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Dirja, Bayu Tirta
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Journal : International Journal of Cell and Biomedical Science

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X-Ray Scanning Reduce Soluble Active Molecules of Mesenchymal Stem Cells Putra, Agung; Pasongka, Zenitalia; Widyatmoko, Agus; Prasetio, Ardi; Dirja, Bayu Tirta
International Journal of Cell and Biomedical Science Vol 1 No 1 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i1.14

Abstract

Mesenchymal Stem Cells secrete various anti-inflammatory and regenerative SAMs-MSCs that possess immunomodulatory properties and may accelerate wound healing. As a potential agent for therapeutic, SAMs-MSCs should be stable in any condition, including under X-Ray Scanning. The previous study reveal that X-Ray Scanning may induce protein damage. However, the investigation regarding the stability of SAMs-MSCs under X-Ray Scanning is limited, thus this study aimed to investigate the stability of SAMs-MSCs after X-Ray Scanning. Mesenchymal Stem Cell medium was filtrated using the TFF method at 300 and 5 kDa filter cut off and sterilized using 0,1 um syringe. The SAMs-MSCs underwent X-ray Scanning using public Air Port X-Ray twice. The SAMs-MSCs concentration was measured using ELISA. T-test analysis was performed for the statistical analysis with P<0,05. This study revealed that X-ray scanning reduce the concentration of SAMs-MSC. A previous study found that x-ray irradiation may damage protein at 6–18 keV caused by the energy deposited by photoelectrons that are generated by the interaction of X-ray photons and the protein leading to photoelectron-induced damage.
Co-Authors Agung Putra Agus Widyatmoko, Agus Ajmala, Indana Eva Andari, Marie Yuni Candra Satria Irawan, Risky Citami, Niko Didit Yudhanto Eustachius Hagni Wardoyo GD, Ni Made Saithanya Ida Ayu Eka Widiastuti Ilham, M. Arya Rifqi Ilsa Hunaifi, Ilsa Kadriyan, Hamsu Nugraha, Kadek Nandita Nuriastuti, Novera NURUL HIDAYAH P, Novanda Dila Pasongka, Zenitalia Prasetio, Ardi Putri, Dyah Ayu Rafiq, Azhar Ramdhani Kusuma, Dinie Rohadi Rohadi Sa’dyah, Nur Anna C Saputra, Nurman Sari, Putu Suwita Wahyu Sulistya Affarah, Wahyu Sulistya Yasmine Azzahara, Salma Yoga Pamungkas Susani