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All Journal Biology, Medicine, & Natural Product Chemistry Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Abdimas Galuh : Jurnal Pengabdian Kepada Masyarakat Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Juwita, Syntia Tanu
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Health Professions Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Neuroscience Nursing Public Health

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Comparison of Proliferation and Apoptosis in CD34+ Lymphoblasts in Pediatric Acute Lymphoblastic Leukemia: invivo and exvivo Conditions Hernaningsih, Yetti; Cahyadi, Andi; Rusanti, Rahmi; Armayani, Erawati; Juwita, Syntia Tanu; Nur‘ Aini, Farida; Tanzilia, May Fanny; Nunki, Nastasya
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 32 No. 1 (2025)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v32i1.2389

Abstract

The proliferation and apoptosis assays have been utilized in numerous studies to develop new substances such as antineoplastic agents. Commonly, it was performed in ex-vivo conditions using the culture method. However, the cytotoxic or cytostatic effects observed ex vivo often differ from those in vivo. This study investigated differences in proliferation and apoptosis of lymphoblast between in vivo and ex vivo conditions of childhood ALL. This study was conducted on new childhood acute lymphoblastic leukemia (ALL) patients. Nineteen (19) subjects were recruited, comprising of 10 with favorable and 9 with unfavorable outcomes. The negative control came from 8 healthy children volunteers. All patients under went leukemia immunophenotyping, including CD34, to identify the phenotype. Bone marrow mononuclear cells (BMMCs) of patient groups were analyzed using apoptosis and proliferation assays, as well as the negative control group, and then compared to the in vivo condition. 12 out of 19 BMMC were cultured for 48 hours, and proliferation and apoptosis assays were performed in ex vivo conditions. The results showed that the proliferation, apoptosis, and apoptosis/proliferation (A/P) ratio of the patients in the ex vivo group were significantly higher than the in vivo group with p =0.000, p =0.050, and p =0.000, respectively. The proliferation was higher for patient groups than the control group, with p =0.001 and p =0.004, respectively. The apoptosis rates of the patient group were higher than the control group, with p =0.000 and p =0.002, respectively. The proliferation and apoptosis of lymphoblasts ex vivo are higher than in vivo.
Co-Authors Alves, Silvina Sandra X. Amanda, Cornelia Andi Cahyadi Antoro, Ester Lianawati Armayani, Erawati Datin An Nisa Sukmawati Edda, Ilda Rambu Elfred Rinaldo Kasimo, Elfred Rinaldo Fendy Prasetyawan Freitas, João Gregório Freitas, Maria Do Carmo Da Costa Hilmi, Mochammad Hanif Laili, Nur Fahma Mahardika, Juan Vega Mebung, Konradus Klala Neni Probosiwi Nunki, Nastasya Nur‘ Aini, Farida Ornai, Andre Mario Sequeira Alves Rahmi Rusanti, Rahmi Retnowati, Ana Rochmad Krissanjaya savitri, Lisa Tanzilia, May Fanny Tsamrotul Ilmi Wulansari, Ida Septika Yanti, Novirma Yetti Hernaningsih Yuneka Saristiana