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All Journal Biology, Medicine, & Natural Product Chemistry Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Abdimas Galuh : Jurnal Pengabdian Kepada Masyarakat Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Juwita, Syntia Tanu
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Health Professions Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Neuroscience Nursing Public Health

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In Silico BCell Epitope Design of Zika Virus Vaccine Using “Zika Virus Isolate Zika Virus/H sapiensTc/Tha/2006/CVD_06020 Complete Genome” Savitri, Lisa; Juwita, Syntia Tanu; Antoro, Ester Lianawati; Wulansari, Ida Septika; Sukmawati, Datin An Nisa; Freitas, João Gregório; Ornai, Andre Mario Sequeira Alves
Biology, Medicine, & Natural Product Chemistry Vol 12, No 2 (2023)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2023.122.525-531

Abstract

ZIKV has been found in the cerebrospinal fluid (CSF) and brains of adults infected with viruses that manifest neurological disorders. ZIKV is a mosquito-borne single-stranded RNA virus, which belongs to the family Flaviviridae. The efforts of the scientific community have rapidly increased knowledge about this virus. However, understanding the complexities of ZIKV infection, transmission and pathogenesis remains an urgent challenge. Therefore, it is critical to study competent vectors and natural reservoirs for ZIKV, viral genetic diversity and flavivirus coinfection. Due to the great challenges to develop a ZIKV vaccine, it is still not possible to be immunized against ZIKV infection and related pathologies. The methods are nucleotide search for the Zika virus was carried out in silico, using the NCBI bioinformatics application by providing access to biomedical and genomic information. /H. sapiens-tc/THA/2006/CVD_06-020, the complete genome was then searched for FASTA, then prediction of vaccine epitope using the IEDB. The vaccine candidate peptides were analyzed for their antigenicity using VaxiJen. Proteins were classified by AllerTop to known allergenicity, then ToxinPred to predict and design toxic/non-toxic peptides. There are 30 peptide sequences are predicted to be a candidate of peptides B-cell epitope zika virus vaccine design using “zika virus isolate zika virus/H. sapiens-tc/THA/2006/CVD_06-020, complete genome”.
Leaf Extract of Kentut (Paederia foetida L.) as a Preventive Measure Against Interleukin-6 Expression in the Liver of Mice in a Sepsis Model Injected with Escherichia coli Savitri, Lisa; Freitas, Maria Do Carmo Da Costa; Kasimo, Elfred Rinaldo; Krissanjaya, Rochmad; Juwita, Syntia Tanu
Biology, Medicine, & Natural Product Chemistry Vol 13, No 2 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.132.329-336

Abstract

Kentut leaves (Paederia foetida L.) are a medicinal plant that can be used as a preventative medicine against sepsis. This plant contains secondary metabolite compounds such as alkaloids, flavonoids, triterpenoids, saponins, and other active compounds. This research aims to to determine the influence and effective dosage of Kentut leaf extract as a preventive measure against IL-6 expression in the livers of mice in a sepsis model injected with E. coli. The method used was a Completely Randomized Design (CRD). The study involved 24 white male mice divided into 6 groups. Data analysis was performed using One way ANOVA. The average values of IL-6 expression in the mouse livers for each group are as follows: KN at 7.09%±0.06; K+ at 26.36%±0.02; K- at 72.60%±0.05; PI (100mg/kgBW) at 71.04%±0.04; PII (300mg/kgBW) at 62.22%±0.02; and PIII (500mg/kgBW) at 40.92%±0.01. The research results indicate an influence of kentut leaf extract as a preventive measure against IL-6 expression in the livers of mice in the sepsis model injected with E. coli, with a significance value of 0.000 or p-value < 0.005. The effective dosage of kentut leaf extract as a preventive measure against IL-6 expression is the PIII dosage of 500mg/kg BW. The anti-inflammatory mechanism in sepsis is thought to be caused by the presence of flavonoids, alkaloids, phenolic acids, and terpenoid compounds. The most likely anti-inflammatory mechanism is believed to involve flavonoids inhibiting cyclooxygenase (COX) and lipoxygenase (LOX) enzymes involved in the synthesis of inflammatory mediators such as prostaglandins and leukotrienes, which can trigger IL-6 production.
Effect of Female Age on Crossing Over Frequency in Drosophila melanogaster Crosses N x bcl and N x ym and Their Reciprocals Savitri, Lisa; Kasimo, Elfred Rinaldo; Krissanjaya, Rochmad; Juwita, Syntia Tanu; Antoro, Ester Lianawati; Wulansari, Ida Septika
Biology, Medicine, & Natural Product Chemistry Vol 12, No 1 (2023)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2023.121.267-271

