Ratna Sholatia Harahap
Department Of Animal Husbandry, Faculty Of Animal Science, Universitas Jambi.

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Gambaran Darah dan Status Kesehatan pada Kambing Peranakan Etawa (PE) yang Mengalami Abortus: Laporan Kasus Wibowo, Sarwo Edy; Harahap, Ratna Sholatia; Nugraheni, Yudhi Ratna; Awaludin, Aan; Rahayu, Pudji; Rosadi, Bayu; Yatno, Yatno; Syarifuddin, Ahmad; Damhuri, Dedi; Safitri, Jessica Anggun
Jurnal Ilmiah Ilmu-Ilmu Peternakan Vol 28 No 1 (2025): Mei 2025
Publisher : Fakultas Peternakan Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/jiiip.v28i1.36323

Abstract

Latar Belakang: Abortus merupakan masalah reproduksi yang dipengaruhi oleh status kesehatan ternak yang menyebabkan kerugian pada ternak seperti kambing. Tujuan: Penelitian ini dilakukan untuk mengetahui gambaran darah dan status kesehatan pada kambing yang mengalami abortus di Fapet Farm Fakultas Peternakan Universitas Jambi. Metode: Metode yang digunakan dalam penelitian ini diawali dengan pencatatan identitas ternak, anamnesa, dan pemeriksaan fisik pada kambing PE yang menunjukkan gejala klinis sakit. Sampel darah dan feses kemudian diambil dari lima ekor kambing yang mengalami abortus untuk dilakukan pemeriksaan telur cacing, parasit darah, dan serologi terhadap Brucella sp yang diduga sebagai penyebab terjadinya abortus. Hasil: Hasil analisis pemeriksaan darah dan kesehatan yang dilakukan ditemukan bahwa adanya infeksi parasit pada pada lima kambing yang mengalami abortus. Empat kambing yang terinfeksi parasit Anaplasma sp., yaitu kambing 2, kambing 3, kambing 4, dan kambing 5. Dua kambing positif terinfeksi parasit gastrointestinal, yaitu ditemukan telur cacing Paramphistomum sp. dan Haemonchus sp., pada kambing 1, sedangkan kambing 2 ditemukan telur cacing Paramphistomum sp. dan Moniezia sp. Hasil pemeriksaan hematologi terdapat peningkatan leukosit pada kambing nomor 5 dan penurunan nilai hematokrit pada kelima kambing dan MCHC pada kambing nomor 3 dan 4. Kesimpulan: Kondisi abortus yang dialami kambing PE di Fapet Farm Fakultas Peternakan Universitas Jambi terjadi karena adanya infeksi parasit, peningkatan leukosit, dan penurunan nilai hematokrit yang ditemukan pada sampel darah dan feses yang diuji.
Association of Gene Growth Hormone (GH) Diversity with Quantitative Characteristics of Crossing Merawang Chickens with Arabic Chickens Muhammad, Mus'ab; Depison; Gushairiyanto; Harahap, Ratna Sholatia
Jurnal Ilmu-Ilmu Peternakan Vol. 35 No. 1 (2025): April 2025
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2025.035.01.2

Abstract

The growth hormone (GH) gene plays a crucial role in regulating the growth and metabolism of Chickens. This study investigates the polymorphism of the GH gene and its association with growth traits in chickens resulting from the crossbreeding of Merawang and Arabs (MeA). The research began by raising 60 chickens—30 males and 30 females—from the Merawang and Arabic chicken cross for four months. Various growth traits, including weight, weight gain, and body size, were measured. Blood samples from the MeA chickens were collected for DNA analysis to assess GH gene polymorphism. The analysis involved DNA extraction, PCR amplification, and RFLP using the Taql enzyme. The data were analysis using T-test to evaluate the association of the GH gene polymorphism with growth traits. The results showed that the male of crosses between Merawang and Arab chicken (MeA) have higher significant (P<0.05) growth traits, including body weight, weight gain, and body size than female MeA chickens. The chest circumference is a defining characteristic of body size in male and female MeA chickens, while the upper body length is a crucial feature of body shape. The GH gene were found polimorpic with three genotypes, including GG, AG, and AA. The GH gene polymorphisms have a significant association (P<0.05) with growth traits, including body weight, body weight gain, and body size. Chicken with GG genotype have a higher growth trait than others. The GH gene can be used as a potential genetic marker for selection for improving chicken growth.
Association of Growth Hormone Genes with Performance of Crossbreeds of Sentul Chicken and Arab Chicken Using PCR-RFLP Sinaga, Josua; Depison, Depison; Gushairiyanto, Gushairiyanto; Harahap, Ratna Sholatia
Jurnal Agripet Vol 25, No 2 (2025): Volume 25, No. 2 October 2025
Publisher : Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v25i2.42764

Abstract

This study evaluated the body weight, weight gain, body measurements, and genetic diversity of the Growth Hormone (GH) gene in male and female Sentul-Arab (SeA) crossbred chickens. It also explored the association between the GH gene and these traits. The research involved 30 male and 30 female SeA chickens reared from day-old chicks (DOC) to four months of age. The experimental method used direct observation to collect data, including body weight, weight gain, body measurements, and blood samples. Data were analyzed using t-tests, the Hotellings T test, and Principal Component Analysis (PCA). Molecular analyses assessed genotype and allele frequencies, Hardy-Weinberg equilibrium, heterozygosity levels, and Polymorphic Information Content (PIC). The results indicated that male SeA chickens had significantly higher (P0.05) body weight, weight gain, and body measurements compared to females. GH gene analysis revealed polymorphism, with genotype frequencies of +/+ (0.43), +/- (0.35), and -/- (0.22), consistent with Hardy-Weinberg equilibrium. The heterozygosity levels indicated moderate genetic diversity, while the PIC value fell within the low category. In conclusion, male SeA chickens exhibited superior body weight, weight gain, and body measurements compared to females, with chest circumference and shank length being critical indicators of body size. The polymorphic GH|TaqI gene was associated with body weight, weight gain, and body measurements, with the +/+ genotype showing the most favorable effects.