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Growth of Bacteria Bacillus cereus in Liquid Media with Different Protein Sources Mukti, Pranata Rangga; Feliatra, Feli; Effendi, Irwan
Journal of Coastal and Ocean Sciences Vol. 1 No. 1 (2020): September
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

The aim of this research was to determine the optimal media composition and incubation time for the growth of Bacillus cereus and B. cereus isolate consortium with other strains isolate. Using an experimental method, which was testing 2 groups of bacterial isolates; B. cereus strain SN7 (isolate N) and bacterial consortium (combination of 5 isolates of B. cereus with different strains). Each group of isolates was grown in sago waste media added with two different protein sources (egg whites and skim milk) and 3 different concentrations in each protein source (8%, 10%, and 12%) with 3 replications in each treatment. Bacterial culture was measured every 6 hours for 24 hours using three methods; total agar plate count, spectrophotometric methods, and bacterial cell biomass methods. There was no significant difference between the growth of these two groups of bacterial isolates. Optimal growth of each isolate was in the sago waste media which was added with egg white but with different concentrations, namely 10% in B. cereus SN7 and 12% in the consortium. B. cereus consortium showed higher growth than B. cereus SN7. The optimal time for bacteria to grow was in the range of 12 to 24 hours
Growth of Bacteria Bacillus cereus in Liquid Mediums with Different Carbohydrate Sources Ningsih, Irma Ardila; Feliatra, Feli; Effendi, Irwan
Journal of Coastal and Ocean Sciences Vol. 1 No. 1 (2020): September
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Bacteria need a medium to grow Bacillus cereus is a probiotic bacterium. Molasses and tofu liquid waste are one of the wastes that are used as alternative medium, because they are easy to obtain, cheap, and contain nutrients that are high enough in the form of carbohydrates. This study aimed to determine the optimal medium for the growth of these bacteria. This study used a completely randomized factorial design. It consists of three factors, namely the type of waste (molasses + egg albumen and tofu liquid waste + egg albumen), then the combination of isolates (B. cereus SN7 and consortium), and the concentration of liquid waste used for medium growth is (molasses is 1%; 1.5% ; and 2% while tofu liquid waste is 8%; 10%; and 12%). Bacterial cell growth was measured by the Total Plate Count (TPC) method, spectrophotometric method, and biomass, while the incubation period for cell growth was measured every 0, 6, 12, 18, and 24 hours. The results of this study indicated that molasses + egg albumen with a concentration of 1% was the best medium for the growth of B. cereus bacteria along with consortium isolates
Biodegradability of Proteolytic Bacteria in Mangrove Ecosystems Claudia, Kristiwany Mayneke; Nursyirwani, Nursyirwani; Effendi, Irwan
Journal of Coastal and Ocean Sciences Vol. 2 No. 2 (2021): May
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

The purpose of this study was to awareness the optimal time for the growth of proteolytic bacteria in producing the protease enzyme and determine the effectiveness of the protease enzyme from proteolytic bacteria in degrading mangrove litter. The method used was the experimental method by using two proteolytic bacteria isolates, namely Bacillus manliponensis (K6) and Bacillus toyonensis (K20). This method used one treatment for each proteolytic bacterial isolate, namely the addition of different extract containing protease enzyme (0%, 25%, and 50%) with three replications. Measurement of bacterial growth was carried out every 6 hours for 24 hours using Total Plate Count (TPC) and spectrophotometric method. B. manliponensis and B. toyonensis have enzyme activity as indicate by the presence of a clear zone on Zobell Marine Agar 2216 and skim milk 1%. The growth bacteria based on the TPC measurement was directly proportional to the spectrophotometric measurement results. The highest bacterial count was (1.39 x 108 CFU's/ml) K6 bacteria and (1.52 x 108 CFU's/ml) K20 bacteria. The results of the measurement of cell growth in the spectrophotometric method occurred at the 6th hour, namely 10.36 x 108 CFU's/ml (K6 bacteria) and 10.97 x 108 CFU's/ml (K20 bacteria). The optimum time of the protease enzyme occurred at 6 hours, which was 0.0258 mg/ml (K6 bacteria) and 0.0262 mg/ml (K20 bacteria). The highest dissolved protein content was obtained at the 50% dose of 0.054 mg/ml (K6 bacteria) and 0.055 mg/ml (K20 bacteria), while the lowest was at 0%, namely 0.050 mg/ml (K6 bacteria) and 0.051 mg/ml (K20 bacteria).
Water Pollution Levels Based on Organic Matter Parameters and Amount of Escherichia coli Bacteria in the Mosque River Estuary of Dumai City Abmi, Cyndy; Nedi, Syahril; Effendi, Irwan
Journal of Coastal and Ocean Sciences Vol. 2 No. 2 (2021): May
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