Abstract

Crossing over is the occurrence of disconnection and reconnection followed by a reciprocal exchange between the two chromatids in a bivalent form. The crossing event will produce parental type and recombinant type. In the event of crossing over, various factors can influence it. These factors can be due to internal and external. Recently, various factors have been reported that influence the incidence of crossing over. These factors include age, temperature, radiation, and changes in chromosome structure. This research is a type of experimental research that uses a randomized block design. Randomized block design by crossing D. melanogaster strains ?N>< ?bcl and ?N>< ?ym and their reciprocals. From the results of this cross (F1) then cross again ?N with the recessive male (from stock) then observe the phenotype of the offspring (F2) and calculate the results of the offspring. The F2 ?N crosses were treated with age variations, namely 0, 3, 6, 9, 12, 15, and 18 days. Based on the results of these crosses, the derived strains that appeared in the F2 crosses showed the phenomenon of crossing over with the influence of the age of the female and the type of strain on crossing events. the frequency or value of crossing over (formation of recombinants) decreased with the increasing age of the female. If the age of the female affects the frequency of crossing over, then the older the female, the more likely the frequency of crossing over will decrease. However, because the data obtained were incomplete, it was not possible to know the effect of female age on the frequency of crossing over of D. melanogaster crosses ?N>< ? bcl and ?N >< ?ym and their reciprocals. The condition for crossing over is the formation of a synaptonemal complex. Age of D. melanogaster females has an effect on the frequency of crossing over in crosses N? >< bcl?, N? >< ym?, and their reciprocals. The older Drosophila melanogaster is, the lower the frequency of crossing over will occur. Based on this, it was necessary to cross D. melanogaster with strains N, bcl, and ym. A cross consists of ?N ><?bcl and ?N ><?ym and their reciprocals. By crossing ?N with a recessive male from the stock, then observing the F2 phenotype, it is hoped that crossing over will occur. So that you can better understand by doing the practice directly. In this case, the effect of crossing over is seen from the age of the female and the type of strain.
Histopathological Description of Mouse Liver in a Sepsis Model Infected with Escherichia coli Treated with Paederia foetida L. Leaf Extract for Sepsis Prevention Savitri, Lisa; Retnowati, Ana; Kasimo, Elfred Rinaldo; Krissanjaya, Rochmad; Juwita, Syntia Tanu
Biology, Medicine, & Natural Product Chemistry Vol 13, No 2 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.132.485-492

