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All Journal Indonesian Journal of Chemistry Jurnal Biomedika dan Kesehatan
Yousif, Emad
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Enhanced UV Resistance of Poly(methyl methacrylate) Through Schiff Base and Metal Oxide Nanoparticle Incorporation Hussein, Ahmed; Yousif, Emad; Rasheed, Malath; Ahmed, Dina; Bufaroosha, Muna; Kadhom, Mohammed
Indonesian Journal of Chemistry Vol 25, No 2 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.97173

Abstract

In this work, a novel poly(methyl methacrylate) (PMMA) material was prepared by incorporating Schiff base and metal oxide nanoparticles (NPs). Hence, different polymer derivatives were produced, and their resistance to light degradation was improved. PMMA was subjected to chemical modification, first by reacting PMMA with an excess of ethylene diamine to reduce the occurrence of cross-linking. Second, it underwent a reaction with the amino group of 4-methoxybenzaldehyde to produce Schiff base molecules. These molecules were subsequently infused with various metal oxide NPs, namely: TiO2, CuO, Cr2O3, Co2O3, and NiO to work as photostabilizers and prepare modified PMMA films. The synthesized polymers were analyzed using Fourier transform infrared (FTIR) and NMR spectroscopy to confirm their structures and determine the extent of structural alterations. FTIR and weight loss measurements assessed modified PMMA's UV stabilization. The effectiveness of functional groups was assessed by monitoring their growth. Additional analyses were done included atomic force microscopy (AFM), scanning electron microscopy (SEM), and microscopic imaging. Unmodified PMMA performed worse than Schiff base polymers. The modified PMMA photodegraded less than the blank films after 300 h of UV exposure. The unit's strong internal conjugation absorbs UV light, which improves performance.
Deteksi gen secara Epidemiologi Molekuler pada Isolat Klinik Klebsiella pneumoniae dari Pasien Dewasa dengan komorbid di Rumah Sakit Baghdad Kudaer, Nuha B; Risan, Mohsen Hashim; Raheem, Rasha; Zainulabdeen, Khalid; Salman, Israa; Hairunisa, Nany; Amalia, Husnun; Hameed, Seenar; Yousif, Emad
Jurnal Biomedika dan Kesehatan Vol 6 No 2 (2023)
Publisher : Fakultas Kedokteran Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/JBiomedKes.2023.v6.196-215

Abstract

Background Klebsiella species is one of the most worldwide opportunistic pathogens in the world that cause infections at multiple sites like lung, urinary tract, bloodstream, wound or surgical site, and brain. Klebsiella pneumoniae has many types of virulence factors that give the bacteria the ability to invade the host and cause infections. Methods A total of (105) human clinical samples were collected from different patients with different cases. Klebsiella pneumonia were isolated from 30 samples. The bacteria were identified by biochemical tests and certified by using VITEK 2 system and genetically by amplify using PCR for 16 rRNA gene. One of the Klebsiella pneumoniae virulence factors is the capsule that is responsible for the macrophage resistance and its also responsible for serotype. So to confirm the strains isolated, PCR Gene detection of, MagA, K2A, RmpA, and Kfu, where (magA, k2A, rmpA, kfu) gens are responsible for capsule synthesis of Klebsiella pneumonia have been done. Results There were 30 strains of Klebsiella pneumoniae were identified. Epidemiological studies show that infections are preceded by gastrointestinal colonization, with the gastrointestinal tract being the most important reservoir for transmission. The primary bacterial isolates were identified based on their cultural, microscopic, and biochemical traits. The API system (API 20E) and VITEK 2 systems were used to confirm the identity of the bacterial isolates. Colony morphology revealed that Klebsiella pneumonia was Gram-negative, non-motile, microscopic straight rods grouped singly or in pairs. Biochemical tests showed that neutral end products prevailed over acidic end products, with Klebsiella producing negative results for indole. The Kligler Iron Agar (KIA) test showed that Klebsiella isolates changed the color of the slant and butt, producing an acidic slant and acid butt, along with gas production. The urease test for Klebsiella distinguishes between Enterobacter and Klebsiella isolates, as Klebsiella can manufacture the urease enzyme. The indole test distinguishes K. pneumonia from K. pneumonia from K. mobiliz. The API 20E system was used for biochemical testing, and the VITEK 2 system was used for identification. The VITEK 2 system was used to test for biochemical and antibiotic susceptibility, and the results supported the findings from morphological and biochemical analyses. PCR amplification was used for molecular detection, and all nine isolates tested positive for the magA and k2A genes. The ferric iron uptake system gene (kfu) and the extracapsular polysaccharide synthesis regulator gene (rmpA) were amplified using specific primer pairs. The VITEK 2 system demonstrated promising outcomes for K. pneumoniae identification. However, standard diagnostic methods are costly and time-consuming. Conclusions Navigating the complex landscape of infectious diseases, collaborative research projects have become indispensable in unraveling the mysteries surrounding pathogens. The project in Baghdad, having stood as a shining example of such collaboration, provided valuable insights into the identification, characterization, and molecular aspects of Klebsiella pneumoniae strains. The findings, have not only contributed to the scientific community's understanding of bacterial infections but also paved the way for enhanced strategies in diagnosis and treatment.
Studi Faktor-Faktor yang Berhubungan dengan HIV pada Perempuan di Kalimantan Barat, Indonesia Hairunisa, Nany; Sabrina, Isra; Amalia, Husnun; Mashabi, Yasmine; Zaina, Nashita Amira; Yousif, Emad
Jurnal Biomedika dan Kesehatan Vol 6 No 1 (2023)
Publisher : Fakultas Kedokteran Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/JBiomedKes.2023.v6.89-107