This research was conducted in March 2020 at Mosque River Estuary of Dumai City, Riau Province. The aimed of this research was to determine the pollution levels in the Mosque River Estuary based on organic matter parameters and E. coli bacteria. DO analysis refers to SNI 06.6989.14-2004, BOD5 refers to SNI 06-2503-2009, BOT refers to SNI 06-6989.22-2004 and E. coli bacteria refers to SNI 2897: 2008. The results of DO measurements ranged from 5.30 - 5.56 mg/l, BOD5 ranged from 16.5-21.8 mg/l, BOT ranged from 15 - 162 mg/l, and bacteria E. coli ranges from 11.1 - 1,100 MPN/100ml. Based on the results of the account of the Pollution Index, the Mosque River Estuary waters of Dumai City, Riau Province, are categorized as light to moderate pollution.
Level of Water Pollution Based on Organic Material Parameters and Number of Bacteria Escherechia coli in Dumai River Estuary, Dumai City Lubis, Nur Aisyah; Nedi, Syahril; Effendi, Irwan
Journal of Coastal and Ocean Sciences Vol. 2 No. 2 (2021): May
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

This research was conducted in March 2020 at the Dumai River Estuary, Dumai City, Riau Province. The purpose of this study was to analyze the parameters of OT, BOD and TOM in the Dumai River Estuary; analyzing E. coli bacteria in the Dumai River Estuary and Determining the Level of Water Pollution Based on the Content of Organic Material Parameters and E. coli in the Dumai River Estuary. The data analysis method refers to OT (SNI 06.6989.14-2004), BOD (SNI 06-2503-2009), BOT (SNI 06-6989.22-2004) and E. coli (SNI 2897-2008). The results of measuring the parameters of organic matter content in the Dumai River Estuary, OT levels ranged from 4.1 - 4.6 mg /L, BOD5 levels ranged from 21.5 - 30.6 mg /L, the TOM content in The Dumai River Estuary ranges from 104 -165 mg /L and E. coli bacteria in the Dumai River Estuary ranges from 180 - 764 MPN /100 mL. The level of pollution at the Dumai River Estuary is based on the parameters of organic matter and E. coli content, including the criteria for light pollution
Prevalence Analysis of Hypodermal Infectious and Haematopoetic Necrosis Virus (IHHNV) in Vaname Shrimp (Litopenaeus vannamei) in Bengkalis District Fahmi, Adzra Ariesta; Feliatra, Feli; Eff, Irwan; Muhson, Nanang
Journal of Coastal and Ocean Sciences Vol. 3 No. 3 (2022): September
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

The disease is a limiting factor and causes economic losses in the cultivation of white shrimp (Litopenaeus vannamei). Diseases in vaname shrimp are caused by viruses, microbes, parasites, organisms, and natural conditions. One of these diseases is infectious hypodermal and hematopoietic necrosis (IHHNV). This study was conducted from February to April 2022. This study aimed to analyze the prevalence of IHHNV that attacks white shrimp in the Bengkalis Regency and to observe and observe clinical symptoms in whitewashed shrimp infected with IHHNV in the pond area of the Bengkalis Regency. The research method used in this study is a survey method. The primary data taken included water quality observations, morphological observations, sampling, and detection of IHHNV using the polymerase chain reaction (PCR) method. The results showed that the prevalence of IHHNV in white shrimp was 0%. PCR testing using a specific primer measuring 392bp showed that white shrimp samples from three locations in Bengkalis District, namely Bengkalis District, Bukit Batu District, and Rupat District, were negative for IHHNV and did not show any clinical symptoms of IHHNV on morphological examination
Prevalence Analysis of WSSV (White Spot Syndrome Virus) in Vaname Shrimp (Litopenaeus vannamei) in Bengkalis District Akbar, Sidiq Malik; Effendi, Irwan; Feliatra, Feli; Muhson, Nanang
Journal of Coastal and Ocean Sciences Vol. 3 No. 3 (2022): September
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Vannamei shrimp (Litopenaeus vannamei) is one type of shrimp that is widely cultivated in Indonesia. Vannamei shrimp dominates aquaculture in Indonesia which was previously dominated by tiger shrimp (Penaeus monodon). WSSV, a double-stranded circular DNA virus, has become one of the most widespread and fatal diseases in shrimp populations. Therefore, it is necessary to conduct research to detect the WSSV virus that attacks white vaname shrimp and determine the prevalence rate of the WSSV virus that attacks white vaname shrimp in Bengkalis Regency. This study was conducted from February to June 2022. The purpose of this study was to analyze the prevalence rate of the WSSV virus that attacks white shrimp in the Bengkalis Regency. The research method used in this study is a survey method. The results of the morphological examination did not show any clinical symptoms in vaname shrimp. Tests using PCR with specific primers measuring 941 bp showed DNA bands of vaname shrimp samples from 3 locations, namely Bengkalis district, Bukit Batu district and Rupat district. So the prevalence of the research results obtained is 0%.
Analysis of the Effectiveness of Mango Leaves (Mangifera indica) as a Phytoimunostimulant on Common Carp (Cyprinus carpio) in Brackish Water Saputra, Andika; Effendi, Irwan; Mardalisa, Mardalisa
Journal of Coastal and Ocean Sciences Vol. 4 No. 1 (2023): January
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