Abstract

The leaf of Paederia foetida L. is one type of medicinal plant that can be used as a preventive medicine for sepsis. This plant contains secondary metabolites such as alkaloids, flavonoids, triterpenoids, saponins, and other active compounds. The objective of this study was to determine the histopathological description of the liver in a mouse sepsis model infected with E. coli, with the administration of Paederia foetida L. leaf extract for sepsis prevention, and to ascertain the influence and effective dosage of the leaf extract as a preventive measure against liver histopathology in the sepsis model induced by E. coli. The method employed was a Completely Randomized Design (CRD). The study used 24 male white mice divided into 6 (six) groups. Data analysis was conducted using One Way ANOVA. The results of the study revealed the histopathological profile of liver cell degeneration in group PI (100mg/kg BW) at 20.79%±0.03, group PII (200mg/kg BW) at 21.63%±0.02, and group PIII (500mg/kg BW) at 9.08%±0.02. Necrosis rates were observed in group PI (100mg/kgBW) at 22.62%±0.04, group PII (200mg/kg BW) at 17.63%±0.02, and group PIII (500mg/kg BW) at 6.05%±0.02. The presence of polymorphonuclear leukocytes (PMN) was detected in group PI (100mg/kgBW) at 39.56%±0.03, group PII (200mg/kgBW) at 28.05%±0.02, and group PIII (500mg/kg BW) at 18.45%±0.03. The test results showed a significant effect of P. foetida L. leaf extract as a preventive measure against liver histopathology in the mouse sepsis model infected with E. coli, with significant values for necrosis (p=0.000), cell degeneration (p=0.000), and PMN (p=0.000). The most effective dosage of P. foetida L. leaf extract as a preventive measure against liver histopathology in the mouse sepsis model infected with E. coli was the dosage used in group PIII (500mg/kgBW).
PREVENTION OF GASTROPROTECTIVE ACTIVITY OF CHINESE BETEL EXTRACT (PEPEROMIA PELLUCIDA L.) ON THE HISTOPATHOLOGICAL FEATURES OF THE STOMACH IN A MOUSE MODEL OF GASTROENTERITIS Savitri, Lisa; Prasetyawan, Fendy; Saristiana, Yuneka; Juwita, Syntia Tanu; Amanda, Cornelia; Mebung, Konradus Klala
Jurnal Bioteknologi dan Biosains Indonesia Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jbbi.11.2.8156

Abstract

Gastroenteritis is a common gastrointestinal disorder characterized by inflammation of the stomach and small intestine, often leading to symptoms such as diarrhea, vomit-ing, and abdominal pain. This study investigates the gastroprotective effects of Pepe-romia pellucida (Chinese betel) extract on histopathological features of the stomach in a mouse model of gastroenteritis. Male Swiss strain mice, aged 2-3 months, were treated with different doses of P. pellucida extract (100, 300, and 500 mg/kg BW) for 7 days after inducing gastroenteritis with Escherichia coli infection. Histopathological ex-amination showed that the extract significantly reduced cell degeneration, necrosis, and polymorphonuclear leukocyte infiltration in the stomach compared to the negative control group. The highest dose (500 mg/kg BW) exhibited the most prominent gastro-protective effect. These results suggest that P. pellucida possesses promising thera-peutic potential for gastroenteritis, possibly due to its bioactive compounds such as flavonoids, tannins, and saponins. Further studies are needed to explore the underly-ing mechanisms and optimize its therapeutic application.
THE ANTI-APOPTOTIC POTENTIAL OF Paederia foetida L. LEAF EXTRACT THROUGH THE DOWNREGULATION OF CASPASE-3 EXPRESSION IN AN ESCHERICHIA COLI-INDUCED SEPSIS MICE MODEL Savitri, Lisa; Krissanjaya, Rochmad; Juwita, Syntia Tanu; Sukmawati, Datin An Nisa; Probosiwi, Neni; Laili, Nur Fahma; Ilmi, Tsamrotul; Prasetyawan, Fendy
Jurnal Bioteknologi dan Biosains Indonesia Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jbbi.11.2.8235

Abstract

Sepsis is a life-threatening condition characterized by an abnormal immune response to infection, leading to high mortality rates in intensive care units (ICUs) worldwide. Caspase-3, a crucial enzyme in the apoptosis pathway, plays a significant role in sep-sis-related cellular damage. This study investigates the anti-apoptotic potential of Paederia foetida L. leaf extract by examining its effect on Caspase-3 expression in an Escherichia coli-induced sepsis mice model. Male Balb/c mice were divided into six groups, including positive control, negative control, and treatment groups receiving varying doses of the extract (100 mg/kgBW, 300 mg/kgBW, and 500 mg/kgBW). Caspase-3 expression in the spleen was measured after 24 hours of treatment. The results demonstrated a significant reduction in Caspase-3 expression, particularly in the group treated with 500 mg/kgBW of the extract, indicating its anti-apoptotic effect. These findings suggest that P. foetida leaf extract may serve as a potential therapeutic agent for reducing cell apoptosis in sepsis, warranting further investigation into its mechanisms and clinical pharmacological field.
Edukasi Pembuatan Pakan Silase Ruminansia dengan Kultur Campuran Lactobacillus sp., Rhodopseudomonas sp., Actinomycetes sp., Streptomyces sp., dan Yeast Savitri, Lisa; Prasetyawan, Fendy; Saristiana, Yuneka; Juwita, Syntia Tanu; Yanti, Novirma; Edda, Ilda Rambu
Abdimas Galuh Vol 7, No 1 (2025): Maret 2025
Publisher : Universitas Galuh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25157/ag.v7i1.18152