Abstract

Background According to national data, West Kalimantan is one of the ten top provinces that reported AIDS cases, ranked eighth. Until December 2019, based on data recorded in the Provincial Health Office that reported to the Indonesia Ministry of Health, the AIDS case rate in West Kalimantan was 49.35, showing over the national AIDS case rate (38.93). In this study, an unmatched case-control determines the factors associated with HIV/AIDS among women in West Kalimantan, Indonesia. Methods The study was conducted among 90 women who tested positive for HIV/AIDS (cases) and 90 who tested negative for HIV/AIDS (controls). Data were collected using questionnaires from June 2019 to August 2019 at three community health centres in West Kalimantan. Data were analyzed using IBM Statistical Package Social Science (SPSS) 19. Descriptive analyses and Multivariate logistic regression analysis were used to examine the factors associated with HIV/AIDS. Results: This study has found a significant association between crucial aspects such as marital status, occupation, knowledge about HIV/AIDS, HIV treatment, and VCT (Voluntary Counseling and Testing) service program with cases of HIV/AIDS. A significant association between marital statuses with HIV/AIDS suggests that divorced/widowed have a higher risk of having HIV/AIDS, followed by the married group. Women with good educational backgrounds will likely know how to prevent HIV infection and take preventive measures to protect themselves. Results This study found a significant relationship between several important aspects such as marital status, occupation, knowledge of HIV/AIDS, HIV treatment, and the VCT (Voluntary Counseling and Testing) service program with HIV/AIDS cases. The significant relationship between marital status and HIV/AIDS shows that divorced/widows have a higher risk of contracting HIV/AIDS, followed by the married group. Women with a good educational background tend to know about HIV infection prevention measures and take precautions to protect themselves. Conclusions Some factors are significantly related to HIV/AIDS. Thus, educating women about sex could potentially decrease the number of HIV/AIDS cases.
Studi Banding tentang pengembangan vaksin untuk COVID-19 Alsayed, Raghda; Zainulabdeen, Khalid; Salman, Israa; Hairunisa, Nany; Amalia, Husnun; Yusop, Rahimi M; Redwan, Amamer M; Yousif, Emad
Jurnal Biomedika dan Kesehatan Vol 6 No 1 (2023)
Publisher : Fakultas Kedokteran Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/JBiomedKes.2023.v6.127-132

Abstract

The human respiratory system can be infected with coronaviruses (CoVs), one of the families of viruses that can lead to symptoms ranging from mild to fatal. Seven genera of CVS have been identified as having human infection to date. The virus can spread as fine, liquid particles from the mouth or nose of an infected person when coughing, sneezing, speaking, or breathing. The diameter of droplet particles ranges from large to small. In this review, we summarize vaccine development for COVID-19. We anticipate this study will be a valuable resource for any researcher interested in creating a COVID-19 vaccine.
Emerging Threats in the Age of Pandemics: A Focus on COVID-19 and the Novel Sub-Variant EG 5 ("Eris"): Review Article Alsayed, Raghda; Thamer, Hamsa; Hameed, Seenar; Kadhom, Mohammed; Hairunisa, Nany; Amalia, Husnun; Mashabi, Yasmine; Ahmed, Dina; Mahdi, Sarah; Husain, Amani; Salman, Israa; Yousif, Emad
Jurnal Biomedika dan Kesehatan Vol 7 No 2 (2024)
Publisher : Fakultas Kedokteran Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/JBiomedKes.2024.v7.262-267

Abstract

This paper draws attention to a recently identified sub-variant of SARS-CoV-2 known as EG 5, colloquially named "Eris," designated by the World Health Organization (WHO) as a variant of interest. The organization has urged nations to monitor and respond to the spread of this sub-variant since its identification on February 17, 2023. The discovery of the EG 5 variant, a sublineage of the Omicron variant, has introduced new challenges. Designated a "variant of interest" by the WHO, EG 5 carries distinctive genetic mutations, including an additional F456L amino acid mutation in the spike protein. Global prevalence has doubled, raising concerns about increased transmissibility. Although specific symptoms of EG 5 are not yet fully characterized, the variant has spread to 51 countries, with over 7,000 reported cases by August 15, 2023. The manuscript also explores the unique situation in Iraq, where, as of August 17, 2023, no EG 5 cases have been officially recorded. However, challenges such as self-treatment at. As the world grapples with the ongoing challenges of the COVID-19 pandemic, understanding the dynamics of emerging variants is crucial for effective public health responses and the development of targeted interventions.
Co-Authors Ahmed, Dina Alsayed, Raghda Bufaroosha, Muna Hairunisa, Nany Hameed, Seenar Husain, Amani Husnun Amalia, Husnun Hussein, Ahmed Kadhom, Mohammed Kudaer, Nuha B Mahdi, Sarah Mashabi, Yasmine Raheem, Rasha Rasheed, Malath Redwan, Amamer M Risan, Mohsen Hashim Sabrina, Isra Salman, Israa Thamer, Hamsa Yusop, Rahimi M Zaina, Nashita Amira Zainulabdeen, Khalid