The problems that are often experienced when cultivating goldfish are diseases caused by pathogens that attack cultivated fish. The cause of disease in fish is caused by interactions between fish, pathogens and their habitat environment that can cause death in fish. The solution to this problem is to provide a phytoimmunostimulant derived from mango leaves (Mangifera indica). The purpose of this study was to determine the effect of mango leaves on the hematology and physiology of common carp. This research was conducted in January-April 2022. The method used in this research is an experimental method by comparing data. Parameters observed were total erythrocytes, hemoglobin, hematocrit, blood glucose, absolute body weight growth, survival rate, and water quality. Based on the research that has been done, it can be concluded that the addition of 1.5% mango leaf powder into fish feed as a phytoimmunostimulant affects the hematological and physiological effects of common carp. Common carp treated with phytoimmunostimulants in fresh water had higher mean values ​​(total erythrocytes 1.75x106 cells/mm3, hematocrit 33.66%, and hemoglobin 8.13 g/dL) compared to data in brackish water (total erythrocytes 1.54x106 cells/mm3, hematocrit 30.66%, and hemoglobin 7.76 g/dL).
Analysis of the Relationship Between Phytoplankton Abundance and Total Oil Content in Bayur Bay Waters, Padang City, West Sumatera Province Ghifari, Muhammad Fadil; Nurrachmi, Irvina; Effendi, Irwan
Journal of Coastal and Ocean Sciences Vol. 5 No. 3 (2024): September
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jocos.5.3.166-174

Abstract

The research was conducted in January - February 2024 in the waters of Teluk Bayur, Padang City, West Sumatra Province. This study analyzes the relationship between total oil content and phytoplankton abundance in Bayur Bay waters. The method used in this study is a survey method with a purposive sampling technique. Based on the results found. The value of oil content at each station and sampling point varies. The highest value is obtained at station III with an average value of 0.00005387 ppm, while the lowest is obtained at station IV with a content value of 0.00001153 ppm. In the Bayur Bay waters, 16 species of phytoplankton were found, consisting of Bacillariophyceae (10 species), Cyanophyceae (2 species), Coscinodiscophyceae (2 species), Dinophyceae (1 species), Raphidophyceae (1 species). Phytoplankton abundance in Bayur Bay waters shows different values for each station, where the highest average phytoplankton abundance is found at station IV, with a total of 9216.55 (ind/L). In contrast, the lowest abundance is found at station I, with a total of 1016.77 (ind/L). The results of linear regression analysis, the relationship between phytoplankton abundance and oil content in Bayur Bay waters at each station obtained a mathematical equation y = 6245.3 + (-7E+07x) with a coefficient of determination R2 = 0.3016 and the correlation coefficient r = 0.5491. With the conclusion that the relationship between phytoplankton abundance and total oil content in Bayur Bay waters is included in the average group.
EFFECTIVENESS TEST OF NIPAH EXTRACT AS LARVACIDE ON MOSQUITO LARVAE (Aedes aegpti) Purba, Thadeus Mariode Triputra; Effendi, Irwan; Feliatra, Feli
Asian Journal of Aquatic Sciences Vol. 2 No. 1 (2019): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (607.705 KB)