Abstract

Pada musim kemarau, ketersediaan tumbuhan alami seperti rumput dan kacang-kacangan menurun, menyebabkan kesulitan bagi peternak dalam memenuhi kebutuhan pakan ternak ruminansia. Hal ini dapat berdampak negatif pada kesehatan dan produktivitas hewan ternak. Pakan ternak ruminansia terdiri dari berbagai jenis makanan, terutama tumbuhan, seperti rumput, kacang-kacangan, dan tanaman lain yang kaya serat dan nutrisi. Tumbuhan ini merupakan sumber utama energi bagi hewan ternak ruminansia seperti sapi, domba, dan kambing. Namun, selama musim kemarau, kekurangan tumbuhan menjadi tantangan utama. Salah satu solusi yang diusulkan adalah pembuatan silase. Silase adalah jenis pakan yang dihasilkan dari proses ensilase, di mana tumbuhan seperti rumput diawetkan dengan mengurangi kadar oksigen dan meningkatkan kadar asam laktat. Proses ini membantu mengawetkan nutrisi dalam tumbuhan dan menyediakan pakan tambahan untuk ternak saat musim kemarau. Program pengabdian masyarakat ini bertujuan untuk memberikan edukasi kepada peternak mengenai teknik pembuatan silase ruminansia menggunakan kultur campuran bakteri tertentu. Diharapkan program ini dapat meningkatkan pengetahuan dan keterampilan peternak dalam memproduksi pakan silase untuk memenuhi kebutuhan ternak ruminansia selama musim kemarau. Universitas Kadiri melalui program Pengabdian Masyarakat ini dapat ikut berkontribusi dalam upaya untuk mendukung program pemerintah yaitu “Making Indonesia 4.0” dalam peningkatan kualitas sumber daya manusia (SDM) dan pembangunan ekosistem inovasi. Melalui publikasi ilmiah, panduan, dan peningkatan pemahaman masyarakat, program ini diharapkan dapat memberikan dampak positif bagi peternak dan ekonomi lokal.
Histopathological Evaluation of Stomach Protection by Peperomia pellucida L. in Mice with Gastroenteritis Savitri, Lisa; Krissanjaya, Rochmad; Kasimo, Elfred Rinaldo; Juwita, Syntia Tanu; Prasetyawan, Fendy; Saristiana, Yuneka; Mebung, Konradus Klala; Amanda, Cornelia
Biology, Medicine, & Natural Product Chemistry Vol 14, No 1 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.141.535-540

Abstract

Gastroenteritis, a widespread condition characterized by inflammation of the stomach and intestines, poses significant health challenges globally. Conventional treatments primarily focus on symptomatic relief and do not address the underlying gastric mucosal damage. Peperomia pellucida L., a medicinal plant known for its anti-inflammatory and antioxidant properties, has been suggested to possess gastroprotective effects. This study aims to evaluate the histopathological effects of P. pellucida L. on gastric protection in a mouse gastroenteritis model. Male Swiss mice were divided into six groups, receiving different treatments, including the ethanol extract of P. pellucida at varying doses (100, 300, and 500 mg/kgBW), followed by induction of gastroenteritis with Escherichia coli. Histopathological analysis was conducted to observe tissue damage across the treatment groups, including necrosis and cell degeneration. The results revealed that P. pellucida L. exhibited significant gastroprotective effects, particularly at a dose of 500 mg/kgBW, reducing gastric mucosal necrosis and inflammation compared to the control groups. The plant’s secondary metabolites, including flavonoids, tannins, saponins, and alkaloids, contributed to the observed protective effects by enhancing mucus production, reducing gastric acidity, and promoting tissue repair. These findings suggest that P. pellucida L. could be an alternative treatment for gastroenteritis and related gastric conditions, offering a natural approach to managing gastric inflammation and mucosal protection.
Antibacterial Activity Test of Trump Extract Dendrophtoe petandra (L.) Miq. Against Pseudomonas aeruginosa in Vitro Savitri, Lisa; Alves, Silvina Sandra X.; Hilmi, Mochammad Hanif; Krissanjaya, Rochmad; Prasetyawan, Fendy; Juwita, Syntia Tanu
Biology, Medicine, & Natural Product Chemistry Vol 14, No 1 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.141.553-556

