-GGTTACCTTGTTACGACTT-3′). Sequencing and The Basic Local Alignment Search Tool (BLAST) analysis confirmed that isolate P8 exhibited 100% similarity to B. subtilis strain LZH-H1, whereas isolate K3 shared 99.85% similarity with Pantoea stewartii subsp. indologenes strain SR2-12. These findings suggest that endophytic bacteria from B. vulgaris endohytic bacteria hold promise as potential sources of antibacterial compounds. Further research is necessary to purify and characterize these bioactive metabolites for potential pharmaceutical applications.
Literature Review: Standardization of Rutin Compounds in Several Plant Types Mierza, Vriezka; Odhia, Febry Nola; Tyasna, Putri Setya; Nisa, Dila Qhoirul; Andriani, Nadia
Jurnal EduHealth Vol. 13 No. 02 (2022): Jurnal eduHealth, Periode Oktober - December, 2022
Publisher : Sean Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.87 KB)

Abstract

Rutin is a compound derived from flavonoids. Rutin is a quercetin glycoside with a disaccharide consisting of glucose and rhamnose. Rutin is known to have activity as an antioxidant, anti-radical, estrogenic, anti-inflammatory, antiviral, antitumor, cytotoxic and protects against organ damage. The manufacture of herbal medicines must first go through a standardization process. This review was conducted to identify the rutin contained in an extract by means of Visible Light, KLT, HPLC, and LCMS. This research uses the literature review method by collecting and identifying relevant research data sources or articles published online, through the Google Scholar web database and Science Direct.  
EFFECT OF HESPERETIN TREATMENT ON BLOOD GLUCOSE LEVEL, SPERMATOZOA QUALITY, AND SPERMATOZOA QUANTITY IN ALLOXAN-INDUCED DIABETIC MICE Nerdy, Nerdy; Meliala, Linta; Barus, Bunga Rimta; Lestari, Puji; Ginting, Selamat; Ariani, Peny; Mierza, Vriezka; Bakri, Tedy Kurniawan
Jurnal Kedokteran Hewan Vol 15, No 1 (2021): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i1.18406

Abstract

Male infertility has occurred rapidly in the last few decades, primarily in developing countries. An antioxidant, hesperetin is a flavonoid that is found in abundance in orange peels. The aims of this research were to determine the effect of hesperetin on blood sugar levels, spermatozoaquality, and spermatozoa quantity. The research structure included induction of diabetes mellitus and treatment for 8 weeks, followed bydetermination of blood sugar levels, spermatozoa quality, and spermatozoa quantity. Hesperetin has the ability to restore blood sugar levels, spermatozoa quantity, seminiferous tubules diameter, and testicular weight, volume, and germinal epithelial layer thickness with significant difference from the normal control group. Hesperetin did not fully restore spermatozoa motility, viability, and morphology with significant difference from the normal control group, nor from the positive control group. However, overall, hesperetin decreased blood glucose levels, increased spermatozoa quantity, and improved the spermatozoa quality in alloxan-induced diabetes mellitus mice. Dose-dependent activity was observed with the optimum dose at 200 mg/kg body weight.
Co-Authors Advaita, Chalisya Vanya Aisiyah, Adella Ananda, Meisya Dwi Andri, Ihsan Alfi Andriani, Nadia Apriani, Risa Dwi Ardhita, Intan Ariani, Peny Arsylrakhmatika, Giga Anugrah BAKRI, TEDY KURNIAWAN Barus, Bunga Rimta Debora, Priscinya Christiana GINTING, SELAMAT Hasna, Vina Luthfiana Irwansyah, Silvana Lestari Iskandar, Putri Mutiara Jekmal Malau Khaerunisa, Aulia Khuriyah . Meliala, Linta Muhammad Hanafi Munir Alinu Mulki Nailuvar, Regita Nerdy, Nerdy Nilsya Febrika Zebua, Nilsya Febrika Nisa, Dila Qhoirul Odhia, Febry Nola Pandiangan, Martina Panjaitan, M Ridho Pangestu PUJI LESTARI Razali, Mariany Sakinah, Irene Virda Saputri, Muharni Septimar, Elviza Sudarjat, Hadi Tyasna, Putri Setya Urbaningrum , Lestari Mahardika Yessi Febriani