Abstract

This research was conducted in January - February 2019. The purpose of this study was to examine the effectiveness of mangrove extracts on stems and fruit on the growth of Aedes aegypti mosquito larvae and to know Lethal Concentration LC50 and Lethal Time LT50. The method used is the experimental method, with the experimental design is a completely randomized design (CRD). The treatment of nipah fruit and stem extract concentrations was 3%, 6% and 9% with contact time of 15 minutes, 30 minutes, 45 minutes 60 minutes, 120 minutes, 480 minutes and the number of replications 3 times with 2 trials. To find out (LC50) and (LT50), the data was analyzed using the SPSS program. The sample of this research is stem and nipah fruit taken in the mangrove area of Kayu Ara Village, Siak Regency. Aedes aegypti larvae are taken at a water reservoir next to the Arfaunas Mosque in Tampan District. The results showed that the stem and nipah fruit extracts had effective killing power against Aedes aegpti larvae. The extract that was more effective in killing mosquito larvae was the nipah fruit extract compared to the nipah stem extract seen in the number of larval deaths with LC50 = 3.398% and LT50 = 3.540 hours. This shows that the level of toxicity is quite toxic.
Co-Authors abmi, cyndy Adriman, Adriman Afrizal Tanjung Afrizani, Syafrinaldi Ahmed Al-Harbi Akbar, Sidiq Malik Al Khudri Sembiring Andesca, Yofi Devara Andika Rismayanti Hadi ANDIKA SAPUTRA Anwar, Sagita Aqil Fikar Rivai Aras Mulyadi Arrafiqur Rahman Asmika Harnalin Simarmata Asri Mursawal Batubara, Ummi Mardhiah Bintal Amin Cahyani Fitrah Tanjung Christine M Situmeang Claudia, Kristiwany Mayneke Daulay, Anita Diah Permata Sari Dessy Yoswaty Dessy Yoswaty Dessy Yoswaty Desy Mutia Sari Dewan Pratama Novian Dimas Gusriansyah Dimas Gusriansyah Eddiwan Kamaruddin Efawani Efawani Efriyeldi, Efriyeldi Elizal Elizal Elizal, Elizal Emilda Firdaus Eriani, Eti Evi Deliana HZ Eza Buana Fatwa Eza Buana Fatwa Fahmi, Adzra Ariesta Fakhrurozi Fakhrurozi Farma, Elmi Aulia Fathoni, Ahmad Yusron Feliatra Feliatra, Feli Fiki Harjuni firda laily mufid Ghifari, Muhammad Fadil Gusriansyah, Dimas Halima, Asyatul Hanafi Hussin Hanies Ambarsari Hanryani, Putri Henni Syawal Henny Syawal Henny Syawal HERI SYAHFITRI Ida Ayu Eka Putri Yuliandari Ilham Ilahi Indra Lesmana Indra Suharman Irvina Nurachmi Irvina Nurrachmi Ita Widowati Joko Samiaji Judita Grace Merry Nainggolan Jusup Suprijanto Lamun Bathara Lubis, Nur Aisyah Lufita Nur Alfiah M. Yusuf Mardalisa, Mardalisa Marta Dinata MARTONO MARTONO Masdini, Alvira Masjudi, Heri May Juna Tanjung Mega Novia Putri Mega Novia Putri Mega Novia Putri Mira Angelina Syah Mochammad Sukedi Moritania, Rizky Muhammad Aliv Kusuma Putra Muhammad Aliv Kusuma Putra Muhammad Arief Wibowo Muhammad Hafizh Muhammad Natsir Kholis Muhammad Razali Muhammad Rizal Razman Muhson, Nanang Mukti, Pranata Rangga Musyirna Rahmah NST Mutia Dhiya Amani Mutia Dhiya Amani Mutiara Naimi Nasution Nasution, Nisfi Maulidia Nicholas Renaldo Ningsih, Irma Ardila Nisfi Maulidia Nasution Nur Ikhlas Syuhada Nuraini Nuraini Nursyafni Nanggola Nursyirwani Nursyirwani Nursyirwani Nursyirwani Nursyirwani Nursyirwani Nursyirwani, Nursyirwani Nyoto Okta Rizal Karsih Okta Rizal Karsih Parbo, Annisa Presti Povy Hutabarat Pulungan, Amar Doli Purba, Thadeus Mariode Triputra Purwantoro - Putra, Muhammad Hendra Putri Wangi Tri Rumelia Putri, Mega Novia Rady, Rady Rahmat Ramadansur Rianti Putri Rika Permata Sari Rika Permata Sari Riswan, M Rita Wijaya Rita Wiyati Riza, Subkhan Rodhi Firmansyah Roiman Pakpahan Ronal Kurniawan Ronal Kurniawan Ronal Kurniawan Ruthmawati, Abigael Serly Silviyanti Sibarani, Michael Parluhutan Jupiter Silitonga, Lamtiur Rotua Siti Ardiah Soni Sanjaya Soni Sanjaya Sony Sanjaya Sri Wahyuni Sri Wahyuni Sri Wahyuni Suryadi Saputra Suryadi Saputra Syafruddin Nasution Syahputra, Tomi Syahril Nedi Syaputry Lita Yanti T. Budiardi Tampubolon, Kristanti Tanjung, Cahyani Fitrah Tengku Rany Oktaviany Tengku Rany Oktaviany Thamrin Thamrin Three Yulinda Tio Afandi Nasution Tomi Syahputra Tri Vani Bernadetha Ginting Trisla Warningsih Tubagus Hasanuddin Ulfi Laili Astika Ulfi Laili Astika Ummi Mardhiah Batubara Usman M Tang Usman Muhammad Tang Valentina Dian W. Cahyaningrum Windarti Windarti Windarti Windarti Windarti Windarti Yetti Elfina Yudia Anggun Kirana Zulkarnaini Zulkarnaini Zulkifli Zulkifli ZULKIFLI ZULKIFLI Zulkifli Zulkifli