Abstract

Mistletoe (Dendrophthoe pentandra (L.) Miq.) is one of the plants found in Indonesia. The type of mistletoe commonly found is D. pentandra (L.) Miq. Apart from its parasitic nature, the leaves of D. pentandra (L.) Miq. has useful benefits as an antibacterial. Bacteria are divided into two groups based on their staining, namely Gram-positive bacteria and Gram-negative bacteria. Infectious diseases can be caused by several types of microorganisms in the bacterial group, including Pseudomonas aeruginosa. This study aims to determine the P. aeruginosa bacteria in the extract of lime mistletoe. This study used an experimental post-test only control research design with the disc diffusion method. This study used five concentrations, namely 20%, 40%, 60%, 80%, and 100%, and used distilled water as a negative control and ciprofloxacin as a positive control. The inhibition zone is determined by observing the clear zone.
In Vitro Evaluation of the Antibacterial Properties of Lime Mistletoe (Dendrophthoe petandra (L.) Miq.) Extract Against Escherichia coli Savitri, Lisa; Mahardika, Juan Vega; Yanti, Novirma; Juwita, Syntia Tanu
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.641-644

Abstract

The increasing prevalence of antibiotic-resistant bacteria necessitates the exploration of alternative antimicrobial agents derived from natural sources. This study aimed to evaluate the in vitro antibacterial activity of lime mistletoe (Dendrophthoe petandra (L.) Miq.) extract against Escherichia coli ATCC 25922 using the disc diffusion method. Extract concentrations of 20%, 40%, 60%, 80%, and 100% were prepared alongside negative (distilled water) and positive (Cefadroxil) controls. Nutrient Agar (NA) media were inoculated with E. coli and discs impregnated with each concentration were placed on the media, followed by incubation at 37°C for 24 hours. The inhibition zones were measured to assess antibacterial activity. Results demonstrated a concentration-dependent increase in antibacterial efficacy, with inhibition zone diameters ranging from 8.3 mm at 20% extract to 20.3 mm at 100% extract concentration. The highest concentration’s inhibition zone was comparable to that of Cefadroxil (22.5 mm). No inhibition was observed in the negative control. Statistical analysis confirmed significant differences between treatment groups (p < 0.05). The antibacterial activity of lime mistletoe extract is likely due to its bioactive phytochemicals such as flavonoids and phenolics, which are known to disrupt bacterial cell membranes and inhibit microbial growth. These findings suggest that Dendrophthoe petandra extract holds potential as a natural antibacterial agent, providing an alternative to conventional antibiotics amid growing resistance issues. Further studies involving isolation of active compounds, toxicity evaluation, and in vivo testing are recommended to validate its clinical applicability.
Co-Authors Alves, Silvina Sandra X. Amanda, Cornelia Andi Cahyadi Antoro, Ester Lianawati Armayani, Erawati Datin An Nisa Sukmawati Edda, Ilda Rambu Elfred Rinaldo Kasimo, Elfred Rinaldo Fendy Prasetyawan Freitas, João Gregório Freitas, Maria Do Carmo Da Costa Hilmi, Mochammad Hanif Laili, Nur Fahma Mahardika, Juan Vega Mebung, Konradus Klala Neni Probosiwi Nunki, Nastasya Nur‘ Aini, Farida Ornai, Andre Mario Sequeira Alves Rahmi Rusanti, Rahmi Retnowati, Ana Rochmad Krissanjaya savitri, Lisa Tanzilia, May Fanny Tsamrotul Ilmi Wulansari, Ida Septika Yanti, Novirma Yetti Hernaningsih Yuneka Saristiana